Alpha-Synuclein Physiology and Pathology: A Perspective on Cellular Structures and Organelles

1The alpha-synuclein (α-syn) protein is encoded by the SNCA gene localized on the long arm of chromosome 4 (Chr 4q22.1), composed of 140 amino acid residues with a molecular weight of approximately 15 kDa, which presents three domains. The C-terminal region is rich in acid residues, whereas the central region known as the non-amyloid component (NAC) permits the oligomerization of α-syn due to its hydrophobic composition. On the other hand, the N-terminal region contains four regions of 11 imperfect repeats with the KTKGEV consensus sequence. This allows the formation of an alpha helix, which enables lipid-binding; particularly, α-syn binds to negatively charged lipids.

2In its monomeric state, α-syn is intrinsically disordered and soluble, and constitutes the most common form found inside the cytoplasm. The aggregation of several monomers of α-syn gives rise to oligomers, which can adopt different morphologies such as spherical, chain-like, annular (pore-like structure), and tubular. The three-dimensional structure that α-syn can adopt varies from monomers and oligomers to fibrillar conglomerates, the last two linked to cytotoxicity. Similarly, different mutations on the SNCA gene like p.A30P and p.A53T are more prone to the formation of protofibrillar intermediates of α-syn oligomers.

3The aggregation process involving the binding between α-syn and other protein complexes such as tau protein and β-amyloid, termed “cross-seeding,” is the main mechanism for the formation of Lewy bodies (LB), which are abnormal neuronal intracytoplasmic inclusions consisting of more than 70 proteins, whose core is mainly constituted by α-syn fibrillar aggregates. This differs from the formation of fibrillar aggregates from α-syn oligomers called “seeding”. It is thought that α-syn fibrils tend to form due to the interactions between monomers and oligomers that are thermodynamically favorable and stabilizing. The characteristic conformation of α-syn fibrils is β-sheets, with the individual β-strands arranged in parallel.

4The presence and detrimental effects of abnormal cytoplasmatic α-syn accumulation, and their aberrant forms in neurons and glia are linked to neurodegenerative diseases, called synucleinopathies, which includes Parkinson’s disease (PD), dementia with Lewy bodies (DLB), and multiple system atrophy (MSA), the former being the most common synucleinopathy. It is notable that some SNCA gene allelic variants (e.g., SNCA locus triplication) are enough to develop a severe early onset PD and DLB. However, it is remarkable that up to one third of patients with PD are due to LRRK2-gene mutations/PARK8 and almost all patients with Parkin-gene mutations/PARK2 lack LB pathology.

5At the cellular level, native α-syn is present in synaptic terminals, in the nucleus of neuronal cells, mitochondria, endoplasmic reticulum (ER), Golgi apparatus (GA), and in the endolysosomal system. However, its physiological function in each subcellular compartment is only partially understood (summarized in Table 1). At the same time, the presence of the pathologic forms and its effects has been linked to both damaging and protecting outcomes, resulting in controversial conclusions using sinucleinopathies models (also summarized in Table 1). Indeed, cytotoxic effects of α-syn fibrils are related to increased oxidative stress, impaired axonal transport, ubiquitin-proteasome machinery, mitochondrial function, and synaptic dysfunction. These harmful intracellular effects can be facilitated by neuron-to-neuron transmission through direct penetration, synapse–synapse contact, membrane receptors, and endocytosis.

6For the most part, the stimuli that trigger oligomer formation are unknown, although several events have been characterized that seem to favor their appearance, such as an increase in temperature and a decrease in pH. In addition, the incubation of 4-hydroxy-2-non-enal, a product of lipid oxidation and related to neurodegenerative diseases, with human wild-type (hWT) α-syn, prevents its fibrillation but induce β-sheet-rich oligomers. Similar results can be obtained in mesencephalic neuronal cells cultures exposed to polyunsaturated fatty acids. It is suggested that the main route by which oligomers are cytotoxic is by disruption (pore formation) of the plasma membrane of the cells. These pores facilitate the diffusion of molecules and ions, especially Ca2+, which, in high concentrations, is harmful to neurons, inducing cell death.

7Another relevant conformation of α-syn is given by its phosphorylation. Furthermore, the main post-translational modification of α-syn in LB is that more than 90% is phosphorylated in the serine 129 residue (pS129-α-syn), while brains without LB represent less than 4% of total α-syn. Although the kinase responsible for this phosphorylation in the context of LB formation is unknown, several candidate kinases have been proposed to phosphorylate a-syn, including casein kinase 2 (CK2), G protein-coupled receptor kinase 2 (Gprk2), and Polo-like kinase 2 (PLK2).

8It has been found in vitro that CK2, a serine/threonine kinase, may be responsible for cytosolic α-syn and membrane-associated α-syn phosphorylation, since in 3D5 cells exposed to ferrous chloride, there was an increment in α-syn inclusions, along with CK2 up-regulation. Furthermore, the inhibition of this kinase decreased the formation of such inclusions.

9In parallel, in a Drosophila model of PD, the expression of hWT α-syn and Gprk2, which has α-syn as a substrate, leads to an increment of pS129-α-syn and α-syn-mediated toxicity was observed. There is also evidence that Gprk family mediated phosphorylation occurs only in membrane-associated α-syn.

10Besides phosphorylation of the serine 129 residue mediated by PLK2, a serine/threonine kinase could also be involved in a mechanism of α-syn clearance by the lysosome–autophagic degradation pathway. PLK2 overexpression in HEK cells decreases the amount of α-syn that correlates with the amount of PLK2 and pS129-α-syn. Also, when 3MA (a lysosomal pathway blocker) is added, the amount of α-syn returns to baseline levels.

11Among the functions attributable to pS129-α-syn are as a regulator of dopamine uptake; in SH-SY5Y and HEK293 cells, the membrane-bound pS129-α-syn is associated with increased expression of DAT on the cell surface. Furthermore, the phosphorylation of pS129-α-syn might also be a mechanism whereby the cell reduces the damage caused by the formation of pores caused by alpha-synuclein oligomers. In unilamellar lipid vesicles, it has been shown that pS129-α-syn inhibits the binding of α-syn oligomers to the membrane and therefore decreases the pore formation.

12Despite this evidence, it remains to be clarified what is the role of pS129-α-syn. It seems that in early stages of α-syn accumulation and formation of α-syn oligomers, the pS129-α-syn may act like a compensatory mechanism, but in later stages, pS129-α-syn could mediate neurotoxic events. Furthermore, the phosphorylation observed in fibrils might be a result, not a catalyst, of the aggregation process. For these reasons, it has been postulated that LB could have a cytoprotective role; however, current evidence is still controversial.

