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Chitosan Effects on Plant Systems

Massimo Malerba, Raffaella Cerana

International Journal of Molecular Sciences · 2016 · Review · CC BY

doi:10.3390/ijms17070996

Abstract

Chitosan (CHT) is a natural, safe, and cheap product of chitin deacetylation, widely used by several industries because of its interesting features. The availability of industrial quantities of CHT in the late 1980s enabled it to be tested in agriculture. CHT has been proven to stimulate plant growth, to protect the safety of edible products, and to induce abiotic and biotic stress tolerance in various horticultural commodities. The stimulating effect of different enzyme activities to detoxify reactive oxygen species suggests the involvement of hydrogen peroxide and nitric oxide in CHT signaling. CHT could also interact with chromatin and directly affect gene expression. Recent innovative uses of CHT include synthesis of CHT nanoparticles as a valuable delivery system for fertilizers, herbicides, pesticides, and micronutrients for crop growth promotion by a balanced and sustained nutrition. In addition, CHT nanoparticles can safely deliver genetic material for plant transformation. This review presents an overview on the status of the use of CHT in plant systems. Attention was given to the research that suggested the use of CHT for sustainable crop productivity.

Full text

From PubMed Central, U.S. National Library of Medicine, as current on Oct 21, 2026. Paragraph numbers are ours, so an answer can point at one.

1. Introduction

Chitosan is a natural, safe, and cheap biopolymer produced from chitin, the major constituent of arthropods exoskeleton and fungi cell walls and the second renewable carbon source after lignocellulosic biomass. For industrial production, solid chitin is soaked in 40%–50% (w/v) NaOH. This process removes more than 80% of the acetyl residues and converts N-acetyl-d-glucosamine into β-1,4-d-glucosamine. The CHT preparations are heterogeneous as for deacetylation degree, molecular mass, polymerization degree, viscosity, acid dissociation constant (pKa value), and the term “chitosan” does not describe a unique compound, but a group of commercially available copolymers. This heterogeneity can greatly affect the physical properties of CHT, thus governing its biological applications.

In addition to its low-cost of production, CHT also possesses several favorable biological properties such as biodegradability, biocompatibility and non-allergenicity. CHT is susceptible to degradation by specific and nonspecific enzymes, and it shows low toxicity to humans. All these characteristics make CHT very useful for several industries, namely cosmetology, food, biotechnology, pharmacology, and medicine.

The availability of industrial quantities of CHT in the 1980s enabled it to be tested as an agricultural tool. The results indubitably show that CHT can control many pre- and postharvest diseases on different crops. CHT treatment makes plants more tolerant to a wide range of soil and foliar pathogens and induces root nodulation, thus proposing CHT as a useful tool for agricultural sustainability. These published experimental data have been summarized in recent comprehensive reviews. The huge value of these results motivated worldwide researchers to find more applications for CHT and its derivatives.

This paper reviews the current and ongoing research in these areas and it is organized into three sections: (1) physiological responses to CHT in plants; (2) application of CHT on crop/food plants; (3) use of CHT nanoparticles in agriculture as a delivery system.

2. Physiological Responses to CHT in Plants

CHT has been demonstrated to be a natural molecule that induces numerous biological responses in plants, dependent on its structure and concentration and on species and developmental stage of the plant. After the first report on the eliciting action of CHT in pea (Pisum sativum L.) and tomato (Solanum lycopersicum L.) plants, CHT was shown to enhance defense responses to abiotic and biotic stresses. An initial oxidative burst with hydrogen peroxide (H2O2) accumulation was observed in different plants supplied with CHT as well as in plant cell cultures. It is thought that this can lead to the induction of plant defense enzymes, and to the synthesis of secondary metabolites, such as polyphenolics, lignin, flavonoids, and phytoalexins observed in many plant species treated with CHT. These species include but are not limited to sunflower (Helianthus annuus L.), papaya (Carica papaya L.), litchi (Litchi chinensis), grape (Vitis vinifera L.), apricot (Prunus armeniaca L.), loquat (Eriobotrya japonica), soybean (Glycine max L.), tomato (Solanum lycopersicum L.), Greek oregano (Origanum vulgare ssp. hirtum), butter lettuce (Lactuca sativa L.), sweet basil (Ocimum basilicum L.), and spinach (Spinacia oleracea). Other biochemical and molecular changes observed in plants fed with CHT include: callose apposition, increases in cytosolic Ca2+, activation of MAP-kinases, plasma membrane H+-ATPase inhibition, chromatin alterations, synthesis of alkaloids, and phytoregulators (jasmonic acid, JA, and abscisic acid, ABA).

2.1. Mechanism of CHT Action

The mode of action of CHT is not yet completely unraveled. The different mechanisms for its antimicrobial effect, summarized in Table 1, include interaction with plasma membrane phospholipids and histone proteins as well as chelation of trace metal elements.

For induction of defense responses in plants, CHT may directly affect gene expression interacting with chromatin and/or it may bind to specific receptors. A CHT-binding protein belonging to the glycoprotein family of lectines has been isolated from leaves of Brassica campestris L. (ssp. chinensis (L.) Makino). The presence of CHT receptors is also suggested by the rapid activation of plasma membrane H+-ATPase in isolated vesicles from pulvini of Mimosa pudica and Cassia fasciculata. In addition, CHT can induce a receptor-like kinase gene and a MAP kinase pathway, and the lysin motif receptor-like kinase chitin elicitor receptor kinase 1 (CERK1) seems to bind chitin and CHT as suggested by experiments with A. thaliana knockout mutants. Interestingly, the lysin motif domains of CERK1 are also present in different legume receptors of Nod factors, chitin-related molecules produced by nitrogen-fixing bacteria. On the other hand, in Arabidopsis seedlings Povero et al. reported that the signaling by CHT does not use CERK1 and is perceived through a CERK1-independent pathway. Thus, the categorization of CERK1 as a CHT receptor is still uncertain and CHT remains “a Pathogen-Associated Molecular Pattern (PAMP) in search of a Pattern Recognition Receptor (PRR)”.

2.2. Signals Inside the Cell

After the recognition of the CHT molecule by a specific cellular receptor, second messenger(s) must transduce the signal to induce the physiological responses. Several papers report the involvement of molecules like reactive oxygen species (ROS), Ca2+, nitric oxide (NO), phytohormones in the CHT-mediated signaling pathway. As reported previously in this paper ROS, in particular H2O2 regulates several responses induced by CHT in many plant species. Ca2+ regulates callose synthase activity in response to CHT elicitation in both monocotyledonous and dicotyledonous species, mediates the programmed cell death induced by CHT in soybean cells and the cell death kinetic induced by CHT was delayed by treatment with a calcium channel blocker during tobacco necrosis virus infection of tobacco plants. The possible signaling role of NO has been investigated in pearl millet seedlings where the degree of protection by CHT against pathogens was decreased by treatment with the NO synthase inhibitor LNAME (N-nitro-l-arginine methyl ester hydrochloride) or with the NO scavenger c-PTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt] and in tomato cell cultures, where c-PTIO applied together with CHT decreased the level of phosphatidic acid (PA) production after pathogen attacks. In tobacco leaves, c-PTIO and LNAME partly blocked the activation of Ser/Thr protein kinases and the activities of many defense-related enzymes induced by a chitooligosaccharide while the NO donor sodium nitroprusside induced the activation of kinases and enhanced the defense systems. Recently, Malerba and Cerana obtained similar results in sycamore (Acer pseudoplatanus L.) cultured cells where c-PTIO prevented several stress responses induced by CHT. As far as phytohormones are concerned, CHT induced a fast accumulation of JA in rice and Phaseolus vulgaris. A cDNA microarray/semiquantitative RT-PCR analyses of Brassica napus gene expression changes induced by CHT showed that CHT activated the plant self-defense through JA/ethylene signaling pathway. In different plant/pathogen interactions, the degree and kinetics of callose synthesis are regulated by ABA. Interestingly, the ABA inhibitor nordihydroguaiaretic acid, applied before CHT, decreased both callose synthesis and plant resistance to tobacco necrosis virus thus indicating the involvement of ABA in the CHT action. Finally, the phytohormones indole-3-acetic acid and kinetin stimulated growth and CHT production of the fungus Mucor indicus.

