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Chikungunya Virus–Vector Interactions

Lark Coffey, Anna-Bella Failloux, Scott Weaver

Viruses · 2014 · Review · CC BY

Abstract

Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes chikungunya fever, a severe, debilitating disease that often produces chronic arthralgia. Since 2004, CHIKV has emerged in Africa, Indian Ocean islands, Asia, Europe, and the Americas, causing millions of human infections. Central to understanding CHIKV emergence is knowledge of the natural ecology of transmission and vector infection dynamics. This review presents current understanding of CHIKV infection dynamics in mosquito vectors and its relationship to human disease emergence. The following topics are reviewed: CHIKV infection and vector life history traits including transmission cycles, genetic origins, distribution, emergence and spread, dispersal, vector competence, vector immunity and microbial interactions, and co-infection by CHIKV and other arboviruses. The genetics of vector susceptibility and host range changes, population heterogeneity and selection for the fittest viral genomes, dual host cycling and its impact on CHIKV adaptation, viral bottlenecks and intrahost diversity, and adaptive constraints on CHIKV evolution are also discussed. The potential for CHIKV re-emergence and expansion into new areas and prospects for prevention via vector control are also briefly reviewed.

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From PubMed Central, U.S. National Library of Medicine, as current on Oct 26, 2026. Paragraph numbers are ours, so an answer can point at one.

1. Introduction

1Emerging and re-emerging arthropod-borne viruses (arboviruses) represent a significant threat to human and veterinary health worldwide. Chikungunya virus (CHIKV), a mosquito-borne alphavirus that causes chikungunya fever (CHIK), a severe, debilitating and often chronic arthralgia, is a prime example; since it was first isolated in 1952, the virus has been detected as the etiologic agent of sporadic epidemics in Africa and Asia and, since 2004, has expanded its geographic range to circulate on Indian Ocean islands, and in Italy, France, and the Americas. CHIKV has also re-emerged in Southeast Asia since 2006, causing an estimated 1.3 million human cases. CHIKV cycles in urban settings between humans and two mosquito species found in the U.S., suggesting a potential for endemic establishment there. As evidence of this, 11 autochthonous cases of CHIK were detected in south Florida as of November 2014. Emergence of arboviruses like CHIKV underscores the interconnectedness of humans with their environments, and highlights our vulnerabilities to new disease threats posed by spreading viruses. Understanding how arboviruses like CHIKV emerge is critical to predict and prevent or mitigate human disease. Central to understanding emergence is knowledge of the natural ecology of CHIKV transmission and the dynamics of vector infections. Here we discuss current knowledge of CHIKV infection dynamics in mosquito vectors and its relationship to human disease emergence.

2.1. Chikungunya Virus Transmission Cycles and Genetic Origins

2Chikungunya virus is endemic in countries of Sub-Saharan Africa, India and Southeast Asia. The virus circulates in an enzootic cycle in Africa between forest-dwelling mosquitoes and non-human primates. Phylogenetic studies reveal the existence of two major enzootic CHIKV lineages in Africa: Western, and East/Central/South African (ECSA). In Asia, where the first outbreak was reported in 1958 in Thailand, CHIKV has historically been maintained in an urban cycle transmitted to humans by the mosquito Aedes (Stegomyia) aegypti and, to a lesser extent, Aedes (Stegomyia) albopictus.

2.2. Chikungunya Distribution, Emergence and Spread

3In 2004, CHIKV belonging to the ECSA lineage emerged from Lamu and Mombasa in coastal Kenya and spread to Comoros and later, to other islands of the Indian Ocean including La Réunion (Figure 1). There, the predominant vector A. albopictus transmitted preferentially a CHIKV variant with a single amino acid change from an alanine (A) to valine (V) at E1 envelope glycoprotein amino acid 226 of the ECSA Indian Ocean lineage (IOL) genotype. The E1-226V variant was more efficiently transmitted by A. albopictus, with roughly 40-fold more efficient initial infection of midgut epithelial cells. The selection of the E1-226V variant occurs at the initial infection of the midgut of A. albopictus, leading to a higher viral dissemination and transmission of the IOL genotype by this mosquito. A series of four additional adaptive mutations in the E2 gene have also been incriminated more recently in enhancing transmission by A. albopictus. During 2005–2006, the virus spread to neighboring Indian Ocean islands including Mayotte, Mauritius and Madagascar, where CHIKV E1-A226V was mainly transmitted by A. albopictus. Subsequently, the CHIKV IOL was introduced to India and the surrounding islands, Sri Lanka and the Maldives. In Africa, the CHIKV E1-226V variant was also implicated outbreaks in Cameroon, Gabon and Congo. This variant also caused the first European CHIKV outbreak in Italy in 2007. Since 2008, IOL CHIKV strains were also imported into Southeast Asia: Malaysia, Singapore, Thailand, China, Cambodia and Bhutan. Remarkably, the E1-226V variant was found preferentially in rural areas where A. albopictus was more abundant than A. aegypti, and presumably was the primary vector. In September 2010, autochthonous cases of CHIKV were reported in southeast of France, again with A. albopictus as the vector. In Southeast France, this species appears to behave differently compared to its tropical counterpart, as it efficiently transmits the E1-226A IOL variant detected in local circulation. Aedes albopictus has been found in in 18 French departments as well as 19 other countries in Europe.

4Prior to December 2013, CHIKV transmission was not documented in the Americas, despite numerous introductions and the presence of conditions that are apparently suitable for its establishment. In October 2013, two laboratory-confirmed, autochthonous CHIKV cases were detected in the French territory of Saint Martin Island, in the Caribbean Sea. Surprisingly, the CHIKV strain isolated belonged to the Asian genotype rather than the IOL that emerged in 2004 in the Indian Ocean Basin and Asia. This St. Martin strain was phylogenetically close to CHIKV identified in Indonesia in 2007, China in 2012 and Philippines in 2013, but more distant from the Asian genotype that circulated in New Caledonia. The only vector implicated in St. Martin, where A. albopictus has not been established, was A. aegypti. Very rapidly, an epidemic was established in the island and subsequently, CHIKV progressively spread throughout most of the Caribbean, and into Central and South America where human populations are mostly naïve to CHIKV.

5American populations of A. aegypti and A. albopictus are susceptible to both ECSA/IOL and Asian genotypes of CHIKV with higher susceptibility observed for A. aegypti and the Asian CHIKV genotype, or A. albopictus and the ECSA/IOL genotype. As of November 2014, 11 locally acquired cases of CHIKV have been documented in Florida, USA. These recent findings and the history of CHIKV movement suggest that it will continue to spread throughout the Americas and other tropical and subtropical regions of the world wherever mosquito vectors occur.

3.1. Chikungunya Vector Distribution

6CHIKV is an African virus that circulates enzootically in sylvatic cycle between arboreal, canopy-dwelling mosquitoes and non-human primates. Enzootic strains from the West African and ECSA lineages have been isolated from diverse mosquito species in Senegal, Ivory Coast, Central African Republic, Uganda and South Africa. In Senegal, CHIKV has been detected in vectors of yellow fever virus: Aedes (Diceromyia) furcifer, A. (Diceromyia) taylori, A. (Stegomyia) luteocephalus, A. (Stegomyia) africanus and A. (Stegomyia) neoafricanus. In South Africa, A. (Diceromyia) cordellieri, was also involved in CHIKV transmission. The urbanization of CHIKV, with epidemics occurring in peridomestic settings following the emergence of enzootic strains, coincides with the involvement of anthropophilic mosquitoes: A. (Stegomyia) aegypti (L.) and A. (Stegomyia) albopictus (Skuse), favoring inter-human transmission. Aedes aegypti, first described by Linné in 1862, originated in African forests and is today present in most tropical countries. In Africa, A. aegypti is present in two genetic forms: (i) the dark and sylvatic A. aegypti formosus, found in forested habitats and using treeholes as larval development sites; and (ii) the pale and domestic A. aegypti aegypti, which is widespread in the tropics and subtropics and uses artificial larval habitats mainly in urban environments. Aedes aegypti aegypti originated from the forest-dwelling formosus form, which may have spread from tropical African forests to North Africa where it probably became domesticated under pressure to use artificial water storage containers as larval habitats. These mosquitoes progressively differentiated into domesticated populations known as A. aegypti aegypti. Human trading activities later introduced this subspecies globally throughout the most of the tropics and subtropics: the New World via the African slave trade from the 15th to 19th centuries, Asia in the 18th and 19th centuries, and the Pacific islands with troop movements during World War II.

7Aedes aegypti can exist sympatrically with A. albopictus and also often shares larval habitats. Aedes albopictus, originally described by Skuse in Calcutta, India in 1894 originated in forests of Southeast Asia, but is now is commonly found in peri-urban, rural and forested areas on five continents. Aedes albopictus has no particular ecological specialization, colonizing both temperate and tropical regions. Two types of populations are described: (1) temperate populations imported to the U.S. from Japan and then from the U.S. to Europe, where they are now established in 20 European countries; these temperate populations are characterized by diapausing, cold-resistant eggs; and (2) tropical populations. Aedes albopictus is a competent vector for at least 26 arboviruses and is implicated occasionally in DENV (e.g., on the Seychelles islands; and more frequently in CHIKV transmission (e.g., on La Réunion Island; see Table 1 of this review as well). Since the 2007 outbreak of CHIKV in Italy, Europe is considered vulnerable for transmission of several “tropical” arboviruses, particularly in regions where A. albopictus is present.

3.2. Vector Dispersal and Genetics

8Aedes aegypti and A. albopictus have both spread beyond their native ranges via commercial trade and dessication-resistant eggs. Aedes aegypti largely replaced A. albopictus in Southeast Asian cities in the first half of the 20th century while the introduction of A. albopictus into the Americas during the 1980s was associated with a decline in the abundance of A. aegypti in some regions such as occurred in the Americas in the 1980s and is now ongoing in Central Africa and on islands of the Indian Ocean region. Long distance spread of CHIKV urban vectors beyond their natural flight ranges, typically a few hundred meters to a few kilometres, is usually achieved through transportation of immature stages (i.e., larvae and eggs). From the 15th century onwards, successive waves of invasion by A. aegypti and, more recently, A. albopictus, have been facilitated by commercial transport.

9Such mosquito invasions can be traced using molecular markers, which can now be developed from genome sequences. The A. aegypti genome sequence is complete and genome annotations of A. albopictus are expected soon. To assess mosquito phylogenetics, molecular markers used to define mosquito invasions have mainly been microsatellites (reviewed in) and mtDNA. Scenarios of invasions are more easily defined in island systems where mosquito populations have been geographically isolated with gene flow measurable through human displacements. The involvement of human activities in reshuffling A. albopictus distributions has been demonstrated. In Madagascar, A. albopictus dominant in the eastern coast and highland areas are genetically distant from populations from South America and Southeast Asia. This species continues to extend its geographic distribution in Madagascar, increasing its densities and progressively replacing A. aegypti, which is now present at residual levels in remote areas. The decline in A. aegypti was also detected in the neighboring island of La Réunion.

