The Synthesis and Role of β-Alanine in Plants

1β-Alanine is a non-proteinogenic amino acid, where the amino group is at the β-position from the carboxylate group (IUPAC name = 3-aminopropanoic acid). In contrast to L-alanine, which is a proteinogenic amino acid, β-alanine has no stereo center (Figure 1). β-Alanine is incorporated into pantothenate (Vitamin B5), and therefore, is a precursor of Coenzyme A (CoA) and acyl-carrier protein, which shuttle carbon within the cell. β-Alanine is a component of carnosine, a dipeptide concentrated in muscle and brain tissue, which underlies the wide use of β-alanine in humans as a strength enhancing supplement. The vast majority of scientific articles about β-alanine deal with the exercise supplement aspect, please see for example. Much less is known about the role of β-alanine in plants. The focus of this manuscript is the current state of knowledge on the metabolism and role of β-alanine in plants.

2Apart from the universal importance of β-alanine (as the precursor of CoA) in the synthesis of phospholipids, synthesis and degradation of fatty acids, and the operation of the tricarboxylic acid cycle, plants also employ β-alanine in secondary metabolism, including lignin biosynthesis. Moreover, β-alanine is involved in multiple stress responses in plants. LeSvels of β-alanine have been shown to be elevated significantly following heat shock in Vigna unguiculata cell cultures. Various biotic and abiotic stresses on Medicago truncatula cell cultures resulted in elevated β-alanine levels, suggesting changes in the metabolism of CoA and its thioesters, which are essential in secondary metabolism. Both drought and heat stress were found to induce increased β-alanine levels in the model plant Arabidopsis thaliana. Metabolomics studies reveal that in response to cadmium ions (heavy metal stress); A. thaliana accumulates multiple compounds, including L-alanine, β-alanine, and the polyamine putrescine.

3β-Alanine aminotransferases from plants have been known since at least the late 1960s. Work on enzymes from Phaseolus vulgaris (wax bean) cotyledons showed that β-alanine could be converted into the well-known plant signaling molecule ethylene. In some plants, β-alanine is additionally converted to β-alanine betaine, an important quaternary ammonium osmoprotective compound that is involved in tolerance to both high salt concentration and low oxygen. A role for β-alanine in recovery from waterlogging was suggested in an earlier study employing a hydroponic system. In vitro protection against high temperature stress for the enzyme lactate dehydrogenase (LDH) is afforded by β-alanine.

4In some leguminous plants, β-alanine forms a part of the thiol tripeptide homoglutathione, which is involved in protection against heavy metal toxicity and free radicals (reactive oxygen species). For this reason, pathways involving β-alanine are considered attractive targets for the metabolic engineering of plants, potentially including crop plants in a world facing a growing human population and increasing environmental stresses due to climate change. Work that is more recent suggests that β-alanine metabolism in A. thaliana involved in a variety of pathways and roles such as nitrogen utilization efficiency, response to hypoxia, osmoprotection, vitamin B5 and CoA metabolism.

5The biosynthesis of β-alanine in plants may be initiated from at least four different precursors, namely (1) the polyamines spermine/spermidine, (2) the carboxylic acid propionate, (3) the nucleotide base uracil, and (4) the proteinogenic amino acid L-aspartate. Among these, the first three are thoroughly characterized, while the fourth has long been postulated without conclusive experimental evidence. The physiological implications of each pathway are discussed below.

6The polyamines spermine and spermidine are converted via 1,3-diaminopropane to β-alanine (Figure 2) in many plants and demonstrated in maize shoots. Pericarp discs of tomato fruits were also shown to degrade spermidine to putrescine and β-alanine. Spermidine is cleaved by the FAD- and heme-containing enzyme spermidine dehydrogenase [EC 1.5.99.6] into 1,3-diaminopropane and 4-aminobutyraldehyde. Spermine degradation is facilitated by the incorporation of water and molecular oxygen by the enzyme polyamine oxidase [EC 1.5.3.14], yielding 1,3-diaminopropane, 4-aminobutyraldehyde, and hydrogen peroxide. The common intermediate 1,3-diaminopropane is then deaminated with the addition of water and molecular oxygen into 3-aminopropionaldehyde, hydrogen peroxide, and ammonia by the enzyme diamine oxidase [EC 1.4.3.22]. Finally, oxidation of 3-aminopropionaldehyde with the addition of water by the NAD(P)-dependent aldehyde dehydrogenase [EC 1.2.1.3] yields β-alanine. Spermidine oxidation via these enzymes is induced by abiotic stresses.