13In this review, we discuss current evidence related to α-syn as a prion-like protein, the interactions between α-syn with soluble and insoluble proteins, as well as the function of α-syn within different organelles. We also include data on human-induced pluripotent stem cells (hiPSC), derived from PD patients, since α-syn is a ubiquitous protein that, when it is present in monomeric form, performs physiological functions (e.g., regulation of mitochondrial fusion–fission), but when grouped in toxic conformations (e.g., oligomers or fibrils) is related to organelle dysfunction (e.g., mitochondrial and GA fragmentation) that ultimately lead to pathological processes.

14A prion is a protein with aberrant folding, inducing aggregation by physically interacting with other proteins. Through this process, it generates a great quantity of misfolded proteins, which are pathological for the carrier (patient). Nevertheless, a fundamental characteristic of prion proteins is their inter-organism transmissibility, causing the same phenotype, making prions infectious agents with high morbidity. The fact that α-syn aggregates have been found in different regions of the brains of PD patients depending of the disease stage, evidence of α-syn propagation from the gut to the brain, and the finding of LB in grafted fetal midbrain neurons into the striatum of PD patients over 10 years after transplantation have led several groups of researchers to conclude that α-syn acts as a prion-like protein. But so far, no evidence has been found supporting its transmissibility from one organism to another. Therefore, in PD context, α-syn cannot be tagged as a prion protein, but it can be studied more as a prionoid or prion-like protein. Interestingly, MSA, another synucleinopathy, is classified as a prion disease due to the evidence that inoculation of samples from brains of MSA patients into p.A53T α-syn transgenic mice brains causes aggregation and spreading of α-syn, in addition to a MSA-like phenotype. Indeed, these results can be replicated by inoculating homogenate from the first transplanted rat brain into a second rat brain. Similar results can be found when the rat is exposed peripherally (like peritoneal cavity) to the homogenates of MSA brains. Nevertheless, some issues need to be taken into account, like the fact that these results could not be obtained in wild-type (WT) mice. In relation to DLB, more information is needed to know if it has a transmission mechanism like prions.

15As mentioned earlier, α-syn contains an N-terminal domain, which permits its interaction with membrane lipids, and both the pS129-α-syn and α-syn phosphorylated at tyrosine 125 (pY125-α-syn) represent pathological forms of α-syn. In order to unravel the interactions of phosphorylated and non-phosphorylated α-syn, performed a comparative proteomic approach. Peptides containing 40 amino acids of the C-terminal domain of hWT α-syn and a phosphomimic form, which consisted in the substitution of the serine 129 for aspartic acid, were used. Mass spectrometric analysis of solubilized mouse brain synaptosome proteins, pulled down with the peptides, allowed the observation that the phosphorylated α-syn peptide could interact with cytoskeletal and vesicular traffic proteins, as well as serine protein kinases, whereas non-phosphorylated α-syn could not. On the contrary, with the same peptides, non-phosphorylated α-syn showed interaction with oxidative phosphorylation (OXPHOS) proteins, but this interaction was not observed for phosphorylated α-syn.

16To highlight the multiple interactions of α-syn, we used a platform of predicted interactions of proteins (STRING, ) and a dataset of reported information from individual studies (BioGRID3.5, ). For the STRING analysis (Figure 1A), we used SNCA as a key word and Homo sapiens as organism of search. These analyses were performed establishing 20 interactors instead of the 10 showed by default in the program, in order to have a greater landscape of its interactions and 0.400 was used as the interaction score, which is a threshold on the confidence score, such that only interactions above this score were included in the network. The STRING analysis showed that α-syn presented greater interaction with synphilin-1, which has been reported to bind to vesicle membranes of the presynaptic terminals and interacts with α-syn to inhibit the docking of the fusion vesicles. This suggests that synphilin-1 mediates the synaptic function of α-syn in HEK293 cells. The interaction between synphilin-1 and α-syn was also observed in a yeast model with the same effect of inhibition in the docking of the fusion vesicles; however, the function of synphilin-1 needs further research (Figure 1A). In addition, in our analyses, α-syn interacts with kinase proteins like LRKK2 and Fyn, as well as ubiquitin ligases like PARK2 and PARK7 and STUB1, which are reported to be involved in PD.

17For the BioGRID3.5 analyses (Figure 1B), we used SNCA as a keyword and H. sapiens as organism of search. In the BioGRID3.5 analyzes, it was found that α-syn showed 69 interactor proteins, which included kinase proteins such as Pink1, involved in mitochondrial function; LRKK2, which mediates assembly of the cytoskeleton; MAPK1, implicated in many cell functions like cell adhesion, cell cycle progression, cell differentiation, among others; and SEPT4, which is a protein with a nucleotide-binding domain that regulates cytoskeletal organization. Other proteins that interact with α-syn are ubiquitin ligases like STUB1, involved in cell death regulation; Park2, which ubiquitinates Bax protein in mitochondria and regulates apoptosis; and NEDD4, which ubiquitinates α-syn to lead its degradation. Extensive reviews about experimental evidence of the interaction between α-syn and proteins can be found elsewhere.

18The closest interactions to α-syn found in both STRING and BioGRID3.5 analyses showed soluble protein interactors that are involved in cellular process such as degradation (Figure 1). However, it has been determined that α-syn can also bind to insoluble proteins, which are localized in membranous compartments of the cells. Furthermore, some of these proteins are voltage-dependent anion channel (VDAC), translocase of the outer membrane 20 (Tom20), and adenylate translocator in mitochondria (Figure 2). α-syn can also be localized with importin alpha in the nucleus (Figure 3), sarco/ER Ca2+-ATPase (SERCA) in the ER (Figure 3), and Rab1A both in the ER and the endolysosomal system (Figure 3). The interactions between α-syn and the proteins mentioned are described below.

19The mitochondria is a double-membrane-bound organelle involved in oxidation of metabolites, generation of adenosine triphosphate (ATP) through OXPHOS, Ca2+ signaling, and apoptosis. α-syn has a higher affinity for mitochondrial membranes compared to lipid membranes of the other organelles. α-syn presence was determined by Western blot in the isolated mitochondrial and cytosolic fractions of WT rat brains, whereas in humans, the relationship between α-syn with mitochondria was determined due to the pathological findings of α-syn accumulation within the mitochondria of brain regions like the substantia nigra (SN) and the striatum of PD patients, although mitochondrial dysfunction was a well-known phenomenon in PD since the 1980s.

20Specifically, α-syn has been reported in the three main mitochondrial structures: the inner mitochondrial membrane (IMM), the outer mitochondrial membrane (OMM), and the mitochondrial matrix (MM). Interestingly, pS129-α-syn has a higher binding affinity to the OMM than the WT α-syn. However, the same is not applicable for the ER and the GA.

21The first 32 amino acids of the N-terminal of the α-syn are necessary for its translocation to mitochondria and α-syn can be internalized without the need of post-translational modifications or that has been mobilized first through another organelle. It has a role as a regulator in some of the mitochondrial physiological functions like mitochondrial fusion, electron transport chain (ETC), and VDAC permeability, among others (Figure 2).