10The situation with these mosquitoes in Central Africa is slightly different. The invasion of A. albopictus occurred via several successive waves within a very short time frame. In the Central African Republic (CAR) A. albopictus first detected in 2008, probably introduced from Cameroon where the species has been established since 2000. Aedes albopictus in Cameroon derives from multiple introductions from tropical sources that still need to be identified. The polymorphism of mtDNA markers there is low, suggesting that A. albopictus were periodically introduced and that these events coincided with a decrease in A. aegypti densities.

3.3. Vector Competence

11Vector competence, the ability of an arbovirus vector to acquire a pathogen and successfully transmit it to another susceptible host, is a complex process influenced by external factors including temperature, the availability of vertebrate hosts, vector population density and predation, as well as internal factors including mosquito survival and virus replication. For CHIKV, horizontal transmission through saliva that is injected when a mosquito probes or feeds on blood is the most common mechanism of transmission (Figure 2), although vertical transmission via infected eggs may also occur at a low rate. Vector competence is typically evaluated experimentally using static laboratory-based methods, where assessment of virus replication is the primary endpoint. For CHIKV, mosquitoes from endemic locations are usually presented artificial bloodmeals loaded with known titers of virus from sympatric settings. Mosquitoes that imbibe infectious bloodmeals are incubated at a constant temperature for at least seven days and then killed to assess replication. The fractions of mosquitoes that become infected, develop infections that have disseminated from the initial infection site in the midgut into the hemocoel, and expectorate CHIKV in saliva are then measured to extrapolate vector competence for the population.

12Table 1 summarizes experimental CHIKV vector competence estimates in arthropods from around the world. Most studies have focused on the two primary urban vectors, A. aegypti and A. albopictus, although other mosquitoes and one tick species have also been tested. Most studies have used relatively high bloodmeal titers exceeding 7 log10 plaque forming units (PFU)/mL (but that are still below peaks in infected humans) and mosquitoes were typically sampled more than seven days post-feed, experimental designs that maximize the chances of CHIKV infection and transmission. Infection, dissemination, and transmission rates of A. aegypti and A. albopictus vary according to the geographic sources of mosquitoes tested. At bloodmeal titers of ≥7 log10 plaque-forming units (PFU)/mL, at least 80% of A. aegypti from all locations develop disseminated infections. More than half of A. albopictus that ingest bloodmeals ≥7 log10 PFU/mL also became infected or develop disseminated infections, with several exceptions. Infection and dissemination rates in A. albopictus from the US are dose-dependent and increase with the titer of the ingested bloodmeal; dose response studies have not been published for mosquitoes from other regions. Aedes albopictus also exhibits strain-specific susceptibility; fewer mosquitoes from La Réunion Island, Mayotte, and colonized mosquitoes originally from Texas infected in parallel with a CHIKV isolate from early in the 2005–6 La Réunion Island outbreak developed disseminated infections than mosquitoes infected with strains isolated later in the outbreak (subsequent studies discussed below revealed that A to V substitution in amino acid 226 of the E1 protein (E1-A226V) mediates much of this differential infectivity). In an attempt to exhaustively present CHIKV vector competence data, Table 1 shows all published data; however, comparing CHIKV vector competence over time in mosquitoes sampled from the same areas entails comparing data from experiments with unmatched bloodmeal titers. Differences could result from varied ingested doses rather than real differences in susceptibility to infection, dissemination and transmission. Despite this, studies from many locations worldwide show that A. aegypti and A. albopictus are generally highly susceptible to infection, dissemination and transmission of CHIKV. Alternate vector species, also represented in Table 1, may also serve as efficient CHIKV vectors in specific geographic settings; e.g., Opifex fuscus mosquitoes from New Zealand are highly competent at transmitting CHIKV from India.

3.4. Chikungunya Virus Vector Immunity and Microbial Interactions

13While most arboviruses induce significant morbidity and/or mortality in some vertebrate hosts, infections of arthropod vectors are generally considered non-pathogenic. However, interactions between the replicating virus and the mosquito immune defense system produce an outcome that may influence subsequent viral dissemination or superinfection by other viruses (reviewed in). Considerable progress has been achieved in understanding the innate defenses of the mosquito against arboviruses. The virus most intensively studied, DENV, triggers several immune pathways in A. aegypti: the Toll, JAK-STAT, and Imd/JNK pathways. However, RNA interference (RNAi) appears to be the most significant innate antiviral immune response in mosquitoes. So far, three major types of small RNA molecules have been identified in mosquito vectors: small interfering RNA (siRNA), microRNA (miRNA) and PIWI-interacting RNA (piRNA). These molecules have distinct roles in different cellular processes and virus-host interactions. The antiviral siRNA pathway is triggered when long double-stranded RNA molecules are produced from secondary RNA structures and/or viral replication intermediates during infection of the vector. This leads to an activation of the RNA degradation machinery to the target viral RNA. These pathways may act in a virus-specific manner.

14Little is known about immune responses induced by CHIKV infection of mosquitoes. Viral replication is controlled via the exogenous RNAi pathway in mosquitoes. The protein Ago-2 plays an important role in the antiviral RNAi response to CHIKV, similar to its role for other alphaviruses including, Sindbis (SINV), Semliki Forest and o’nyong-nyong viruses. Viral replication under RNAi control may limit potential pathologic effects to favor mosquito survival.

15The repeated use of insecticides to target mosquitoes as a means to control vector-borne diseases has found its limits due to the development of resistance, and alternative approaches are urgently needed. Recent strategies for controlling viral transmission have come from research on RNAi, such as the development of A. aegypti mosquitoes expressing small RNAs that render them resistant to viral infection; this approach is a promising mechanism for suppressing virus replication in mosquitoes. Depending on the objectives, mosquito populations can also be reduced in density using various strategies including the Sterile Insect Technique (SIT), a SIT-like system called Release of Insects carrying a Dominant Lethal (RIDL), or replaced by transmission-refractory mosquitoes. For this latter strategy, Aedes mosquitoes have been successfully transformed using transposon vectors or infections with the intracellular bacterium Wolbachia. However, a system is required to spread the refractory genes into mosquito populations as well as to maintain the expression of the refractory phenotype through generations. Several gene-driver systems have been proposed including the Medea system, which shows promise by coupling genes conferring disease refractoriness with a genetic mechanism for driving them through wild populations.

16In addition to their ability to shorten mosquito life span, certain strains of Wolbachia are also able to reduce arboviral transmission. Wolbachia induce various distortions of host reproduction via a form of sterility known as cytoplasmic incompatibility (CI), thereby promoting its spread into populations. Therefore, certain strains of Wolbachia provide the double role of a gene driver system and carrier of a refractory phenotype. While the mosquito A. aegypti is free of natural endosymbiotic bacteria Wolbachia, some A. albopictus populations are naturally super-infected with two Wolbachia strains, wAlbA and wAlbB. These Wolbachia, present in mosquito midguts and salivary glands, do not affect CHIKV replication. In contrast, Wolbachia are able to limit DENV in A. albopictus but cannot completely block transmission. Moreover, A. albopictus transfected with a heterologous Wolbachia isolated from the fruit fly Drosophila melanogaster (wMel) inhibit the transmission of CHIKV as in A. aegypti. Several mechanisms have been proposed to explain the molecular basis of the pathogen-blocking Wolbachia infection phenotype: upregulation of immune genes, production of reactive oxygen species, or competition for a limited resource such as cholesterol.

17In their digestive tracts, wild populations of A. albopictus and A. aegypti have been shown to house Proteobacteria and Firmicutes, including the genera Acinetobacter, Asaia, Delftia, Pseudomonas, Wolbachia and Bacillus as well as members of the family Enterobacteriaceae. A higher diversity of bacteria can be occasionally found in wild A. albopictus; 27 genera of cultivable bacteria have been detected in this species from Madagascar, with Pantea bacterium as the most prevalent. Other bacterial members of the Alpha- and Gammaproteobacteria phyla, as well as Bacteroidetes, respond to CHIKV infection. The abundance of bacteria belonging to the Enterobacteriaceae family increases with CHIKV infection, suggesting that cooperation or competition occurs within the host. Microbiota (including Wolbachia) endogenous to some mosquito species pre-activate the expression of basal genes in the immune response (i.e., immune priming), allowing the vector to be prepared for infection by pathogens. In A. aegypti infected with DENV, the arthropod microbiota elicit basal immune responses that act against the virus, and this response reduces the density of the microbial load in the mosquito midgut. Antiviral activities can be induced by secreting antiviral compounds; Serratia odorifera in A. aegypti enhances susceptibility to CHIKV by interaction of its P40 protein with the mitochondrial protein porin present on the midgut brush border membrane of the mosquito midgut, thereby downregulating mosquito immune responses.

3.5. Vector co-Infection by Chikungunya and Other Arboviruses

18Mosquitoes may feed several times during their lifespan and can ingest genetically distinct variants of the same virus species or even viruses from different families. Once a virus has infected the mosquito salivary glands, the mosquito becomes competent for transmission to the next vertebrate host, usually for the remainder of its life. The mosquito can therefore host a collection of diverse viruses, playing a role in selecting genotypes involved in epidemics.

19Because CHIKV circulates in DENV-endemic regions where the anthropophilic mosquitoes A. albopictus and A. aegypti can transmit both viruses, reports of co-infection in humans are increasing. Since the first observation in 1964 in South India, co-infections have been reported since the 2004 re-emergence: Sri-Lanka, India, Malaysia, Gabon, Madagascar, Singapore, and Angola. The increasing number of reports of co-infections seems to coincide with introductions of A. albopictus. In Gabon, between 2007 and 2010, 0.9% of 4287 acutely febrile patients were positive for both CHIKV and DENV, and more unexpectedly, co-infected A. albopictus were also collected. Patients were possibly co-infected with the two viruses through the bite of a single mosquito, as has been demonstrated experimentally. Considering the nearly worldwide circulation of DENV and CHIKV, co-infections may become more frequent. Concurrent infections may make diagnosis more challenging and could also result in different disease syndromes. Concurrent epidemics of yellow fever virus and CHIKV were also reported in Africa and patients infected with the two viruses were suspected. In the same way, Zika virus (ZIKV), typically transmitted in urban settings by A. aegypti, is also transmitted by A. albopictus, whose expanding distribution may favor the cocirculation of CHIKV and ZIKV.

