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Immunological comparison of the NADH:nitrate reductase from different cucumber tissues

Jolanta Marciniak, Grażyna Kłobus, Józef Buczek and 2 more

Acta Societatis Botanicorum Poloniae · 2014

doi:10.5586/asbp.1995.032 (opens in a new tab)

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Abstract

Soluble nitrate reductase from cucumber roots ( Cucumis sativus L.) was isolated and purified with blue-Sepharose 4B. Specific antibodies against the NR protein were raised by immunization of a goat. Using polyclonal antibodies anti-NR properties of the nitrate reductase from various cucumber tissues were examined. Experiments showed difference in immuno-logical properties of nitrate reductase (NR) from cotyledon roots and leaves.

Abstract licence: CC BY

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