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Does the human placenta express the canonical cell entry mediators for SARS-CoV-2?

Roger Pique-Regi, Roberto Romero, Adi L Tarca and 6 more

eLife · 2020 · CC BY

doi:10.1101/2020.05.18.101485

Abstract

The pandemic of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected more than 10 million people, including pregnant women. To date, no consistent evidence for the vertical transmission of SARS-CoV-2 exists. The novel coronavirus canonically utilizes the angiotensin-converting enzyme 2 (ACE2) receptor and the serine protease TMPRSS2 for cell entry. Herein, building upon our previous single-cell study (Pique-Regi et al., 2019), another study, and new single-cell/nuclei RNA-sequencing data, we investigated the expression of ACE2 and TMPRSS2 throughout pregnancy in the placenta as well as in third-trimester chorioamniotic membranes. We report that co-transcription of ACE2 and TMPRSS2 is negligible in the placenta, thus not a likely path of vertical transmission for SARS-CoV-2. By contrast, receptors for Zika virus and cytomegalovirus, which cause congenital infections, are highly expressed by placental cell types. These data show that the placenta minimally expresses the canonical cell-entry mediators for SARS-CoV-2.

Full text

From PubMed Central, U.S. National Library of Medicine, as current on Oct 19, 2026. Paragraph numbers are ours, so an answer can point at one.

Introduction

The placenta serves as the lungs, gut, kidneys, and liver of the fetus. This fetal organ also has major biological actions that modulate maternal physiology and, importantly, together with the extraplacental chorioamniotic membranes, shield the fetus against microbes from hematogenous dissemination and from invading the amniotic cavity. Indeed, most pathogens that cause hematogenous infections in the mother cannot reach the fetus, which is largely due to the potent protective mechanisms provided by placental cells (i.e. trophoblast cells: syncytiotrophoblasts and cytotrophoblasts). Yet, some of these pathogens such as Toxoplasma gondii, Rubella virus, herpesvirus (HSV), cytomegalovirus (CMV), and Zika virus (ZIKV), among others, are capable of crossing the placenta and infecting the fetus, thus causing congenital disease.

In December 2019, a local outbreak of pneumonia caused by a novel coronavirus—severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)—was reported in Wuhan (Hubei, China). After exposure to SARS-COV-2, susceptible individuals can develop coronavirus disease 2019 (COVID-19) consisting of symptoms that may range from fever and cough to severe respiratory illness; in some cases, COVID-19 is life-threatening. Since the onset of the outbreak, more than 10 million COVID-19 cases have been confirmed, accounting for more than 500,000 deaths. This pandemic has now spread throughout the entire world with recent epicenters in Europe (Italy and Spain) and the United States. By April 2019, the states of New York and Michigan were the most severely affected, given that the metropolitan areas of New York City and Detroit possess large populations subject to health disparities that include limited access to health care, chronic exposure to pollution, and pre-existing cardiovascular conditions.

Pregnant women and their fetuses represent a potential high-risk population in light of the COVID-19 outbreak given that viral infections such as influenza, varicella, Ebola, and measles show increased severity in this physiological state. Other coronaviruses, such as SARS-CoV-1 and MERS-CoV, have severe effects in both the mother and the fetus, but vertical transmission has not been proven, albeit these studies included very few cases. By contrast with the above-mentioned viral infections, only ~15% of pregnant women test positive for SARS-CoV-2 and a small fraction of them are symptomatic, most of whom experience only a mild illness. Consequently, the clinical characteristics of pregnant women with COVID-19 appear similar to those of non-pregnant adults. Yet, recent reports have shown that severe COVID-19 can lead to maternal death. However, thus far, no conclusive evidence of vertical transmission has been generated. Consistently, infants born to mothers with COVID-19 test negative for SARS-CoV-2, do not develop serious clinical symptoms (e.g., fever, cough, diarrhea, or abnormal radiologic or hematologic evidence), and are promptly discharged from the hospital. Nevertheless, new evidence has emerged suggesting that the fetus can respond to SARS-CoV-2 infection.

Case reports have shown that a small fraction of neonates born to women with COVID-19 tested positive for the virus at 1–4 days of life, yet these neonates subsequently tested negative on day 6-7. In addition, serological studies revealed that a few neonates born to mothers with COVID-19 had increased concentrations of SARS-CoV-2 immunoglobulin (Ig)M as well as IgG. The elevated concentrations of IgG are likely due to the passive transfer of this immunoglobulin from the mother to the fetus across the placenta. However, the increased levels of IgM suggest that the fetus was infected with SARS-CoV-2 given that this immunoglobulin cannot cross the placenta as a result of its large molecular weight. Nonetheless, all neonates included in the above-mentioned studies tested negative for the virus and did not present any symptoms.

More recently, two case reports indicated that SARS-CoV-2 RNA has been detected in the amniotic fluid and placental tissues. In the first case report, the viral RNA was detected in amniotic fluid from a woman who was severely affected and died of COVID-19. The premature neonate tested negative for SARS-CoV-2 after delivery but 24 hr later tested positive. In the second case report, the viral RNA was detected in the placenta and umbilical cord from a woman with severe pre-eclampsia, placental abruption, and other complications, yet none of the fetal tissues tested positive. Therefore, whether SARS-CoV-2 can reach the fetus by crossing the placenta is still unclear.

Results and discussion

Cell entry and the spread of SARS-CoV-2 are widely thought to depend on the angiotensin-converting enzyme 2 (ACE2) receptor and the serine protease TMPRSS2. In the study herein, we investigated whether the receptors responsible for SARS-CoV-2 infection are expressed in the human placenta (including the decidual tissues) throughout the three trimesters of pregnancy by using publicly available single-cell RNA-sequencing (scRNA-seq) data together with newly generated data (Supplementary file 1).

Strikingly, we found that very few cells co-express ACE2 and TMPRSS2 (Figure 1A and B). Using a very permissive threshold of expression of one transcript per cell, only four cells with co-expression were detected in any of the three trimesters, resulting in an estimated <1/10,000 cells. Our first-trimester data are in agreement with a prior report showing minimal expression of ACE2 at the human maternal-fetal interface; however, the same dataset was recently used to report the opposite. Nonetheless, the co-expression of ACE2 and TMPRSS2 was not examined by either study, and it is important to consider log-transformation and data analysis issues for low-expressed genes, focusing on the fraction of cells expressing the transcripts. We also evaluated the expression of SARS-CoV-2 receptors in the chorioamniotic membranes (also known as the extraplacental membranes) in the third trimester; these tissues may also serve as a point of entry for microbial invasion of the amniotic cavity and potentially the fetus. Again, co-expression of ACE2 and TMPRSS2 was minimally detected in the chorioamniotic membranes (Figure 1A and B).

A challenge in scRNA-seq studies is generating high-quality, single-cell suspensions containing both rare and difficult-to-dissociate (e.g., multinucleated cells) cell types. This is likely the reason why the reported scRNA-seq studies of the human placenta contain a low fraction of syncytiotrophoblast cells [STB, multinucleated cells forming the outermost fetal component of the placenta in direct contact with the maternal circulation (i.e., intervillous space)]. Therefore, we considered whether the expression of ACE2 and TMPRSS2 was minimally observed in the placental cell types as a result of the reduced fraction of STB cells (i.e., dissociation bias). To address this possibility, we prepared single-nucleus suspensions of the placental tissues (including the decidua basalis) and performed single-nuclear RNAseq (snRNA-seq), which reduces the dissociation bias against large cells. An important advantage of snRNA-seq is its compatibility with biobank-frozen samples; therefore, we pooled 32 placental villi/decidua samples collected in the third trimester (Supplementary file 2). This represents the first snRNA-seq study of the placental tissues. As expected, a larger fraction of STB cells/nuclei was observed using snRNA-seq compared to scRNA-seq (Figure 1A and Figure 1—figure supplement 1). Consistent with the scRNAseq analyses, the snRNAseq data demonstrated that co-expression of ACE2 and TMPRSS2 is unlikely in the placental tissues (Figure 1B). A limitation of snRNA-seq is that it has a higher background compared to scRNAseq and could capture ACE2 and TMPRSS2 transcripts from other cell types, but this should not affect the analyses reported herein because co-expression is not observed.