Collectively, these results indicate that CHT may activate the plant responses through different signaling pathways involving different second messengers. In spite of this research, the mode of action of chitosan have not yet been elucidated clearly. This highlights the necessity for more studies in the future.

3. Application of CHT on Crop/Food Plants

The effect of CHT has been investigated in several crops, including cereal, ornamental, fruit, and medicinal plants.

3.1. Antipathogen Activities of CHT

The first study on antipathogen activity of CHT was published in 1979 by Allan and Hadwiger. This study reported the fungicidal effect of CHT on fungi of different cell wall composition. This attracted the attention of agricultural industry on CHT since pathogens can cause severe diseases and relevant losses in crop yield and quality worldwide. Many pathogens can also produce toxins and metabolites, which can greatly affect the safety of agricultural products. Many studies reported the antimicrobial properties of CHT and its derivatives and many plant pathogens resulted sensitive to CHT. Many studies deal with CHT effect on fungi. In fact, during both pre- and postharvest processes, infection of pathogenic fungi results in major losses of fruits and vegetables. At present, synthetic chemical fungicides are the primary choice to manage these pathogens. However, synthetic fungicides are potentially harmful on human health and their indiscriminate use induces the emergence of resistant strains. CHT is a promising alternative to control these diseases. In fact, it has been shown to possess a broad-spectrum fungicidal activity against several phytopathogenic fungi, effectively inhibiting their development at different life-cycle stages. For example, in pear (Pyrus pyrifolia L.) fruit CHT completely prevented germination and growth of Alternaria kikuchiana and Physalospora piricola. CHT inhibited growth of Botrytis cinerea in liquid culture and suppressed grey mold disease caused by the fungus on detached grapevine leaves and bunch rot in Chardonnay and Sauvignon blanc wine grapes. In rice (Oryza sativa) CHT showed marked antifungal activity against Rhizoctonia solani, the rice sheath blight pathogen. Cowpea (Vigna unguiculata L.) plants were protected by CHT against Fusarium oxysporum infection. CHT combined with clove oil or Bacillus subtilis endospores protected mature satsuma mandarin (Citrus unshiu Marc. cv. Miyagawawase) or Shogun mandarin oranges (Citrus reticulate Blanco cv. Shogun) fruits against Penicillium digitatum, the causal agent of citrus green mold. Dragon fruit (Hylocereus polyrhizus) plants were protected by CHT against Colletotrichum gloeosporioides. CHT enriched with lemongrass oil was very effective in in vivo and in vitro control of anthracnose caused by Colletotrichum capsici in bell pepper (Capsicum annuum L.). Finally, cell development and physiology of Ustilago maydis, the dimorphic fungus causing corn smut disease, were affected by CHT and glycol chitosan. Scots pine (Pinus sylvestris L.) seedlings sprayed with CHT were effectively protected against parasitic damping-off and Lophodermium needle cast and CHT induced cell death in spores of Fusarium eumartii, a fungal pathogen of potato and tomato. CHT also prevented the growth of several pathogenic bacteria including Xanthomonas, Pseudomonas syringae, Escherichia coli, Acidovorax citrulli, Agrobacterium tumefaciens, and Erwinia carotovora. However, CHT is generally less effective against bacteria than fungi and it often shows a higher effect on Gram-positive than Gram-negative bacteria, possibly due to the different composition of bacterial cell wall differently affecting the cellular entry of macromolecules such as CHT. Compared with researches on antifungal and antibacterial action of CHT, its antiviral effect in plants is less documented. In bean plants, CHT induced resistance to the systemic pathogen bean mild mosaic virus. In tobacco plants, CHT inhibited the appearance of localized necrotic lesions by systemic tobacco mosaic virus by 50%–90% and the multiplication and movement of tobacco necrosis virus. Recently, a protective action of CHT against other affecting plants pathogenic organisms has been also reported. Growth and larval vitality of oleander aphid Aphis nerii and cotton leafworm Spodoptera littoralis were severely affected by CHT. CHT was very effective against the root-knot nematode Meloidogyne javanica in vitro and in vivo under greenhouse conditions. The number of nymphs of the spear psylla, Cacopsylla pyricola (Foerster) (Hemiptera: Psyllidae), the key pest of cultivated pear (Pyrus communis L.), present on plants 30 days after releasing 10 adults on the tree was significantly reduced by foliar application of CHT. Interestingly, the aphid predators ladybird (Propylea japonica, Coleoptera: Coccinellidae) fed with microcapsulae of Ca-alginate and CHT showed improved respiration and predation abilities compared to those fed with the liquid artificial diet usually utilized for mass rearing of predatory insects for biological control.

3.2. Stimulant Activity of CHT on Growth of Horticultural Plant

Recent studies on CHT use in horticultural plants are reported below. Foliar application of CHT enhanced fruit weight and productivity in tomato and fruit yield, plant height, and leaf number in okra (Hibiscus esculentus L.). The application of CHT stimulated plant growth in Greek oregano and increased biomass accumulation in cell cultures of three Ocimum species. The soil addition of CHT increased height, canopy diameter, and leaf area of Capsicum annuum L.. Seed dressing and foliar spraying at different growth stages with CHT impacted wheat (Triticum aestivum L.) production in large-scale field experiments by improving the yield components, such as the spike number and grains per spike. CHT efficiently improved microspore embryogenesis and plantlet regeneration in Brassica napus L.. Somatic embryogenesis in cultures of Actinidia deliciosa was enhanced by CHT. CHT can also promote growth of flower species. CHT enhanced seed germination percentage of Dendrobium formosum orchid but was ineffective on Dendrobium bigibbum var. compactum. Exposure of gladiolus corms (Gladiolus communis L.) to CHT accelerated the emergence and augmented the number of cormlets and flowers and prolonged their vase life. Similarly, CHT application to freesia corms (Freesia Eckl. ex Klatt) led to taller plants, with more leaves, shoots, flowers, and corms. The vase life of cut rose flowers (Rosa hybrida L.) “Red France” was also prolonged by CHT. The largest number of studies on the effects of CHT deal with fruit crops. Foliar spraying with CHT enhanced coffee plant growth, and CHT spraying of strawberries (Fragaria chiloensis L.) stimulated vegetative growth and yield. Dipping grapevine cuttings in CHT solution enhanced rooting and number of internodes. Strawberry plants sprayed with CHT at different developmental stages produced fruits with increased shelf life. The yield of tomato plants increased with CHT treatment.

