DNA-PK is a DNA sensor for IRF-3-dependent innate immunity

1The innate immune response is mediated by the production of cytokines, including type I interferons (IFNs), and chemokines following the detection of pathogen-specific molecules by host cells. Detection of nucleic acids is crucial in triggering the innate immune to pathogens, particularly in response to viruses. Various double and single stranded RNA substrates are recognised directly by the DExD/H box RNA helicases retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated gene-5 (MDA-5) in the cytoplasm and by the endosomal toll-like receptors (TLRs). The engagement of such RNA receptors leads to the rapid transcription of genes encoding anti-viral proteins via the activation of transcription factors belonging to the interferon regulatory factor (IRF) and nuclear factor-kappa B (NF-κB) families. It has been recognised for some time that intracellular DNA can activate a similar IRF-3-dependent innate immune response and it is known that this signalling pathway depends on both the IRF-3-activating kinase, TANK-binding kinase 1 (TBK1) and the adaptor protein stimulator of IFN genes (STING—also known as MITA, ERIS and TMEM173). Only more recently, however, have some candidate receptors for these pathways been identified. DNA-dependent activator of IFN-regulatory factors (DAI), RNA polymerase III (RNA-Pol III), IFN inducible gene 16 and DDX41 have been described as cytoplasmic DNA sensors that activate IRF-3. Nonetheless, the in vivo relevance of these sensors remains unknown and the normal immune response to DNA stimulation of Dai−/− and Ips1−/− cells and sensing of plasmodium DNA independent of these receptors indicates other IRF-3-activating cytoplasmic DNA sensors exist. In addition, although TBK1 and STING are essential for activation of IRF-3 following DNA stimulation, the molecular details of this signalling pathway are poorly understood.

2The presence of unidentified DNA sensors in fibroblasts is especially pertinent to virus infection since these cells are often a primary target of virus infection in vivo. These cells should have sentinel innate immune receptors in place to detect the presence of foreign nucleic acid and respond by producing IFN, cytokines and chemokines to initiate the anti-viral state in the surrounding tissue as well as to attract immune cells to the site of infection. In this study we identify DNA-dependent protein kinase (DNA-PK) as a novel DNA sensor in fibroblasts where it is present at high levels enabling it to respond to incoming infection without the need for prior stimulation. DNA-PK is a heterotrimeric protein complex consisting of three proteins, Ku70, Ku80 (also known as Ku86) and the catalytic subunit DNA-PKcs (encoded by the xrcc6, xrcc5 and prkcd genes respectively). Ku70 and Ku80 themselves form a heterodimer and the absence of one subunit de-stabilises the expression of the other. Both the Ku heterodimer and DNA-PKcs can bind directly to DNA but, in the absence of Ku the affinity of DNA-PKcs for DNA is greatly reduced. DNA-PK has a well described role in the nucleus where it is necessary for non-homologous end joining (NHEJ) and so has a key role in repairing double-strand DNA breaks. DNA-PK has also been detected in the cytoplasm by immunofluorescence and cell fractionation, although prior to this study no function has been assigned to DNA-PK in this localisation. Here we found that, in the cytoplasm, DNA-PK signals via IRF-3 to activate an anti-microbial innate immune response to DNA mediated by the production of IFN, cytokines and chemokines. We show that DNA-PK co-localises with sites of viral DNA replication during VACV infection and the innate immune response to DNA and to infection with vaccinia virus (VACV) and herpes simplex virus (HSV-1) was impaired in both cells and mice which lack components of DNA-PK.

3To identify novel cytoplasmic DNA sensors, we transfected biotinylated dsDNA composed of a concatenated 45-bp oligonucleotide (called immunostimulatory DNA, ISD,) into human embryonic kidney (HEK) 293T cells and isolated DNA/protein complexes from the cytoplasm by affinity purification (Figure 1A). The three abundant proteins that bound specifically to DNA, and not to biotinylated lipoprotein pam-3-cys, were unequivocally identified as Ku70, Ku80 and DNA-PKcs (Figure 1A). We confirmed by subcellular fractionation that DNA-PK is present in the cytoplasm of resting cells (Figure 1B), as reported previously and that this cytoplasmic localisation was not due to nuclear contamination (Figure 1B). The DNA pull-down was reproduced from murine fibroblasts (Figure 1C), showing the cross-species conservation of this interaction. Additionally, in murine embryonic fibroblasts (MEFs) lacking Ku80 (Xrcc5−/−), the remaining DNA-PK components did not interact with cytoplasmic DNA under the conditions tested, whereas in Prkdc−/− MEFs, Ku80 was still recruited (Figure 1C). Therefore, the association between DNA-PKcs and cytoplasmic DNA is enhanced by Ku.