4.1. Genetics of Vector Susceptibility and Host Range Changes

20As described above, CHIKV circulates in two distinct transmission cycles: (1) enzootic transmission among nonhuman primates and perhaps other vertebrates by arboreal Aedes spp. mosquitoes in sub-Saharan African sylvatic foci, and; (2) urban transmission among humans by A. aegypti and/or A. albopictus. Like interactions between other arboviruses and their arthropod vectors, susceptibility to CHIKV infection of mosquitoes and their ability to transmit depend on the genetics of both, and differences in these properties can affect circulation and human exposure as described above. Enzootic vector-CHIKV interactions have received little experimental study. Although population-based differences in urban vector competence may occur, these have not been addressed in enough detail to be conclusive or to begin to assess genetic components of vector susceptibility. Following the detection of the A. albopictus-adaptive A226V substitution in the E1 envelope glycoprotein during the 2005–2006 Réunion Island epidemic, the impact of CHIKV genetics on urban vector infection and transmission has received considerable study. Vector-adaptive evolution had been previously described for DENV and the alphavirus Venezuelan equine encephalitis virus (VEEV), the latter also involving a substitution in an envelope glycoprotein, in this case E2. Surprisingly, neither vector-adaptive alphavirus mutations has been shown to have much effect on infection of the previous or donor vector, A. aegypti in the case of CHIKV and Culex (Melanoconion) taeniopus in the case of VEEV, challenging the hypothesis that most host-specific viral adaptations will have tradeoffs for fitness in other hosts. Further phylogenetic/reverse genetic studies of IOL CHIKV revealed an unprecedented series of four second-step, A. albopictus-adaptive mutations, each involving E2 substitutions, and one relying also on a synergistic effect of an E3 substitution. Each of these mutations enhances initial infection of the mosquito midgut and has little or no effect on infection of A. aegypti. Furthermore, at least one combination of these independently acquired second-step mutations leads to an A. albopictus infection phenotype more efficient than that of any natural CHIKV strain studied to date, suggesting further vector-adaptive evolution and even more efficient circulation in regions where this mosquito is abundant. Structural modelling of these A. albopictus-adaptive envelope glycoprotein substitutions suggests that they alter the entry process in endosomes by affecting conformational changes required for E1 fusion with endosomes rather than directly affecting receptor binding.

4.2. Population Heterogeneity & Selection for Fittest Genomes

21Alphaviruses like CHIKV exist as heterogeneous populations of viral RNAs called mutant swarms that arise from frequent nucleotide misincorporation during replication due to the inability of the viral RNA-dependent RNA polymerase (RdRp) to error correct. Given a ≈12 kb CHIKV genome and a mutation frequency of ≈10−4, each new RNA genome possesses one mutation on average. By comparison, double-stranded DNA virus mutation rates are several log10 lower. Most mutant genomes are detrimental and removed from the swarm via purifying negative selection. By contrast, positive selection of a fit phenotype results in increased abundance of a genotype. Therefore, a dynamic mutation-selection balance determines the size and genetic diversity of a mutant swarm. Genetic diversity renders a population less prone to the consequences of negative selection pressures that target certain genotypes and renders a population more likely to contain variants with potential phenotypic advantages; these features can enhance plasticity and adaptability. A high fidelity CHIKV variant with a point mutation at amino acid position 483, a fidelity-determining locus in the RdRp, that was discovered experimentally after treatment with chemical mutagens, generates 30% fewer mutants than wild-type (wt) virus and is less fit in vectors and a mouse model. This reduced fitness may result from the less mutated population containing fewer genotypes that can resist evolutionary pressures including negative selection or population bottlenecks. Complementary studies with low fidelity variants of CHIKV generated by mutagenizing the amino acid at 483 produce more errors than wt variants and are also attenuated in mosquito cells and mice. Together these studies indicate that CHIKV maintains an intermediate mutation frequency to avoid detriments to fitness resulting from populations with too few or too many mutant genomes.

4.3. Dual Host Cycling & Chikungunya Virus Adaptation

22Strong purifying selection of alphaviruses that cycle between mosquitoes and vertebrates results in less genetic variation than predicted by their high mutation rates. Work with other alphaviruses including eastern equine encephalitis virus (EEEV), SINV, and VEEV shows that alternating between hosts poses conflicting challenges to replication that can limit adaptation to either host alone by imposing fitness costs where adaptations are antagonistic ( reviewed in). When one host is artificially removed via experimental serial passage, the limitations of these trade-offs are evident; viruses serially passaged in a single host are more adaptable. CHIKV serially passaged in vertebrate or mosquito cells exhibits higher fitness when passaged in novel cell types and also showed enhanced neutralization escape and antiviral compound resistance. These changes are accompanied by increased genetic diversity. In contrast, alternating CHIKV passage between cell types restricts fitness and increases diversity, suggesting that only mutations beneficial or neutral in both host cells are maintained and that these variants retain fitness in alternating cycling.

4.4. Viral Bottlenecks and Intrahost Diversity

23The ability to circumvent bottlenecks within and between dynamic environments including switching between vector and vertebrate hosts impacts CHIKV evolution and is important for understanding changing population dynamics that ultimately cause human disease. Bottlenecks that reduce arbovirus population size can influence viral fitness by restricting phenotypic plasticity that stems from having genetic diversity. The evolutionary theory Muller’s ratchet asserts that asexual organisms with high mutation rates and small population sizes irreversibly accumulate deleterious mutations unless compensatory mutations restore mutation-free genomes to the population. Studies with EEEV validate this concept; fitness decreases after serial bottleneck passages can be rescued by subsequent large population passages, albeit with much replication needed to overcome the ratchet. Anatomical barriers to productive alphavirus transmission by mosquito vectors are relatively well defined, although only one study has addressed CHIKV directly. As a first step, alphaviruses in a bloodmeal ingested by a mosquito must infect the midgut epithelium. Some VEEV studies suggest that only “portal” cells in the midgut epithelium are susceptible to infection; other experiments show uniform susceptibility, possibly reflecting a longer virus-vector relationship for the latter. Secondary impediments to dissemination result in failure of the virus to escape from the midgut epithelium, infect salivary glands, and escape from salivary gland cells into saliva for transmission to vertebrates. The number of barcoded VEEV variants that successfully traverse these barriers is reduced at midgut escape and salivary gland infection compared to the ingested bloodmeal, and smaller initial bloodmeal populations are more prone to reductions in variant diversity, suggesting strong genetic bottlenecks that reduce diversity coincident with population size changes. These observed changes in genetic diversity of the mutant swarm in mosquitoes contrast with observations from other mosquito-borne arboviruses isolated from nature, as well as experimental in vivo passaging studies with VEEV that show the maintenance of genetic and phenotypic stability of the consensus (average sequence). The disparity in results between these studies may be explained by the re-establishment of genetic diversity after bottlenecks via subsequent replication. Studies with CHIKV support this idea; although population diversity in the midgut and salivary glands is reduced compared to the bloodmeal input or midgut population, respectively, it recovers downstream of each barrier and the consensus sequence remains unchanged.

24Experimental studies showing that alternating hosts impose constraints on arboviruses including CHIKV indicate that inefficient transmission probably interrupts natural CHIKV cycling. However, the degree of natural extinction in vertebrate or vector hosts has not been directly addressed. Extinction in an individual mosquito-vertebrate-mosquito lineage is likely because most mosquitoes do not survive long enough in nature to feed more than once. The maintenance of consensus genetic stability in nature in the presence of intense circulation may be due to the extinction of most individual lineages, possibly via vector bottlenecks in individual mosquitoes. However, these extinctions must not be widespread enough; otherwise CHIKV cycling would be interrupted. No studies published on CHIKV or other alphaviruses have examined mutant swarms in naturally infected mosquitoes, humans, or other vertebrate hosts. Artificially generated, barcoded VEEV variants in mosquitoes that transmitted to laboratory mice were also observed in the brains of animals, suggesting that mosquito-to-vertebrate transmission of alphaviruses does not present a major bottleneck (although few (n = 3) infected mice were sampled). Virus doses expectorated by mosquitoes vary greatly, and are probably overestimated by standard laboratory salivation assays where infected mosquitoes eject more virus into tubes than in vivo. Therefore, variance in transmitted doses, especially when few particles are transmitted, likely impacts the outcome of vertebrate infection and maintenance of alphavirus cycling in natural foci. Interruptions in seasonal transmission may represent another significant bottleneck imposed on CHIKV, especially if the virus is introduced into temperate climates where vector survival and competence would decrease in colder temperatures; however this phenomenon is complex and has not been studied extensively.

4.5. Adaptive Constraints on Chikungunya Virus Evolution

25Although the 2004 IOL CHIK emergence underscores the adaptive potential of RNA viruses including most arboviruses, it also provides examples of constraints on adaptive evolution that remain difficult to predict. Although the E1-A226V substitution was selected convergently after IOL strains reached locations with abundant A. albopictus, it surprisingly was not found in any CHIKV strains of the Asian lineage despite their circulation in regions of Asia native to this vector for more than 60 years. This lack of adaptation to A. albopictus in Asia resulted from an epistatic interaction with E1 residue 98; Asian CHIKV strains, which have a threonine residue at position 98, show no increase in A. albopictus infectivity when the E1-A226V substitution is engineered into a cDNA clone derived from the Asian strain, while ECSA and IOL strains with 98A show a dramatic increase in infection. The lack of sequenced enzootic CHIKV strains with 98T suggests that this residue, which by itself has no detectable effect on infection of urban vectors or models for human infection, resulted from a founder effect when CHIKV was introduced into Asia sometime before 1958.

26Although variation among Asian strain CHIKV infection of A. aegypti has received little study, no evidence has been produced to support adaptive evolution in Asia since the 1950s. The above evidence indicating an adaptive constraint on Asian strains, including those now circulating in the Americas, for enhanced transmission by A. albopictus suggests that A. aegypti will remain the principal vectors as spread into American regions inhabited by both vectors continues. However, additional studies of the Asian lineage and its potential to increase transmissibility by either mosquito are needed.

5.1. Potential for Re-Emergence and Expansion into New Areas

27Due to the immunologically almost completely naïve status of human populations and the widespread and abundant A. aegypti nearly throughout the western hemisphere tropics, CHIKV is expected to continue to spread and ultimately recapitulate the distribution of DENV in the Americas. The lack of historical evidence for a major role of A. albopictus in transmission of Asian lineage CHIKV strains and the adaptive constraint described above suggest that temperate American regions inhabited by A. albopictus but not A. aegypti may not be at as high a level of risk as regions where IOL strains are circulating. However, IOL strains continue to circulate in Asia and have a history of many importations into the Americas, so their risk of introduction into the Americas continues. Should both Asian and IOL strains cocirculate, as they have in Southeast Asia since 2007, even more geographic regions of the Americas could be at risk for CHIK.

5.2. Prospects for Prevention via Vector Control

28The poor history of DENV control since the 1970s suggests that mitigation of CHIKV transmission via vector control will be highly challenging. Due to its tight association with artificial larval habitats, endophily of adult females, and daytime biting patterns, control of A. aegypti and DENV transmission is difficult, and A. albopictus presents similar challenges. The development of resistance to commonly used insecticides further complicates the control of these vectors. Although some novel strategies for A. aegypti control discussed above such as RIDL offer promise, they remain in the early stages of field testing. Interim measures including the application of persistent insecticides to the interior of houses may be needed in some situations to reduce CHIKV and DENV transmission.

29The long-term prospects for CHIKV maintenance in the endemic, urban cycle are not entirely clear. Following introduction of the Asian lineage into South and Southeast Asia during the 1950s or earlier, it became extinct in India after 1973 for unknown reasons, but continues to circulate in Southeast Asia today. The only major human immune cross-reactivity known to affect CHIKV to a major extent is that from Ross River and Mayaro viruses, which like CHIKV are members of the Semliki Forest complex of alphaviruses. Ross River virus only occurs in Australia and Indonesia, and Mayaro virus in South America where seroprevalence is generally low in urban settings. These data suggest that CHIKV will continue to circulate indefinitely in Asia and probably in the Americas as well.

