Simultaneous Determination of α-Glucosidase Inhibitory Triterpenoids in Psidium guajava Using HPLC–DAD–ELSD and Pressurized Liquid Extraction

<i>Psidium guajava</i>, a popular food and medicine dual purposes plant cultivated in tropical and subtropical regions, has been widely used as food crop and folk medicine, such as anti-diabetes agent, around the world. Triterpenoids have been considered as the major active ingredients of <i>P. guajava</i>. In the present study, a high-performance liquid chromatography coupled with diode array and evaporative light scattering detectors (HPLC&#8722;DAD&#8722;ELSD) method was developed for simultaneous determination of nine triterpenoids in <i>P. guajava</i>. Pressurized liquid extraction (PLE) was performed for sample preparation, and the analysis was achieved on a Cosmosil 5C18-MS-II (Nacalai Tesque, Kyoto, Japan) column eluted with gradient 0.1% aqueous formic acid-methanol system. The drift tube temperature of ELSD was set at 40 &#176;C, and nitrogen flow-rate was at 1.6 L/min. All calibration curves for the analytes showed good linear regression (<i>R²</i> &gt; 0.9992) within test ranges. The established method was validated for intra-day and inter-day precisions (RSDs &lt; 5%) and accuracy (recovery 94.23&#8722;106.87%). The validated method was successfully applied to determinate nine triterpenoids in 15 samples from the leave or fruit of P. guajava. In addition, the &#945;-glucosidase inhibition assay showed good &#945;-glucosidase inhibition activity in almost all the determined triterpenoids. The present study suggested that triterpenoids should be the quality control markers for <i>P. guajava</i> and HPLC&#8722;DAD&#8722;ELSD was an effective tool for the quality control of <i>P. guajava</i>.

Abstract licence: CC BY

A person counts once a day per paper. We keep no cookie and no address: a reader is a hash that changes every day. Any month under five reads shows no number.