No retraction, correction or concern found when last checked on Oct 30, 2026.

A Complete Electron Microscopy Volume of the Brain of Adult Drosophila melanogaster

Zhihao Zheng, J. Scott Lauritzen, Eric Perlman and 20 more

Cell · 2018 · CC BY

doi:10.1016/j.cell.2018.06.019

Abstract

Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping of synaptic connectivity; however, the fly brain is too large for conventional EM. We developed a custom high-throughput EM platform and imaged the entire brain of an adult female fly at synaptic resolution. To validate the dataset, we traced brain-spanning circuitry involving the mushroom body (MB), which has been extensively studied for its role in learning. All inputs to Kenyon cells (KCs), the intrinsic neurons of the MB, were mapped, revealing a previously unknown cell type, postsynaptic partners of KC dendrites, and unexpected clustering of olfactory projection neurons. These reconstructions show that this freely available EM volume supports mapping of brain-spanning circuits, which will significantly accelerate Drosophila neuroscience.

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A connectome of a learning and memory center in the adult Drosophila brain · 2017
No notice
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The comprehensive connectome of a neural substrate for ‘ON’ motion detection in Drosophila · 2017
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Tanaka N.K., Endo K., Ito K. Organization of antennal lobe-associated neurons in adult Drosophila melanogaster brain. J. Comp. Neurol. 2012;520:4067-4130.
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Wiring variations that enable and constrain neural computation in a sensory microcircuit · 2017
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Enhanced FIB-SEM systems for large-volume 3D imaging · 2017
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Yagi R., Mabuchi Y., Mizunami M., Tanaka N.K. Convergence of multimodal sensory pathways to the mushroom body calyx in Drosophila melanogaster. Sci. Rep. 2016;6:29481.
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A Complete Developmental Sequence of a Drosophila Neuronal Lineage as Revealed by Twin-Spot MARCM · 2010
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Cited by papers we hold 33

Functional connectomics spanning multiple areas of mouse visual cortex · 2025
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Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster · 2024
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Neuronal wiring diagram of an adult brain · 2024
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Drosophila gustatory projections are segregated by taste modality and connectivity · 2022
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Automated Synapse Detection Method for Cerebellar Connectomics · 2022
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DMRT Transcription Factors in the Control of Nervous System Sexual Differentiation · 2022
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neuPrint: An open access tool for EM connectomics · 2022
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Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons · 2022
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Volume electron microscopy · 2022
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Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits · 2021
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Transcriptome Analysis of NPFR Neurons Reveals a Connection Between Proteome Diversity and Social Behavior · 2021
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Exploiting Multiple Timescales in Hierarchical Echo State Networks · 2021
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Information flow, cell types and stereotypy in a full olfactory connectome · 2021
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A connectome of the Drosophila central complex reveals network motifs suitable for flexible navigation and context-dependent action selection · 2021
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Multiple network properties overcome random connectivity to enable stereotypic sensory responses · 2020
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A connectome and analysis of the adult Drosophila central brain · 2020
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3D mesh processing using GAMer 2 to enable reaction-diffusion simulations in realistic cellular geometries. · 2020
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Complete Connectomic Reconstruction of Olfactory Projection Neurons in the Fly Brain · 2020
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An unbiased template of the Drosophila brain and ventral nerve cord · 2020
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and 13 more

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