22showed results of a protective role with low and physiological concentrations of α-syn (0.1–1.0 μM) against oxidative stress in cortical and hippocampal neurons of rats and cell cultures of LHRH (luteinizing hormone-releasing hormone)-secreting neurons, SH-SY5Y neuroblastoma cells, and pheochromocytoma (PC-12) cells. Nevertheless, α-syn accumulation, overexpression, or impairment of its degradation mediates mitochondrial dysfunction that can lead to more α-syn accumulation, misfolding, and inclusions. Although the molecular mechanisms of these phenomena are not completely understood, it is postulated that α-syn oligomers form covalent cross-links between tyrosine residues under oxidative conditions, giving rise to larger filaments, and these α-syn conformations can increase reactive oxidative species (ROS) production by interacting with ETC elements, resulting in a cycle where the aggregation and generation of ROS exacerbates each other (Figure 2).

23Related with the above, the overexpression of hWT α-syn in Caenorhabditis elegans body wall muscle accelerated age-related mitochondrial morphological changes (Table 1). Furthermore, in mice overexpressing hWT α-syn, mitochondrial dysfunction in dopaminergic neurons from the nigrostriatal pathway preceded several months before depletion of striatal dopamine.

24Mitochondrial fusion is a process by which mitochondria can mix their content, homogenizing it and avoiding loss of essential material like mitochondrial DNA (mtDNA) localized in the MM. Impairment of mitochondrial fusion is linked to neurodegenerative diseases. A recent study found that the overexpression of α-syn in neurons can confer protection against ROS, since it decreases a type of dynamin-related protein 1 (Drp1)-dependent mitochondrial fragmentation related to H2O2 and 6-hydroxydopamine, known as mitosphere (hyperpolarized mitochondria with a spherical form), which precedes the activation of apoptosis mediated by Caspase 3. However, further research is needed in order to know the α-syn baseline concentration contribution in mesencephalic dopaminergic neurons, in order to understand the importance of α-syn in the most affected cell type, which indeed has a high rate of ROS.

25Alpha-synuclein could, in part, act like a physiological regulator of mitochondrial fusion. Indeed, mitochondria need to be smaller in size (fission) in order to be mobilized through axons of dopaminergic neurons, as they have a high energy expenditure and therefore need a large number of mitochondria in synaptic terminals. Recent evidence suggests that α-syn inhibits mitochondrial fusion proteins like mitofusin-1 (Mfn1), mitofusin-2 (Mfn2), and optic atrophy type 1 (Opa1). Furthermore, α-syn binds to curved membrane sites on the OMM, blocking the fusion stalk buildup, a necessary structure for mitochondrial fusion, thus favoring mitochondrial fission. However, α-syn accumulation inhibits mitochondrial fusion proteins and lowers the proportion of fusion stalks that form correctly, hence promoting mitochondrial fragmentation (Figure 2).

26In agreement with the above, HeLa cells overexpressing α-syn exhibit increased mitochondrial fragmentation (independent of intrinsic fusion proteins, e.g., Mfn1, Mfn2, Opa1 and fission proteins, e.g., Drp1) that preceded mitochondrial function impairment. Similar results were found in SH−SY5Y cells expressing hWT or p.A53T α-syn, but the same research described that both spinal cord and cortex neurons of mice that express mutant p.A53T α-syn also had an age−dependent manner of mitochondrial morphological changes (e.g., decreased of mitochondrial connectivity, related to mitochondrial fragmentation and increased in circularity, which is in turn linked to reduced mitochondrial length) together with a decrease in fusion–fission proteins. The impairment was attributable to Mfn2 decrease, since expression of this protein in SH−SY5Y cells rescued the phenotype. These results suggest that WT and mutated forms of α-syn have similar implications on mitochondrial morphology, but through different mechanisms.

27Although mitochondria have their own DNA, they only contain genetic coding information for 13 proteins, and most mitochondrial proteins are nuclear encoded. Thus, mitochondria need to import more than 99% proteins from the cytoplasm for its function, which is facilitated by transport systems like TOM, a protein complex located in the OMM, allowing the translocation of proteins from the cytosol to the mitochondrial intermembrane space. TOM is composed of seven proteins (Tom5–7, Tom20, Tom22, Tom40, and Tom70), and the proteins to be translocated to the mitochondrial intermembrane space contain specific mitochondrial targeting signal (MTS) recognized by Tom20 and Tom22, whereas Tom40 forms a β-barrel protein-conducting channel.

28Recent studies using the in situ proximity ligation assay (PLA) in SH-SY5Y cell cultures and fluorescence resonance energy transfer (FRET) in rat brain mitochondria have shown that small oligomers of α-syn, pS129-α-syn, and dopamine-modified α-syn interact with the MTS recognition site on Tom20, blocking the Tom20–Tom22 interaction, necessary for protein import, resulting in diminished protein import. In contrast, monomeric α-syn, nitrated α-syn, and thioflavin T–positive α-syn fibrils do not affect the TOM system. The impairment in protein import has consequences for key mitochondrial proteins imported from the cytosol, for example, the lack of NADH:ubiquinone oxidoreductase core subunit S3 (Ndufs3), a complex I subunit of the ETC, led to metabolic deficits and excessive production of ROS (Figure 2). This phenotype could be prevented by Tom20 overexpression.

29Reduced levels of Tom40 have also been found in hWT α-syn overexpression transgenic mice models and in the midbrain of postmortem patients with PD; similar to Tom20, Tom40 overexpression in mice decreased α-syn-induced mitochondrial dysfunction. It is noteworthy that, when α-syn is phosphorylated (pS129-α-syn), it reduces its interaction with Tom40.

30On the other hand, VDAC is a pore with a β-barrel structure located in the OMM. It accounts for about 10% of the OMM total protein and it allows the diffusion of ions, metabolites, and polypeptides in an alternated open/close state voltage-dependent manner. Like the TOM complex, there is evidence demonstrating that α-syn interacts reversibly with VDAC in a concentration-voltage-dependent manner (Figure 1); besides, VDAC can translocate α-syn into the mitochondria, where it can interact with complex I of the ETC and contribute to mitochondrial dysfunction. At nanomolar concentrations (50 nm) of α-syn, its negatively charged C-terminal region interacts with the positively charged pore of VDAC, creating a steric block for flow through the pore. For example, the ATP/ADP exchange between cytosol and intermembrane space could be altered, resulting in diminished ATP synthase activity, decreased membrane potential, and impairment of OXPHOS (Figure 2).