Finally, we explored the expression of ACE2 and TMPRSS2 in third-trimester placental tissues by mining two microarray datasets that we have previously reported. These analyses of bulk gene expression data revealed that, while ACE2 was detected above background in most of the samples, TMPRSS2 was largely undetected (Supplementary file 3). Collectively, these results consistently indicate that the human placental tissues negligibly co-express ACE2 and TMPRSS2. This reduced expression contrasts with the high expression of ACE2 and TMPRSS2 in nasal goblet and ciliated cells within the human airways, lungs, and gastrointestinal tract, which are targeted during COVID-19. Therefore, our results suggest that vertical transmission of SARS-CoV-2 is unlikely to occur unless facilitated by other concomitant pathological conditions resulting in a breach of the maternal-fetal crosstalk.

There is a possibility, however, that SARS-CoV-2 could infect the human placenta by using alternate entry routes while interacting with other proteins. The expression of additional SARS-CoV-2-related receptors or proteins in the human placenta is shown in Figure 2, CoV-Alt; however, further research is required to test their participation in the pathogenesis of COVID-19. For example, in vitro studies suggest that BSG (Basigin, also called CD147 or EMMPRIN, transmembrane glycoprotein belonging to the immunoglobulin superfamily) provides an alternate entry for SARS-CoV-2 when ACE2 and TMPRSS2 are not expressed. We found that the placenta and chorioamniotic membranes expressed high levels of BSG throughout pregnancy (Figure 2, CoV-Alt), yet this transcript is also widely expressed in all human tissues and cell types (Figure 2—figure supplement 1). Therefore, it is unlikely that this protein alone is a sufficient requirement for SARS-CoV-2 viral entry, and other proteins may be required to explain the cell type primarily affected by COVID-19. Moreover, cathepsin L (CSTL) and FURIN may also function as proteases priming the SARS-CoV-2 S protein. We found that these proteases are highly expressed by the placental tissues throughout gestation (Figure 2, CoV-Alt). Nevertheless, these proteases may not provide sufficient levels of priming by themselves when tested with SARS-CoV-1, yet this has not been verified for SARS-CoV-2. Given that the placental tissues are enriched in maternal and fetal macrophages, and that a subset of these immune cells expressing sialoadhesin (SIGLEC1, also known as CD169) can contribute to viral spread during SARS-CoV-2 infection, we also investigated the expression of SIGLEC1 in this study. As expected, SIGLEC1 was expressed by macrophages in the placenta and chorioamniotic membranes and, to a lesser extent, in T cells (Figure 2, CoV-Alt). However, even if the virus could infect the placental/decidual macrophages expressing SIGLEC1, this is not sufficient for viral spreading. The expression of ADAM17 was also investigated in the placental tissues as this metalloproteinase competes with TMPRSS2 in ACE2 processing. The placenta and chorioamniotic membranes highly expressed ADAM17 (Figure 2—figure supplement 2); however, only cleavage by TMPRSS2 results in augmented SARS-S-driven cell entry. While these CoV-Alt molecules may be used for SARS-CoV-2 infection, they are likely to be less efficient than ACE2 and TMPRSS2, which are already targeted for antiviral interventions; yet, new candidate host:viral interacting proteins and possible drugs are being investigated.

Given that the main mediators for cell entry of SARS-CoV-2 were minimally expressed by the human placenta, we also investigated whether the receptors for congenital viruses such as CMV and ZIKV, which are known to infect and cross the placenta, were detectable using our pipeline. Known receptors for CMV include NRP2, PDFGRA, and CD46. Notably, all of these receptors were highly expressed in several placental cell types (Figure 2, CMV and Figure 2—figure supplement 2). Next, we investigated the expression of the AXL receptor for ZIKV as well as other related molecules such as CD209 and TYRO3. Consistent with vertical transmission, AXL, the preferred receptor for ZIKV, was highly expressed by the cells of the human placenta and chorioamniotic membranes throughout gestation (Figure 2, ZIKV). The expression of CD209 was mainly found in the maternal and fetal macrophage subsets, as expected. Yet, the expression of TYRO3 was low (Figure 2—figure supplement 2), consistent with the view that TAM receptors are not essential for ZIKV infection. The expression of other viral receptors involved in congenital disease was also documented in the placental tissues (Figure 2—figure supplement 2).

Conclusion

In conclusion, the single-cell transcriptomic analysis presented herein provides evidence that SARS-CoV-2 is unlikely to infect the placenta and fetus since its canonical receptor and protease, ACE2 and TMPRSS2, are only minimally expressed by the human placenta throughout pregnancy. In addition, we showed that the SARS-CoV-2 receptors are not expressed by the chorioamniotic membranes in the third trimester. However, viral receptors utilized by CMV, ZIKV, and others are highly expressed by the human placental tissues. While transcript levels do not always correlate with protein expression, our data indicate a low likelihood of placental infection and vertical transmission of SARS-CoV-2. However, it is still possible that the expression of these proteins is much higher in individuals with pregnancy complications related to the renin-angiotensin-aldosterone system (RAAS), which can alter the expression of ACE2. The cellular receptors and mechanisms that could be exploited by SARS-CoV-2 are still under investigation, yet single-cell atlases can help to identify cell types with a similar transcriptional profile to those that are known to participate in COVID-19.

Data availability

Placental and decidual scRNA-seq data from first-trimester samples were downloaded through ArrayExpress (E-MTAB-6701). Data for third-trimester samples previously collected by our group are available through NIH dbGAP (accession number phs001886.v2.p1), and newly generated second-trimester scRNA-seq and third-trimester snRNA-seq data are being deposited into the same repository (Supplementary file 1). All software and R packages used herein are detailed in the ‘scRNA-seq and snRNA-seq data analysis.’ Scripts detailing the analyses are also available at https://github.com/piquelab/sclabor (; copy archived at https://github.com/elifesciences-publications/sclabor).

Human subjects

Placental tissues were obtained immediately after a clinically indicated delivery from (i) a patient diagnosed with placenta accreta at 18 weeks of gestation and (ii) 32 patients spanning different conditions in the third trimester (Supplementary file 2). A sample of the basal plate of the placenta including the decidua basalis and placental villi tissue was (i) dissociated as previously described for scRNA-seq or (ii) preserved in RNAlater and subsequently frozen for snRNA-seq. The collection and use of human materials for research purposes were approved by the Institutional Review Boards of the Wayne State University School of Medicine and NICHD. All participating women provided written informed consent prior to sample collection.

Single-cell preparation

Cells from the placental villi and basal plate were isolated by enzymatic digestion using previously described protocols with modifications. Briefly, placental tissues were homogenized using a gentleMACS Dissociator (Miltenyi Biotec, San Diego, CA) either in an enzyme cocktail from the Umbilical Cord Dissociation Kit (Miltenyi Biotec) or in collagenase A (Sigma Aldrich, St. Louis, MO). After digestion, homogenized tissues were washed with ice-cold 1X phosphate-buffered saline (PBS) and filtered through a cell strainer (Fisher Scientific, Durham, NC). Cell suspensions were then collected and centrifuged at 300 x g for 5 min. at 4°C. Red blood cells were lysed using a lysing buffer (Life Technologies, Grand Island, NY). Next, the cells were washed with ice-cold 1X PBS and resuspended in 1X PBS for cell counting using an automatic cell counter (Cellometer Auto 2000; Nexcelom Bioscience, Lawrence, MA). Lastly, dead cells were removed from the cell suspensions using the Dead Cell Removal Kit (Miltenyi Biotec), and cells were counted again to determine final viable cell numbers.