4In fibroblasts, the transcription of genes encoding chemokines and cytokines was induced in response to DNA in a length and dose-dependent manner, was sensitive to DNase treatment and required DNA to be transfected into the cell (Figure 2A and data not shown). These observations are consistent with other studies. Next we tested DNA from various sources, including vaccinia virus (VACV) and Escherichia coli, for its ability to bind DNA-PK in the cytoplasm and to stimulate Cxcl10 transcription. Cxcl10 is strongly induced by intracellular nucleic acids via the IRF-3- and NF-κB-binding sites in its promoter. Each of these different DNA species associated with DNA-PK in MEFs, and this association correlated with Cxcl10 induction (Figure 2B,C—black bars), implying DNA sequence-independence of this response. A similar correlation between DNA binding and Il6 induction was also observed (data not shown).

5Strikingly, in MEFs lacking DNA-PKcs (Prkdc−/−) there was a significant impairment in Cxcl10 transcription in response to double stranded concatenated ISD DNA (from now on referred to as DNA), viral and bacterial DNA and poly(dA:dT) (Figure 2C, white bars). In contrast, the response to the dsRNA analogue, poly(I:C), was similar in the presence or absence of DNA-PKcs indicating the signalling defect is specific to DNA (Figure 2C). Time-course experiments indicated that the defect in DNA stimulation of Prkdc−/− cells was consistent at all times tested (Figure 2D) and that these cells are not simply responding to DNA with different kinetics. The data presented in Figure 2C,D were carried out with MEFs derived from separate strains of Prkdc−/− mice, indicating that this phenotype was not confined to a single MEF line. As a control we also tested the response to LPS (Figure 2D, right panel) and found the Prkdc−/− cells responded like the wild type cells, with typically rapid kinetics, to this stimulus. In addition, the secretion of Cxcl10 and Ifnβ as well as the transcription of ifnb, il6, and the chemokines Ccl4 and Ccl5, was also consistently impaired in multiple preparations of passage 1 Prkdc−/− MEFs in response to DNA but not RNA (Figure 2E–G). It is notable that, in MEFs, the transcription of other type 1 IFNs, type III IFNs and anti-inflammatory cytokines such as Il4 and Il10 was not observed in response to DNA stimulation (data not shown). Defects in the production of cytokines in response to DNA were found in transformed MEFs lacking Ku80 (Xrcc5−/−) (Figure 2H) or Ku70 (Xrcc6−/−) (not shown) and the transcription of Ifnb in response to DNA was also impaired in primary adult murine skin fibroblasts lacking both Ku genes (Figure 2I) indicating that this phenotype requires both Ku and DNA-PKcs and is not restricted to embryonic cells. Finally, the re-expression of Ku80 in Xrcc5−/− cells restored their DNA-dependent production of Cxcl10 to wild-type levels (Figure 2J). Collectively, these data indicate that DNA-PK acts as a DNA sensor by binding foreign DNA in the cytoplasm and activating a host innate immune response.

6We delimitated the signalling pathway downstream of DNA-PK by using various MEFs lacking specific signalling components. In Irf3−/−cells the increase in transcription of Ifnb, Cxcl10, Il6 and Isg54 (an IRF-3-dependent gene) in response to DNA was lost (Figure 3A). Equally, the up-regulation of Il6 and Cxcl10 mRNA was lost in Tbk1−/− MEFs (Figure 3B) and primary Tmem173−/− MEFs (data not shown). Similar results have been reported previously and in a similar manner confirmed that cytoplasmic DNA sensing in fibroblasts is independent of TLRs, IPS-1 (and hence both dsRNA-sensing pathways and RNA-pol III-dependent DNA sensing), and DAI, because there was no defect in DNA-dependent cytokine production in Myd88−/−/Ticam1−/−, Mavs−/− or Zbp1−/− MEFs (data not shown).