CHT-based compounds can be also be used to coat or dip detached fruits, and this teatment prolongs shelf life and retards deterioration of many fruits such as cucumber, carrot, apple, citrus, kiwifruit, peach, pear, strawberry, and sweet cherry. In fact, the protective barrier formed by CHT reduces water loss, inhibits gas exchange, decreases nutrient loss, and prevents microorganism growth on fruit surface. Recent results have shown that CHT prolonged storage life and controlled decay of commercially important tropical fruits such as papaya and mango fruits. CHT protected citrus (Citrus limon L.) fruit from mold disease caused by Penicillium spp. phytopathogenic fungi. Fragaria chiloensis fruits treated with CHT and methyl jasmonate maintained higher levels of fruit firmness, anthocyanin, and showed significant delays in decay incidence compared to control fruit. Shelf life of red kiwifruit (Actinidia melanandra) was extended by CHT coating. Coating with CHT and trans-cinnamaldehyde improved structural integrity and antioxidant metabolism of fresh-cut Cantaloupe melons (Cucumis melo var. Cantalupensis Naud.). CHT coating improved quality and nutraceutical traits of loquat fruit (Eriobotrya japonica Lindl.) during postharvest life. Postharvest CHT-g-salicylic acid application alleviated chilling injury and preserves cucumber (Cucumis sativus L.) fruit quality during cold storage through increased levels of salicylic acid and antioxidant enzymes.

4. Use of CHT Nanoparticles in Agriculture

The biggest goal of modern agriculture is to produce food of good quality and in sufficient quantity to meet the rise in world population limiting environmental impacts. Agricultural production is severely affected by many pests and diseases that can cause considerable losses. During the past 100 years, chemical fertilizers and pesticides were used to face these problems and increase yield. The huge utilization of these products raised productivity significantly, but it also led to reduced biological diversity and degraded natural and agricultural systems. In addition, residue accumulation led to environmental pollution and public health problems, with development of resistant pests. Therefore, alternative methods are necessary to face these problems of reducing the environmental impact of the activity without affecting agricultural productivity and with economic benefits for farmers. In this context, in recent years nanotechnology has emerged as a potential tool to greatly improve stress resistance and nutrients or chemicals uptake. Nanotechnology-derived devices are currently employed as delivery systems to combat crop pathogens, to minimize nutrient losses during fertilization, and to improve yield. The encapsulation of different chemicals in slow-release particles can be very valuable for sustainable agriculture and food safety. Recently, CHT-based materials have been used to produce nanoparticles able to efficiently supply plants with chemicals and nutrients. In fact, CHT easily absorbs to epidermis of leaves and stems prolonging the contact time and facilitating the uptake of the bioactive molecules.

4.1. Pesticide Delivery

In recent years, different studies describe the synthesis and utilization of CHT nanoparticles as a delivery system of pesticides. In addition, the encapsulation in CHT nanoparticles helps solve problems of solubility. For example, CHT microspheres were used to encapsulate synthetic brassinosteroids and diosgenin derivatives. The water-insoluble botanical insecticide rotenone was encapsulated in nanomicelles composed of an amphiphilic derivative of CHT, increasing its solubility, and a material composed by carboxymethyl CHT and ricinoleic acid was synthesized and used to improve water solubility of the biopesticide azadirachtin. These in vitro results encouraged in vivo studies. For example, microspheres composed of CHT and cashew tree gum were used to carry the essential oil of Lippia sidoides, an efficient insecticide against A. aegypti larvae and microcapsules of CHT and sodium alginate were prepared and used for efficient imidacloprid release against the coleopteron Martianus dermestoides.

More recently, nanoparticles of CHT and sodium tripolyphosphate were prepared and successfully evaluated for suppression of rice blast fungus (Pyricularia grisea) and oleoyl-chitosan nanoparticles were synthesized and used to disperse antifungal products.

4.2. Fertilizer and Micronutrient Delivery

It is well known that the availability of sufficient quantity of fertilizer and water as well as of microelements, i.e. Mn, Cu, B, Mo, Fe, Cl, and Zn, promotes optimal growth. Therefore, any improvement in plant yield requires a better utilization of growth nutrients and water resources. At present, it is estimated that the intended target plants cannot absorb about 80%–90% of phosphorus, 40%–70% of nitrogen, and 50%–70% of potassium contained in fertilizers. This resource loss is not only a waste of money but it also results in severe environmental pollution. Farmers must move toward more efficient and sustainable practices to face the increase of fertilizer cost and the desire for environmentally-friendly agriculture. In this context, CHT-based polymers may be the solution. Nanoparticles composed by an inner coating of CHT, an outer coating of crosslinked poly(acrylic acid)/diatomite—containing urea and a core of water-soluble granular nitrogen (N), phosphorus (P), and potassium (K) (NPK) fertilizer showed slow-controlled release of the nutrients without any detrimental impact on the soil. A similar investigation was also performed by Corradini et al., while Hussain et al. encapsulated urea in CHT microspheres obtaining a controlled release of the nutrient. Recently, a potassium-containing, controlled-release biodegradable material based on CHT and montmorillonite clay layered silicate was prepared, and its structural, thermal, and morphological characterization was performed and a new, super water-absorbing, material based on CHT, EDTA, and urea was synthesized. This new material is very promising for its capacity to slow the release of urea and water as well as of metal ions that can be attached through the EDTA component.

It should be noted that the Green Revolution coupled with farming practices such as liming to increase crop productivity has progressively depleted soil level and availability of essential micronutrients such as boron, iron, copper, nickel, zinc, and molybdenum. Up to now, micronutrients are commonly applied to leaves and nanoformulations of micronutrients may be sprayed on the leaves to promote absorption.

Alternatively, use of nanoparticles with micronutrients as a soil addition has been tested and for their low toxicity those made from organic compounds like lipids, chitin, and CHT are more promising than the metal-made ones.

For an example, the delivery of microelements such as Zn2+ and Cu2+ was enhanced by cross-linked CHT/suberoyl chloride particles. The same approach was used for the controlled delivery of other molecules important for plant growth. For example, CHT particles coupled with the phytohormone 1-naphthylacetic acid were synthesized and appeared able to slowly release the hormone. These results are summarized in Table 2.

Over all, although preparation of nanoparticles is quite expensive, the lower use of nutrients to enhance plant productivity as well as the absence of undesirable environmental impact are compensatory benefits for the initial high cost.

4.3. Herbicide Delivery

In modern agriculture, the utilization of herbicides greatly increased to reduce the loss in food plants productivity and this excessive utilization caused severe environmental and human health problems, herbicides being the phytochemicals most widely contaminating hydrological systems. To manage these problems, the use of natural polysaccharides like CHT was investigated. For example, several authors synthesized and tested modified CHT nanoparticles to carry paraquat, the most widely used herbicide. Silva et al. demonstrated that alginate/CHT nanoparticles alter the release of the herbicide and its interaction with the soil. In addition, CHT/tripolyphosphate nanoparticles decreased paraquat toxicity, and improved herbicidal activity against Eichhornia crassipes was observed when paraquat was encapsulated in silver/CHT nanoparticles. Besides, an approach based on CHT nanoparticles may be used to monitor and remove excess of herbicide or heavy metals from soil and water. A nanocomposite material of CHT and montmorillonite was used to adsorb and remove the herbicide clopyralid present in water and soil. Wen et al. demonstrated that CHT enhances the bioavailability of the chiral herbicide dichlorprop to the green alga Chlorella pyrenoidosa, and a plant esterase-CHT/gold nanoparticles−graphene nanosheet composite-based material was successfully tested for ultrasensitive detection of organophosphate pesticides in different samples. CHT microcapsules from pollens of Acer negundo, Cupressus sempervirens, and Populus nigra were used to remove trace elements. Similarly, spores of the phytopathogenic microfungi Ustilago maydis and U. digitariae were immobilized in cross-linked CHT matrix and used to remove trace elements from water.