Single-cell library preparation using the 10x genomics platform

Viable cells were utilized for single-cell RNAseq library construction using the Chromium Controller and Chromium Single Cell 3' Version 3 Kit (10x Genomics, Pleasanton, CA), following the manufacturer’s instructions. Briefly, viable cell suspensions were loaded into the Chromium Controller to generate gel beads in emulsion (GEM), with each GEM containing a single cell as well as barcoded oligonucleotides. Next, the GEMs were placed in the Veriti 96-well Thermal Cycler (Thermo Fisher Scientific, Wilmington, DE) and reverse transcription was performed in each GEM (GEM-RT). After the reaction, the complementary (c)DNA was cleaned by using Silane DynaBeads (Thermo Fisher Scientific) and the SPRIselect Reagent Kit (Beckman Coulter, Indianapolis, IN). Next, the cDNA was amplified using the Veriti 96-well Thermal Cycler and cleaned using the SPRIselect Reagent Kit. Indexed sequencing libraries were then constructed using the Chromium Single Cell 3' Version 3 Kit, following the manufacturer’s instructions.

cDNA was fragmented, end-repaired, and A-tailed using the Chromium Single Cell 3' Version 3 Kit, following the manufacturer’s instructions. Next, adaptor ligation was performed using the Chromium Single Cell 3' Version 3 Kit, followed by post-ligation clean-up using the SPRIselect Reagent Kit to obtain the final library constructs, which were then amplified using PCR. After performing a post-sample index double-sided size selection using the SPRIselect Reagent Kit, the quality and quantity of the DNA were analyzed using the Agilent Bioanalyzer High Sensitivity Chip (Agilent Technologies, Wilmington, DE). The Kapa DNA Quantification Kit for Illumina platforms (Kapa Biosystems, Wilmington, MA) was used to quantify the DNA libraries, following the manufacturer's instructions.

Single-nuclei sample preparation

We developed a new protocol to isolate nuclei from frozen placenta samples, based on DroNc-seq and an early version of the protocol developed by the Martelotto lab (https://www.protocols.io/view/frankenstein-protocol-for-nuclei-isolation-from-f-3eqgjdw). For each placenta sample, 1 mm frozen placenta biopsy punches were collected and immediately lysed with ice-cold lysis buffer (10 mM Tris-HCl, pH 7.5, 10 mM NaCl, 3 mM MgCl2, 2% BSA, 0.2 U/µl ROCHE Protector RNase Inhibitor, and 0.1% IGEPAL-630) for 5 min. During incubation the samples were gently mixed by swirling the tube twice and collected by centrifugation at 500 x g for 5 min at 4°C. The process was repeated twice for a total of 3 cycles of lysis (5 min long each). Next, the pellets were washed with ice-cold nuclei suspension buffer (1X PBS containing 2% BSA and 0.2 U/µl ROCHE Protector RNase Inhibitor) and filtered through a 30 μm cell strainer (Fisher Scientific). Nuclei suspensions were then collected and centrifuged at 500 x g for 5 min at 4°C. Nuclei were counted using a Countess II FL (Thermo Fisher Scientific, Durham, NC). All samples exhibited 100% cell death with DAPI staining, indicative of complete cell lysis. Nuclei were then utilized for single-nuclei RNAseq library construction using the Chromium Controller and Chromium Single Cell 3' version 2 kit (10x Genomics), following the manufacturer’s instructions.

Sequencing

Libraries were sequenced on the Illumina NextSeq 500 in the Luca/Pique-Regi laboratory and in the CMMG Genomics Services Center (GSC). The Illumina 75 Cycle Sequencing Kit was used with 58 cycles for R2, 26 for R1, and 8 for I1.

scRNA-seq and snRNA-seq data analyses

Raw fastq files were downloaded from previously established resources (as detailed in ‘Data Availability’), and the new sequencing data were processed using Cell Ranger version 3.0.0 from 10X Genomics for de-multiplexing. The fastq files were then aligned using kallisto, and bustools summarized the cell/gene transcript counts in a matrix for each sample, using the ‘lamanno’ workflow for scRNA-seq and the ‘nucleus’ workflow for snRNA-seq. Each sample was then processed using DIEM to eliminate debris and empty droplets for both scRNA-seq and snRNA-seq. To avoid the loss of cells that may express viral receptors, we did not exclude cell doublets from the analyses included in this report, which should have negligible effects on the results and conclusions. All count data matrices were then normalized and combined using the ‘NormalizeData,’ ‘FindVariableFeatures,’ and ‘ScaleData’ methods implemented in the Seurat package in R (Seurat version 3.1, R version 3.6.1) and. Afterward, the Seurat ‘RunPCA’ function was applied to obtain the first 50 principal components, and the different batches and locations were integrated and harmonized using the Harmony package in R. The top 30 harmony components were then processed using the Seurat ‘runUMAP’ function to embed and visualize the cells in a two-dimensional map via the Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) algorithm. To label the cells, the Seurat ‘FindTransferAnchors’ and ‘TransferData’ functions were used for each group of locations separately to assign a cell-type identity based on our previously labeled data as reference panel (as performed in ). Cell type abbreviations used are: STB, Syncytiotrophoblast; EVT, Extravillous trophoblast; CTB, cytotrophoblast; HSC, hematopoietic stem cell; npiCTB, non proliferative interstitial cytotrophoblast; LED, lymphoid endothelial decidual cell. Visualization of viral receptor gene expression was performed using the ggplot2 package in R with gene expression values scaled to transcripts per million (TPM) and to the proportion of cells expressing the transcript within a given cell type.

Bulk gene expression data analysis of ACE2 and TMPRSS2 in the placental tissues

Gene expression data for the study by was available from the www.ebi.ac.uk/microarray-as/ae/ database (entry ID: E-TABM-577), while data for the study by is available in our data repertoire. The mas5calls function from the affy package in Bioconductor was used to determine presence above background of each probeset corresponding to a given gene.

References 130

  1. 1.

    Aagaard KM, Lahon A, Suter MA, Arya RP, Seferovic MD, Vogt MB, Hu M, Stossi F, Mancini MA, Harris RA, Kahr M, Eppes C, Rac M, Belfort MA, Park CS, Lacorazza D, Rico-Hesse R. Primary human placental trophoblasts are permissive for zika virus (ZIKV) Replication. Scientific Reports. 2017;7:41389.

    Not in our library10.1038/srep41389

  2. 2.

    Adams Waldorf KM, Stencel-Baerenwald JE, Kapur RP, Studholme C, Boldenow E, Vornhagen J, Baldessari A, Dighe MK, Thiel J, Merillat S, Armistead B, Tisoncik-Go J, Green RR, Davis MA, Dewey EC, Fairgrieve MR, Gatenby JC, Richards T, Garden GA, Diamond MS, Juul SE, Grant RF, Kuller L, Shaw DW, Ogle J, Gough GM, Lee W, English C, Hevner RF, Dobyns WB, Gale M, Rajagopal L. Fetal brain lesions after subcutaneous inoculation of zika virus in a pregnant nonhuman primate. Nature Medicine. 2016;22:1256-1259.

    Not in our library10.1038/nm.4193

  3. 3.