6. Conclusions

30Chikungunya virus has caused explosive outbreaks of severe, debilitating and often chronic arthralgia since it emerged in the 1950s and later in 2004 from the enzootic ECSA lineage in Africa. The large populations of susceptible humans in many naïve regions and thriving populations of the two urban vectors, A. aegypti and A. albopictus, will probably facilitate endemicity throughout most regions of the tropics and subtropics for the foreseeable future, although the burden of disease is typically difficult to estimate because CHIKV and DENV infections are difficult to distinguish clinically. Further adaptation of CHIKV to these urban vectors is also suggested by recent findings. Future emergences from enzootic African cycles will also remain a risk that is increasing with more and more air travel and international commerce. Although novel strategies to control vector populations and reduce transmission are in early stages of field testing, the deployment of one of several highly promising human vaccines probably offers the best hope for making a major impact in restricting CHIKV circulation and preventing human disease.

References 201

1.
Powers A.M., Logue C.H. Changing patterns of chikungunya virus: Re-emergence of a zoonotic arbovirus. J. Gen. Virol. 2007;88:2363-2377.
Not in our library10.1099/vir.0.82858-0
2.
Kuehn B.M. Chikungunya virus transmission found in the United States: US health authorities brace for wider spread. JAMA. 2014;312:776-777.
Not in our library10.1001/jama.2014.9916
3.
CDC. Chikungunya virus in the United States.
Not in our library
4.
Jupp P.G., McIntosh B.M. Aedes furcifer and other mosquitoes as vectors of chikungunya virus at Mica, Northeastern Transvaal, South Africa. J. Am. Mosq. Control. Assoc. 1990;6:415-420.
Not in our library
5.
Volk S.M., Chen R., Tsetsarkin K.A., Adams A.P., Garcia T.I., Sall A.A., Nasar F., Schuh A.J., Holmes E.C., Higgs S., et al. Genome-scale phylogenetic analyses of chikungunya virus reveal independent emergences of recent epidemics and various evolutionary rates. J. Virol. 2010;84:6497-6504.
Not in our library10.1128/jvi.01603-09
6.
Tsetsarkin K.A., Chen R., Sherman M.B., Weaver S.C. Chikungunya virus: Evolution and genetic determinants of emergence. Curr. Opin. Virol. 2011;1:310-317.
Not in our library10.1016/j.coviro.2011.07.004
7.
Kariuki Njenga M., Nderitu L., Ledermann J.P., Ndirangu A., Logue C.H., Kelly C.H., Sang R., Sergon K., Breiman R., Powers A.M. Tracking epidemic chikungunya virus into the Indian Ocean from East Africa. J. Gen. Virol. 2008;89:2754-2760.
Not in our library10.1099/vir.0.2008/005413-0
8.
Genome Microevolution of Chikungunya Viruses Causing the Indian Ocean Outbreak · 2006
No notice
9.
A Single Mutation in Chikungunya Virus Affects Vector Specificity and Epidemic Potential · 2007
No notice
10.
Two Chikungunya Isolates from the Outbreak of La Reunion (Indian Ocean) Exhibit Different Patterns of Infection in the Mosquito, Aedes albopictus · 2007
No notice
11.
Epistatic Roles of E2 Glycoprotein Mutations in Adaption of Chikungunya Virus to Aedes Albopictus and Ae. Aegypti Mosquitoes · 2009
No notice
12.
Arias-Goeta C., Mousson L., Rougeon F., Failloux A.B. Dissemination and transmission of the E1–226V variant of chikungunya virus in Aedes albopictus are controlled at the midgut barrier level. PLoS One. 2013;8:e57548.
Not in our library10.1371/journal.pone.0057548
13.
Tsetsarkin K.A., Chen R., Yun R., Rossi S.L., Plante K.S., Guerbois M., Forrester N., Perng G.C., Sreekumar E., Leal G., et al. Multi-peaked adaptive landscape for chikungunya virus evolution predicts continued fitness optimization in Aedes albopictus mosquitoes. Nat. Commun. 2014;5:4084.
Not in our library10.1038/ncomms5084
14.
Outbreak of Dengue and Chikungunya Fevers, Toamasina, Madagascar, 2006 · 2008
No notice
15.
Chikungunya virus adapts to tiger mosquito via evolutionary convergence: a sign of things to come? · 2008
No notice
16.
Bagny L., Delatte H., Quilici S., Fontenille D. Progressive decrease in Aedes aegypti distribution in reunion island since the 1900s. J. Med. Entomol. 2009;46:1541-1545.
Not in our library10.1603/033.046.0644
17.
Kumar N.P., Joseph R., Kamaraj T., Jambulingam P. A226V mutation in virus during the 2007 chikungunya outbreak in Kerala, India. J. Gen. Virol. 2008;89:1945-1948.
Not in our library10.1099/vir.0.83628-0
18.
Chikungunya Outbreaks Caused by African Genotype, India · 2006
No notice
19.
Kularatne S.A., Gihan M.C., Weerasinghe S.C., Gunasena S. Concurrent outbreaks of chikungunya and dengue fever in Kandy, Sri Lanka, 2006–07: A comparative analysis of clinical and laboratory features. Postgrad. Med. J. 2009;85:342-346.
Not in our library10.1136/pgmj.2007.066746
20.
Chikungunya Virus, Cameroon, 2006 · 2007
No notice
21.
Peyrefitte C.N., Bessaud M., Pastorino B.A., Gravier P., Plumet S., Merle O.L., Moltini I., Coppin E., Tock F., Daries W., et al. Circulation of chikungunya virus in Gabon, 2006–2007. J. Med. Virol. 2008;80:430-433.
Not in our library10.1002/jmv.21090
22.
Paupy C., Kassa Kassa F., Caron M., Nkoghe D., Leroy E.M. A chikungunya outbreak associated with the vector Aedes albopictus in remote villages of Gabon. Vector Borne Zoonotic Dis. 2012;12:167-169.
Not in our library10.1089/vbz.2011.0736
23.
Mombouli J.V., Bitsindou P., Elion D.O., Grolla A., Feldmann H., Niama F.R., Parra H.J., Munster V.J. Chikungunya virus infection, Brazzaville, Republic of Congo, 2011. Emerg. Infect. Dis. 2013;19:1542-1543.
Not in our library10.3201/eid1909.130451
24.
Rezza G., Nicoletti L., Angelini R., Romi R., Finarelli A.C., Panning M., Cordioli P., Fortuna C., Boros S., Magurano F., et al. Infection with chikungunya virus in Italy: An outbreak in a temperate region. Lancet. 2007;370:1840-1846.
Not in our library10.1016/s0140-6736(07)61779-6
25.
Sam I.C., Chan Y.F., Chan S.Y., Loong S.K., Chin H.K., Hooi P.S., Ganeswrie R., Abubakar S. Chikungunya virus of Asian and Central/East African genotypes in Malaysia. J. Clin. Virol. 2009;46:180-183.
Not in our library10.1016/j.jcv.2009.07.016
26.
Ng L.C., Tan L.K., Tan C.H., Tan S.S., Hapuarachchi H.C., Pok K.Y., Lai Y.L., Lam-Phua S.G., Bucht G., Lin R.T., et al. Entomologic and virologic investigation of chikungunya, Singapore. Emerg. Infect. Dis. 2009;15:1243-1249.
Not in our library10.3201/eid1508.081486
27.
Theamboonlers A., Rianthavorn P., Praianantathavorn K., Wuttirattanakowit N., Poovorawan Y. Clinical and molecular characterization of chikungunya virus in South Thailand. Jpn. J. Infect. Dis. 2009;62:303-305.
Not in our library
28.
Wu D., Wu J., Zhang Q., Zhong H., Ke C., Deng X., Guan D., Li H., Zhang Y., Zhou H., et al. Chikungunya outbreak in Guangdong Province, China, 2010. Emerg. Infect. Dis. 2012;18:493-495.
Not in our library10.3201/eid1803.110034
29.
Duong V., Andries A.C., Ngan C., Sok T., Richner B., Asgari-Jirhandeh N., Bjorge S., Huy R., Ly S., Laurent D., et al. Reemergence of chikungunya virus in Cambodia. Emerg. Infect. Dis. 2012;18:2066-2069.
Not in our library10.3201/eid1812.120471
30.
Wangchuk S., Chinnawirotpisan P., Dorji T., Tobgay T., Dorji T., Yoon I.K., Fernandez S. Chikungunya fever outbreak, Bhutan, 2012. Emerg. Infect. Dis. 2013;19:1681-1684.
Not in our library10.3201/eid1910.130453
31.
Hapuarachchi H.C., Bandara K.B., Sumanadasa S.D., Hapugoda M.D., Lai Y.L., Lee K.S., Tan L.K., Lin R.T., Ng L.F., Bucht G., et al. Re-emergence of chikungunya virus in south-east Asia: Virological evidence from Sri Lanka and Singapore. J. Gen. Virol. 2010;91:1067-1076.
Not in our library10.1099/vir.0.015743-0
32.
Rianthavorn P., Prianantathavorn K., Wuttirattanakowit N., Theamboonlers A., Poovorawan Y. An outbreak of chikungunya in Southern Thailand from 2008 to 2009 caused by African strains with A226V mutation. Int. J. Infect. Dis. 2010;14:e161-e165.
Not in our library10.1016/j.ijid.2010.01.001
33.
Gould E.A., Gallian P., De Lamballerie X., Charrel R.N. First cases of autochthonous dengue fever and chikungunya fever in France: From bad dream to reality! Clin. Microbiol. Infect. 2010;16:1702-1704.
Not in our library10.1111/j.1469-0691.2010.03386.x
34.
Chikungunya Virus, Southeastern France · 2011
No notice
35.
Delaunay P., Jeannin C., Schaffner F., Marty P. News on the presence of the tiger mosquito Aedes albopictus in metropolitan France. Arch. Pediatr. 2009;16:S66-S71.
Not in our library10.1016/s0929-693x(09)75304-7
36.
High Efficiency of Temperate Aedes albopictus to Transmit Chikungunya and Dengue Viruses in the Southeast of France · 2013
No notice
37.
Paty M., Six C., Charlet F., Heuze G., Cochet A., Wiegandt A., Chappert J., Dejour-Salamanca D., Guinard A., Soler P., et al. Large number of imported chikungunya cases in mainland France, 2014: A challenge for surveillance and response. Euro Surveill. 2014;19:20856.
Not in our library10.2807/1560-7917.es2014.19.28.20856
38.
Tomasello D., Schlagenhauf P. Chikungunya and dengue autochthonous cases in Europe, 2007–2012. Travel Med. Infect. Dis. 2013;11:274-284.
Not in our library10.1016/j.tmaid.2013.07.006
39.
Napoli C., Salcuni P., Pompa M.G., Declich S., Rizzo C. Estimated imported infections of chikungunya and dengue in Italy, 2008 to 2011. J. Travel Med. 2012;19:294-297.
Not in our library10.1111/j.1708-8305.2012.00640.x
40.
Leparc-Goffart I., Nougairede A., Cassadou S., Prat C., de Lamballerie X. Chikungunya in the Americas. Lancet. 2014;383:514.
Not in our library10.1016/s0140-6736(14)60185-9
41.