4.4. Genetic Transformation

For efficient uptake of biological molecules such as DNA and plasmids into the plant cell, the biggest challenge is the cell wall. Traditional methods of delivery are expensive, relatively inefficient, and unsuccessful for genetic transformation of important crops such as monocotyledons. Thus, the development of new methods based on nanotechnology is an important goal to obtain successful monocot and dicot transformants. In particular, the potential of use of CHT for genetic transformation is suggested by its capability to form, through electrostatic interactions, a complex where DNA is protected from nuclease degradation. Interesting results were obtained by Wang et al., who prepared QD-labeled CHT-DNA complexes to monitor nanoparticle-mediated genetic transformation of cultured cells of Jatropha curcas. This method gave rise to stable transformants with higher efficiency than other traditional methods of gene delivery.

Recent results showed that CHT-based nanoparticles with highly positive surface coatings can passively penetrate across the chloroplast membrane. Once in the chloroplast these nanoparticles exhibit both confined diffusion and convection, before reaching an irreversibly trapped state. These results suggest that CHT nanoparticles can be used as possible molecular transporters into plastids like the chloroplast.

5. Conclusions

CHT induces numerous biological responses in plants such as stress resistance and increased productivity, which depend on CHT chemical composition and the timing and rate of application. Despite the huge amount of work, the mode of action of CHT is not yet completely unraveled and further transcriptomic and proteomic studies of defense genes and proteins are needed to fully understand the complex CHT-mediated responses, thus enabling a better use in plant disease control. Chitosan, as a unique abundant biopolymer, has a promising future in development of sustainable agricultural practices as well as in food production and preservation.

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    Not in our library10.1094/phyto-98-4-0372

  29. 29.

    Iriti M., Faoro F. Chitosan as a MAMP searching for a PRR. Plant Signal. Behav. 2009;4:66-68.

    Not in our library10.4161/psb.4.1.7408

  30. 30.

    Orlita A., Sidwa-Gorycka M., Paszkiewicz M., Malinski E., Kumirska J., Siedlecka E.M. Application of chitin and chitosan as elicitors of coumarins and fluoroquinolone alkaloids in Ruta graveolens L. (common rue). Biotech. Appl. Biochem. 2008;51:91-96.

    Not in our library10.1042/ba20070200

  31. 31.

    Doares S.H., Syrovets T., Weiler E.W., Ryan C.A. Oligogalacturonides and chitosan activate plant defence genes through the octadecanoic pathway. Proc. Natl. Acad. Sci. USA. 1995;92:4095-4098.

    Not in our library10.1073/pnas.92.10.4095

  32. 32.

    Iriti M., Faoro F. Abscisic acid mediates the chitosan-induced resistance to tobacco necrosis virus (TNV). Plant Physiol. Biochem. 2008;46:1106-1111.

    Not in our library10.1016/j.plaphy.2008.08.002

  33. 33.

    Sudarshan N.R., Hoover D.G., Knorr D. Antibacterial action of chitosan. Food Biotechnol. 1992;6:257-272.

    Not in our library10.1080/08905439209549838

  34. 34.

    Liang C., Yuan F., Liu F., Wang Y., Gao Y. Structure and antimicrobial mechanism of ε-polylysine-chitosan conjugates through Maillard reaction. Int. J. Biol. Macromol.,. 2014;70:427-434.

    Not in our library10.1016/j.ijbiomac.2014.07.012

  35. 35.

    Xing K., Chen X.G., Liu C.S., Cha D.S., Park H.J. Oleoyl-chitosan nanoparticles inhibits Escherichia coli and Staphylococcus aureus by damaging the cell membrane and putative binding to extracellular or intracellular targets. Int. J. Food Microbiol. 2009;132:127-133.

    Not in our library10.1016/j.ijfoodmicro.2009.04.013

  36. 36.

    García-Rincóna J., Vega-Pérez J., Guerra-Sánchez M.G., Hernández-Lauzardo A.N., Peña-Díaz A., Velázquez-Del Valle M.G. Effect of chitosan on growth and plasma membrane properties of Rhizopus stolonifer (Ehrenb.:Fr.) Vuill. Pestic. Biochem. Phys. 2010;97:275-278.

    Not in our library10.1016/j.pestbp.2010.03.008

  37. 37.

    Geisberger G., Gyenge E.B., Hinger D., Käch A., Maake C., Patzke G.R. Chitosan-thioglycolic acid as a versatile antimicrobial agent. Biomacromolecules. 2013;14:1010-1017.

    Not in our library10.1021/bm3018593

  38. 38.

    Reddy M.V.B., Arul J., Ait-Barka E., Angers P., Richard C., Castaigne F. Effect of chitosan on growth and toxin production by Alternaria alternata f. sp. lycopersici. Biocontrol. Sci. Technol. 1998;8:33-43.

    Not in our library10.1080/09583159830414

  39. 39.

    Liu X.F., Guan Y.L., Yang D.Z., Li Z., Yao K.D. Antibacterial action of chitosan and carboxymethylated chitosan. J. Appl. Polym. Sci. 2001;79:1324-1335.

    Not in our library

  40. 40.

    Vishu K.A.B., Varadaraj M.C., Gowda L.R., Tharanathan R.N. Characterization of chito-oligosaccharides prepared by chitosanolysis with the aid of papain and Pronase, and their bactericidal action against Bacillus cereus and Escherichia coli. Biochem. J. 2005;391:167-175.

    Not in our library10.1042/bj20050093

  41. 41.

    Rabea E.I., Badawy M.E.T., Stevens C.V., Smagghe G., Steurbaut W. Chitosan as antimicrobial agent: Applications and mode of action. Biomacromolecules. 2003;4:1457-1465.

    Not in our library10.1021/bm034130m

  42. 42.

    Hadwiger L.A. Anatomy of a nonhost disease resistance response of pea to Fusarium solani: PR gene elicitation via DNase, chitosan and chromatin alterations. Front. Plant Sci. 2015;12.

    Not in our library10.3389/fpls.2015.00373

  43. 43.

    Chen H.P., Xu L.L. Isolation and characterization of a novel chitosan-binding protein from non-heading Chinese cabbage leaves. J. Integr. Plant Biol. 2005;47:452-456.

    Not in our library10.1111/j.1744-7909.2005.00022.x

  44. 44.

    Petutschnig E.K., Jones A.M.E., Serazetdinova L., Lipka U., Lipka V. The Lysin Motif Receptor-like Kinase (LysM-RLK) CERK1 is a major chitin-binding protein in Arabidopsis thaliana and subject to chitin-induced phosphorylation. J. Biol. Chem. 2010;285:28902-28911.

    Not in our library10.1074/jbc.m110.116657

  45. 45.