7To establish whether DNA-PK acts upstream of IRF-3 activation, we monitored IRF-3 translocation in response to DNA and RNA stimulation. In Prkdc−/− MEFs IRF-3 translocation in response to DNA, but not to RNA, was abrogated (Figure 4A) and we obtained similar results in Xrcc5−/− MEFs (Figure 4B). In contrast, translocation into the nucleus of the p65 component of NF-κB, was unaffected by absence of the Prkdc gene (Figure 4C). To confirm the activity of IRF-3 and NF-κB at the transcriptional level we examined induction of the IFN-inducible gene Isg54, which is entirely dependent on IRF-3 activity following DNA stimulation (Figure 3A). We also assayed expression of Nfkbia, a known NF-κB-dependent gene that encodes the IκBα protein. Induction of Isg54, but not Nfkbia, was impaired in Prkdc−/− MEFs in response to DNA but not RNA transfection (Figure 4D). These data confirm the DNA-specific defect in IRF-3, but not NF-κB, activation in cells lacking DNA-PKcs and demonstrate that DNA-PK acts as a DNA sensor upstream of the IRF-3-dependent innate immune response. Our data also imply that the existence of an additional DNA sensing pathway in MEFs that is independent of DNA-PK and capable of activating NF-κB.

8Previously, DNA-PK was shown to interact directly with IRF-3. We confirmed this interaction by co-immunoprecipitation of both proteins (data not shown). In the study of DNA-PKcs was shown to phosphorylate IRF-3 at its N terminus, so enhancing its nuclear retention. To test whether phosphorylation of IRF-3 is necessary for the DNA-sensing function of DNA-PK, two approaches were taken. First, we tested PrkdcSCID MEFs, which express a kinase-dead form of the DNA-PKcs protein lacking its C terminal 83 amino-acids, for their ability to produce Ifnb, Cxcl10 and Il-6 in response to DNA or RNA. However, PrkdcSCID MEFs showed no defect in induction of Ifnb (Figure 5A), Il-6 (Figure 5B), and Cxcl10 (Figure 5C) in response to either DNA or RNA stimulation. Second, we used the DNA-PKcs-specific inhibitor NU7026 to inhibit DNA-PKcs kinase activity in wild type MEFs. Cells treated with NU7026 showed no impairment in production of Cxcl10 in response to DNA transfection (Figure 5D). Hence, the kinase activity of DNA-PKcs is not required for activation of IRF-3 in response to DNA.

9The finding that the kinase activity of DNA-PKcs was not necessary for DNA sensing prompted an investigation of other interactions with signalling components. To do this we developed an inducible system to study the DNA sensing signalling pathway. HEK293 cells do not activate IRF3 in response to DNA stimulation due to a defect in STING, but by introducing an inducible version of STING into these cells, and then inducing STING expression with doxycycline, the cells activate IRF-3 as shown by its phosphorylation (Figure 5E) and produce cytokines (not shown) in response to double stranded ISD DNA concatamers. Using this system, immunoprecipitation showed that prior to DNA stimulation, STING exists in a complex with Ku70 but this interaction is abrogated following transfection of DNA (Figure 5F). These data suggest that DNA-PK interacts with the STING-dependent signalling pathway and this changes upon DNA activation.

10We tested the relevance of this novel DNA-PK-dependent DNA sensing mechanism to virus infection using VACV strain modified virus Ankara (MVA). MVA activates innate immunity via TLR-dependent and independent pathways and, during infection, VACV DNA accumulates in cytoplasmic virus factories in association with many virus proteins. We reasoned that such large aggregates of foreign DNA, present in a cellular compartment where DNA does not normally reside, would present an excellent target for an innate immune DNA-sensing mechanism. By 6 hr post infection, both Ku80 and DNA-PKcs had accumulated in these viral factories (Figure 6A) together with the IRF-3-activating kinase TBK1 (Figure 6B, left panel), consistent with its role in this sensing pathway, although IRF-3 was mostly nuclear, reflecting its activation by virus infection (Figure 6B, right panel).