Dupont-Rouzeyrol M., Caro V., Guillaumot L., Vazeille M., DʼOrtenzio E., Thiberge J.M., Baroux N., Gourinat A.C., Grandadam M., Failloux A.B. Chikungunya virus and the mosquito vector Aedes aegypti in New Caledonia (South Pacific region). Vector borne and Zoonotic Dis. 2012;12:1036-1041.
Not in our library10.1089/vbz.2011.0937
42.
Arrival of Chikungunya Virus in the New World: Prospects for Spread and Impact on Public Health · 2014
No notice
43.
Vega-Rua A., Zouache K., Girod R., Failloux A.B., Lourenco-de-Oliveira R. High level of vector competence of Aedes aegypti and Aedes albopictus from ten American countries as a crucial factor in the spread of chikungunya virus. J. Virol. 2014;88:6294-6306.
Not in our library10.1128/jvi.00370-14
44.
ARBOnet. Chikungunya Virus Disease—United States, 2014 Provisional Data Reported to Arbonet. 2014.
Not in our library
45.
Weinbren M.P., Haddow A.J., Williams M.C. The occurrence of chikungunya virus in Uganda. I. Isolation from mosquitoes. Trans. R. Soc. Trop. Med. Hygiene. 1958;52:253-257.
Not in our library10.1016/0035-9203(58)90084-1
46.
McCarthy M.C., Haberberger R.L., Salib A.W., Soliman B.A., El-Tigani A., Khalid I.O., Watts D.M. Evaluation of arthropod-borne viruses and other infectious disease pathogens as the causes of febrile illnesses in the Khartoum Province of Sudan. J. Med. Virol. 1996;48:141-146.
Not in our library10.1002/(sici)1096-9071(199602)48:2<141::aid-jmv4>3.0.co;2-9
47.
Diallo M., Thonnon J., Traore-Lamizana M., Fontenille D. Vectors of chikungunya virus in Senegal: Current data and transmission cycles. The American journal of tropical medicine and hygiene. 1999;60:281-286.
Not in our library10.4269/ajtmh.1999.60.281
48.
Jupp P.G., Kemp A. What is the potential for future outbreaks of chikungunya, dengue and yellow fever in Southern Africa? South. Afr. Med. J. 1996;86:35-37.
Not in our library
49.
Gubler D.J. Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century. Trends Microbiol. 2002;10:100-103.
Not in our library10.1016/s0966-842x(01)02288-0
50.
McClelland G.A.H. A worldwide survey of variation in scale pattern of the abdominal tergum of Aedes aegypti (l.) (diptera: Culicidae). Trans. R. Entomol. Soc. Lond. 1974;126:239-259.
Not in our library10.1111/j.1365-2311.1974.tb00853.x
51.
Tabachnick W.J., Powell J.R. A world-wide survey of genetic variation in the yellow fever mosquito, Aedes Aegypti. Genet. Res. 1979;34:215-229.
Not in our library10.1017/s0016672300019467
52.
Failloux A.B., Vazeille M., Rodhain F. Geographic genetic variation in populations of the dengue virus vector Aedes aegypti. J. Mol. Evolut. 2002;55:653-663.
Not in our library10.1007/s00239-002-2360-y
53.
Powell J.R., Tabachnick W.J. History of domestication and spread of Aedes aegypti—A review. Memorias do Instituto Oswaldo Cruz. 2013;108:11-17.
Not in our library10.1590/0074-0276130395
54.
Juliano S.A., Lounibos L.P., OʼMeara G.F. A field test for competitive effects of Aedes albopictus on Aedes aegypti in South Florida: Differences between sites of coexistence and exclusion? Oecologia. 2004;139:583-593.
Not in our library10.1007/s00442-004-1532-4
55.
Braks M.A., Honorio N.A., Lourencqo-De-Oliveira R., Juliano S.A., Lounibos L.P. Convergent habitat segregation of Aedes aegypti and Aedes albopictus (diptera: Culicidae) in Southeastern Brazil and Florida. J. Med. Entomol. 2003;40:785-794.
Not in our library10.1603/0022-2585-40.6.785
56.
Simard F., Nchoutpouen E., Toto J.C., Fontenille D. Geographic distribution and breeding site preference of Aedes albopictus and Aedes aegypti (diptera: Culicidae) in Cameroon, Central Africa. J. Med. Entomol. 2005;42:726-731.
Not in our library10.1603/0022-2585(2005)042[0726:gdabsp]2.0.co;2
57.
Smith C.E. The history of dengue in tropical Asia and its probable relationship to the mosquito Aedes aegypti. J. Trop. Med. Hygiene. 1956;59:243-251.
Not in our library
58.
Medlock J.M., Hansford K.M., Schaffner F., Versteirt V., Hendrickx G., Zeller H., Van Bortel W. A review of the invasive mosquitoes in Europe: Ecology, public health risks, and control options. Vector borne Zoonotic Dis. 2012;12:435-447.
Not in our library10.1089/vbz.2011.0814
59.
Lounibos L.P. Invasions by insect vectors of human disease. Annu. Rev. Entomol. 2002;47:233-266.
Not in our library10.1146/annurev.ento.47.091201.145206
60.
Rai K.S. Aedes albopictus in the Americas. Annu. Rev. Entomol. 1991;36:459-484.
Not in our library10.1146/annurev.en.36.010191.002331
61.
Hawley W.A., Reiter P., Copeland R.S., Pumpuni C.B., Craig G.B. Aedes albopictus in North America: Probable introduction in used tires from Northern Asia. Science. 1987;236:1114-1116.
Not in our library10.1126/science.3576225
62.
Sprenger D., Wuithiranyagool T. The discovery and distribution of Aedes albopictus in Harris County, Texas. J. Am. Mosq. Control Assoc. 1986;2:217-219.
Not in our library
63.
Adhami J., Reiter P. Introduction and establishment of Aedes (Stegomyia) albopictus skuse (diptera: Culicidae) in Albania. J. Am. Mosq. Control Assoc. 1998;14:340-343.
Not in our library
64.
Sabatini A., Raineri V., Trovato G., Coluzzi M. Aedes albopictus in Italy and possible diffusion of the species into the Mediterranean area. Parassitologia. 1990;32:301-304.
Not in our library
65.
Hawley W.A. The biology of Aedes albopictus. J. Am. Mosq. Control Association. Suppl. 1988;1:1-39.
Not in our library
66.
Paupy C., Delatte H., Bagny L., Corbel V., Fontenille D. Aedes albopictus, an arbovirus vector: From the darkness to the light. Microbes Infect. 2009;11:1177-1185.
Not in our library10.1016/j.micinf.2009.05.005
67.
Metselaar D., Grainger C.R., Oei K.G., Reynolds D.G., Pudney M., Leake C.J., Tukei P.M., D’Offay R.M., Simpson D.I. An outbreak of type 2 dengue fever in the Seychelles, probably transmitted by Aedes albopictus (skuse). Bull. World Health Organ. 1980;58:937-943.
Not in our library
68.
Delatte H., Dehecq J.S., Thiria J., Domerg C., Paupy C., Fontenille D. Geographic distribution and developmental sites of Aedes albopictus (diptera: Culicidae) during a chikungunya epidemic event. Vector Borne Zoonotic Dis. 2008;8:25-34.
Not in our library10.1089/vbz.2007.0649
69.
Gjenero-Margan I., Aleraj B., Krajcar D., Lesnikar V., Klobucar A., Pem-Novosel I., Kurecic-Filipovic S., Komparak S., Martic R., Duricic S., et al. Autochthonous dengue fever in Croatia, August-September 2010. Euro Surveillance. 2011;16:9.
Not in our library
70.
La Ruche G., Souares Y., Armengaud A., Peloux-Petiot F., Delaunay P., Despres P., Lenglet A., Jourdain F., Leparc-Goffart I., Charlet F., et al. First two autochthonous dengue virus infections in metropolitan France, September 2010. Euro Surveill. 2010;15:19676.
Not in our library
71.
Juliano S.A., O'Meara G.F., Morrill J.R., Cutwa M.M. Desiccation and thermal tolerance of eggs and the coexistence of competing mosquitoes. Oecologia. 2002;130:458-469.
Not in our library10.1007/s004420100811
72.
O’Meara G.F., Evans L.F., Gettman A.D., Cuda J.P. Spread of Aedes albopictus and decline of Ae. aegypti (diptera: Culicidae) in Florida. J. Med. Entomol. 1995;32:554-562.
Not in our library10.1093/jmedent/32.4.554
73.
Juliano S.A., Lounibos L.P. Ecology of invasive mosquitoes: Effects on resident species and on human health. Ecol. Lett. 2005;8:558-574.
Not in our library10.1111/j.1461-0248.2005.00755
74.
Kamgang B., Brengues C., Fontenille D., Njiokou F., Simard F., Paupy C. Genetic structure of the tiger mosquito, Aedes albopictus, in Cameroon (Central Africa). PLoS One. 2011;6:e20257.
Not in our library10.1371/journal.pone.0020257
75.
Kamgang B., Ngoagouni C., Manirakiza A., Nakoune E., Paupy C., Kazanji M. Temporal patterns of abundance of Aedes aegypti and Aedes albopictus (diptera: Culicidae) and mitochondrial DNA analysis of Ae. albopictus in the Central African Republic. PLoS Negl. Trop. Dis. 2013;7:e2590.
Not in our library10.1371/journal.pntd.0002590
76.
Raharimalala F.N., Ravaomanarivo L.H., Ravelonandro P., Rafarasoa L.S., Zouache K., Tran-Van V., Mousson L., Failloux A.B., Hellard E., Moro C.V., et al. Biogeography of the two major arbovirus mosquito vectors, Aedes aegypti and Aedes albopictus (diptera, culicidae), in Madagascar. Parasites Vectors. 2012;5:56.
Not in our library10.1186/1756-3305-5-56
77.
Reiter P., Sprenger D. The used tire trade: A mechanism for the worldwide dispersal of container breeding mosquitoes. J. Am. Mosq. Control Assoc. 1987;3:494-501.
Not in our library
78.
Nene V., Wortman J.R., Lawson D., Haas B., Kodira C., Tu Z.J., Loftus B., Xi Z., Megy K., Grabherr M., et al. Genome sequence of Aedes aegypti, a major arbovirus vector. Science. 2007;316:1718-1723.
Not in our library10.1126/science.1138878
79.
Bonizzoni M., Gasperi G., Chen X., James A.A. The invasive mosquito species Aedes albopictus: Current knowledge and future perspectives. Trends Parasitol. 2013;29:460-468.
Not in our library10.1016/j.pt.2013.07.003
80.
Urdaneta-Marquez L., Failloux A.B. Population genetic structure of Aedes aegypti, the principal vector of dengue viruses. Infect. Genetics Evol. 2011;11:253-261.
Not in our library10.1016/j.meegid.2010.11.020
81.
Birungi J., Munstermann L.E. Genetic structure of Aedes albopictus (diptera: Culicidae) populations based on mitochondrial nd5 sequences: Evidence for an independent invasion into Brazil and United States. Ann. Entomol. Soc. Am. 2002;95:125-132.
Not in our library10.1603/0013-8746(2002)095[0125:gsoaad]2.0.co;2
82.
