Kupffer Cells Survive Plasmodium berghei Sporozoite Exposure and Respond with a Rapid Cytokine Release

The liver stage of the <i>Plasmodium</i> life cycle features sporozoite traversal of the liver sinusoidal barrier through Kupffer cells (KCs) followed by invasion of hepatocytes. Little is known about the interaction of <i>Plasmodium</i> sporozoites with KCs, the liver-resident macrophages. Previous reports suggest KCs do not mount a pro-inflammatory response and undergo cell death following this interaction. Our work explores this interaction using primary rat KCs (PRKCs) and <i>Plasmodium berghei</i> sporozoites. We analyzed PRKC culture supernatants for markers of an immunological response through cytokine arrays. Additionally, cell wounding and death were assessed by monitoring lactate dehydrogenase (LDH) levels in these supernatants and by live/dead cell imaging. We found that PRKCs mount an immunological response to <i>P. berghei</i> sporozoites by releasing a diverse set of both pro- and anti-inflammatory cytokines, including IFN&#947;, IL-12p70, Mip-3&#945;, IL-2, RANTES, IL-1&#945;, IL-4, IL-5, IL-13, EPO, VEGF, IL-7, and IL-17&#945;. We also observed no difference in LDH level or live/dead staining upon sporozoite exposure, suggesting that the KCs are not deeply wounded or dying. Overall, our data suggest that sporozoites may be actively modulating the KC&#8217;s reaction to their presence and altering the way the innate immune system is triggered by KCs.

Abstract licence: CC BY

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