11In fibroblasts, MVA induces an IRF-3-dependent response (Figure 7A) which is independent of TLR signalling, RNA sensing (and hence RNA-pol III-dependent DNA sensing) and DAI (data not shown), indicating that viral genomic DNA is a major target for the host response to VACV infection. We found the production of Cxcl10 and Il-6 was strongly impaired in cells lacking Prkdc−/− or Xrcc5−/− during MVA infection (Figure 7B,C) whereas the response of these cells to infection by Newcastle disease virus (NDV, an RNA virus) remained intact (Figure 7B,C). Isg54, but not Nfkbia, transcription was impaired in response to MVA infection in Prkdc−/− MEFs, whilst the response of both genes to NDV was equivalent (Figure 7D), directly indicating that the DNA-PKcs-dependent activation of IRF-3 is important in the response to DNA virus infection. Additionally, in primary Prkdc−/− MEFs, there was greater MVA protein synthesis 4–8 hr post infection (Figure 7E), not only re-enforcing the function of DNA-PKcs in the anti-viral response but also showing the failure to respond to DNA in Prkdc−/− MEFs is not due to a failure to infect these cells or synthesise viral macromolecules. These data provide direct evidence that the innate immune response to infection by a DNA virus is regulated by DNA-PK and confirm the role of this complex in cytoplasmic DNA sensing in the context of infection.

12To confirm that DNA-PK-dependent DNA sensing contributes to the innate immune response in vivo, we transfected nucleic acids directly into the ear pinnae of mice and assayed the induction of innate immune transcriptional responses by qPCR analysis of extracted RNA 12 hr later. DNA transfection induced both Ifnb and Il6 and this was significantly reduced in Prkdc−/− mice. By contrast, both WT and Prkdc−/− mice exhibited equivalent responses to poly(I:C) (Figure 8A). Hence, DNA-PKcs plays a key role in potentiating the innate immune response to ectopic DNA in vivo. We next infected WT and Prkdc−/− mice with either the DNA virus MVA or with influenza virus (an RNA virus), and assayed expression of Ifnb and Il6 12 hr later. The absence of DNA-PK severely impaired induction of Ifnb and Il6 in response to MVA but not to influenza virus (Figure 8B). Similarly, Prkdc−/− mice exhibited severe impairment of Il6 induction in response to HSV-1 (Figure 8C): no Ifnb induction could be detected in this experiment. Overall these data confirm that the innate immune response to DNA viruses immediately after infection is significantly dependent upon DNA-PK, despite the presence of other DNA sensors reported hitherto.

13The identification of DNA-PK as a DNA sensor advances understanding of the innate immune response to infection and expands the current repertoire of DNA sensing mechanisms. In this study we show that the three proteins which constitute the DNA-PK complex; Ku70, Ku80 and DNA-PKcs, bind in significant amounts to DNA transfected into the cytoplasm of resting cells leading to an IRF-3-dependent innate immune response. Consistent with this, we found DNA-PK and TBK1 localised to sites of DNA replication during virus infection. Previous studies have ruled out a role for DNA-PK in the production of Ifnb in response to DNA in monocytic cells and HEK293 cells. However, although DNA-PK components are expressed at high levels in a wide range of tissues and cell types, they are absent in primary macrophages ( and data not shown), and we now show that in fibroblasts and in mice the innate immune response to DNA and DNA viruses is dependent on DNA-PKcs. The in vivo deficiency in DNA sensing in the absence of DNA-PKcs is observed despite the presumed presence of other DNA sensors. The observation that this defect is not extended to RNA, LPS or to RNA viruses shows that DNA-PK loss does not confer a general defect on intracellular innate immune signalling, and the same is true in cultured Prkdc−/− MEFs which respond normally to RNA. It was noticed that the defect in the innate immune response to DNA was greater in cells lacking DNA-PKcs than in cells lacking Ku, even though Ku can still bind DNA in the absence of DNA-PKcs. This suggests that signalling can progress with DNA-PKcs interacting with DNA in the absence of Ku but that Ku enhances the signalling process by increasing the affinity of the protein complex for DNA. The phenotypic defect in DNA sensing was consistent in cells from four separate genotypes, ruling out the possibility that it was caused by a second site mutation. Consistent with this reintroduction of Ku80 into Xrcc5−/− cells restored DNA sensing (Figure 2J).

