Effect of juglone on C-32 and COLO 829 melanoma cells in in vitro cultures

Juglone is an allelopathin secreted by black walnut tree of the Juglandaceae family and is used as an active in�gre�dient in many herbal preparations and as a commercial dye. It is considered as an important phytochemical with wide therapeutic potential. Black walnut extract has long been used in folk medicine to treat various types of can�cers. It demonstrates antiviral, antibacterial, antifungal, and antitumor activities. The present study aimed to ana�lyze the effect of juglone on the viability and proliferation of melanoma cells of C-32 (amelanotic melanoma) and COLO 829 (melanotic melanoma) cell lines in vitro and on the mRNA expression of genes encoding the pro�apo�ptotic BAX protein and caspase 3 and the gene encoding antiapoptotic BCL2 protein. The results showed a dose-dependent effect of juglone on the viability, proliferation, and death induction in C-32 and COLO 829 mela�noma cells and in HFF-1 normal dermal fibroblasts in in vitro cultures, but melanoma cells were more sensi�tive to juglone. Our findings revealed different mRNA expression patterns for all the studied genes in melanoma and normal cells treated with juglone in in vitro cultures.

Abstract licence: CC BY

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