Maia R.T., Scarpassa V.M., Maciel-Litaiff L.H., Tadei W.P. Reduced levels of genetic variation in Aedes albopictus (diptera: Culicidae) from Manaus, Amazonas State, Brazil, based on analysis of the mitochondrial DNA nd5 gene. Genetics Mol. Res. 2009;8:998-1007.
Not in our library10.4238/vol8-3gmr624
83.
Mousson L., Dauga C., Garrigues T., Schaffner F., Vazeille M., Failloux A.B. Phylogeography of Aedes (stegomyia) aegypti (l.) and Aedes (stegomyia) albopictus (skuse) (diptera: Culicidae) based on mitochondrial DNA variations. Genet. Res. 2005;86:1-11.
Not in our library10.1017/s0016672305007627
84.
Usmani-Brown S., Cohnstaedt L., Munstermann L.E. Population genetics of Aedes albopictus (diptera: Culicidae) invading populations, using mitochondrial nicotinamide adenine dinucleotide dehydrogenase subunit 5 sequences. Ann. Entomol. Soc. Am. 2009;102:144-150.
Not in our library10.1603/008.102.0116
85.
Gillespie R.G., Roderick G.K. Arthropods on islands: Colonization, speciation, and conservation. Annu. Rev. Entomol. 2002;47:595-632.
Not in our library10.1146/annurev.ento.47.091201.145244
86.
Beebe N.W., Ambrose L., Hill L.A., Davis J.B., Hapgood G., Cooper R.D., Russell R.C., Ritchie S.A., Reimer L.J., Lobo N.F., et al. Tracing the tiger: Population genetics provides valuable insights into the Aedes (stegomyia) albopictus invasion of the Australasian region. PLoS Negl. Trop. Dis. 2013;7:e2361.
Not in our library10.1371/journal.pntd.0002361
87.
Fontenille D., Rodhain F. Biology and distribution of Aedes albopictus and Aedes aegypti in Madagascar. J. Am. Mosq. Control Assoc. 1989;5:219-225.
Not in our library
88.
Bagny L., Arnoux S., Delatte H., Lajoie G., Fontenille D. Spread of invasive Aedes albopictus and decline of resident Aedes aegypti in urban areas of Mayotte 2007–2010. Biol. Invasions. 2012;14:1623-1633.
Not in our library10.1007/s10530-012-0177-1
89.
Diallo M., Laganier R., Nangouma A. First record of Ae. albopictus (skuse 1894), in Central African Republic. Trop. Med. Int. Health. 2010;15:1185-1189.
Not in our library10.1111/j.1365-3156.2010.02594.x
90.
Fontenille D., Toto J.C. Aedes (stegomyia) albopictus (skuse), a potential new dengue vector in Southern Cameroon. Emerg. Infect. Dis. 2001;7:1066-1067.
Not in our library10.3201/eid0706.010631
91.
Agarwal A., Dash P.K., Singh A.K., Sharma S., Gopalan N., Rao P.V., Parida M.M., Reiter P. Evidence of experimental vertical transmission of emerging novel ECSA genotype of chikungunya virus in Aedes aegypti. PLoS Negl. Trop. Dis. 2014;8:e2990.
Not in our library10.1371/journal.pntd.0002990
92.
Chikungunya Fever: A Clinical and Virological Investigation of Outpatients on Reunion Island, South-West Indian Ocean · 2013
No notice
93.
Pesko K., Westbrook C.J., Mores C.N., Lounibos L.P., Reiskind M.H. Effects of infectious virus dose and bloodmeal delivery method on susceptibility of Aedes aegypti and Aedes albopictus to chikungunya virus. J. Med. Entomol. 2009;46:395-399.
Not in our library10.1603/033.046.0228
94.
Differential responses of the mosquito <it>Aedes albopictus </it>from the Indian Ocean region to two chikungunya isolates · 2010
Not checked
95.
Sanchez-Vargas I., Travanty E.A., Keene K.M., Franz A.W., Beaty B.J., Blair C.D., Olson K.E. RNA interference, arthropod-borne viruses, and mosquitoes. Virus Res. 2004;102:65-74.
Not in our library10.1016/j.virusres.2004.01.017
96.
The Aedes aegypti Toll Pathway Controls Dengue Virus Infection · 2008
No notice
97.
Ramirez J.L., Dimopoulos G. The toll immune signaling pathway control conserved anti-dengue defenses across diverse Ae. aegypti strains and against multiple dengue virus serotypes. Dev. Compar. Immunol. 2010;34:625-629.
Not in our library10.1016/j.dci.2010.01.006
98.
Souza-Neto J.A., Sim S., Dimopoulos G. An evolutionary conserved function of the jak-stat pathway in anti-dengue defense. Proc. Natl. Acad. Sci. USA. 2009;106:17841-17846.
Not in our library10.1073/pnas.0905006106
99.
Sanders H.R., Foy B.D., Evans A.M., Ross L.S., Beaty B.J., Olson K.E., Gill S.S. Sindbis virus induces transport processes and alters expression of innate immunity pathway genes in the midgut of the disease vector, Aedes aegypti. Insect Biochem. Mol. Biol. 2005;35:1293-1307.
Not in our library10.1016/j.ibmb.2005.07.006
100.
Dengue Virus Type 2 Infections of Aedes aegypti Are Modulated by the Mosquito's RNA Interference Pathway · 2009
No notice
101.
Keene K.M., Foy B.D., Sanchez-Vargas I., Beaty B.J., Blair C.D., Olson K.E. RNA interference acts as a natural antiviral response to o’nyong-nyong virus (alphavirus; Togaviridae) infection of Anopheles gambiae. Proc. Natl. Acad. Sci. USA. 2004;101:17240-17245.
Not in our library10.1073/pnas.0406983101
102.
Aedes aegyptiuses RNA interference in defense against Sindbis virus infection · 2008
No notice
103.
Myles K.M., Wiley M.R., Morazzani E.M., Adelman Z.N. Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes. Proc. Natl. Acad. Sci. USA. 2008;105:19938-19943.
Not in our library10.1073/pnas.0803408105
104.
Cirimotich C.M., Scott J.C., Phillips A.T., Geiss B.J., Olson K.E. Suppression of RNA interference increases alphavirus replication and virus-associated mortality in Aedes aegypti mosquitoes. BMC Microbiol. 2009;9:49.
Not in our library10.1186/1471-2180-9-49
105.
The RNA interference pathway affects midgut infection- and escape barriers for Sindbis virus in <it>Aedes aegypti</it> · 2010
Not checked
106.
Donald C.L., Kohl A., Schnettler E. New insights into control of arbovirus replication and spread by insect RNA interference pathways. Insects. 2012;3:511-531.
Not in our library10.3390/insects3020511
107.
McFarlane M., Arias-Goeta C., Martin E., OʼHara Z., Lulla A., Mousson L., Rainey S.M., Misbah S., Schnettler E., Donald C.L., et al. Characterization of Aedes aegypti innate-immune pathways that limit chikungunya virus replication. PLoS Negl. Trop. Dis. 2014;8:e2994.
Not in our library10.1371/journal.pntd.0002994
108.
Knockdown of piRNA pathway proteins results in enhanced Semliki Forest virus production in mosquito cells · 2013
No notice
109.
McGraw E.A., OʼNeill S.L. Beyond insecticides: New thinking on an ancient problem. Nat. Rev. Microbiol. 2013;11:181-193.
Not in our library10.1038/nrmicro2968
110.
Franz A.W., Sanchez-Vargas I., Adelman Z.N., Blair C.D., Beaty B.J., James A.A., Olson K.E. Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti. Proc. Natl. Acad. Sci. USA. 2006;103:4198-4203.
Not in our library10.1073/pnas.0600479103
111.
Mathur G., Sanchez-Vargas I., Alvarez D., Olson K.E., Marinotti O., James A.A. Transgene-mediated suppression of dengue viruses in the salivary glands of the yellow fever mosquito, Aedes aegypti. Insect Mol. Biol. 2010;19:753-763.
Not in our library10.1111/j.1365-2583.2010.01032.x
112.
Travanty E.A., Adelman Z.N., Franz A.W., Keene K.M., Beaty B.J., Blair C.D., James A.A., Olson K.E. Using RNA interference to develop dengue virus resistance in genetically modified Aedes aegypti. Insect Biochem. Mol. Biol. 2004;34:607-613.
Not in our library10.1016/j.ibmb.2004.03.013
113.
Alphey L., McKemey A., Nimmo D., Neira Oviedo M., Lacroix R., Matzen K., Beech C. Genetic control of Aedes mosquitoes. Pathog. Glob. Health. 2013;107:170-179.
Not in our library10.1179/2047773213y.0000000095
114.
Alphey L. Natural and engineered mosquito immunity. J. Biol. 2009;8:40.
Not in our library10.1186/jbiol143
115.
Fraser M.J. Insect transgenesis: Current applications and future prospects. Annu. Rev. Entomol. 2012;57:267-289.
Not in our library10.1146/annurev.ento.54.110807.090545
116.
Walker T., Johnson P.H., Moreira L.A., Iturbe-Ormaetxe I., Frentiu F.D., McMeniman C.J., Leong Y.S., Dong Y., Axford J., Kriesner P., et al. The wMel wolbachia strain blocks dengue and invades caged Aedes aegypti populations. Nature. 2011;476:450-453.
Not in our library10.1038/nature10355
117.
Sinkins S.P., Gould F. Gene drive systems for insect disease vectors. Nat. Rev. Genet. 2006;7:427-435.
Not in our library10.1038/nrg1870
118.
Chen C.H., Huang H., Ward C.M., Su J.T., Schaeffer L.V., Guo M., Hay B.A. A synthetic maternal-effect selfish genetic element drives population replacement in Drosophila. Science. 2007;316:597-600.
Not in our library10.1126/science.1138595
119.
McMeniman C.J., Lane R.V., Cass B.N., Fong A.W., Sidhu M., Wang Y.F., OʼNeill S.L. Stable introduction of a life-shortening wolbachia infection into the mosquito Aedes aegypti. Science. 2009;323:141-144.
Not in our library10.1126/science.1165326
120.
Hedges L.M., Brownlie J.C., O’Neill S.L., Johnson K.N. Wolbachia and virus protection in insects. Science. 2008;322:702.
Not in our library10.1126/science.1162418
121.
Moreira L.A., Iturbe-Ormaetxe I., Jeffery J.A., Lu G., Pyke A.T., Hedges L.M., Rocha B.C., Hall-Mendelin S., Day A., Riegler M., et al. A wolbachia symbiont in Aedes aegypti limits infection with dengue, chikungunya, and plasmodium. Cell. 2009;139:1268-1278.
Not in our library10.1016/j.cell.2009.11.042
122.
Hancock P.A., Sinkins S.P., Godfray H.C. Strategies for introducing wolbachia to reduce transmission of mosquito-borne diseases. PLoS Negl. Trop. Dis. 2011;5:e1024.
Not in our library10.1371/journal.pntd.0001024
123.
Zouache K., Voronin D., Tran-Van V., Mousson L., Failloux A.B., Mavingui P. Persistent wolbachia and cultivable bacteria infection in the reproductive and somatic tissues of the mosquito vector Aedes albopictus. PLoS One. 2009;4:e6388.
Not in our library10.1371/journal.pone.0006388
124.
Mousson L., Martin E., Zouache K., Madec Y., Mavingui P., Failloux A.B. Wolbachia modulates chikungunya replication in Aedes albopictus. Mol. Ecol. 2010;19:1953-1964.