14The well-studied PrkdcSCID mutation kills the kinase activity of DNA-PKcs via the introduction of a premature stop codon that results in expression of a truncated protein. Although kinase activity is essential for DNA-PKcs to function in DNA repair, and therefore V(D)J recombination, this mutation did not affect the ability of DNA-PK to function as an innate immune DNA sensor. The functional significance of the interaction of DNA-PK with IRF-3 and its subsequent N-terminal phosphorylation reported by remains unknown. However, we show here that Ku interacts with STING in resting cells and that this interaction is abrogated upon DNA stimulation (Figure 5F). In the future, further work is necessary to understand how TBK-1 and STING contribute to the full activation of IRF-3 following binding of DNA to DNA-PK to allow IRF-3 translocation to occur.

15DNA can act as a powerful immunostimulatory agent in many contexts. DNA vaccination relies on DNA sensing to invoke a powerful innate immune response that, in turn, assists the adaptive response. Understanding how to optimise such vaccines, therefore, relies on understanding the mechanisms of detection of DNA by the immune system. Furthermore, one of the most common vaccine adjuvants, alum, acts by stimulating the release of DNA from neutrophils. Neutrophil extracellular traps (NETs) consist of webs of DNA with globular proteins, are released by a specific form of cell death and function as antimicrobial traps, thereby contributing to the innate immune response.

16Outside the context of infection, DNA can act as a damage-associated molecular pattern (DAMP), accelerating inflammatory responses following its release from dying or damaged cells directly contributing to the pathogenesis of various diseases such as atherosclerosis and deep vein thrombosis. The ability of DNA to act as a DAMP may also link nucleic acid sensing to several autoimmune disorders. In general, autoimmune conditions are characterised by immune responses against host molecules and tissues and are frequently associated with inflammation. Although the causative factors of many such conditions are incompletely understood, it is clear that deregulation of immune signalling may lead to autoinflammation and autoimmunity in some instances. Indeed, the accumulation of DNA and its subsequent detection by DNA sensing pathways can result in the initiation of autoimmune diseases. Mice which lack either of two enzymes responsible for degradation of DNA, 3′ repair exonuclease 1 (Trex1) and DNaseII, develop spontaneous autoimmune disorders associated with the initiation of IRF-3-dependent cytosolic DNA sensing. The absence of DNaseII results in chronic polyarthritis which is thought to be a result of the inflammation caused by a lack of clearance of DNA from macrophages. Trex1 deficiency or mutation, on the other hand, is associated with systemic lupus erythematosus (SLE), chilblain lupus and the human disease Aicardi-Goutières syndrome (AGS) and in this case inflammation is initiated from non-haematopoietic cells via a STING-dependent pathway. Interestingly, defects in the clearance of NETs have also been suggested to contribute to the initiation of SLE. Furthermore, the up-regulation of the inflammasome-activating DNA sensor absent in melanoma 2 (AIM2) in a mouse model of lupus and in patients with SLE-associated nephritis and the presence of anti-DNA-PK and anti-RNA-Pol III antibodies in patients with SLE and systemic sclerosis makes the link between DNA sensing and autoimmune disorders worthy of further investigation.

17The identification of several candidate DNA sensors in multiple cells types in recent years suggests the evolution of redundancy in this system. This redundancy is not surprising given the tendency of pathogens to evolve escape-mechanisms for evading host immune mediators in turn inducing the host to evolve further pathogen recognition mechanisms. In the context of DNA sensing this is exemplified by the relatively recent evolution of the PYHIN domain proteins, such AIM2 and IFI16, which are a mammalian addition to the ancient innate immune system. Furthermore, a function of DAI was recently uncovered by identifying murine cytomegalovirus protein, vIRA, which interacts with DAI and inhibits its ability to initiate DNA-induced necroptosis. The biological function for DAI may therefore be in the initiation of a cell death pathway, rather than an IRF-3 dependent inflammatory response. This indicates there are at least three outcomes to cytosolic DNA sensing, the induction of cytokine expression via IRF-3 and NF-kB activation, the secretion of IL-1β via the AIM 2 inflammasome and the induction of necroptosis by DAI. What is not clear though, is how these different responses contribute to the overall immune response to infection by DNA pathogens and to what extent they are cell and tissue-type dependent. Further work is necessary to uncover the relative contributions of these different DNA sensing mechanisms in specific cell types and to different DNA structures as well as to understand how these sensors co-ordinate with STING and other adaptor proteins to activate TBK1 and IRF-3.

