Development and Characterization of 10 Microsatellite Markers in Sagina nodosa (Caryophyllaceae)
Tarjinder Singh, Joan Edwards, Luana S. Maroja
Applications in Plant Sciences · 2014
doi:10.3732/apps.1300064 (opens in a new tab)
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Abstract
Premise of the study: We developed 10 novel microsatellite loci for <i>Sagina nodosa</i>, a diploid perennial arctic-alpine herb. To our knowledge, these are the first microsatellite loci for a <i>Sagina</i> species. Methods and Results: We performed a low-coverage 454 next-generation sequencing of enriched genomic fragments derived from one individual to generate a massive library of contigs containing potential polymorphic microsatellites. We present data for 10 novel polymorphic microsatellite loci containing di-, tri-, tetra-, and hexanucleotide repeats with two to nine alleles per locus assessed in 29 individuals. Conclusions: These polymorphic microsatellite loci in <i>S. nodosa</i> will provide insights on the population structure and life history of <i>S. nodosa</i> in Isle Royale and other North American populations.
Abstract licence: CC BY-NC
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