31Therefore, the alterations caused by the α-syn on TOM complex (prevent the binding of MTS to Tom20) and VDAC (steric pore blockage), the most important systems in the translocation of cytoplasmic proteins to the mitochondria, have repercussions both in bioenergetics and in the maintenance of structures of this organelle, which can contribute to mitochondrial dysfunction that are seen in synucleinopathies.

32Cardiolipin is an anionic phospholipid formed by the union of two phosphatidylglycerol molecules with a glycerol molecule located mainly in the bacterial plasmatic membrane and in the IMM. It is well established that α-syn can interact with cardiolipin through its N-terminal KAKEGVVAAAE repeats, specifically with cardiolipin acyl side chains via electrostatic interactions. The OMM has low concentrations of cardiolipin; however, externalization of cardiolipin from the IMM is stimulated under stress conditions, mitochondrial morphology alterations, pro-mitophagy conditions, and low pH, where it is necessary for (1) the recruitment of LC3 (autophagy protein microtubule-associated-protein-1 light chain 3), a required protein for the binding of the phagophore membrane to the impairing mitochondria, and (2) the binding of pro-apoptotic molecules like caspase-8, Bax, Bak, and tBid. α-syn may regulate the LC3-induced mitophagy as they compete for the same binding site on such lipid.

33The same study using hiPSC carrying an SNCA p.A53T mutation and its generated gene-corrected isogenic hiPSC line has shown that cardiolipin in the OMM can bind to mutant α-syn to fold it into a α-helix conformation. Similarly, cardiolipin reverses α-syn oligomeric fibrils in aggregated β-sheet α-syn monomers, and from this conformation, it can turn into α-syn monomers with α-helix structure (Figure 2). This allows mitochondria to have the capacity to buffer toxic forms of α-syn. However, recent evidence shows that α-syn oligomers have a greater propensity to form pores in cardiolipin-rich membranes, which could contribute to the mitochondrial dysfunction.

34These findings demonstrate that, through the translocation of cardiolipin, mitochondria are able to buffer the pathological forms of α-syn. However, it can also have a detrimental effect because it facilitates the formation of membrane pores that can lead to mitochondrial dysfunction. Therefore, further studies are needed to determine which effect has a greater preponderance.

35In mitochondria, the IMM has a high α-syn concentration determined by immunoelectron microscopy and Western blot analysis, to which it binds in an α-helical conformation. The high concentration of α-syn in the IMM may be due to the cardiolipin content in this structure and the fact that α-syn interacts with many components of the ETC–OXPHOS.

36The ETC is formed by four complexes (CI–CIV) located in the IMM, whose function is the coupling between the transport of electrons between the complexes and the pumping of protons, creating an electrochemical gradient between the mitochondrial matrix and the intermembrane space, for the synthesis of ATP by the ATP synthase. Complex I or NADH:ubiquinone oxidoreductase accepts the electrons from NADH and delivers it to Complex III through the electron-carrier ubiquinone. It also pumps protons from the mitochondrial matrix to intermembrane space.

37Evidences that link α-syn physiological role to ETC is that, first, α-syn knockdown cells have a concentration and activity reduction in NADH cytochrome C reductase, an event related to complex I/III activities, which may be due to a change in lipid metabolism caused by the α-syn deficiency, and second, the fact that triple synuclein knockout (α-, β-, and γ-syn) is resistant against complex I inhibitor rotenone. It has also been proposed that detrimental effects (e.g., increased in ROS production, decreased ATP synthesis) on complex I activity are due to an exacerbation of their likely physiological role (not yet elucidated) as a negative regulator of complex I, which eventually leads to mitochondrial impairment (Figure 2).

38Accordingly, the pathological effects of α-syn causes down-regulation of complex I activity, which in turn lead to ETC impairment (especially in ventral midbrain), decreased ATP synthesis, and increased mitophagy and ROS production (mainly superoxide and H2O2), a well-known promoter of α-syn oligomerization (Figure 2).

39The fact that the presence and accumulation of α-syn (either hWT or pathological forms) lead to alterations carried out by the complex I makes it an important element of mitochondrial dysfunction, due to the fact that it can trigger and perpetuate other events harmful to the mitochondria and the cell (e.g., cycles where α-syn favors the production of ROS and these favor the aggregative forms of α-syn and vice versa). Therefore, complex I can be an element to consider as a therapeutic target.

40The ROS production by overexpression of hWT α-syn in mouse models has been postulated as one of the causes of mtDNA damage, especially somatic mtDNA deletions that may lead to an altered expression of mitochondrial genome-encoded genes, like complex I and complex IV subunits, which would increase the damage by ETC and OXPHOS (Figure 2). There is also evidence that the prevalence of mtDNA mutations increases with age, predominantly in vulnerable cells like dopaminergic neurons and regions like caudate and putamen (in comparison to cells from cerebral cortex, cerebellum, and dentate nucleus), and are higher in PD patients. As mentioned above, mtDNA mutations are related to low expression of mtDNA-encoded proteins (e.g., subunits of complex IV). Interestingly, the number of copies of mtDNA increases with age, which is thought to be a compensatory mechanism to age-related mutations in mtDNA. However, this mechanism is diminished in patients with PD.

41Adenosine triphosphate synthase uses the electrochemical gradient generated by the proton pumping of the ETC for the phosphorylation of ADP to form ATP. It has also been shown through PLA assays that α-syn monomers interact with the ATP synthase α-subunit, which suggests that α-syn acts as a regulator of the ATP synthase. Triple α-, β-, and γ-syn knockout midbrain neuronal cells of mice have lower mitochondrial membrane potential and uncoupling between OXPHOS and respiration, which leads to a decrease in ATP synthase activity due to low respiratory control ratio values. Monomeric α-syn rescues this phenotype. In contrast, another study found that α-syn knockout mouse neurons do not exhibit alterations in mitochondrial bioenergetics.

42On the other hand, α-syn oligomers have an opposite effect on ATP synthesis. The ROS produced by these toxic forms of α-syn generate lipid peroxidation and oxidize the ATP synthase β subunit, which impairs its function, thus leading to alterations in OXPHOS, due to the depolarization of the membrane. These oxidative events can stimulate the opening of the permeability transition pore (PTP).

43The hWT α-syn interacts directly with an adenylate translocator and VDAC, both part of the PTP. The opening of the PTP allows the diffusion of molecules of <1.5 kDa and ions, including Ca2+, resulting in membrane depolarization and mitochondrial swelling that leads to OMM rupture with concomitant cytochrome C leak, events that trigger cell death. In the same manner, hiPSC carrying α-syn overexpression due to a genomic triplication of the SNCA locus and human embryonic stem cells (hESC)-overexpressing α-syn derived neurons exhibit a decrease in calcium-induced PTP threshold opening, which can lead to increased vulnerability and cell death (Figure 2).