18Overall these findings provide a novel function for DNA-PK in the innate immune response, beyond its roles in DNA repair and V(D)J recombination, and increase our understanding of the innate immune response to cytoplasmic DNA.

19Prkdc+/− mice on a 129 background were a kind gift from Dr Fred Alt and PrkdcSCID and Balb/c mice were from Harlan laboratories. Animals were maintained as required under UK Home Office regulations. Groups of 5 age and sex matched mice were injected intradermally with 1 µg DNA or poly(I:C) (Invivogen, San Diego, CA) pre-incubated with 2 μl Lipofectamine2000 in Optimem (Life Technologies, Grand Island, NY) or injected intradermally with 106 plaque forming units (pfu) of MVA, HSV-1 or influenza virus A/PR/8/34 in PBS. Primary mouse embryonic fibroblasts (MEFs) were isolated from E13.5 embryos derived from time-mated pregnant mice using standard protocols.

20HEK293T and HEK293 Trex cells (Life Technologies, Grand Island, NY) were maintained in DMEM containing 10% FBS with the addition of blasticidin (5 μg/ml) and zeocin (100 μg/ml) for the selection and maintenance of the inducible STING-expressing cell line (STING-293Trex). MEFs and murine skin fibroblasts (MSFs) from various genetic backgrounds were maintained in DMEM containing 15% FBS, 100 U/ml penicillin and 100 μg/ml streptomycin. Primary Prkdc+/+ and Prkdc−/− MEFs were prepared in house on multiple occasions or supplied as a kind gift by Dr Brian Hemmings and were used only at passage 1. Transformed Prkdc−/− MEFs (used solely for experiments leading to the data presented in Figure 2D) were a kind gift from Professor Penelope Jeggo. HeLa cells were maintained in RPM.I containing 10% FBS and 2 mM L-glutamine. Transfections were carried out with Fugene6 (Roche, Penzburg, Germany).

21Double stranded oligonucleotide DNA, ISD (sense sequence, TACAGATCTACTAGTGATCTATGACTGATCTGTACATGATCTACA) was phosphorylated at the 5′ end by incubation with T4 polynucleotide kinase (New England Biolabs, Ipswich, MA) for 30 min at 37°C and then ligated with T4 DNA ligase (Promega, Madison, WI) for 16 hr at 15°C. Concatenation was confirmed by agarose gel electrophoresis. This DNA was used at 10 μg/ml for transfection unless stated otherwise.

223′ biotinylated oligonucleotides were purchased from IDT DNA Technologies. Other nucleic acids were biotinylated using the Photoprobe biotinylation kit (Vector Labs, Burlingame, CA) following the manufacturer's instructions.

23Concatenated oligonucleotide DNA (sequence as above) that was biotinylated at the 3′ end was transfected into cells using PEI (Sigma-Aldrich, St Louis, MI). After 30 min, cells were lysed in buffer containing 10 mM Tris–Cl, pH 8, 0.1% NP40, 10 mM MgCl2 and the cytoplasmic fraction was isolated by centrifugation at 1500g for 3 min. Streptavidin agarose (Thermo Scientific, Rockford, IL), 30 μl, was incubated with the lysate for 1 hr at 4°C and then washed three times in PBS. Purified proteins were analysed by SDS-PAGE and immunoblotting or stained by coomassie-blue and identified by liquid chromatography and tandem mass spectrometry (LC-MS/MS) at the Centre for Systems Biology at Imperial College London.

24VACV strain MVA was purified from cytoplasmic extracts of infected BHK-21 cells by sedimentation through a cushion of 36% (wt/vol) sucrose and was titrated by plaque assay on chicken embryo fibroblasts. NDV and influenza virus strain A/PR/8/34 were kind gifts from Prof Wendy Barclay. HSV-1 strain S17, a gift from Dr Colin Crump, was grown in Vero cells and purified on ficoll gradients. These viruses were used for infections for the indicated times and at indicated doses.