Not in our library10.1111/j.1365-294x.2010.04606.x
125.
The Native Wolbachia Symbionts Limit Transmission of Dengue Virus in Aedes albopictus · 2012
No notice
126.
Wolbachia Induces Density-Dependent Inhibition to Dengue Virus in Mosquito Cells · 2012
No notice
127.
Blagrove M.S., Arias-Goeta C., Di Genua C., Failloux A.B., Sinkins S.P. A wolbachia wMel transinfection in Aedes albopictus is not detrimental to host fitness and inhibits chikungunya virus. PLoS Negl. Trop. Dis. 2013;7:e2152.
Not in our library10.1371/journal.pntd.0002152
128.
Impact of Wolbachia on Infection with Chikungunya and Yellow Fever Viruses in the Mosquito Vector Aedes aegypti · 2012
No notice
129.
The Relative Importance of Innate Immune Priming in Wolbachia-Mediated Dengue Interference · 2012
No notice
130.
Pan X., Zhou G., Wu J., Bian G., Lu P., Raikhel A.S., Xi Z. Wolbachia induces reactive oxygen species (ros)-dependent activation of the toll pathway to control dengue virus in the mosquito Aedes aegypti. Proc. Natl. Acad. Sci. USA. 2012;109:E23-E31.
Not in our library10.1073/pnas.1116932108
131.
Dietary Cholesterol Modulates Pathogen Blocking by Wolbachia · 2013
No notice
132.
Zouache K., Raharimalala F.N., Raquin V., Tran-Van V., Raveloson L.H., Ravelonandro P., Mavingui P. Bacterial diversity of field-caught mosquitoes, Aedes albopictus and Aedes aegypti, from different geographic regions of Madagascar. FEMS Microbiol. Ecol. 2011;75:377-389.
Not in our library10.1111/j.1574-6941.2010.01012.x
133.
Minard G., Tran F.H., Raharimalala F.N., Hellard E., Ravelonandro P., Mavingui P., Valiente Moro C. Prevalence, genomic and metabolic profiles of acinetobacter and asaia associated with field-caught Aedes albopictus from Madagascar. FEMS Microbiol. Ecol. 2013;83:63-73.
Not in our library10.1111/j.1574-6941.2012.01455.x
134.
Reciprocal Tripartite Interactions between the Aedes aegypti Midgut Microbiota, Innate Immune System and Dengue Virus Influences Vector Competence · 2012
No notice
135.
Apte-Deshpande A.D., Paingankar M.S., Gokhale M.D., Deobagkar D.N. Serratia odorifera mediated enhancement in susceptibility of Aedes aegypti for chikungunya virus. Indian J. Med. Res. 2014;139:762-768.
Not in our library
136.
Black W.C., Bennett K.E., Gorrochotegui-Escalante N., Barillas-Mury C.V., Fernandez-Salas I., de Lourdes Munoz M., Farfan-Ale J.A., Olson K.E., Beaty B.J. Flavivirus susceptibility in Aedes aegypti. Arch. Med. Res. 2002;33:379-388.
Not in our library10.1016/s0188-4409(02)00373-9
137.
Bennett K.E., Flick D., Fleming K.H., Jochim R., Beaty B.J., Black W.C.T. Quantitative trait loci that control dengue-2 virus dissemination in the mosquito Aedes aegypti. Genetics. 2005;170:185-194.
Not in our library10.1534/genetics.104.035634
138.
Stapleford K.A., Coffey L.L., Lay S., Borderia A.V., Duong V., Isakov O., Rozen-Gagnon K., Arias-Goeta C., Blanc H., Beaucourt S., et al. Emergence and transmission of arbovirus evolutionary intermediates with epidemic potential. Cell. Host Microbe. 2014;15:706-716.
Not in our library10.1016/j.chom.2014.05.008
139.
Myers R.M., Carey D.E. Concurrent isolation from patient of two arboviruses, chikungunya and dengue type 2. Science. 1967;157:1307-1308.
Not in our library10.1126/science.157.3794.1307
140.
Hapuarachchi H.A., Bandara K.B., Hapugoda M.D., Williams S., Abeyewickreme W. Laboratory confirmation of dengue and chikungunya co-infection. Ceylon Med. J. 2008;53:104-105.
Not in our library10.4038/cmj.v53i3.252
141.
Ezzedine K., Cazanave C., Pistone T., Receveur M.C., Neau D., Ragnaud J.M., Malvy D. Dual infection by chikungunya virus and other imported infectious agent in a traveller returning from India. Travel Med. Infect. Dis. 2008;6:152-154.
Not in our library10.1016/j.tmaid.2008.02.002
142.
Co-infections with Chikungunya Virus and Dengue Virus in Delhi, India · 2009
No notice
143.
Schilling S., Emmerich P., Gunther S., Schmidt-Chanasit J. Dengue and chikungunya virus co-infection in a German traveller. J. Clin. Virol. 2009;45:163-164.
Not in our library10.1016/j.jcv.2009.04.001
144.
Nayar S.K., Noridah O., Paranthaman V., Ranjit K., Norizah I., Chem Y.K., Mustafa B., Chua K.B. Co-infection of dengue virus and chikungunya virus in two patients with acute febrile illness. Med. J. Malays. 2007;62:335-336.
Not in our library
145.
Concurrent Chikungunya and Dengue Virus Infections during Simultaneous Outbreaks, Gabon, 2007 · 2009
No notice
146.
Chang S.F., Su C.L., Shu P.Y., Yang C.F., Liao T.L., Cheng C.H., Hu H.C., Huang J.H. Concurrent isolation of chikungunya virus and dengue virus from a patient with coinfection resulting from a trip to Singapore. J. Clin. Microbiol. 2010;48:4586-4589.
Not in our library10.1128/jcm.01228-10
147.
Parreira R., Centeno-Lima S., Lopes A., Portugal-Calisto D., Constantino A., Nina J. Dengue virus serotype 4 and chikungunya virus coinfection in a traveller returning from Luanda, Angola, January 2014. Euro Surveill. 2014;19:10.
Not in our library10.2807/1560-7917.es2014.19.10.20730
148.
Caron M., Paupy C., Grard G., Becquart P., Mombo I., Nso B.B., Kassa Kassa F., Nkoghe D., Leroy E.M. Recent introduction and rapid dissemination of chikungunya virus and dengue virus serotype 2 associated with human and mosquito coinfections in Gabon, Central Africa. Clin. Infect. Dis. 2012;55:e45-e53.
Not in our library10.1093/cid/cis530
149.
Vazeille M., Mousson L., Martin E., Failloux A.B. Orally co-infected Aedes albopictus from La Reunion Island, Indian Ocean, can deliver both dengue and chikungunya infectious viral particles in their saliva. PLoS Negl. Trop. Dis. 2010;4:e706.
Not in our library10.1371/journal.pntd.0000706
150.
Courtois G., Osterrieth P., Blanes Ridaura G. Isolation of the yellow fever virus in the Belgian Congo. Ann. Soc. Belge Med. Tropicale. 1960;40:29-60.
Not in our library
151.
Osterrieth P., Rathe E., Deleplanque-Liegeois P. Simultaneous isolation of the yellow fever and chikungunya viruses at Bili (Haut-Uele, Belgian Congo). Ann. Soc. Belge Med. Tropicale. 1961;41:207-212.
Not in our library
152.
Filipe A.F., Pinto M.R. Arbovirus studies in Luanda, Angola. 2. Virological and serological studies during an outbreak of dengue-like disease caused by the chikungunya virus. Bull. World Health Organ. 1973;49:37-40.
Not in our library
153.
Gould L.H., Osman M.S., Farnon E.C., Griffith K.S., Godsey M.S., Karch S., Mulenda B., El Kholy A., Grandesso F., de Radigues X., et al. An outbreak of yellow fever with concurrent chikungunya virus transmission in South Kordofan, Sudan, 2005. Trans. R. Soc. Trop. Med. Hygiene. 2008;102:1247-1254.
Not in our library10.1016/j.trstmh.2008.04.014
154.
Zika Virus in Gabon (Central Africa) – 2007: A New Threat from Aedes albopictus? · 2014
No notice
155.
Armstrong P.M., Rico-Hesse R. Differential susceptibility of Aedes aegypti to infection by the American and Southeast Asian genotypes of dengue type 2 virus. Vector Borne Zoonotic Dis. 2001;1:159-168.
Not in our library10.1089/153036601316977769
156.
Cologna R., Armstrong P.M., Rico-Hesse R. Selection for virulent dengue viruses occurs in humans and mosquitoes. J. Virol. 2005;79:853-859.
Not in our library10.1128/jvi.79.2.853-859.2005
157.
Brault A.C., Powers A.M., Ortiz D., Estrada-Franco J.G., Navarro-Lopez R., Weaver S.C. Venezuelan equine encephalitis emergence: Enhanced vector infection from a single amino acid substitution in the envelope glycoprotein. Proc. Natl. Acad. Sci. USA. 2004;101:11344-11349.
Not in our library10.1073/pnas.0402905101
158.
Deardorff E.R., Weaver S.C. Vector competence of Culex (Melanoconion) taeniopus for equine-virulent subtype ie strains of venezuelan equine encephalitis virus. Am. J. Trop. Med. Hygiene. 2010;82:1047-1052.
Not in our library10.4269/ajtmh.2010.09-0556
159.
Sequential Adaptive Mutations Enhance Efficient Vector Switching by Chikungunya Virus and Its Epidemic Emergence · 2011
No notice
160.
Holmes E.C. The Evolution and Emergence of RNA Viruses. 2009.
Not in our library
161.
Rozen-Gagnon K., Stapleford K.A., Mongelli V., Blanc H., Failloux A.B., Saleh M.C., Vignuzzi M. Alphavirus mutator variants present host-specific defects and attenuation in mammalian and insect models. PLoS Pathog. 2014;10:e1003877.
Not in our library10.1371/journal.ppat.1003877
162.
Coffey L.L., Beeharry Y., Borderia A.V., Blanc H., Vignuzzi M. Arbovirus high fidelity variant loses fitness in mosquitoes and mice. Proc. Natl. Acad. Sci. USA. 2011;108:16038-16043.
Not in our library10.1073/pnas.1111650108
163.
Davis C.T., Ebel G.D., Lanciotti R.S., Brault A.C., Guzman H., Siirin M., Lambert A., Parsons R.E., Beasley D.W., Novak R.J., et al. Phylogenetic analysis of North American West Nile virus isolates, 2001–2004: Evidence for the emergence of a dominant genotype. Virology. 2005;342:252-265.
Not in our library10.1016/j.virol.2005.07.022
164.
Ebel G.D., Carricaburu J., Young D., Bernard K.A., Kramer L.D. Genetic and phenotypic variation of West Nile virus in New York, 2000–2003. Am. J. Trop. Med. Hygiene. 2004;71:493-500.
Not in our library
165.
Parameswaran P., Charlebois P., Tellez Y., Nunez A., Ryan E.M., Malboeuf C.M., Levin J.Z., Lennon N.J., Balmaseda A., Harris E., et al. Genome-wide patterns of intrahuman dengue virus diversity reveal associations with viral phylogenetic clade and interhost diversity. J. Virol. 2012;86:8546-8558.
Not in our library10.1128/jvi.00736-12
166.