44As seen previously stated, ROS generated by α-syn affects multiple mitochondrial elements; in this case, ROS can trigger apoptosis due to oxidizing and stimulating of PTP elements. Additionally, α-syn can lower the threshold of activation of PTP, which increases these phenomena. This pathological mechanism may be one of the pathways through which α-syn can lead to mesencephalic dopaminergic neurons to death.

45For all the above, mitochondria have been postulated as the central organelle affected in synucleinopathies, although, as will be discussed in the upcoming sections, other organelles could also play a fundamental role in the pathological development.

46The name syn-nuclein indicates synaptic and nuclear distribution. Although the synaptic function of α-syn is widely studied, particularly the mechanisms involved in vesicular transport, its function within the nucleus is poorly understood. Interestingly, α-syn appears to modulate the physical properties of DNA. Using WT α-syn on nanofluids with DNA, found that its binding was, to a large extent, driven by electrostatic interactions and gradually stiffened as α-syn was binding to naked DNA, resulting in gradual increase in DNA length.

47One of the key aspects of the interaction between α-syn and the nucleus is its importation from the cytoplasm. A recent report has described the critical role of the nuclear pore complex for the mobilization of α-syn through the nuclear membrane. By tagging both α-syn and karyopherin alpha 6 (a nuclear adaptor protein) in SH-SY5Y cells and mouse fetal primary cortical neurons, and evaluating their physical interaction using FRET, it was found that α-syn interacts with karyopherin alpha 6 after α-syn has been sumoylated, a mandatory process for its transport to the nucleus.

48Furthermore, various transcripts of α-syn with different deletions were expressed on PC-12 cells and followed through its mobilization from the cytoplasm to the nucleus. With this model, found that the C-terminus of α-syn (residues of amino acids 103–140) appeared to play an important role in its nuclear accumulation. Apparently, the protein forms a specific spatial structure based on C-terminus interactions with the central part, necessary to join together; this may allow for the nuclear accumulation of α-syn. Moreover, the nuclear accumulation of α-syn is mediated by importin alpha, but the results obtained suggest that an unknown protein mediates the direct interaction between α-syn and importin. No further information regarding the missing protein has been reported yet.

49The interactions that α-syn can have in the nucleus are not restricted to DNA. It has been found that α-syn fibrillation is accelerated in the presence of histone H1 and core histones. In addition, Paraquat-induced injury in mice increases α-syn concentration in the nuclei of the midbrain. Indeed, the signal of histone H1 colocalized with α-syn and also with H3 and NeuN (neuronal nuclei marker). Therefore, it was suggested that the colocalization observed might be an indication of a complex formation between α-syn and histone H1 and/or H3 in the nucleus.

50Histone condensation is largely responsible for the formation of heterochromatin and euchromatin, the first being transcriptionally repressed and the latter being transcriptionally active. This transcription apparatus is partially dictated by biochemical changes such as acetylation, methylation, or phosphorylation on DNA itself or on histone residues. When the charge of the histone tails is changed by these modifications, they can either lose or gain affinity for DNA and for other histones, allowing or restricting the entrance of transcription factors and other transcription-associated proteins. The proteins responsible for “writing” these marks are, among others, DNA methyltransferases, histone acetyltransferase, and histone methyltransferases, and its corresponding “erasers” are histone deacetylases (HDACs). An extensive review on epigenetic markers can be found in.

51Alpha-synuclein interacts with epigenetic “writers.” It has been observed that in transgenic flies expressing hWT α-syn ubiquitously under control of the daG32-GAL4 promoter, an increase in histone-H3 lysine-9 (H3K9) methylation was present. Furthermore, by overexpressing hWT α-syn in rat B103 neuronal cells, found that α-syn can retain DNA methyltransferase 1 in the cytoplasm of neuronal cells compromising DNA methylation (Figure 3). Indeed, lower levels of methylation were found in intron 1 of the SNCA gene. Moreover, in dopaminergic-like cells differentiated from SH-SY5Y cells with inducible hWT α-syn expression, higher levels of H3K9 mono- and dimethylation were observed (Figure 3), probably affecting mRNA expression of the neural cell adhesion molecule L1CAM and the synaptosomal-associated protein SNAP25, as well as H3K9me2 at the SNAP25 promoter. This might act within a feedback network to tone the release of synaptic vesicles, and this mechanism could derail and contribute to synaptic dysfunction occurring in PD.

52Alpha-synuclein can also interact with epigenetic “erasers.” Substantial evidence has shown that α-syn restricts and maintains HDACs on the cytoplasm, inhibiting its normal function. Indeed, human mutant p.A30P and p.A53T α-syn in SH-SY5Y cells bind to histones, which in turn lowers histone acetylation, a key mark for transcriptional activation (Figure 3). Consequently, inhibiting HDACs protects against α-syn neurotoxicity, as probed in transgenic flies and in SH-SY5Y cells.

53Alpha-synuclein is heavily involved with histone deacetylase 4 (HDAC4), another important epigenetic eraser that is highly expressed in neurons and forms part of the HDACs class IIa that can shuttle between the cytoplasm and nucleus. Indeed, by exposing p.A53T-mutant α-syn mice or PC-12 cells to a sub-toxic concentration of MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine), HDAC4 nuclear accumulation was induced. Furthermore, HDAC4 localized in the nucleus and mediated cell death in p.A53T cells by repressing the transcriptional activity of CREB (cAMP response element binding protein) and myocyte enhancer factor 2A (MEF2A).

54Finally, on H4 cells, it has been reported that the pS129-α-syn form has an affinity for the nucleus, down-regulating important cell-cycle genes like CCNB1 and E2F8, indicating a plausible effect on progression between cell-cycle phases. In addition, showed that pS129-α-syn is rapidly recruited to laser-induced DNA damage sites in the nucleus of in vivo mouse brain cells, as well as in a mouse primary cortical neuron system, having a plausible role on double-strand break repair. This pathological form has also been observed within the nucleus of neurons in brain areas such as the basolateral amygdala, cortex, and hippocampus from aged p.A30P α-syn mice with impaired cognitive behavioral phenotypes. In contrast, these changes were not found in healthy young p.A30P α-syn mice. Thus, phosphorylation of α-syn might also be linked to an aging process.

55In summary, although α-syn physiological role within the nucleus has not yet been completely enlightened, the study of its interactions with nuclear regulatory elements has re-emerged in light of the current advances concerning epigenetic mechanisms, particularly the pathological repercussions involving PD. Indeed, α-syn appears to affect transcriptional regulation by physically interacting with both epigenetic writers and erasers. This can result in its own transcriptional up-regulation, thus promoting accumulation and, in consequence, α-syn aggregation within the cell. This in turn affects the normal function of other organelles compromising cell viability. Therefore, searching for mechanisms by which α-syn transcription can be down-regulated to avoid its aggregation may prevent or diminish neuronal death.

