Aluminium effects on pyridine nucleotide redox state in roots of Scots pine

After prolonged (3-9 weeks) hydroponic treatment of Scots pine seedlings with different concentrations (0.5-4.0 mM) of Al (AI(N0 3 ) 3 ), the levels of pyridine nucleotides were determined in root homogenates. After 3 weeks of Al stress, a significant decrease of the anabolic reduction charge (ARC: NADPH/(NADP + + NADPH)) and an increase of the redox status (NAD(P)H/NAD(P) + ), catabolic reduction charge (CRC: NADH/(NAD + + NADH)) and phosphorylation capacity expressed as NADP + /NAD + ratio was found in the 4.0 mM Al treatment. After 6 weeks, Al at concentrations of 0.5 and 1.0 mM induced an enhancement of the NADH level and a reduction of NADPH level, but the redox ratios were not changed significantly. After 9 weeks treatment with Al concentrations ranging from 0.5 to 4.0 mM, decreases of the relative level of NADP + , NADPH and NADH and increases of NAD + were found. Consequently, the CRC, NAD(P)H/NAD(P) + and NADP + /NAD + ratios reached a minimum and ARC a maximum as compared to previous measurements.

Abstract licence: CC BY

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