    Kaku H., Nishizawa Y., Ishii-Minami N., Akimoto-Tomiyama C., Dohmae N., Takio K., Minami E., Shibuya N. Plant cells recognize chitin fragments for defence signalling through a plasma membrane receptor. Proc. Natl. Acad. Sci. USA. 2006;103:11086-11091.

    Not in our library10.1073/pnas.0508882103

  46. 46.

    Povero G., Loreti E., Pucciariello C., Santaniello A., Di Tommaso D., Di Tommaso G., Kapetis D., Zolezzi F., Piaggesi A., Perata P. Transcript profiling of chitosan-treated Arabidopsis seedings. J. Plant Res. 2011;124:619-629.

    Not in our library10.1007/s10265-010-0399-1

  47. 47.

    Faoro F., Maffi D., Cantù D., Iriti M. Chemical-induced resistance against powdery mildew in barley: The effects of chitosan and benzothiadiazole. BioControl. 2008;53:387-301.

    Not in our library10.1007/s10526-007-9091-3

  48. 48.

    Iriti M., Sironi M., Gomarasca S., Casazza A.P., Soave C., Faoro F. Cell death-mediated antiviral effect of chitosan in tobacco. Plant Physiol. Biochem. 2006;4:893-900.

    Not in our library10.1016/j.plaphy.2006.10.009

  49. 49.

    Hadwiger L.A. Multiple effects of chitosan on plant systems: Solid science or hype. Plant Sci. 2013;208:42-49.

    Not in our library10.1016/j.plantsci.2013.03.007

  50. 50.

    Raho N., Ramirez L., Lanteri M.L., Gonorazky G., Lamattina L., ten Have A., Laxalt A.M. Phosphatidic acid production in chitosan-elicited tomato cells, via both phospholipase D and phospholipase C/diacylglycerol kinase, requires nitric oxide. J. Plant Physiol. 2011;168:534-539.

    Not in our library10.1016/j.jplph.2010.09.004

  51. 51.

    Zhang H., Zhao X., Yang J., Yin H., Wang W., Lu H., Du Y. Nitric oxide production and its functional link with OIPK in tobacco defense response elicited by chitooligosaccharide. Plant Cell Rep. 2011;30:1153-1162.

    Not in our library10.1007/s00299-011-1024-z

  52. 52.

    Malerba M., Cerana R. Reactive oxygen and nitrogen species in defense/stress responses activated by chitosan in sycamore cultured cells. Int. J. Mol. Sci. 2015;16:3019-3034.

    Not in our library10.3390/ijms16023019

  53. 53.

    Rakwal R., Tamogami S., Agrawal G.K., Iwahashi H. Octadecanoid signaling component “burst” in rice (Oryza sativa L.) seedling leaves upon wounding by cut and treatment with fungal elicitor chitosan. Biochem. Biophys. Res. Commun. 2002;295:1041-1045.

    Not in our library10.1016/s0006-291x(02)00779-9

  54. 54.

    Yin H., Li S., Zhao X., Du Y., Ma X. cDNA microarray analysis of gene expression in Brassica napus treated with oligochitosan elicitor. Plant Physiol. Biochem. 2006;44:910-916.

    Not in our library10.1016/j.plaphy.2006.10.002

  55. 55.

    Flors V., Ton J., Jakab G., Mauch-Mani B. Abscisic acid and callose: Team players in defence against pathogens? J. Phytopathol. 2005;153:377-383.

    Not in our library10.1111/j.1439-0434.2005.00987.x

  56. 56.

    Safaei Z., Karimi K., Golkar P., Zamani A. Effects of plant growth hormones on Mucor indicus growth and chitosan and ethanol production. Int. J. Mol. Sci. 2015;22:16683-16694.

    Not in our library10.3390/ijms160716683

  57. 57.

    Allan C.R., Hadwiger L.A. The fungicidal effect of chitosan on fungi of varying cell composition. Exp. Mycol. 1979;3:285-287.

    Not in our library10.1016/s0147-5975(79)80054-7

  58. 58.

    Meng X.H., Yang L.Y., Kennedy J.F., Tian S.P. Effects of chitosan and oligochitosan on growth of two fungal pathogens and physiological properties in pear fruit. Carbohydr. Polym. 2010;81:70-75.

    Not in our library10.1016/j.carbpol.2010.01.057

  59. 59.

    Reglinski T., Elmer P.A.G., Taylor J.T., Wood P.N., Hoyte S.M. Inhibition of Botrytis cinerea growth and suppression of botrytis bunch rot in grapes using chitosan. Plant Pathol. 2010;59:882-890.

    Not in our library10.1111/j.1365-3059.2010.02312.x

  60. 60.

    Liu H., Tian W.X., Li B., Wu G.X., Ibrahim M., Tao Z.Y., Wang Y.L., Xie G.L., Li H.Y., Sun G.C. Antifungal effect and mechanism of chitosan against the rice sheath blight pathogen, Rhizoctonia solani. Biotechnol. Lett. 2012;34:2291-2298.

    Not in our library10.1007/s10529-012-1035-z

  61. 61.

    Berger L.R.R., Stamford N.P., Willadino L.G., Laranjeira D., de Lima M.A.B., Malheiros S.M.M., de Oliveira W.J., Stamford T.C.M. Cowpea resistance induced against Fusarium oxysporum f. sp. tracheiphilum by crustaceous chitosan and by biomass and chitosan obtained from Cunninghamella elegans. Biol. Control. 2016;92:45-54.

    Not in our library10.1016/j.biocontrol.2015.09.006

  62. 62.

    Shao X., Cao B., Xu F., Xie S., Yu D., Wang H. Effect of postharvest application of chitosan combined with clove oil against citrus green mold. Postharvest Biol. Technol. 2015;99:37-43.

    Not in our library10.1016/j.postharvbio.2014.07.014

  63. 63.

    Waewthongraka W., Pisuchpen S., Leelasuphakul W. Effect of Bacillus subtilis and chitosan applications on green mold (Penicilium digitatum Sacc.) decay in citrus fruit. Postharvest Biol. Technol. 2015;99:44-49.

    Not in our library10.1016/j.postharvbio.2014.07.016

  64. 64.

    Zahid N., Maqbool M., Siddiqui Y., Manickam S., Ali A. Regulation of inducible enzymes and suppression of anthracnose using submicron chitosan dispersions. Sci. Hortic. 2015;193:381-388.

    Not in our library10.1016/j.scienta.2015.07.014

  65. 65.

    Ali A., Noh N.M., Mustafa M.A. Antimicrobial activity of chitosan enriched with lemongrass oil against anthracnose of bell pepper. Food Pack. Shel. Life. 2015;3:56-61.

    Not in our library10.1016/j.fpsl.2014.10.003

  66. 66.

    Olicón-Hernández D.R., Hernández-Lauzardo A.N., Pardo J.P., Peña A., Velázquez-del Valle M.G., Guerra-Sánchez G. Influence of chitosan and its derivatives on cell development and physiology of Ustilago maydis. Int. J. Biol. Macromol. 2015;79:654-660.

    Not in our library10.1016/j.ijbiomac.2015.05.057

  67. 67.

    Aleksandrowicz-Trzcińska M., Bogusiewicz A., Szkop M., Drozdowski S. Effect of chitosan on disease control and growth of Scots Pine (Pinus sylvestris L.) in a forest nursery. Forests. 2015;6:3165-3176.