56Another mechanism involved in the synucleinopathies includes vesicular trafficking, a critical system for cellular architecture and communication between organelles within the cell. It is important to highlight that the main organelles that participate in this process are the ER, the GA, and the endolysosomal system.

57The main function of the ER is to synthesize both lipids and proteins, as well as protein folding and post-translational modification of proteins for protein maturation. Furthermore, the ER works as an intracellular Ca2+ reservoir that maintains an oxidative environment and the adequate micromolar concentration of free Ca2+ (0.05–0.1 μM). The ER has also been implicated in sensing the quantity of misfolded or unfolded proteins when there is an insufficiency in the protein-folding capacity of the ER. Hence, in an increase in misfolded proteins, the ER activates the system of unfolded protein response (UPR) and regulates the correct folding or the degradation of proteins.

58In experiments with yeasts, the UPR system is activated in basal conditions but, under the overexpression of α-syn, leads to the overactivation of the UPR inducing ER stress, whereas, in SH-SY5Y cells, the pS129-α-syn causes cell death through the overactivation of the UPR system (Figure 3). In hiPSC-derived neurons, there are no impairments shown in the ER. In contrast, when α-syn is overexpressed due to a SNCA genomic triplication, ER stress has been observed. This promotes the activation of the UPR through the induction of the IRE1α/XBP1 (inositol-requiring transmembrane kinase/endoribonuclease 1α/X-box binding protein 1) pathway, which is a signaling cascade involved in survival signaling under the UPR condition (Table 1). This system is initiated by IRE1α activation, which cleaves XBP1 mRNA leading to its transcriptional function. XBP1 then binds to unfolded protein response element (UPRE) and up-regulates genes encoding ER chaperones to enhance the folding efficiency and the degradation mechanism. The process mentioned before is important due to the accumulation of poly-ubiquitinated proteins like α-syn and other cytoskeleton proteins in histopathological brain studies from PD patients, where UPR was found to be active in response to ER stress. This indicates that this function could be induced to prevent the cytotoxicity associated with ER stress and unfolded proteins.

59In recent studies, it has been demonstrated in dopaminergic (CATH.a) and PC-12 cells, the expression of ER stress markers like GRP78 (78-kDa glucose-regulated protein, chaperone), IRE1α, and phosphorylated eukaryotic initiation factor 2α (peIF2α) is present in basal conditions. However, the overexpression α-syn mutant variants (p.A53T or p.A30P) in cells leads to an increase of these ER stress markers, resulting in apoptosis caused by the loss of the calcium homeostasis, through the action of stress-induced proteins like Herp (homocysteine-induced ER protein). Likewise, the aggregates of α-syn, but not the monomers, can bind and activate SERCA, decreasing the cytosolic Ca2+ concentration that leads to cellular apoptosis in C. elegans and SH-SY5Y cells (Figure 3). On the other hand, the expression of α-syn mutants (p.A53T or p.A30P) promotes its oligomerization in transgenic mice. These oligomeric forms are involved in the pathogenesis of PD, and they could be accumulated within the ER by chronic stress in this organelle.

60Mitochondria-associated ER membranes (MAM) are interconnected by a section from the mitochondrial surface that is proximal to the ER membrane. MAM can regulate cellular processes that directly communicate mitochondria and the ER like calcium homeostasis, lipid metabolism, ATP production, transport and biogenesis of mitochondria, ER stress, response to unfolded proteins, and autophagy. A recent study in BE(2)-M17 neuroblastoma cells showed that mutations in p.A53T or p.A30P can lead to α-syn re-location from the cytoplasm to the vicinity of MAM, mainly altering calcium homeostasis that results in neuronal cytotoxicity. Moreover, found that pS129-α-syn colocalized with Tom20 (a marker of mitochondria) and GRP78, which means that it was localized in the vicinity of MAM. According to these authors, it could prevent the fusion of the mitochondria, as well as alter functions from the ER.

61ELO1, ELO2, and ELO3 are genes that code to three lipid elongases that are involved in fatty acid elongation within the ER. In Saccharomyces cerevisiae, these genes promote a regular growth, while null mutations in these genes show normal growth. Additionally, the null expression of ELO1, ELO2, and ELO3 concomitant to the expression of hWT α-syn, p.A53T, or p.E46K through plasmids in this model showed a defect in cellular growth, as well as aberrant vesicular traffic from the ER to the GA, and ROS accumulation in the cytosol, thus indicating that the inhibition in the synthesis of very long fatty acids promotes the toxicity of α-syn.

62Recently, found that mice with normal expression of α-syn did not present changes in transcriptional regulation. Nevertheless, transgenic mice with expression of hWT α-syn and the p.A30P mutation showed 18 and 2165 genes deregulated, respectively, compared to control mice that just expressed mouse α-syn. In the context of the ER, COL4A2 is an important gene that has been found up-regulated. This gene has also been related to increased levels of ER stress when it has an abnormal expression. In the same study, Lund human mesencephalic (LUHMES) cells that express hWT α-syn did not present significant COL4A2 expression. However, LUHMES cells with p.A30P mutation showed ER stress, reflected in an increase in levels of COL4A2 and CALNEXIN.

63Alpha-synuclein is also implicated in ER-GA vesicular trafficking, regulated by the small guanosine triphosphatases (GTPase) called Rabs. Rabs are hydrolase enzymes that produce guanosine diphosphate (GDP) by the hydrolysis of guanosine triphosphate (GTP), being an important molecular switch in vesicular traffic. In hiPSC-derived midbrain dopaminergic neurons, Rab1A is localized in the ER-GA proximity near the perinuclear position, but when hWT α-syn is overexpressed using a lentivirus, Rab1A has a diffuse localization with an ER-GA fragmentation and disruption of the ER-GA traffic. However, the mechanism by which this occurs has not been studied.

64At the same time, retention in ER sorting receptor 1 (RER1) is a protein involved in the retention of proteins in the ER and retrieval of ER membrane proteins from the GA, also mediating ER-Golgi trafficking. Recently, found that α-syn interacts with RER1 and showed that overexpression of RER1 can reduce α-syn levels of HEK293 in H4 cells. also found that α-syn is ubiquitinated by NEDD4, an E3 ligase, which interacts with RER1 to induce α-syn degradation by the proteasome system. Furthermore, in histopathological brain studies from DLB, RER1 has been located in LB-like round structures, presenting colocalization with pS129-α-syn, unlike control brains; indicating the relevance of RER1 in regulating α-syn activity during vesicular trafficking.

65For all the above, it is clear that α-syn overexpression, its mutants or pathological forms, can induce stress in the ER conducting at its malfunctioning. Furthermore, growing evidence indicates that α-syn interacts with Rab1 and RER1. Moreover, RER1 appears to regulate α-syn levels. Thus, by elucidating the role of RER1 with α-syn and how to regulate its levels, α-syn cellular concentrations could also be regulated.

