Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
Tshiphiri Senamela, Marleen Kock, Piet Becker and 1 more
African Journal of Laboratory Medicine · 2018
doi:10.4102/ajlm.v7i1.658 (opens in a new tab)
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Abstract
The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation.
Abstract licence: CC BY
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