25For immunoblotting, cell lysates were separated by polyacrylamide gel electrophoresis and transferred onto Immobilon P membranes (GE Healthcare, Little Chalfont, UK). The membranes were blocked in 5% non-fat milk in TBS containing 0.1% Tween 20 for 1 hr at room temperature. Membranes were probed with antibodies against Ku70 (Abcam, Cambridge, UK), Ku80 (Santa Cruz Biotech, Santa Cruz, CA), DNA-PKcs (Millipore, Billerica, MA), tubulin (Millipore, Billerica, MA), histone H3 (Millipore, Billerica, MA), hIRF-3 (Santa Cruz Biotech, Santa Cruz, CA), p-IRF-3 (serine 396, Abcam, Cambridge, UK), HMGB1 (Abcam, Cambridge, UK), FLAG (Sigma-Aldrich, St Louis, MI) or VACV strain Western Reserve and bound immunoglobulin was detected with horse-radish peroxidase-linked secondary antibodies (Agilent, Santa Clara, CA). Ku80, IRF-3 (Life Technologies, Grand Island, NY) or control antibodies were used for immunoprecipitation from HeLa cell lysates expressing IRF-3. FLAG-agarose matrix (Sigma-Aldrich, St Louis, MI) was used for immunoprecipitation of STING from STING-293Trex cells. For immunofluorescence, cells were seeded onto 15 mm glass coverslips, infected with MVA at 5 pfu per cell for 6 hr or transfected and fixed with 4% paraformaldehyde. Cells were permeabilised with PBS containing 0.2% Triton-X100 and blocked with 5% non-fat milk in PBS containing 0.1% Tween 20 for 1 hr at 20°C. Incubation with primary antibodies against Ku70 (Abcam, Cambridge, UK), DNA-PKcs (Millipore, Billerica, MA), mIRF-3 (Life Technologies, Grand Island, NY) hIRF-3, p65 (both Santa Cruz Biotech, Santa Cruz, CA) or TBK1 (Cell Signalling, Danvers, MA) diluted in PBS with 1% milk, for 1 hr at 20°C was followed by detection with alexa-fluor-conjugated secondary antibodies (Life Technologies, Grand Island, NY). Cells were counterstained with DAPI and mounted with Mowiol. Images were obtained with a Zeiss Pascal 510 confocal microscope and processed with Zeiss LSM software (Zeiss, Oberkochen, Germany). For quantification of translocation or IRF-3 or NF-κB into the nucleus 50 cells were counted in random fields of view, in biological triplicates, for each condition and scored for the presence of nuclear staining.

26Levels of Cxcl10 and Ifnβ in cell supernatants were measured using ELISA kits (R&D systems, Minneapolis, MN or PBL, Piscataway, NJ, respectively) according to the manufacturer's instructions.

27Total cellular RNA was extracted using an RNeasy kit (Qiagen, Hilden, Germany). cDNA synthesis was carried out with Superscript III Reverse Transcriptase (Life Technologies, Grand Island, NY) using 500 ng of template RNA. qPCR was performed on a 7900HT series thermocycler (Life Technologies, Grand Island, NY) with Fast SYBR Green Master Mix (Life Technologies, Grand Island, NY). HPRT was used as the reference gene in all assays. Data were analysed with RQ manager 1.2 software (Life Technologies, Grand Island, NY) and presented as a fold increase relative to time zero. Primers for qPCR were as follows:Cxcl10 For 5′ ACTGCATCCATATCGATGAC 3′,Cxcl10 Rev 5′ TTCATCGTGGCAATGATCTC 3′,Ifnβ For 5′ CATCAACTATAAGCAGCTCCA 3′,Ifnβ Rev 5′ TTCAAGTGGAGAGCAGTTGAG 3′Ccl5 For 5′ ACGTCAAGGAGTATTTCTACAC 3′,Ccl5 Rev 5′ GATGTATTCTTGAACCCACT 3′,Il-6 For 5′ GTAGCTATGGTACTCCAGAAGAC 3′,Il-6 Rev 5′ GTAGCTATGGTACTCCAGAAGAC 3′,Cxcl2 For 5′ GAGCTTGAGTGTGACGCCCCC 3′,Cxcl2 Rev 5′ GTTAGCCTTGCCTTTGTTCAG 3′,Ccl3 For 5′ ACTGCCTGCTGCTTCTCCTA 3′,Ccl3 Rev 5′ TTGGAGTCAGCGCAGATCTG 3′,Ccl4 For 5′ GCCCTCTCTCTCCTCTTGCT 3′,Ccl4 Rev 5′ CTGGTCTCATAGTAATCCATC 3′,Ccl2 For 5′ CTTCTGGGCCTGCTGTTCA 3′,Ccl2 Rev 5′ CCAGCCTACTCATTGGGATCA3′,Ifnγ For 5′ TCAAGTGGCATAGATGTGGAAGAA3′Ifnγ Rev 5′ TGGCTCTGCAGGATTTTCATG 3′,Il-4 For 5′ CATGCACGGAGATGGATG 3′,Il-4 Rev 5′ ACCTTGGAAGCCCTACAGAC 3′,Il-10 For 5′ TCCTTAATGCAGGACTTTAAGGGTTACTTG 3′,Il-10 Rev 5′ GACACCTTGGTCTTGGAGCTTATTAAAATC 3′,HPRT For 5′ GTTGGATACAGGCCAGACTTTGTTG 3′,HPRT Rev 5′ GATTCAACTTGCGCTCATCTTAGGC 3′,Nfkbia For 5′ CTGCAGGCCACCAACTACAA3′,Nfkbia Rev 5′ CAGCACCCAAAGTCACCAAGT 3′Isg54 For 5′ ATGAAGACGGTGCTGAATACTAGTGA 3′Isg54 Rev 5′ TGGTGAGGGCTTTCTTTTTCC 3′