    Not in our library10.3390/f6093165

  68. 68.

    Terrile M.C., Mansilla A.Y., Albertengo L., Rodríguez M.S., Anahí Casalongué C. Nitric-oxide-mediated cell death is triggered by chitosan in Fusarium eumartii spores. Pest Manag. Sci. 2015;71:668-674.

    Not in our library10.1002/ps.3814

  69. 69.

    Li B., Wang X., Chen R.X., Huangfu W.G., Xie G.L. Antibacterial activity of chitosan solution against Xanthomonas pathogenic bacteria isolated from Euphorbia pulcherrima. Carbohyd. Polym. 2008;72:287-292.

    Not in our library10.1016/j.carbpol.2007.08.012

  70. 70.

    Mansilla A.Y., Albertengo L., Rodríguez M.S., Debbaudt A., Zúñiga A., Casalongué C.A. Evidence on antimicrobial properties and mode of action of a chitosan obtained from crustacean exoskeletons on Pseudomonas syringae pv. tomato DC3000. Appl. Microbiol. Biotechnol. 2013;97:6957-6966.

    Not in our library10.1007/s00253-013-4993-8

  71. 71.

    Goñi M.G., Tomadoni B., Roura S.I., Moreira M.R. Effect of preharvest application of chitosan and tea tree essential oil on postharvest evolution of lettuce native microflora and exogenous Escherichia coli O157:H7. J. Food Safe. 2014;34:353-360.

    Not in our library10.1111/jfs.12135

  72. 72.

    Li B., Shi Y., Shan C.L., Zhou Q., Ibrahim M., Wang Y.L., Wu G.X., Li H.Y., Xie G.L., Sun G.C. Effect of chitosan solution on the inhibition of Acidovorax citrulli causing bacterial fruit blotch of watermelon. J. Sci. Food Agric. 2013;93:1010-1015.

    Not in our library10.1002/jsfa.5812

  73. 73.

    Badawy M.E., Rabea E.I., Taktak N.E. Antimicrobial and inhibitory enzyme activity of N-(benzyl) and quaternary N-(benzyl) chitosan derivatives on plant pathogens. Carbohydr. Polym. 2014;111:670-682.

    Not in our library10.1016/j.carbpol.2014.04.098

  74. 74.

    Xing K., Zhu X., Peng X., Qin S. Chitosan antimicrobial and eliciting properties for pest control in agriculture: A review. Agron. Sustain. Dev. 2015;35:569-588.

    Not in our library10.1007/s13593-014-0252-3

  75. 75.

    Kulikov S.N., Chirkov S.N., Il’ina A.V., Lopatin S.A., Varlamov V.P. Effect of molecular weight of chitosan on its antiviral activity in plants. Appl. Biochem. Microbiol. 2006;42:200-203.

    Not in our library10.1134/s0003683806020165

  76. 76.

    Davydova V.N., Nagorskaya V.P., Gorbach V.I., Kalitnik A.A., Reunov A.V., Solov’eva T.F., Ermak I.M. Chitosan antiviral activity: Dependence on structure and depolymerization method. Appl. Biochem. Microb. 2011;47:103-108.

    Not in our library10.1134/s0003683811010042

  77. 77.

    Badawy M.E.I., Ahmed F. El-Aswad, A.F. Insecticidal activity of chitosans of different molecular weights and chitosan-metal complexes against cotton leafworm Spodoptera littoralis and oleander aphid Aphis nerii. Plant Protect. Sci. 2012;48:131-141.

    Not in our library

  78. 78.

    El-Sayed S.M., Mahdy M.E. Effect of chitosan on root-knot nematode, Meloidogyne javanica on tomato plants. Int. J. ChemTech. Res. 2015;7:1985-1992.

    Not in our library

  79. 79.

    Cooper W.R., Horton D.R. Effects of elicitors of host plant defenses on pear psylla, Cacopsylla pyricola. Entomol. Exp. Appl. 2015;157:300-306.

    Not in our library10.1111/eea.12360

  80. 80.

    Tan X.L., Zhao J., Wang S., Zang F. Optimization and evaluation of microencapsulated artificial diet for mass rearing the predatory ladybird Propylea japonica (Coleoptera: Coccinellidae). Insect Sci. 2015;22:111-120.

    Not in our library10.1111/1744-7917.12098

  81. 81.

    Sathiyabama M., Akila G., Einstein Charles R. Chitosan-induced defence responses in tomato plants against early blight disease caused by Alternaria solani (Ellis and Martin) Sorauer. Arch. Phytopathol. Plant Prot. 2014;47:1777-1787.

    Not in our library10.1080/03235408.2013.858423

  82. 82.

    Mondal M.M.A., Malek M.A., Puteh A.B., Ismail M.R., Ashrafuzzaman M., Naher L. Effect of foliar application of chitosan on growth and yield in okra. Aust. J. Crop Sci. 2012;6:918-921.

    Not in our library

  83. 83.

    Mathew R., Sankar P.D. Effect of methyl jasmonate and chitosan on growth characteristics of Ocimum basilicum L., Ocimum sanctum L. and Ocimum gratissimum L. cell suspension cultures. Afr. J. Biotechnol. 2012;11:4759-4766.

    Not in our library

  84. 84.

    Chookhongkha N., Miyagawa S., Jirakiattikul Y., Photchanachai S. Chili growth and seed productivity as affected by chitosan. Proceedings of the International Conference on Agriculture Technology and Food Sciences (ICATFS’2012). 146-149.

    Not in our library

  85. 85.

    Wang M., Chen Y., Zhang R., Wang W., Zhao X., Du Y., Yin H. Effects of chitosan oligosaccharides on the yield components and production quality of different wheat cultivars (Triticum aestivum L.) in Northwest China. Field Crops Res. 2015;172:11-20.

    Not in our library10.1016/j.fcr.2014.12.007

  86. 86.

    Ahmadi B., Shariatpanahi M.E. Proline and chitosan enhanced efficiency of microspore embryogenesis induction and plantlet regeneration in Brassica napus L. Plant Cell Tiss. Org. Cult. 2015;123:57-65.

    Not in our library10.1007/s11240-015-0814-3

  87. 87.

    Corsi B., Riccioni L., Forni C. In vitro cultures of Actinidia deliciosa (A. Chev) C.F. Liang & A.R. Ferguson: A tool to study the SAR induction of chitosan treatment. Org. Agric. 2015;5:189-198.

    Not in our library

  88. 88.

    Kananont N., Pichyangkura R., Chanprame S., Chadchawan S., Limpanavech P. Chitosan specificity for the in vitro seed germination of two Dendrobium orchids (Asparagales: Orchidaceae). Sci. Hortic. 2010;124:239-247.

    Not in our library10.1016/j.scienta.2009.11.019

  89. 89.

    Ramos-García M., Ortega-Centeno S., Hernández-Lauzardo A.N., Alia-Tejacal I., Bosquez-Molina E., Bautista-Baños S. Response of gladiolus (Gladiolus spp.) plants after exposure corms to chitosan and hot water treatments. Sci. Hortic. 2009;121:480-484.

    Not in our library10.1016/j.scienta.2009.03.002

  90. 90.

    Salachna P., Zawadzińska A. Effect of chitosan on plant growth, flowering and corms yield of potted freesia. J. Ecol. Eng. 2014;15:97-102.