    Adams Waldorf KM, Nelson BR, Stencel-Baerenwald JE, Studholme C, Kapur RP, Armistead B, Walker CL, Merillat S, Vornhagen J, Tisoncik-Go J, Baldessari A, Coleman M, Dighe MK, Shaw DWW, Roby JA, Santana-Ufret V, Boldenow E, Li J, Gao X, Davis MA, Swanstrom JA, Jensen K, Widman DG, Baric RS, Medwid JT, Hanley KA, Ogle J, Gough GM, Lee W, English C, Durning WM, Thiel J, Gatenby C, Dewey EC, Fairgrieve MR, Hodge RD, Grant RF, Kuller L, Dobyns WB, Hevner RF, Gale M, Rajagopal L. Congenital zika virus infection as a silent pathology with loss of neurogenic output in the fetal brain. Nature Medicine. 2018;24:368-374.

    Not in our library10.1038/nm.4485

  4. 4.

    Al-Haddad BJS, Oler E, Armistead B, Elsayed NA, Weinberger DR, Bernier R, Burd I, Kapur R, Jacobsson B, Wang C, Mysorekar I, Rajagopal L, Adams Waldorf KM. The fetal origins of mental illness. American Journal of Obstetrics and Gynecology. 2019;221:549-562.

    Not in our library10.1016/j.ajog.2019.06.013

  5. 5.

    Aldo P, You Y, Szigeti K, Horvath TL, Lindenbach B, Mor G. HSV-2 enhances ZIKV infection of the placenta and induces apoptosis in first-trimester trophoblast cells. American Journal of Reproductive Immunology. 2016;76:348-357.

    Not in our library10.1111/aji.12578

  6. 6.

    Alexandre J, Cracowski J-L, Richard V, Bouhanick B. Renin-angiotensin-aldosterone system and COVID-19 infection. Annales d'Endocrinologie. 2020;81:63-67.

    Not in our library10.1016/j.ando.2020.04.005

  7. 7.
    Impact of Middle East Respiratory Syndrome coronavirus (MERS‐CoV) on pregnancy and perinatal outcome · 2016
    No notice
  8. 8.

    Alvarez M. Enhancing droplet-based single-nucleus RNA-seq resolution using the semi-supervised machine learning classifier DIEM. bioRxiv. 2019.

    Not in our library10.1101/786285

  9. 9.

    Alzamora MC, Paredes T, Caceres D, Webb CM, Valdez LM, La Rosa M. Severe COVID-19 during pregnancy and possible vertical transmission. American Journal of Perinatology. 2020;37:861-865.

    Not in our library10.1055/s-0040-1710050

  10. 10.

    Ander SE, Diamond MS, Coyne CB. Immune responses at the maternal-fetal interface. Science Immunology. 2019;4:eaat6114.

    Not in our library10.1126/sciimmunol.aat6114

  11. 11.

    Arechavaleta-Velasco F, Koi H, Strauss JF, Parry S. Viral infection of the trophoblast: time to take a serious look at its role in abnormal implantation and placentation? Journal of Reproductive Immunology. 2002;55:113-121.

    Not in our library10.1016/s0165-0378(01)00143-7

  12. 12.

    Aronoff DM, Correa H, Rogers LM, Arav‐Boger R, Alcendor DJ. Placental pericytes and cytomegalovirus infectivity: Implications for HCMV placental pathology and congenital disease. American Journal of Reproductive Immunology. 2017;78:12728.

    Not in our library10.1111/aji.12728

  13. 13.

    Arora N, Sadovsky Y, Dermody TS, Coyne CB. Microbial vertical transmission during human pregnancy. Cell Host & Microbe. 2017;21:561-567.

    Not in our library10.1016/j.chom.2017.04.007

  14. 14.

    Ashokka B, Loh MH, Tan CH, Su LL, Young BE, Lye DC, Biswas A, Illanes SE, Choolani M. Care of the pregnant woman with coronavirus disease 2019 in labor and delivery: anesthesia, emergency cesarean delivery, differential diagnosis in the acutely ill parturient, care of the newborn, and protection of the healthcare personnel. American Journal of Obstetrics and Gynecology. 2020;223:66-74.

    Not in our library10.1016/j.ajog.2020.04.005

  15. 15.

    Atmar RL, Englund JA, Hammill H. Complications of measles during pregnancy. Clinical Infectious Diseases. 1992;14:217-226.

    Not in our library10.1093/clinids/14.1.217

  16. 16.

    Becht E, McInnes L, Healy J, Dutertre C-A, Kwok IWH, Ng LG, Ginhoux F, Newell EW. Dimensionality reduction for visualizing single-cell data using UMAP. Nature Biotechnology. 2019;37:38-44.

    Not in our library10.1038/nbt.4314

  17. 17.

    Blanco-Melo D, Nilsson-Payant BE, Liu WC, Uhl S, Hoagland D, Møller R, Jordan TX, Oishi K, Panis M, Sachs D, Wang TT, Schwartz RE, Lim JK, Albrecht RA, tenOever BR. Imbalanced host response to SARS-CoV-2 drives development of COVID-19. Cell. 2020;181:1036-1045.

    Not in our library10.1016/j.cell.2020.04.026

  18. 18.

    Blitz MJ. Maternal mortality among women with COVID-19 admitted to the intensive care unit. American Journal of Obstetrics and Gynecology. 2020;9378:30636-0.

    Not in our library10.1016/j.ajog.2020.06.020

  19. 19.

    Booeshaghi AS, Pachter L. Log(x+1)* and log(1+x)*. bioRxiv. 2020.

    Not in our library10.1101/2020.05.19.100214

  20. 20.

    Bray NL, Pimentel H, Melsted P, Pachter L. Near-optimal probabilistic RNA-seq quantification. Nature Biotechnology. 2016;34:525-527.

    Not in our library10.1038/nbt.3519

  21. 21.

    Burton GJ, Jauniaux E. What is the placenta? American Journal of Obstetrics and Gynecology. 2015;213:S6.e1-S6.e4.

    Not in our library10.1016/j.ajog.2015.07.050

  22. 22.

    Cao B, Parnell LA, Diamond MS, Mysorekar IU. Inhibition of autophagy limits vertical transmission of zika virus in pregnant mice. Journal of Experimental Medicine. 2017;214:2303-2313.

    Not in our library10.1084/jem.20170957

  23. 23.

    Carbaugh DL, Baric RS, Lazear HM. Envelope protein glycosylation mediates zika virus pathogenesis. Journal of Virology. 2019;93:19.

    Not in our library10.1128/jvi.00113-19

  24. 24.

    Centers for Disease Control and Prevention. Centers for Disease Control and Prevention. 2020a.

    Not in our library

  25. 25.

    Centers for Disease Control and Prevention. Centers for Disease Control and Prevention. 2020b.

    Not in our library

  26. 26.

    Cervantes-Gonzalez M, Launay O. Pandemic influenza A (H1N1) in pregnant women: impact of early diagnosis and antiviral treatment. Expert Review of Anti-Infective Therapy. 2010;8:981-984.

    Not in our library10.1586/eri.10.83

  27. 27.

    Chen Y. The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Directly decimates human spleens and lymph nodes. medRxiv. 2020.

    Not in our library10.1101/2020.03.27.20045427v1

  28. 28.

    Chen L, Li Q, Zheng D, Jiang H, Wei Y, Zou L, Feng L, Xiong G, Sun G, Wang H, Zhao Y, Qiao J. Clinical characteristics of pregnant women with Covid-19 in Wuhan, China. New England Journal of Medicine. 2020a;382:e100.

    Not in our library10.1056/nejmc2009226

  29. 29.

