Standardization of sample collection, isolation and analysis methods in extracellular vesicle research

Abstract

The emergence of publications on extracellular RNA (exRNA) and extracellular vesicles (EV) has highlighted the potential of these molecules and vehicles as biomarkers of disease and therapeutic targets. These findings have created a paradigm shift, most prominently in the field of oncology, prompting expanded interest in the field and dedication of funds for EV research. At the same time, understanding of EV subtypes, biogenesis, cargo and mechanisms of shuttling remains incomplete. The techniques that can be harnessed to address the many gaps in our current knowledge were the subject of a special workshop of the International Society for Extracellular Vesicles (ISEV) in New York City in October 2012. As part of the “ISEV Research Seminar: Analysis and Function of RNA in Extracellular Vesicles (evRNA)”, 6 round‐table discussions were held to provide an evidence‐based framework for isolation and analysis of EV, purification and analysis of associated RNA molecules, and molecular engineering of EV for therapeutic intervention. This article arises from the discussion of EV isolation and analysis at that meeting. The conclusions of the round table are supplemented with a review of published materials and our experience. Controversies and outstanding questions are identified that may inform future research and funding priorities. While we emphasize the need for standardization of specimen handling, appropriate normative controls, and isolation and analysis techniques to facilitate comparison of results, we also recognize that continual development and evaluation of techniques will be necessary as new knowledge is amassed. On many points, consensus has not yet been achieved and must be built through the reporting of well‐controlled experiments.

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     Not in our library

237. 237.
     The Majority of MicroRNAs Detectable in Serum and Saliva Is Concentrated in Exosomes · 2012

     No notice
238. 238.

     This reference’s wording can’t be shown under the paper’s licence.

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239. 239.

     This reference’s wording can’t be shown under the paper’s licence.

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240. 240.

     This reference’s wording can’t be shown under the paper’s licence.

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241. 241.

     This reference’s wording can’t be shown under the paper’s licence.

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242. 242.

     This reference’s wording can’t be shown under the paper’s licence.

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243. 243.

     This reference’s wording can’t be shown under the paper’s licence.

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244. 244.

     This reference’s wording can’t be shown under the paper’s licence.

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245. 245.

     This reference’s wording can’t be shown under the paper’s licence.

     Not in our library

246. 246.

     This reference’s wording can’t be shown under the paper’s licence.

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247. 247.

     This reference’s wording can’t be shown under the paper’s licence.

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248. 248.

     This reference’s wording can’t be shown under the paper’s licence.

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249. 249.

     This reference’s wording can’t be shown under the paper’s licence.

     Not in our library

250. 250.

     This reference’s wording can’t be shown under the paper’s licence.

     Not in our library

251. 251.

     This reference’s wording can’t be shown under the paper’s licence.

     Not in our library

252. 252.
     EVpedia: an integrated database of high‐throughput data for systemic analyses of extracellular vesicles · 2013

     No notice
253. 253.

     This reference’s wording can’t be shown under the paper’s licence.

     Not in our library

254. 254.
     Body fluid derived exosomes as a novel template for clinical diagnostics · 2011

     No notice
255. 255.

     This reference’s wording can’t be shown under the paper’s licence.

     Not in our library

256. 256.

     This reference’s wording can’t be shown under the paper’s licence.

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257. 257.

     This reference’s wording can’t be shown under the paper’s licence.

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258. 258.

     This reference’s wording can’t be shown under the paper’s licence.

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259. 259.

     This reference’s wording can’t be shown under the paper’s licence.

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260. 260.

     This reference’s wording can’t be shown under the paper’s licence.

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261. 261.

     This reference’s wording can’t be shown under the paper’s licence.

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262. 262.

     This reference’s wording can’t be shown under the paper’s licence.

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263. 263.

     This reference’s wording can’t be shown under the paper’s licence.

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264. 264.

     This reference’s wording can’t be shown under the paper’s licence.

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265. 265.

