Tracking the embryonic stem cell transition from ground state pluripotency

Abstract

Mouse embryonic stem (ES) cells are locked into self-renewal by shielding from inductive cues. Release from this ground state in minimal conditions offers a system for delineating developmental progression from naïve pluripotency. Here, we examine the initial transition process. The ES cell population behaves asynchronously. We therefore exploited a short-half-life Rex1::GFP reporter to isolate cells either side of exit from naïve status. Extinction of ES cell identity in single cells is acute. It occurs only after near-complete elimination of naïve pluripotency factors, but precedes appearance of lineage specification markers. Cells newly departed from the ES cell state display features of early post-implantation epiblast and are distinct from primed epiblast. They also exhibit a genome-wide increase in DNA methylation, intermediate between early and late epiblast. These findings are consistent with the proposition that naïve cells transition to a distinct formative phase of pluripotency preparatory to lineage priming.

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Cited by papers we hold 10

• Dissection of two routes to naïve pluripotency using different kinase inhibitors · 2021
  Not checked
• Membrane Tension Gates ERK-Mediated Regulation of Pluripotent Cell Fate · 2021
  No notice
• Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency · 2019
  No notice
• Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis · 2019
  No notice
• Capacitation of human naïve pluripotent stem cells for multi-lineage differentiation · 2019
  No notice
• Defined conditions for propagation and manipulation of mouse embryonic stem cells · 2019
  No notice
• Micropattern differentiation of mouse pluripotent stem cells recapitulates embryo regionalized cell fate patterning · 2018
  No notice
• Single cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development · 2018
  No notice
• Formative pluripotency: the executive phase in a developmental continuum · 2017
  No notice
• Epigenetic resetting of human pluripotency · 2017
  No notice

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