    Chen H, Guo J, Wang C, Luo F, Yu X, Zhang W, Li J, Zhao D, Xu D, Gong Q, Liao J, Yang H, Hou W, Zhang Y. Clinical characteristics and intrauterine vertical transmission potential of COVID-19 infection in nine pregnant women: a retrospective review of medical records. The Lancet. 2020b;395:809-815.

    Not in our library10.1016/s0140-6736(20)30360-3

  30. 30.
    Infants Born to Mothers With a New Coronavirus (COVID-19) · 2020
    No notice
  31. 31.

    Christensen PE, Schmidt H, Bang HO, Amdersen V, Jordal B, Jensen O. Measles in Virgin Soil, Greenland 1951. Danish Medical Bulletin. 1954;1:2-6.

    Not in our library

  32. 32.

    COVID-19. COVID-19 Dashboard by the Center for Systems Science and Engineering (CSSE) at Johns Hopkins University (JHU). 2020.

    Not in our library

  33. 33.

    Coyne CB, Lazear HM. Zika virus - reigniting the TORCH. Nature Reviews Microbiology. 2016;14:707-715.

    Not in our library10.1038/nrmicro.2016.125

  34. 34.

    Dashraath P, Wong JLJ, Lim MXK, Lim LM, Li S, Biswas A, Choolani M, Mattar C, Su LL. Coronavirus disease 2019 (COVID-19) pandemic and pregnancy. American Journal of Obstetrics and Gynecology. 2020;222:521-531.

    Not in our library10.1016/j.ajog.2020.03.021

  35. 35.

    Della Gatta AN, Rizzo R, Pilu G, Simonazzi G. Coronavirus disease 2019 during pregnancy: a systematic review of reported cases. American Journal of Obstetrics and Gynecology. 2020;223:36-41.

    Not in our library10.1016/j.ajog.2020.04.013

  36. 36.

    Dong E, Du H, Gardner L. An interactive web-based dashboard to track COVID-19 in real time. The Lancet Infectious Diseases. 2020a;20:533-534.

    Not in our library10.1016/s1473-3099(20)30120-1

  37. 37.

    Dong L, Tian J, He S, Zhu C, Wang J, Liu C, Yang J. Possible vertical transmission of SARS-CoV-2 from an infected mother to her newborn. Jama. 2020b;323:1846-1848.

    Not in our library10.1001/jama.2020.4621

  38. 38.

    Dudley DM, Van Rompay KK, Coffey LL, Ardeshir A, Keesler RI, Bliss-Moreau E, Grigsby PL, Steinbach RJ, Hirsch AJ, MacAllister RP, Pecoraro HL, Colgin LM, Hodge T, Streblow DN, Tardif S, Patterson JL, Tamhankar M, Seferovic M, Aagaard KM, Martín CS, Chiu CY, Panganiban AT, Veazey RS, Wang X, Maness NJ, Gilbert MH, Bohm RP, Adams Waldorf KM, Gale M, Rajagopal L, Hotchkiss CE, Mohr EL, Capuano SV, Simmons HA, Mejia A, Friedrich TC, Golos TG, O'Connor DH. Miscarriage and stillbirth following maternal zika virus infection in nonhuman primates. Nature Medicine. 2018;24:1104-1107.

    Not in our library10.1038/s41591-018-0088-5

  39. 39.

    Esmonde TF, Herdman G, Anderson G. Chickenpox pneumonia: an association with pregnancy. Thorax. 1989;44:812-815.

    Not in our library10.1136/thx.44.10.812

  40. 40.

    Faure Bardon V, Peytavin G, Lê MP, Guilleminot T, Elefant E, Stirnemann J, Leruez-Ville M, Ville Y. Placental transfer of letermovir & maribavir in the ex vivo human Cotyledon perfusion model new perspectives for in utero treatment of congenital Cytomegalovirus infection. PLOS ONE. 2020;15:e0232140.

    Not in our library10.1371/journal.pone.0232140

  41. 41.

    Fitzgerald W, Gomez-Lopez N, Erez O, Romero R, Margolis L. Extracellular vesicles generated by placental tissues ex vivo: a transport system for immune mediators and growth factors. American Journal of Reproductive Immunology. 2018;80:e12860.

    Not in our library10.1111/aji.12860

  42. 42.

    Gautier L, Cope L, Bolstad BM, Irizarry RA. Affy--analysis of affymetrix GeneChip data at the probe level. Bioinformatics. 2004;20:307-315.

    Not in our library10.1093/bioinformatics/btg405

  43. 43.

    Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O’Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huettenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang X-P, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O’Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu D, Wang H-Y, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d’Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, García-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. 2020;30:2286.

    Not in our library10.1038/s41586-020-2286-9

  44. 44.

    Haake DA, Zakowski PC, Haake DL, Bryson YJ. Early treatment with acyclovir for varicella pneumonia in otherwise healthy adults: retrospective controlled study and review. Clinical Infectious Diseases. 1990;12:788-798.

    Not in our library10.1093/clinids/12.5.788

  45. 45.

    Habib N, Avraham-Davidi I, Basu A, Burks T, Shekhar K, Hofree M, Choudhury SR, Aguet F, Gelfand E, Ardlie K, Weitz DA, Rozenblatt-Rosen O, Zhang F, Regev A. Massively parallel single-nucleus RNA-seq with DroNc-seq. Nature Methods. 2017;14:955-958.

    Not in our library10.1038/nmeth.4407

  46. 46.
    Normalization and variance stabilization of single-cell RNA-seq data using regularized negative binomial regression · 2019
    No notice
  47. 47.

    Hantoushzadeh S, Shamshirsaz AA, Aleyasin A, Seferovic MD, Aski SK, Arian SE, Pooransari P, Ghotbizadeh F, Aalipour S, Soleimani Z, Naemi M, Molaei B, Ahangari R, Salehi M, Oskoei AD, Pirozan P, Darkhaneh RF, Laki MG, Farani AK, Atrak S, Miri MM, Kouchek M, Shojaei S, Hadavand F, Keikha F, Hosseini MS, Borna S, Ariana S, Shariat M, Fatemi A, Nouri B, Nekooghadam SM, Aagaard K. Maternal death due to COVID-19. American Journal of Obstetrics and Gynecology. 2020;223:109.

    Not in our library10.1016/j.ajog.2020.04.030

  48. 48.

    Hastings AK, Yockey LJ, Jagger BW, Hwang J, Uraki R, Gaitsch HF, Parnell LA, Cao B, Mysorekar IU, Rothlin CV, Fikrig E, Diamond MS, Iwasaki A. TAM receptors are not required for zika virus infection in mice. Cell Reports. 2017;19:558-568.

    Not in our library10.1016/j.celrep.2017.03.058

  49. 49.

    HCA Lung Biological Network, Sungnak W, Huang N, Bécavin C, Berg M, Queen R, Litvinukova M, Talavera-López C, Maatz H, Reichart D, Sampaziotis F, Worlock KB, Yoshida M, Barnes JL. SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes. Nature Medicine. 2020;26:681-687.

    Not in our library10.1038/s41591-020-0868-6

  50. 50.

    Herse F, Dechend R, Harsem NK, Wallukat G, Janke J, Qadri F, Hering L, Muller DN, Luft FC, Staff AC. Dysregulation of the circulating and tissue-based renin-angiotensin system in preeclampsia. Hypertension. 2007;49:604-611.

    Not in our library10.1161/01.hyp.0000257797.49289.71

  51. 51.

    Heurich A, Hofmann-Winkler H, Gierer S, Liepold T, Jahn O, Pöhlmann S. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. Journal of Virology. 2014;88:1293-1307.

    Not in our library10.1128/jvi.02202-13

  52. 52.

    Hoffmann M, Kleine-Weber H, Schroeder S, Krüger N, Herrler T, Erichsen S, Schiergens TS, Herrler G, Wu NH, Nitsche A, Müller MA, Drosten C, Pöhlmann S. SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor. Cell. 2020;181:271-280.