    Not in our library

  91. 91.

    Jing H.J., Li H.Q. Chitooligosaccharide prolongs vase life of cut roses by decreasing reactive oxygen species. Kor. J. Hort. Sci. Technol. 2015;33:383-389.

    Not in our library10.7235/hort.2015.14188

  92. 92.

    Van S.N., Minh H.D., Anh D.N. Study on chitosan nanoparticles on biophysical characteristics and growth of Robusta coffee in green house. Biocatal. Agric. Biotechnol. 2013;2:289-294.

    Not in our library

  93. 93.

    El-Miniawy S., Ragab M., Youssef S., Metwally A. Response of strawberry plants to foliar spraying of chitosan. Res. J. Agric. Biol. Sci. 2013;9:366-372.

    Not in our library

  94. 94.

    Górnik K., Grzesik M., Romanowska-Duda B. The effect of chitosan on rooting of grapevine cuttings and on subsequent plant growth under drought and temperature stress. J. Fruit Ornam. Plant Res. 2008;16:333-343.

    Not in our library

  95. 95.

    Saavedra G.M., Figueroa N.E., Poblete L.A., Cherian S., Figueroa C.R. Effects of preharvest applications of methyl jasmonate and chitosan on postharvest decay, quality and chemical attributes of Fragaria chiloensis fruit. Food Chem. 2016;190:448-453.

    Not in our library10.1016/j.foodchem.2015.05.107

  96. 96.

    Sathiyabama M., Charles R.E. Fungal cell wall polymer based nanoparticles in protection of tomato plants from wilt disease caused by Fusarium oxysporum f.sp. lycopersici. Carbohydr. Polym. 2015;133:400-407.

    Not in our library10.1016/j.carbpol.2015.07.066

  97. 97.

    Kerch G. Chitosan films and coatings prevent losses of fresh fruit nutritional quality: A review. Trends Food Sci. Technol. 2015;46:159-166.

    Not in our library10.1016/j.tifs.2015.10.010

  98. 98.

    Hewajulige I.G.N., Wilson Wijeratnam R.S., Perera M.G.D.S., Fernando S.A. Extending storage life of commercially important tropical fruits using bio-waxes. Acta Horticul. 2015;1091:283-290.

    Not in our library10.17660/actahortic.2015.1091.35

  99. 99.

    Tayel A.A., Moussa S.H., Salem M.F., Mazrou K.E., El-Tras W.F. Control of citrus molds using bioactive coatings incorporated with fungal chitosan/plant extracts composite. J. Sci. Food Agric. 2016;96:1306-1312.

    Not in our library10.1002/jsfa.7223

  100. 100.

    Kayaa M., Cesoniene L., Daubaras R., Leskauskaite D., Zabulione D. Chitosan coating of red kiwifruit (Actinidia melanandra) for extending of the shelf life. Int. J. Biol. Macromol. 2016;85:355-360.

    Not in our library10.1016/j.ijbiomac.2016.01.012

  101. 101.

    Carvalho R.L., Cabral M.F., Germano T.A., de Carvalho W.M., Brasil I.M., Gallão M.I., Moura C.F.H., Lopes M.M.A., de Miranda M.R.A. Chitosan coating with trans-cinnamaldehyde improves structural integrity and antioxidant metabolism of fresh-cut melon. Postharv. Biol. Technol. 2016;113:29-39.

    Not in our library10.1016/j.postharvbio.2015.11.004

  102. 102.

    Petriccione M., Pasquariello M.S., Mastrobuoni F., Zampella L., Di Patre D., Scortichini M. Influence of a chitosan coating on the quality and nutraceutical traits of loquat fruit during postharvest life. Sci. Hortic. 2015;197:287-296.

    Not in our library10.1016/j.scienta.2015.09.051

  103. 103.

    Zhang Y., Zhang M., Yang H. Postharvest chitosan-g-salicylic acid application alleviates chilling injury and preserves cucumber fruit quality during cold storage. Food Chem. 2015;174:558-563.

    Not in our library10.1016/j.foodchem.2014.11.106

  104. 104.

    FAO Statistical Yearbook 2013.

    Not in our library

  105. 105.

    Sun B., Zhang L., Yang L., Zhang F., Norse D., Zhu Z. Agricultural non-point source pollution in China: Causes and mitigation measures. Ambio. 2012;41:370-379.

    Not in our library10.1007/s13280-012-0249-6

  106. 106.

    Ghormade V., Deshpande M.V., Paknikar K.M. Perspectives for nano-biotechnology enabled protection and nutrition of plants. Biotechnol. Adv. 2011;29:792-803.

    Not in our library10.1016/j.biotechadv.2011.06.007

  107. 107.

    Khot L.R., Sankaran S., Maja J.M., Ehsani R., Schuster E.W. Applications of nanomaterials in agricultural production and crop protection: A review. Crop Prot. 2012;35:64-70.

    Not in our library10.1016/j.cropro.2012.01.007

  108. 108.

    Kah M., Hofmann T. Nanopesticide research: Current trends and future priorities. Environ. Int. 2014;63:224-235.

    Not in our library10.1016/j.envint.2013.11.015

  109. 109.

    Handford C.E., Dean M., Henchion M., Spence M., Elliott C.T., Campbell K. Implications of nanotechnology for the agri-food industry: Opportunities, benefits and risks. Trends Food Sci. Technol. 2014;40:226-241.

    Not in our library10.1016/j.tifs.2014.09.007

  110. 110.

    Cota-Arriola O., Cortez-Rocha M.O., Burgos-Hernández A., Ezquerra-Brauer J.M., Plascencia-Jatomea M. Controlled release matrices and micro/nanoparticles of chitosan with antimicrobial potential: Development of new strategies for microbial control in agriculture. J. Sci. Food Agric. 2013;93:1525-1536.

    Not in our library10.1002/jsfa.6060

  111. 111.

    Kah M., Tiede K., Beulke S., Hofmann T. Nanopesticides: State of knowledge, environmental fate, and exposure modeling. Crit. Rev. Environ. Sci. Technol. 2013;43:1823-1867.

    Not in our library10.1080/10643389.2012.671750

  112. 112.

    Quiñones J.P., García Y.C., Curiel H., Covas C.P. Microspheres of chitosan for controlled delivery of brassinosteroids with biological activity as agrochemicals. Carbohydr. Polym. 2010;80:915-921.

    Not in our library10.1016/j.carbpol.2010.01.006

  113. 113.

    Lao S.B., Zhang Z.X., Xu H.H., Jiang G.B. Novel amphiphilic chitosan derivatives: Synthesis, characterization and micellar solubilization of rotenone. Carbohydr. Polym. 2010;82:1136-1142.

    Not in our library10.1016/j.carbpol.2010.06.044

  114. 114.

    Feng B.H., Peng L.F. Synthesis and characterization of carboxymethyl chitosan carrying ricinoleic functions as an emulsifier for azadirachtin. Carbohydr. Polym. 2012;88:576-582.

    Not in our library10.1016/j.carbpol.2012.01.002

  115. 115.

    Paula H.C.B., Sombra F.M., Cavalcante R.F., Abreu F.O.M.S., de Paula R.C.M. Preparation and characterization of chitosan/cashew gum beads loaded with Lippia sidoides essential oil. Mater. Sci. Eng. C. 2011;31:173-178.

    Not in our library10.1016/j.msec.2010.08.013

  116. 116.

