Detection of potato virus X in potato leaves by means of polyacrylamide gel electrophoresis
Marta Czupryn, Maria Błaszczyk
Acta Societatis Botanicorum Poloniae · 2015
doi:10.5586/asbp.1974.049 (opens in a new tab)
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Abstract
A rapid method of detection and evaluation of potato virus X (PVX) infection in potato leaves is described. The method is based on: 1) discarding of major part of cytoplasmic proteins from leaf homogenate by precipitation with polyethylene glycol in final concentration of 2%, 2) precipitation of virus containing fraction by increasing the concentration of polyethylene glycol to 3.5% and electrophoretic identification of virus coat protein extracted from this fraction. The procedure allows early detection of PVX in plants inoculated with virus as well as grown from infected tubers.
Abstract licence: CC BY
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