    Not in our library10.1016/j.cell.2020.02.052

  53. 53.

    Hosier H. SARS-CoV-2 infection of the placenta. The Journal of Clinical Investigation. 2020;23:JCI139569.

    Not in our library10.1172/jci139569

  54. 54.

    Iwata-Yoshikawa N, Okamura T, Shimizu Y, Hasegawa H, Takeda M, Nagata N. TMPRSS2 contributes to virus spread and immunopathology in the airways of murine models after coronavirus infection. Journal of Virology. 2019;93:e01815-18.

    Not in our library10.1128/jvi.01815-18

  55. 55.
    Emerging Infections and Pregnancy · 2006
    No notice
  56. 56.

    Jeong SY, Sung SI, Sung JH, Ahn SY, Kang ES, Chang YS, Park WS, Kim JH. MERS-CoV infection in a pregnant woman in Korea. Journal of Korean Medical Science. 2017;32:1717-1720.

    Not in our library10.3346/jkms.2017.32.10.1717

  57. 57.

    Kim MJ, Romero R, Kim CJ, Tarca AL, Chhauy S, LaJeunesse C, Lee DC, Draghici S, Gotsch F, Kusanovic JP, Hassan SS, Kim JS. Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease. The Journal of Immunology. 2009;182:3919-3927.

    Not in our library10.4049/jimmunol.0803834

  58. 58.

    Kim CJ, Romero R, Chaemsaithong P, Chaiyasit N, Yoon BH, Kim YM. Acute chorioamnionitis and funisitis: definition, pathologic features, and clinical significance. American Journal of Obstetrics and Gynecology. 2015;213:S29-S52.

    Not in our library10.1016/j.ajog.2015.08.040

  59. 59.

    Koi H, Zhang J, Makrigiannakis A, Getsios S, MacCalman CD, Strauss JF, Parry S. Syncytiotrophoblast is a barrier to maternal-fetal transmission of herpes simplex virus. Biology of Reproduction. 2002;67:1572-1579.

    Not in our library10.1095/biolreprod.102.004325

  60. 60.

    Korsunsky I, Millard N, Fan J, Slowikowski K, Zhang F, Wei K, Baglaenko Y, Brenner M, Loh PR, Raychaudhuri S. Fast, sensitive and accurate integration of single-cell data with harmony. Nature Methods. 2019;16:1289-1296.

    Not in our library10.1038/s41592-019-0619-0

  61. 61.

    Kwon JY, Romero R, Mor G. New insights into the relationship between viral infection and pregnancy complications. American Journal of Reproductive Immunology. 2014;71:387-390.

    Not in our library10.1111/aji.12243

  62. 62.

    Lamouroux A, Attie-Bitach T, Martinovic J, Leruez-Ville M, Ville Y. Evidence for and against vertical transmission for severe acute respiratory syndrome coronavirus 2. American Journal of Obstetrics and Gynecology. 2020;223:91-9191.

    Not in our library10.1016/j.ajog.2020.04.039

  63. 63.
    Lack of Vertical Transmission of Severe Acute Respiratory Syndrome Coronavirus 2, China · 2020
    No notice
  64. 64.

    Lindsay L, Jackson LA, Savitz DA, Weber DJ, Koch GG, Kong L, Guess HA. Community influenza activity and risk of acute influenza-like illness episodes among healthy unvaccinated pregnant and postpartum women. American Journal of Epidemiology. 2006;163:838-848.

    Not in our library10.1093/aje/kwj095

  65. 65.

    Liu Y, Chen H, Tang K, Guo Y. Clinical manifestations and outcome of SARS-CoV-2 infection during pregnancy. Journal of Infection. 2020;4:28.

    Not in our library10.1016/j.jinf.2020.02.028

  66. 66.

    Lokken EM. Clinical characteristics of 46 pregnant women with a SARS-CoV-2 infection in Washington state. American Journal of Obstetrics and Gynecology. 2020;19:31.

    Not in our library10.1016/j.ajog.2020.05.031

  67. 67.
    SARS‐CoV‐2 receptor ACE2 and TMPRSS2 are primarily expressed in bronchial transient secretory cells · 2020
    No notice
  68. 68.

    Maltepe E, Fisher SJ. Placenta: the forgotten organ. Annual Review of Cell and Developmental Biology. 2015;31:523-552.

    Not in our library10.1146/annurev-cellbio-100814-125620

  69. 69.

    Martinez-Martin N, Marcandalli J, Huang CS, Arthur CP, Perotti M, Foglierini M, Ho H, Dosey AM, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L, Ciferri C. An unbiased screen for human Cytomegalovirus identifies Neuropilin-2 as a central viral receptor. Cell. 2018;174:1158-1171.

    Not in our library10.1016/j.cell.2018.06.028

  70. 70.

    McInnes L, Healy J, Melville J. UMAP: uniform manifold approximation and projection for dimension reduction. arXiv. 2020.

    Not in our library

  71. 71.

    Melsted P. Modular and efficient pre-processing of single-cell RNA-seq. bioRxiv. 2019.

    Not in our library10.1101/673285

  72. 72.

    Mosby LG, Rasmussen SA, Jamieson DJ. 2009 pandemic influenza A (H1N1) in pregnancy: a systematic review of the literature. American Journal of Obstetrics and Gynecology. 2011;205:10-18.

    Not in our library10.1016/j.ajog.2010.12.033

  73. 73.

    Muehlenbachs A, de la Rosa Vázquez O, Bausch DG, Schafer IJ, Paddock CD, Nyakio JP, Lame P, Bergeron E, McCollum AM, Goldsmith CS, Bollweg BC, Prieto MA, Lushima RS, Ilunga BK, Nichol ST, Shieh WJ, Ströher U, Rollin PE, Zaki SR. Ebola virus disease in pregnancy: clinical, Histopathologic, and immunohistochemical findings. Journal of Infectious Diseases. 2017;215:64-69.

    Not in our library10.1093/infdis/jiw206

  74. 74.

    Muus C. Integrated analyses of single-cell atlases reveal age, gender, and smoking status associations with cell type-specific expression of mediators of SARS-CoV-2 viral entry and highlights inflammatory programs in putative target cells. bioRxiv. 2020.

    Not in our library10.1101/2020.04.19.049254c

  75. 75.

    Mysorekar IU. Zika virus takes a transplacental route to infect fetuses: insights from an animal model. Missouri Medicine. 2017;114:168-170.

    Not in our library

  76. 76.

    Nelson BR, Roby JA, Dobyns WB, Rajagopal L, Gale M, Adams Waldorf KM. Immune evasion strategies used by zika virus to infect the fetal eye and brain. Viral Immunology. 2020;33:22-37.

    Not in our library10.1089/vim.2019.0082

  77. 77.

    Neuzil KM, Reed GW, Mitchel EF, Simonsen L, Griffin MR. Impact of influenza on acute cardiopulmonary hospitalizations in pregnant women. American Journal of Epidemiology. 1998;148:1094-1102.

    Not in our library10.1093/oxfordjournals.aje.a009587

  78. 78.

    Ng WF, Wong SF, Lam A, Mak YF, Yao H, Lee KC, Chow KM, Yu WC, Ho LC. The placentas of patients with severe acute respiratory syndrome: a pathophysiological evaluation. Pathology. 2006;38:210-218.

    Not in our library10.1080/00313020600696280

  79. 79.

    Olgun NS. Viral infections in pregnancy: a focus on ebola virus. Current Pharmaceutical Design. 2018;24:993-998.

    Not in our library10.2174/1381612824666180130121946

  80. 80.