     This reference’s wording can’t be shown under the paper’s licence.

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266. 266.

     This reference’s wording can’t be shown under the paper’s licence.

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267. 267.

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268. 268.

     This reference’s wording can’t be shown under the paper’s licence.

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269. 269.

     This reference’s wording can’t be shown under the paper’s licence.

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270. 270.

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271. 271.

     This reference’s wording can’t be shown under the paper’s licence.

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272. 272.

     This reference’s wording can’t be shown under the paper’s licence.

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273. 273.

     This reference’s wording can’t be shown under the paper’s licence.

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274. 274.

     This reference’s wording can’t be shown under the paper’s licence.

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275. 275.

     This reference’s wording can’t be shown under the paper’s licence.

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276. 276.

     This reference’s wording can’t be shown under the paper’s licence.

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277. 277.

     This reference’s wording can’t be shown under the paper’s licence.

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278. 278.

     This reference’s wording can’t be shown under the paper’s licence.

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279. 279.

     This reference’s wording can’t be shown under the paper’s licence.

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280. 280.

     This reference’s wording can’t be shown under the paper’s licence.

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281. 281.

     This reference’s wording can’t be shown under the paper’s licence.

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282. 282.

     This reference’s wording can’t be shown under the paper’s licence.

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283. 283.

     This reference’s wording can’t be shown under the paper’s licence.

     Not in our library

Cited by papers we hold 116

• Emerging biomarkers for early cancer detection and diagnosis: challenges, innovations, and clinical perspectives · 2025
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• Extracellular vesicle-based drug overview: research landscape, quality control and nonclinical evaluation strategies · 2025
  Corrected
• Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches · 2024
  No notice
• Extracellular vesicles, RNA sequencing, and bioinformatic analyses: Challenges, solutions, and recommendations · 2024
  No notice
• Extracellular Vesicles in Liquid Biopsies as Biomarkers for Solid Tumors · 2023
  No notice
• Mesenchymal stem cell-derived extracellular vesicles/exosome: A promising therapeutic strategy for intracerebral hemorrhage · 2023
  Not checked
• Recent advances of small extracellular vesicle biomarkers in breast cancer diagnosis and prognosis · 2023
  No notice
• Tackling the effects of extracellular vesicles in fibrosis · 2022
  Not checked
• Oral administration of turmeric-derived exosome-like nanovesicles with anti-inflammatory and pro-resolving bioactions for murine colitis therapy · 2022
  No notice
• Exosomes and cancer - Diagnostic and prognostic biomarkers and therapeutic vehicle · 2022
  No notice
• Knowledge Mapping of Exosomes in Autoimmune Diseases: A Bibliometric Analysis (2002–2021) · 2022
  No notice
• Extracellular vesicles in ovarian cancer chemoresistance, metastasis, and immune evasion · 2022
  No notice
• Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma – Contributions of platelet extracellular vesicles in plasma samples · 2022
  No notice
• Bovine Milk-Derived Exosomes as a Drug Delivery Vehicle for miRNA-Based Therapy · 2021
  No notice
• Extracellular vesicle concentrations of glial fibrillary acidic protein and neurofilament light measured 1 year after traumatic brain injury · 2021
  Not checked
• Critical Review of the Evolution of Extracellular Vesicles’ Knowledge: From 1946 to Today · 2021
  No notice
• Isolation and analysis methods of extracellular vesicles (EVs) · 2021
  No notice
• Coupling size exclusion chromatography to ultracentrifugation improves detection of exosomal proteins from human plasma by LC-MS · 2021
  Not checked
• Extracellular Vesicles and Exosomes: Insights From Exercise Science · 2021
  No notice
• Engineered exosomes: desirable target-tracking characteristics for cerebrovascular and neurodegenerative disease therapies · 2021
  No notice

and 96 more

Reads

A person counts once a day per paper. We keep no cookie and no address: a reader is a hash that changes every day. Any month under five reads shows no number.