Investigation of gene expression in primary embryonic cell line (FGBC8) from olive flounder Paralichthys olivaceus
Ju-Won Kim, Ja Young Cho, Won-Kyong Chun and 5 more
Fisheries and Aquatic Sciences · 2021
doi:10.47853/fas.2021.e36 (opens in a new tab)
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Abstract
In this paper, we have reported the expression of immune-related gene in a new embryonic cell line (FGBC8) which was established from olive flounder (Paralichthys olivaceus) embryos. To explore the cell biotechnological applicability, the FGBC8 cells were incubated with the several mitogens such as lipopolysaccharide (LPS), polyinosinic-polycytidylic acid (poly I:C), flagellin,and interferon (IFN)-γ. After incubation, the expression of immune-related gene was observed in FGBC8 cells through the quantitative real-time PCR. Our results indicate that FGBC8 cells will serve as a valuable research tool for investigating host-pathogen interactions as well as cell biotechnological applications.
Abstract licence: CC BY-NC
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