    Oliveira LG, Peron JPS. Viral receptors for flaviviruses: not only gatekeepers. Journal of Leukocyte Biology. 2019;106:695-701.

    Not in our library10.1002/jlb.mr1118-460r

  81. 81.

    Pandemic H1N1 Influenza in Pregnancy Working Group, Siston AM, Rasmussen SA, Honein MA, Fry AM, Seib K, Callaghan WM, Louie J, Doyle TJ, Crockett M, Lynfield R, Moore Z, Wiedeman C, Anand M, Tabony L, Nielsen CF, Waller K, Page S, Thompson JM, Avery C, Springs CB, Jones T, Williams JL, Newsome K, Finelli L, Jamieson DJ. Pandemic 2009 influenza A(H1N1) virus illness among pregnant women in the united states. JAMA. 2010;303:1517-1525.

    Not in our library10.1001/jama.2010.479

  82. 82.

    Park MD. Macrophages: a trojan horse in COVID-19? Nature Reviews Immunology. 2020;20:351.

    Not in our library10.1038/s41577-020-0317-2

  83. 83.

    Parry S, Holder J, Strauss JF. Mechanisms of trophoblast-virus interaction. Journal of Reproductive Immunology. 1997;37:25-34.

    Not in our library10.1016/s0165-0378(97)00071-5

  84. 84.

    Paryani SG, Arvin AM. Intrauterine infection with varicella-zoster virus after maternal varicella. New England Journal of Medicine. 1986;314:1542-1546.

    Not in our library10.1056/nejm198606123142403

  85. 85.

    Pazos M, Sperling RS, Moran TM, Kraus TA. The influence of pregnancy on systemic immunity. Immunologic Research. 2012;54:254-261.

    Not in our library10.1007/s12026-012-8303-9

  86. 86.

    Pereira L, Tabata T, Petitt M, Fang-Hoover J. Congenital Cytomegalovirus infection undermines early development and functions of the human placenta. Placenta. 2017;59:S8-S16.

    Not in our library10.1016/j.placenta.2017.04.020

  87. 87.

    Persaud M, Martinez-Lopez A, Buffone C, Porcelli SA, Diaz-Griffero F. Infection by zika viruses requires the transmembrane protein AXL, endocytosis and low pH. Virology. 2018;518:301-312.

    Not in our library10.1016/j.virol.2018.03.009

  88. 88.
    Single cell transcriptional signatures of the human placenta in term and preterm parturition · 2019
    No notice
  89. 89.

    Pique-Regi R. GitHub. 2020.

    Not in our library

  90. 90.

    Raj RS, Bonney EA, Phillippe M. Influenza, immune system, and pregnancy. Reproductive Sciences. 2014;21:1434-1451.

    Not in our library10.1177/1933719114537720

  91. 91.

    Rasmussen SA, Smulian JC, Lednicky JA, Wen TS, Jamieson DJ. Coronavirus disease 2019 (COVID-19) and pregnancy: what obstetricians need to know. American Journal of Obstetrics and Gynecology. 2020;222:415-426.

    Not in our library10.1016/j.ajog.2020.02.017

  92. 92.

    Richard AS, Shim BS, Kwon YC, Zhang R, Otsuka Y, Schmitt K, Berri F, Diamond MS, Choe H. AXL-dependent infection of human fetal endothelial cells distinguishes zika virus from other pathogenic flaviviruses. PNAS. 2017;114:2024-2029.

    Not in our library10.1073/pnas.1620558114

  93. 93.

    Sasaki K, Norwitz ER. Gonadotropin-releasing hormone/gonadotropin-releasing hormone receptor signaling in the placenta. Current Opinion in Endocrinology & Diabetes and Obesity. 2011;18:401-408.

    Not in our library10.1097/med.0b013e32834cd3b0

  94. 94.

    Shang J, Ye G, Shi K, Wan Y, Luo C, Aihara H, Geng Q, Auerbach A, Li F. Structural basis of receptor recognition by SARS-CoV-2. Nature. 2020;581:221-224.

    Not in our library10.1038/s41586-020-2179-y

  95. 95.

    Shirato K, Kanou K, Kawase M, Matsuyama S. Clinical isolates of human coronavirus 229E bypass the endosome for cell entry. Journal of Virology. 2017;91:16.

    Not in our library10.1128/jvi.01387-16

  96. 96.

    Shirato K, Kawase M, Matsuyama S. Wild-type human coronaviruses prefer cell-surface TMPRSS2 to endosomal cathepsins for cell entry. Virology. 2018;517:9-15.

    Not in our library10.1016/j.virol.2017.11.012

  97. 97.

    Siberry GK, Reddy UM, Mofenson LM. SARS-COV-2 Maternal-Child transmission: can it occur before delivery and how do we prove it? The Pediatric Infectious Disease Journal. 2020;10:2820.

    Not in our library10.1097/inf.0000000000002820

  98. 98.

    Stegmann BJ, Carey JC. TORCH infections toxoplasmosis, other (syphilis, varicella-zoster, parvovirus B19), Rubella, Cytomegalovirus (CMV), and Herpes infections. Current Women's Health Reports. 2002;2:253-258.

    Not in our library

  99. 99.

    Stein KR, Gardner TJ, Hernandez RE, Kraus TA, Duty JA, Ubarretxena-Belandia I, Moran TM, Tortorella D. CD46 facilitates entry and dissemination of human Cytomegalovirus. Nature Communications. 2019;10:2699.

    Not in our library10.1038/s41467-019-10587-1

  100. 100.

    Stower H. Lack of maternal–fetal SARS-CoV-2 transmission. Nature Medicine. 2020;26:312.

    Not in our library10.1038/s41591-020-0810-y

  101. 101.

    Stuart T, Butler A, Hoffman P, Hafemeister C, Papalexi E, Mauck WM, Hao Y, Stoeckius M, Smibert P, Satija R. Comprehensive integration of Single-Cell data. Cell. 2019;177:1888-1902.

    Not in our library10.1016/j.cell.2019.05.031

  102. 102.

    Sutton D, Fuchs K, D'Alton M, Goffman D. Universal screening for SARS-CoV-2 in women admitted for delivery. New England Journal of Medicine. 2020;382:2163-2164.

    Not in our library10.1056/nejmc2009316

  103. 103.

    Svensson J, Jenmalm MC, Matussek A, Geffers R, Berg G, Ernerudh J. Macrophages at the fetal-maternal interface express markers of alternative activation and are induced by M-CSF and IL-10. The Journal of Immunology. 2011;187:3671-3682.

    Not in our library10.4049/jimmunol.1100130

  104. 104.

    Swamy GK, Dotters-Katz SK. Safety and varicella outcomes after varicella zoster immune globulin administration in pregnancy. American Journal of Obstetrics and Gynecology. 2019;221:655-656.

    Not in our library10.1016/j.ajog.2019.07.003

  105. 105.

    Swieboda D, Johnson EL, Beaver J, Haddad L, Enninga EAL, Hathcock M, Cordes S, Jean V, Lane I, Skountzou I, Chakraborty R. Baby's First Macrophage: Temporal Regulation of Hofbauer Cell Phenotype Influences Ligand-Mediated Innate Immune Responses across Gestation. The Journal of Immunology. 2020;204:2380-2391.

    Not in our library10.4049/jimmunol.1901185

  106. 106.

    Taglauer ES, Wilkins-Haug L, Bianchi DW. Review: cell-free fetal DNA in the maternal circulation as an indication of placental health and disease. Placenta. 2014;35:S64-S68.

    Not in our library10.1016/j.placenta.2013.11.014

  107. 107.

    Tekbali A. Pregnant versus non-pregnant SARS-CoV-2 and COVID-19 hospital admissions: the first 4 weeks in New York. American Journal of Obstetrics and Gynecology. 2020;223:126-127.

