In vitro activity of Pithecellobium dulce and Lysiloma acapulcensis on exogenous development stages of sheep gastrointestinal strongyles

An experiment was conducted to evaluate the effects of two lyophilised aqueous extracts of <em>Lysiloma acapulcensis</em> (LAE) and <em>Phitecellobium dulce</em> (PDE) tree leaves on <em>in vitro</em> assessment of hatching of eggs, larval development and migration of gastrointestinal nematodes of sheep using a general linear model. Treatments contained extracts from both species at concentrations of 0, 125, 250 and 500 μg/mL. Both albendazole and levamisole were used at a level of 1% as positive control. The extract of LAE, compared to PDE, showed better inhibition (P&lt;0.05) of egg hatching. Different doses of both the LAE and PDE extracts showed a larvicidal effect (P&lt;0.05) on all larvae exposed to different doses of the extracts. In the larval migration assay, a similar effect with levamisole at doses of 250 and 500 μg/mL occurred with the LAE extract. The extract of <em>P. dulce</em> had a lower larvicidal effect (P&lt;0.05) than levamisole and <em>L. acapulcensis</em> extracts. Using aqueous extracts of both species of <em>L. acapulcensis</em> and <em>P. dulce</em> could be a promising alternative to synthetic anthelmintics as treatments of gastrointestinal nematodes of sheep in organic and conventional production systems under subtropical conditions.

Abstract licence: CC BY-NC

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