Turner P.E., Morales N.M., Alto B.W., Remold S.K. Role of evolved host breadth in the initial emergence of an RNA virus. Evolution. 2010;64:3273-3286.
Not in our library10.1111/j.1558-5646.2010.01051.x
167.
Coffey L.L., Forrester N., Tsetsarkin K., Vasilakis N., Weaver S.C. Factors shaping the adaptive landscape for arboviruses: Implications for the emergence of disease. Future Microbiol. 2013;8:155-176.
Not in our library10.2217/fmb.12.139
168.
Coffey L.L., Vignuzzi M. Host alternation of chikungunya virus increases fitness while restricting population diversity and adaptability to novel selective pressures. J. Virol. 2011;85:1025-1035.
Not in our library10.1128/jvi.01918-10
169.
Muller H.J. The relation of recombination to mutational advance. Mutat. Res. 1964;1:2-9.
Not in our library10.1016/0027-5107(64)90047-8
170.
Weaver S.C., Brault A.C., Kang W., Holland J.J. Genetic and fitness changes accompanying adaptation of an arbovirus to vertebrate and invertebrate cells. J. Virol. 1999;73:4316-4326.
Not in our library10.1128/jvi.73.5.4316-4326.1999
171.
Duarte E.A., Clarke D.K., Moya A., Elena S.F., Domingo E., Holland J. Many-trillionfold amplification of single RNA virus particles fails to overcome the Mullerʼs ratchet effect. J. Virol. 1993;67:3620-3623.
Not in our library10.1128/jvi.67.6.3620-3623.1993
172.
Smith D.R., Adams A.P., Kenney J.L., Wang E., Weaver S.C. Venezuelan equine encephalitis virus in the mosquito vector Aedes taeniorhynchus: Infection initiated by a small number of susceptible epithelial cells and a population bottleneck. Virology. 2008;372:176-186.
Not in our library10.1016/j.virol.2007.10.011
173.
Kenney J.L., Adams A.P., Gorchakov R., Leal G., Weaver S.C. Genetic and anatomic determinants of enzootic venezuelan equine encephalitis virus infection of Culex (Melanoconion) taeniopus. PLoS Negl. Trop. Dis. 2012;6:e1606.
Not in our library10.1371/journal.pntd.0001606
174.
Forrester N.L., Guerbois M., Seymour R.L., Spratt H., Weaver S.C. Vector-borne transmission imposes a severe bottleneck on an RNA virus population. PLoS Pathog. 2012;8:e1002897.
Not in our library10.1371/journal.ppat.1002897
175.
Brackney D.E., Pesko K.N., Brown I.K., Deardorff E.R., Kawatachi J., Ebel G.D. West Nile virus genetic diversity is maintained during transmission by Culex pipiens quinquefasciatus mosquitoes. PLoS One. 2011;6:e24466.
Not in our library10.1371/journal.pone.0024466
176.
Coffey L.L., Vasilakis N., Brault A.C., Powers A.M., Tripet F., Weaver S.C. Arbovirus evolution in vivo is constrained by host alternation. Proc. Natl. Acad. Sci. USA. 2008;105:6970-6975.
Not in our library10.1073/pnas.0712130105
177.
Smith D.R., Aguilar P.V., Coffey L.L., Gromowski G.D., Wang E., Weaver S.C. Venezuelan equine encephalitis virus transmission and effect on pathogenesis. Emerg. Infect. Dis. 2006;12:1190-1196.
Not in our library10.3201/eid1708.050841
178.
Ruiz-Moreno D., Vargas I.S., Olson K.E., Harrington L.C. Modeling dynamic introduction of chikungunya virus in the United States. PLoS Negl. Trop. Dis. 2012;6:e1918.
Not in our library10.1371/journal.pntd.0001918
179.
Eisen L., Monaghan A.J., Lozano-Fuentes S., Steinhoff D.F., Hayden M.H., Bieringer P.E. The impact of temperature on the bionomics of Aedes (stegomyia) aegypti, with special reference to the cool geographic range margins. J. Med. Entomol. 2014;51:496-516.
Not in our library10.1603/me13214
180.
Zouache K., Fontaine A., Vega-Rua A., Mousson L., Thiberge J.M., Lourenco-De-Oliveira R., Caro V., Lambrechts L., Failloux A.B. Three-way interactions between mosquito population, viral strain and temperature underlying chikungunya virus transmission potential. Proc. Biol. Sci. 2014;281:1-8.
Not in our library10.1098/rspb.2014.1078
181.
Tsetsarkin K.A., Chen R., Leal G., Forrester N., Higgs S., Huang J., Weaver S.C. Chikungunya virus emergence is constrained in Asia by lineage-specific adaptive landscapes. Proc. Natl. Acad. Sci. USA. 2011;108:7872-7877.
Not in our library10.1073/pnas.1018344108
182.
Chikungunya Virus in US Travelers Returning from India, 2006 · 2007
No notice
183.
Wilke A.B., Marrelli M.T. Genetic control of mosquitoes: Population suppression strategies. Rev. Inst. Med. Trop. Sao Paulo. 2012;54:287-292.
Not in our library10.1590/s0036-46652012000500009
184.
Weaver S.C., Osorio J.E., Livengood J.A., Chen R., Stinchcomb D.T. Chikungunya virus and prospects for a vaccine. Expert Rev. Vaccines. 2012;11:1087-1101.
Not in our library10.1586/erv.12.84
185.
Paupy C., Ollomo B., Kamgang B., Moutailler S., Rousset D., Demanou M., Herve J.P., Leroy E., Simard F. Comparative role of Aedes albopictus and Aedes aegypti in the emergence of dengue and chikungunya in Central Africa. Vector Borne Zoonotic Dis. 2010;10:259-266.
Not in our library10.1089/vbz.2009.0005
186.
Turell M.J., Beaman J.R., Tammariello R.F. Susceptibility of selected strains of Aedes aegypti and Aedes albopictus (diptera: Culicidae) to chikungunya virus. J. Med. Entomol. 1992;29:49-53.
Not in our library10.1093/jmedent/29.1.49
187.
Shah K.V., Gilotra S.K., Gibbs C.J., Rozeboom L.E. Laboratory studies of transmission of chikungunya virus by mosquitoes: A preliminary report. Indian J. Med. Res. 1964;52:703-709.
Not in our library
188.
Girod R., Gaborit P., Marrama L., Etienne M., Ramdini C., Rakotoarivony I., Dollin C., Carinci R., Issaly J., Dusfour I., et al. High susceptibility to chikungunya virus of Aedes aegypti from the French West Indies and French Guiana. Trop. Med. Int. Health. 2011;16:134-139.
Not in our library10.1111/j.1365-3156.2010.02613.x
189.
Vanlandingham D.L., Hong C., Klingler K., Tsetsarkin K., McElroy K.L., Powers A.M., Lehane M.J., Higgs S. Differential infectivities of oʼnyong-nyong and chikungunya virus isolates in Anopheles gambiae and Aedes aegypti mosquitoes. Am. J. Trop. Med. Hygiene. 2005;72:616-621.
Not in our library
190.
Mangiafico J.A. Chikungunya virus infection and transmission in five species of mosquito. Am. J. Trop. Med. Hygiene. 1971;20:642-645.
Not in our library10.4269/ajtmh.1971.20.642
191.
Vazeille M., Yebakima A., Lourenco-de-Oliveira R., Andriamahefazafy B., Correira A., Rodrigues J.M., Veiga A., Moreira A., Leparc-Goffart I., Grandadam M., et al. Oral receptivity of Aedes aegypti from Cape Verde for yellow fever, dengue, and chikungunya viruses. Vector Borne Zoonotic Dis. 2013;13:37-40.
Not in our library10.1089/vbz.2012.0982
192.
Nicholson J., Ritchie S.A., van den Hurk A.F. Aedes albopictus (diptera: Culicidae) as a potential vector of endemic and exotic arboviruses in Australia. J. Med. Entomol. 2014;51:661-669.
Not in our library10.1603/me13204
193.
Tesh R.B., Gubler D.J., Rosen L. Variation among geographic strains of Aedes albopictus in susceptibility to infection with chikungunya virus. Am. J. Trop. Med. Hygiene. 1976;25:326-335.
Not in our library10.4269/ajtmh.1976.25.326
194.
Haddad N., Mousson L., Vazeille M., Chamat S., Tayeh J., Osta M.A., Failloux A.B. Aedes albopictus in Lebanon, a potential risk of arboviruses outbreak. BMC Infect. Dis. 2012;12:300.
Not in our library10.1186/1471-2334-12-300
195.
Talbalaghi A., Moutailler S., Vazeille M., Failloux A.B. Are Aedes albopictus or other mosquito species from Northern Italy competent to sustain new arboviral outbreaks? Med. Vet. Entomol. 2010;24:83-87.
Not in our library10.1111/j.1365-2915.2009.00853.x
196.
Vazeille M., Jeannin C., Martin E., Schaffner F., Failloux A.B. Chikungunya: A risk for Mediterranean countries? Acta Trop. 2008;105:200-202.
Not in our library10.1016/j.actatropica.2007.09.009
197.
Moutailler S., Barre H., Vazeille M., Failloux A.B. Recently introduced Aedes albopictus in Corsica is competent to chikungunya virus and in a lesser extent to dengue virus. Trop. Med. Int. Health. 2009;14:1105-1109.
Not in our library10.1111/j.1365-3156.2009.02320.x
198.
Kramer L.D., Chin P., Cane R.P., Kauffman E.B., Mackereth G. Vector competence of New Zealand mosquitoes for selected arboviruses. Am. J. Trop. Med. Hygiene. 2011;85:182-189.
Not in our library10.4269/ajtmh.2011.11-0078
199.
Jupp P.G., McIntosh B.M., Dos Santos I., DeMoor P. Laboratory vector studies on six mosquito and one tick species with chikungunya virus. Trans. R. Soc. Trop. Med. Hygiene. 1981;75:15-19.
Not in our library10.1016/0035-9203(81)90005-5
200.
Aedes hensilli as a Potential Vector of Chikungunya and Zika Viruses · 2014
No notice
201.
Diagne C.T., Faye O., Guerbois M., Knight R., Diallo D., Faye O., Ba Y., Dia I., Faye O., Weaver S.C., et al. Vector competence of Aedes aegypti and Aedes vittatus (diptera: Culicidae) from Senegal and Cape Verde archipelago for West African lineages of chikungunya virus. Am. J. Trop. Med. Hygiene. 2014;91:635-641.
Not in our library10.4269/ajtmh.13-0627

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Mosquito-borne arboviruses of African origin: review of key viruses and vectors · 2018
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Chikungunya virus: clinical aspects and treatment - A Review · 2017
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Impacto general de las fiebres del Zika y Chikungunya en Colombia y América del sur: Análisis general de los virus y su importancia · 2016
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Differential Susceptibilities of Aedes aegypti and Aedes albopictus from the Americas to Zika Virus · 2016
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The wMel Strain of Wolbachia Reduces Transmission of Chikungunya Virus in Aedes aegypti · 2016
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