    Not in our library10.1016/j.ajog.2020.04.012

  108. 108.

    Toft JH, Lian IA, Tarca AL, Erez O, Espinoza J, Eide IP, Bjørge L, Draghici S, Romero R, Austgulen R. Whole-genome microarray and targeted analysis of angiogenesis-regulating gene expression (ENG, FLT1, VEGF, PlGF) in placentas from pre-eclamptic and small-for-gestational-age pregnancies. The Journal of Maternal-Fetal & Neonatal Medicine. 2008;21:267-273.

    Not in our library10.1080/14767050801924118

  109. 109.

    Triebwasser JH, Harris RE, Bryant RE, Rhoades ER. Varicella pneumonia in adults report of seven cases and a review of literature. Medicine. 1967;46:409-423.

    Not in our library

  110. 110.

    Tsang JCH, Vong JSL, Ji L, Poon LCY, Jiang P, Lui KO, Ni YB, To KF, Cheng YKY, Chiu RWK, Lo YMD. Integrative single-cell and cell-free plasma RNA transcriptomics elucidates placental cellular dynamics. PNAS. 2017;114:E7786-E7795.

    Not in our library10.1073/pnas.1710470114

  111. 111.

    Ulrich H, Pillat MM. CD147 as a target for COVID-19 treatment: suggested effects of azithromycin and stem cell engagement. Stem Cell Reviews and Reports. 2020;20:1-17.

    Not in our library10.1007/s12015-020-09976-7

  112. 112.

    Valentine GC, Seferovic MD, Fowler SW, Major AM, Gorchakov R, Berry R, Swennes AG, Murray KO, Suter MA, Aagaard KM. Timing of gestational exposure to zika virus is associated with postnatal growth restriction in a murine model. American Journal of Obstetrics and Gynecology. 2018;219:403.

    Not in our library10.1016/j.ajog.2018.06.005

  113. 113.

    Vento-Tormo R, Efremova M, Botting RA, Turco MY, Vento-Tormo M, Meyer KB, Park JE, Stephenson E, Polański K, Goncalves A, Gardner L, Holmqvist S, Henriksson J, Zou A, Sharkey AM, Millar B, Innes B, Wood L, Wilbrey-Clark A, Payne RP, Ivarsson MA, Lisgo S, Filby A, Rowitch DH, Bulmer JN, Wright GJ, Stubbington MJT, Haniffa M, Moffett A, Teichmann SA. Single-cell reconstruction of the early maternal-fetal interface in humans. Nature. 2018;563:347-353.

    Not in our library10.1038/s41586-018-0698-6

  114. 114.

    Vintzileos WS. Screening all pregnant women admitted to labor and delivery for the virus responsible for coronavirus disease. American Journal of Obstetrics and Gynecology. 2020;2019:24.

    Not in our library10.1016/j.ajog.2020.04.024

  115. 115.

    Wadman M. How does coronavirus kill? Clinicians trace a ferocious rampage through the body, from brain to toes. 2020.

    Not in our library

  116. 116.

    Walker CL, Little ME, Roby JA, Armistead B, Gale M, Rajagopal L, Nelson BR, Ehinger N, Mason B, Nayeri U, Curry CL, Adams Waldorf KM. Zika virus and the nonmicrocephalic fetus: why we should still worry. American Journal of Obstetrics and Gynecology. 2019;220:45-56.

    Not in our library10.1016/j.ajog.2018.08.035

  117. 117.

    Wang Q, Zhang Y, Wu L, Niu S, Song C, Zhang Z, Lu G, Qiao C, Hu Y, Yuen KY, Wang Q, Zhou H, Yan J, Qi J. Structural and functional basis of SARS-CoV-2 entry by using human ACE2. Cell. 2020a;181:894-904.

    Not in our library10.1016/j.cell.2020.03.045

  118. 118.

    Wang A. Single nucleus multiomic profiling reveals Age-Dynamic regulation of host genes associated with SARS-CoV-2 infection. bioRxiv. 2020b.

    Not in our library10.1101/2020.04.12.037580

  119. 119.
    CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells · 2020
    No notice
  120. 120.

    Weber LeBrun EE, Moawad NS, Rosenberg EI, Morey TE, Davies L, Collins WO, Smulian JC. COVID-19 pandemic: staged management of surgical services for gynecology and obstetrics. American Journal of Obstetrics and Gynecology. 2020;223:85.

    Not in our library10.1016/j.ajog.2020.03.038

  121. 121.

    Wickham H. ggplot2. Wiley Interdisciplinary Reviews: Computational Statistics. 2011;3:180-185.

    Not in our library10.1002/wics.147

  122. 122.

    Wong SF, Chow KM, Leung TN, Ng WF, Ng TK, Shek CC, Ng PC, Lam PW, Ho LC, To WW, Lai ST, Yan WW, Tan PY. Pregnancy and perinatal outcomes of women with severe acute respiratory syndrome. American Journal of Obstetrics and Gynecology. 2004;191:292-297.

    Not in our library10.1016/j.ajog.2003.11.019

  123. 123.

    Wu H, Kirita Y, Donnelly EL, Humphreys BD. Advantages of Single-Nucleus over Single-Cell RNA sequencing of adult kidney: rare cell types and novel cell states revealed in fibrosis. Journal of the American Society of Nephrology. 2019;30:23-32.

    Not in our library10.1681/asn.2018090912

  124. 124.

    Xu Y, Plazyo O, Romero R, Hassan SS, Gomez-Lopez N. Isolation of leukocytes from the human Maternal-fetal interface. Journal of Visualized Experiments. 2015;99:e52863.

    Not in our library10.3791/52863

  125. 125.

    Yan J, Guo J, Fan C, Juan J, Yu X, Li J, Feng L, Li C, Chen H, Qiao Y, Lei D, Wang C, Xiong G, Xiao F, He W, Pang Q, Hu X, Wang S, Chen D, Zhang Y, Poon LC, Yang H. Coronavirus disease 2019 in pregnant women: a report based on 116 cases. American Journal of Obstetrics and Gynecology. 2020;223:e14.

    Not in our library10.1016/j.ajog.2020.04.014

  126. 126.

    Yu N, Li W, Kang Q, Xiong Z, Wang S, Lin X, Liu Y, Xiao J, Liu H, Deng D, Chen S, Zeng W, Feng L, Wu J. Clinical features and obstetric and neonatal outcomes of pregnant patients with COVID-19 in Wuhan, China: a retrospective, single-centre, descriptive study. The Lancet Infectious Diseases. 2020;20:559-564.

    Not in our library10.1016/s1473-3099(20)30176-6

  127. 127.

    Zamaniyan M. Preterm delivery in pregnant woman with critical COVID-19 pneumonia and vertical transmission. Prenatal Diagnosis. 2020;7:5713.

    Not in our library10.1002/pd.5713

  128. 128.

    Zeng L, Xia S, Yuan W, Yan K, Xiao F, Shao J, Zhou W. Neonatal Early-Onset infection with SARS-CoV-2 in 33 neonates born to mothers with COVID-19 in Wuhan, China. JAMA Pediatrics. 2020a;174:722-725.

    Not in our library10.1001/jamapediatrics.2020.0878

  129. 129.

    Zeng H, Xu C, Fan J, Tang Y, Deng Q, Zhang W, Long X. Antibodies in infants born to mothers with COVID-19 pneumonia. Jama. 2020b;323:1848-1849.

    Not in our library10.1001/jama.2020.4861

  130. 130.
    Single-cell RNA expression profiling of ACE2 and AXL in the human maternal–Fetal interface · 2020
    Not checked

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