    Guan H., Chi D., Yu J., Li X. A novel photodegradable insecticide: Preparation, characterization and properties evaluation of nano-Imidacloprid. Pest. Biochem. Physiol. 2008;92:83-91.

    Not in our library10.1016/j.pestbp.2008.06.008

  117. 117.

    Manikandan A., Sathiyabama M. Preparation of chitosan nanoparticles and its effect on detached rice leaves infected with Pyricularia grisea. Int. J. Biol. Macromol. 2016;84:58-61.

    Not in our library10.1016/j.ijbiomac.2015.11.083

  118. 118.

    Xing K., Shen X., Zhu X., Ju X., Miao X., Tian J., Feng Z., Peng X., Jiang J., Qin S. Synthesis and in vitro antifungal efficacy of oleoyl-chitosan nanoparticles against plant pathogenic fungi. Int. J. Biol. Macromol. 2016;82:830-836.

    Not in our library10.1016/j.ijbiomac.2015.09.074

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    Davidson D., Gu F.X. Materials for sustained and controlled release of nutrients and molecules to support plant growth. J. Agric. Food Chem. 2012;60:870-876.

    Not in our library10.1021/jf204092h

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    Wu L., Liu M., Liang R. Preparation and properties of a double-coated slow-release NPK compound fertilizer with superabsorbent and water-retention. Bioresour. Technol. 2008;99:547-554.

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    A preliminary study of the incorparation of NPK fertilizer into chitosan nanoparticles · 2010
    Not checked
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    Hussain M.R., Devi R.R., Maji T.K. Controlled release of urea from chitosan microspheres prepared by emulsification and cross-linking method. Iran. Polym. J. 2012;21:473-479.

    Not in our library10.1007/s13726-012-0051-0

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    Santos B.R.D., Bacalhau F.B., Pereira T.D.S., Souza C.F., Faez R. Chitosan-Montmorillonite microspheres: A sustainable fertilizer delivery system. Carbohydr. Polym. 2015;127:340-346.

    Not in our library10.1016/j.carbpol.2015.03.064

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    Narayanan A., Dhamodharan R. Super water-absorbing new material from chitosan, EDTA and urea. Carbohydr. Polym. 2015;134:337-343.

    Not in our library10.1016/j.carbpol.2015.08.010

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    Alloway B., Alloway B.J. Micronutrient Deficiencies in Global Crop Production. 2008;1-39.

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    Martens D.C., Westermann D.T. Micronutrients in Agriculture. 1991;549-592.

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    Peteu S.F., Oancea F., Sicuia O.A., Constantinescu F., Dinu S. Responsive polymers for crop protection. Polymers. 2010;2:229-251.

    Not in our library10.3390/polym2030229

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    Chen C., Gao Z., Qiu X., Hu S. Enhancement of the controlled-release properties of chitosan membranes by crosslinking with suberoyl chloride. Molecules. 2013;18:7239-7252.

    Not in our library10.3390/molecules18067239

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    Tao S., Pang R., Chen C., Ren X., Hu S. Synthesis, characterization and slow release properties of O-naphthylacetyl chitosan. Carbohydr. Polym. 2012;88:1189-1194.

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    Pundir C.S., Chauhan N. Acetylcholinesterase inhibition-based biosensors for pesticide determination: A review. Anal. Biochem. 2012;429:19-31.

    Not in our library10.1016/j.ab.2012.06.025

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    Silva M.S., Cocenza D.S., Grillo R., de Melo N.F.S., Tonello P.O.S., deOliveira L.C., Cassimiro D.L., Rosa A.H., Fraceto L.F. Paraquat-loaded alginate/chitosan nanoparticles: Preparation, characterization and soil sorption studies. J. Hazard. Mater. 2011;190:366-374.

    Not in our library10.1016/j.jhazmat.2011.03.057

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    Grillo R., Pereira A.E.S., Nishisaka C.S., de Lima R., Oehlke K., Greiner R., Fraceto L.F. Chitosan/tripolyphosphate nanoparticles loaded with paraquat herbicide: An environmentally safer alternative for weed control. J. Hazard. Mater. 2014;278:163-171.

    Not in our library10.1016/j.jhazmat.2014.05.079

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    Namasivayam S.K.R., Aruna A., Gokila G. Evaluation of silver nanoparticles-chitosan encapsulated synthetic herbicide paraquat (AgNp-CS-PQ) preparation for the controlled release and improved herbicidal activity against Eichhornia crassipes. Res. J. Biotechnol. 2014;9:19-27.

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  134. 134.

    Celis R., Adelino M.A., Hermosín M.C., Cornejo J. Montmorillonite–chitosan bionanocomposites as adsorbents of the herbicide clopyralid in aqueous solution and soil/water suspensions. J. Hazard. Mater. 2012;209–210:67-76.

    Not in our library10.1016/j.jhazmat.2011.12.074

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    Wen Y.Z., Chen H., Yuan Y.L., Xu D.M., Kang X.D. Enantioselective ecotoxicity of the herbicide dichlorprop and complexes formed with chitosan in two fresh water green algae. J. Environ. Monit. 2011;13:879-885.

    Not in our library10.1039/c0em00593b

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    Bao J., Hou C., Chen M., Li J., Huo D., Yang M., Luo X., Lei Y. Plant esterase–chitosan/gold nanoparticles–graphene nanosheet composite-based biosensor for the ultrasensitive detection of organophosphate pesticides. J. Agric. Food Chem. 2015;63:10319-10326.

    Not in our library10.1021/acs.jafc.5b03971

  137. 137.

    Sargın I., Kaya M., Arslan G., Baran T., Ceter T. Preparation and characterisation of biodegradable pollen–chitosan microcapsules and its application in heavy metal removal. Biores.Technol. 2015;177:1-7.

    Not in our library10.1016/j.biortech.2014.11.067

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    Sargın I., Arslan G., Kaya M. Microfungal spores (Ustilago maydis and U. digitariae) immobilized chitosan microcapsules for heavy metal removal. Carbohydr. Polym. 2016;138:201-209.

    Not in our library10.1016/j.carbpol.2015.11.065

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    Krenek P., Samajova O., Luptovciak I., Doskocilova A., Komis G., Samaj J. Transient plant transformation mediated by Agrobacterium tumefaciens: Principles, methods and applications. Biothecnol. Adv. 2015;33:1024-1042.

    Not in our library10.1016/j.biotechadv.2015.03.012

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    Mao S., Sun W., Kissel T. Chitosan-based formulations for delivery of DNA and siRNA. Adv. Drug Deliv. Rev. 2010;62:12-27.

    Not in our library10.1016/j.addr.2009.08.004

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    Wang Q., Chen J.N., Zhan P., Zhang L., Kong Q.Q. Establishment of a suspension cell system for transformation of Jatropha curcas using nanoparticles. Adv. Mater. Res. 2013;608–609:314-319.

    Not in our library10.4028/www.scientific.net/amr.690-693.314

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    Wong M.H., Misra R.P., Giraldo J.P., Kwak S.Y., Son Y., Landry M.P., Swan J.W., Blankschtein D., Strano M.S. Lipid exchange envelope penetration (LEEP) of nanoparticles for plant engineering: A universal localization mechanism. Nano Lett. 2016;16:1161-1172.

    Not in our library10.1021/acs.nanolett.5b04467

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