28Cxcl10 For 5′ ACTGCATCCATATCGATGAC 3′,

29Cxcl10 Rev 5′ TTCATCGTGGCAATGATCTC 3′,

30Ifnβ For 5′ CATCAACTATAAGCAGCTCCA 3′,

31Ifnβ Rev 5′ TTCAAGTGGAGAGCAGTTGAG 3′

32Ccl5 For 5′ ACGTCAAGGAGTATTTCTACAC 3′,

33Ccl5 Rev 5′ GATGTATTCTTGAACCCACT 3′,

34Il-6 For 5′ GTAGCTATGGTACTCCAGAAGAC 3′,

35Il-6 Rev 5′ GTAGCTATGGTACTCCAGAAGAC 3′,

36Cxcl2 For 5′ GAGCTTGAGTGTGACGCCCCC 3′,

37Cxcl2 Rev 5′ GTTAGCCTTGCCTTTGTTCAG 3′,

38Ccl3 For 5′ ACTGCCTGCTGCTTCTCCTA 3′,

39Ccl3 Rev 5′ TTGGAGTCAGCGCAGATCTG 3′,

40Ccl4 For 5′ GCCCTCTCTCTCCTCTTGCT 3′,

41Ccl4 Rev 5′ CTGGTCTCATAGTAATCCATC 3′,

42Ccl2 For 5′ CTTCTGGGCCTGCTGTTCA 3′,

43Ccl2 Rev 5′ CCAGCCTACTCATTGGGATCA3′,

44Ifnγ For 5′ TCAAGTGGCATAGATGTGGAAGAA3′

45Ifnγ Rev 5′ TGGCTCTGCAGGATTTTCATG 3′,

46Il-4 For 5′ CATGCACGGAGATGGATG 3′,

47Il-4 Rev 5′ ACCTTGGAAGCCCTACAGAC 3′,

48Il-10 For 5′ TCCTTAATGCAGGACTTTAAGGGTTACTTG 3′,

49Il-10 Rev 5′ GACACCTTGGTCTTGGAGCTTATTAAAATC 3′,

50HPRT For 5′ GTTGGATACAGGCCAGACTTTGTTG 3′,

51HPRT Rev 5′ GATTCAACTTGCGCTCATCTTAGGC 3′,

52Nfkbia For 5′ CTGCAGGCCACCAACTACAA3′,

53Nfkbia Rev 5′ CAGCACCCAAAGTCACCAAGT 3′

54Isg54 For 5′ ATGAAGACGGTGCTGAATACTAGTGA 3′

55Isg54 Rev 5′ TGGTGAGGGCTTTCTTTTTCC 3′

56Statistical analysis was carried out using student's t-test with Welch's correction where necessary.