Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies
Hee-Jin Jeong, Jinhua Dong, Hiroshi Ueda
Sensors · 2018
doi:10.3390/s19010052 (opens in a new tab)
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Abstract
We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.
Abstract licence: CC BY
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