66The GA is an organelle formed by a membranous cisterna, in which the proteins emerging from the ER are fully processed and sorted to different cellular destinations. The series of cisternae is divided into cis, medial, and trans Golgi compartments, assembling the cis Golgi network (CGN) and the trans Golgi network (TGN). In the CGN, proteins undergo modifications, whereas in the TGN, the modified proteins are packed and sorted for delivery to the lysosomes, plasma membrane, or secretory vesicles.

67The specific physiological functions of α-syn in GA are still unknown. However, the GA has been shown to have impaired ionic transport and membrane traffic as well as deficits of axonal transport associated to GA fragmentation.

68Among the observed alterations of the GA on synucleinopathies is the calcium-transporting ATPase 1 (PMR1) pump, which regulates intracellular levels of Ca2+ and Mn+2 ions in the GA. In neurotoxic conditions such as α-syn accumulation, a decrease in cell death has been observed due to the prevention of the intracellular Ca2+ overload, a mechanism performed by the PMR1 pump in C. elegans. Nonetheless, in models of PD (yeast, flies, and nematodes), PMR1 has been linked to α-syn toxicity by increasing Ca2+ levels and the depletion of PMR1 decreases cell death. Thus, PMR1 could have significant relevance in the development of the α-synucleinopathy as a therapeutic target, although more research on mammal models for PMR1 is required in order to develop any type of treatment.

69Regarding the role of α-syn in GA fragmentation, found GA with an apparent breakdown and diffusely distributed in primary rat astrocytes that had an overexpression of WT α-syn or p.A30P and p.A53T mutants. Furthermore, in the study described in the ER section, found that control LUHMES cells (constitutive expression of α-syn) had a normal morphology of GA in contrast to cells with exogenous expression of hWT α-syn and the p.A30P mutation that showed GA fragmentation. However, the percentage with diffuse morphology was higher in cells with the p.A30P mutation compared to hWT α-syn. Additionally, in a study in COS-7 cells that express α-syn using a recombinant adeno-associated virus vector (SNCA AAV vector), GA morphology was normal in cells with diffuse staining of α-syn, but when the cells had prefibrillar α-syn, fragmentation and dispersion of GA were shown. Indeed, in cells with fibrillar inclusions, there was no apparent association to GA fragmentation, which suggests an early event before fibrillar aggregation of α-syn in COS-7 cells.

70A hypothesis for GA fragmentation is the impairment in microtubules that had been related to the maintenance of the structure of this organelle. In this sense, it has been found that in SH-SY5Y cells, the normal juxtanuclear structure of GA was altered in a similar pattern in both cells exposed to drug-induced depolymerization of microtubules and in cells with α-syn overexpression. Additionally, α-syn overexpression affected the transport mechanism dependent on microtubules.

71Furthermore, other studies on GA fragmentation have found some alterations and possible interactions of α-syn. found that in contrast to COS-7 cells with constitutive expression of α-syn, and in conditions of increased levels of monomeric α-syn, the COS-7 cells with prefibrillar α-syn had lower levels of dopamine transporter on the cell surface, suggesting an impairment in protein trafficking and maturation in the medial Golgi (Figure 3).

72Another compromised mechanism is autophagy. Indeed, it has been found that, in human neuroblastoma SKNSH cells, the overexpression of α-syn inhibits RAb1A, which promotes mislocalization of the autophagy protein Atg9 in the TGN (an important feature for the autophagosome formation) (Figure 3). Furthermore, astrocytes derived from hESC exposed to oligomers from recombinant hWT α-syn showed accumulation of this form in the TGN. Interestingly, no GA fragmentation was observed. In addition, due to the fact that most of the autophagosome membranes come from ER and GA, studied various steps of autophagy pathway, like formation of conjugated LC3B, LC3BII/I ratio, and expression of p62. They observed that α-syn oligomers disrupted autophagosome/lysosome function, suggesting that the accumulation of α-syn aggregates in the TGN could impair the autophagosomal and the mitophagy pathways.

73Alpha-synuclein has also been related to function as an adaptor protein by linking to proteins involved in vesicular transport. In fact, in a toxicity study in yeast through overexpression of α-syn, it was reported that mutations in genes involved in vesicular traffic and in lipid metabolism increased lethality of α-syn overexpression, which led to suggest that these genes are the primary pathway that regulates toxicity of α-syn. Another evidence of the relation between α-syn and vesicular traffic was presented by using a model of S. cerevisiae with inducible expression of hWT α-syn or the p.A53T mutant. They observed accumulation of secretory and transport vesicles from ER to GA, and α-syn appeared to colocalize around the vesicles.

74In addition, a study of overexpression of hWT α-syn and the p.A53T mutant in yeast found an interruption in vesicular trafficking from the ER to the GA, although p.A53T induced faster blockage of vesicular trafficking than hWT α-syn (Figure 3). Interestingly, the time of the impairment in vesicular transport corresponded to the time of impairment of growth. Furthermore, the same study found that overexpression in genes promoting transport from the ER to the GA like Rab protein Ypt1p, the SNARE [soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor] protein, Ykt6b, the ubiquitin protease Ubp3p, etc., suppresses α-syn toxicity. Additionally, they found that Ypt1p was frequently localized in α-syn cytoplasmic inclusions. This suggested that the cytotoxic form of α-syn might associate with transport vesicles, as α-syn usually does with synaptic vesicles. In accordance with, they found that in yeast with expression of hWT α-syn, α-syn was colocalized with Rabs involved in retrograde endosome-Golgi transport (Ypt6p), intra-Golgi (from cis to trans Golgi side), and post-Golgi trafficking (Ypt31p, Ypt32p). However, they did not find direct interaction between Rabs and α-syn. However, they showed the ability of α-syn to induce accumulation and mislocalization of some Rab proteins.

75Besides, found a normal localization of Rab fusion proteins in WT yeast compared with expression of one copy of α-syn yeast, while yeast with expression of two copies of α-syn had an alteration in the localization of the Rabs Ypt1, Ypt31, Sec4, Ypt6, Vps21, and Ypt52, and was colocalized with α-syn. Also, it was found that α-syn did not alter vesicle buds and their target to cell periphery; however, α-syn avoided the docking and/or the fusion of vesicles. Moreover, due to evidence that Rab1 and Ypt1 rescue dopaminergic neurons of α-syn toxicity in Drosophila, C. elegans, and primary cultures of rat midbrain neurons, it has been suggested that trafficking impairment starts at the ER and proceeds to the GA.

