Micropropagation of selected Oenothera species and preliminary studies on their secondary metabolites

A method was devised for the micropropagation of eight species of the genus Oenothera L. from shoot tips and shoot segments with nodes. Microshoot cultures were obtained from explants on Murashige and Skoog (MS) medium enriched with IAA and BA. Numerous shoots developed properly after they had been transferred onto MS medium without BA. When they had rooted under the influence of auxins (IAA or IBA), shoots were transferred to pots and then to the soil, where they matured. In the seeds produced by these plants, the content of fatty acids was determined using the GC method. A preliminary analysis of flavonoid compounds and phenolic acids was made using the 2D TLC method (fingerprinting) in microshoots and leaves of soil plants regenerated in vitro.

Abstract licence: CC BY

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