76It has been observed that, for toxic inclusions, formed by α-syn-positive vesicular clusters that colocalize with protein markers of several trafficking routes, a great proportion of this protein is phosphorylated. Interestingly, by blocking α-syn phosphorylation in yeast, there is an augmentation in the toxicity observed that affect cellular trafficking. In addition, found that the accumulation of hWT α-syn induced an autophagic response. In contrast, the mutant phosphorylated form (pS129-α-syn) showed paired activity in the same pathway, reducing aggregation levels. Interestingly, they also observed that the expression of two unphosphorylated mutants, S129A-α-syn and S129E-α-syn, promoted the formation of α-syn toxic inclusions and diminished the capacity of the cell to eliminate them. Therefore, blocking α-syn phosphorylation promotes cytotoxicity in yeasts.

77Finally, a study carried out with rat kidney epithelial (NRK) cells with different ranges of expression of hWT α-syn showed that at low expression levels, it was associated with minimal inhibition of transport from the ER to the GA, while high levels of expression increased inhibition proportionally. Meanwhile, in cells with expression of the p.A53T α-syn mutant, the inhibition was almost complete in low expression. In addition, in rat dopaminergic neuroendocrine (PC12) cells, the expression of the p.A53T α-syn mutant showed lower transport inhibition (around 30%) compared to NRK cells (around 50%), maybe due to the existence of a protection mechanism or factor against α-syn. The same group found that the p.A53T α-syn mutant caused antagonisms of α-syn and SNAREs, which are part of the trafficking machinery from the ER to the GA and could be the cause of delay in transport. Also, α-syn inhibits the pre-Golgi coat protein complex II vesicle docking and fusion in vitro. An extensive review about α-syn and membrane trafficking can be found in.

78The endolysosomal system regulates vesicle traffic and it is involved in proteostasis. Several proteins seem to have relevance in α-syn accumulation in the endolysosomal system. One of them is the endosome and lysosome transmembrane protein ATP13A2, which suffers a mutation known as Dup22 (a duplication of 22 pair bases), resulting in a truncated protein presenting 6 of 10 transmembrane domains. With this truncated protein, the aggregation of α-syn and ER stress is promoted in H4 neuroglioma cells, while the depletion of ATP13A2, using a short hairpin RNA, promotes the aggregation of α-syn, leading to cell death by decreased lysosomal activity in primary cortical neurons.

79Another molecule that may promote α-syn accumulation is vacuolar protein sorting-associated protein 35 (VPS35). VPS35 is a component of the retromer complex involved in the transport of the endosome to the TGN, the membrane protein retrieval, the endosomal recycling, and a rare form of autosomal-dominant parkinsonism. It has been shown in mice with a VPS35 deletion that oligomeric and phosphorylated α-syn is increased. Furthermore, it was found that intracellular α-syn is increased in tyrosine hydroxylase (TH, the rate-limiting enzyme of catecholamine biosynthesis) positive neurons of the SN pars compacta. In the same model, the expression of Lamp2a, a receptor of chaperone-mediated autophagy (CMA), decreases, suggesting unpaired α-syn degradation via CMA, as a result of an alteration in lysosomes, due to a deregulation of Lamp2a. Moreover, in transgenic mice with α-syn overexpression, it was localized in the lysosomal lumen. In addition, an increase of Lamp2a was observed in nigral dopaminergic neurons also with α-syn overexpression, which indicates that the CMA is induced to clear the excess of α-syn.

80To help in the TGN tasks, the GGAs proteins (Golgi-localized, gamma adaptin ear-containing, ARF-binding), a group of coat proteins, are recruited from the cytosol onto the TGN, where they have the function of protein transport to the endosome/lysosome system. In this sense, when N2A and HEK293 cells overexpress GGAs proteins, they showed an increase in oligomeric α-syn secreted to the medium. The role of GGAs in the secretion of oligomeric α-syn makes it interesting for the study of propagation of α-syn, considering the conjecture of PD as a prion disease (see section “Introduction”).

81Interestingly, the overexpression of α-syn in COS-7 and SH-SY5Y cells showed that just the oligomers of α-syn and not the fibrillary form can be degraded by the lysosomal system, while the inhibition of the lysosome causes the accumulation of the aggregates of α-syn. Recently, in the experiment of, the hiPSC-derived midbrain dopaminergic neurons showed a basal proteolysis rate using leupeptin, which is a protease inhibitor of enzymes in lysosomes. Nonetheless, the overexpression of hWT α-syn by a lentivirus produced a lysosomal dysfunction, which declined the proteolysis rate showing increased lysosomal mass and a reduction of the hydrolase activity. Lysosomal enzymes such as cathepsin B and hexosaminidase showed a significant reduction of their activity; this was also observed in non-lysosomal enzymes like β-galactosidase in the GA when hWT α-syn was overexpressed. The reduction of this activity was due to defects in vesicular traffic. The results from the experiments described were confirmed by measuring the hydrolase mature:immature rate of hexosamine A and B. Both proteins acted in the lysosome and the result revealed enzyme accumulation in ER-GA vesicles, implying that the accumulation of α-syn was altering the lysosomal degradation by interrupting vesicular traffic between the ER and the GA.

82In summary, dysfunction in vesicular traffic and impairments in membranous organelles, such as the synthesis of proteins and lipids in the ER, post-translational modifications, and sorting and delivery of vesicles by the GA and the endolysosomal system, can be a result of the accumulation of α-syn. Concurrently, the accumulation of α-syn leads to cellular impairment and damage of the homeostatic balance. Therefore, it is not clear if α-syn accumulation is the first step of cytotoxicity or if it is a consequence of impairments in these organelles.

83The last two decades have been critical in gaining knowledge about α-syn function, in part due to the development of in vitro and in vivo models that, despite not replicating PD entirely, resemble pathological processes in humans (e.g., transgenic mouse models, human stem cell modeling), especially in the context of PD and other synucleinopathies. α-syn has a fundamental role, given its multiple interactions in several subcellular compartments and through the multiple conformations (monomer, oligomer, and fibril conformation), as well as its several post-translational modifications, which generate a spectrum of adverse effects for the cell that can exacerbate and finally culminate in cell death. At the same time, some physiological roles of α-syn have been also enlightened, especially in bioenergetic control, as well as vesicle transport from the GA to the ER, which outlines the importance of this protein for its cellular function. Despite these advances, the role of α-syn in early events of the disease process remains to be determined, including developmental and differentiation events of dopaminergic neurons. This will uncover early biomarkers and even advance therapeutic strategies to modify the natural history of the disease.

84LB-C and RA-S: analysis of mitochondrial-alpha synuclein interactions and overview of prionic diseases. RR-A and IM-M: implications of synuclein with nucleus and DNA. MR-H: description of the interactions of alpha-synuclein with soluble proteins and endolysosomal system. AB-O: review of alpha synuclein and organelles involved in vesicular trafficking. BS: analysis, writing modifications, and feedback through all the text. MG-C: supervision of research program, writing, analysis and interpretation throughout all the text, and editing of the manuscript.

85The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.