7AtALDH10A8, an NAD+-dependent aminoaldehyde dehydrogenase [EC 1.2.1.19] in A. thaliana was shown to convert 3-aminopropionaldehyde into β-alanine in vitro, and similar enzymes were studied in apple fruit. However, unlike gamma-aminobutyric acid (GABA), which was specifically accumulated in response to salinity, β-alanine was not, suggesting that β-alanine is accumulated as part of a non-specific rather than a specific stress response. A. thaliana has been shown to survive without the ability to make spermine, but spermidine synthesis is essential to survival. This suggests that spermidine (but not spermine) could be linked to pantothenate biosynthesis via β-alanine. The condensation of β-alanine with pantoate in plants (Figure 2) yields pantothenate (vitamin B5), which is essential for all organisms as a precursor to the 4′-phosphopantetheine moiety of coenzyme A (CoA) and acyl carrier protein.

8As seen in Figure 2, there is an intriguing link between the metabolism of the branched chain amino acids (BCAA) and β-alanine. It appears that there is a feedback loop whereby a change in the levels of β-alanine may be effected via BCAA degradation. Since valine and isoleucine may serve as precursors for propionyl-CoA, recent studies in this regard are discussed in the next section.

9Propionate may act as the precursor for β-alanine in the pathway involving a β-alanine aminotransferase (Figure 3). Propionate is activated with the hydrolysis of ATP to propionyl-CoA A by propionate CoA ligase [EC 6.2.1.17]. Propionyl-CoA is then oxidized to acrylyl-CoA by the FAD-dependent medium chain acyl-CoA dehydrogenase [EC 1.3.8.7]. 3-hydroxypropionyl-CoA dehydratase [EC 4.2.1.116], then hydrates acrylyl-CoA yielding 3-hydroxypropionyl-CoA. Hydrolysis of the CoA ester by 3-hydroxypropionyl-CoA hydrolase [EC 3.1.2.4] generates 3-hydroxyproiponate, which is further oxidized to malonate semialdehyde by the NAD-dependent 3-hydroxypropionate dehydrogenase [EC 1.1.1.59]. The final step in this pathway is the transamination catalyzed by the pydridoxal-5′-phosphate (PLP)-dependent β-alanine-pyruvate transaminase [EC 2.6.1.18], whereby an amino group is transferred from L-alanine to malonate semialdehyde, yielding β-alanine and pyruvate. After 2001 study identified a β-alanine aminotransferase in A. thaliana, it was hypothesized that this pathway may be involved in pantothenate production. In A. thaliana, there is a gene encoding for the pantothenate synthase (PtS) enzyme, which condenses pantoate with β-alanine to yield pantothenate. It should be noted that homologous gene/s encoding aspartate decarboxylase (ADC), the enzyme that generates β-alanine in the bacterial pantothenate pathway were not identified in the plant.

10The propionate pathway connects the metabolism of L-alanine and β-alanine in plants, since the final step requires the former amino acid as the amino donor (Figure 3). Therefore, a change in the levels of one or the other of the two amino acids may have wide-ranging physiological effects. Gene regulation studies of alanine aminotransferase has been studied in many plants in response to low-oxygen stress, light, and nitrogen, and it was demonstrated that hypoxia induced the expression of two distinct alanine aminotransferase genes in A. thaliana. The function of the gene product from the locus tag At3g08860 was unknown for a long time, but was recently shown to encode a β-alanine/L-alanine aminotransferase using an in vivo functional complementation approach. The same study reported that a definitive preference of the corresponding enzymatic activity toward the synthesis of β-alanine. The gene corresponding to the β-alanine/L-alanine aminotransferase was shown to be most highly expressed in A. thaliana roots. The enrichment of the β-alanine aminotransferase enzyme in the roots suggests that β-alanine possibly has a specific protective function in the root tissue. Earlier, hydroponics studies in Helianthus annuus L. revealed that roots which were excised and flooded for 24 h had reduced uptake of radioactive β-alanine than non-flooded roots and also that they exuded more radioactive β-alanine into distilled water. This suggests, first, that there is a regulation of β-alanine uptake (if available in the medium), and second, that flooded roots may exude β-alanine for protective purposes. Therefore, it is likely that plants require enhanced expression of the β-alanine aminotransferase in the roots to facilitate a β-alanine based defense response when faced with hypoxia or flooding.

11Additional studies have addressed the links between branched-chain amino acids and β-alanine, since valine and isoleucine degradation can produce propionyl-CoA. Using radiolabeled precursors of both isoleucine and valine, it was shown in A. thaliana that only isoleucine directly generated β-alanine. However, if seedlings were treated with valine, an increase in the levels of β-alanine resulted, suggesting an indirect effect via amino acid homeostasis. Seedlings harboring a mutated methylmalonate semialdehyde dehydrogenase (MMSD, At2g14170) gene cannot convert valine into propionyl-CoA (since the Mmsd-1 protein catalyzes the final step leading to propionyl-CoA); but the equilibrium is shifted toward β-alanine, presumably by the amino acid homeostatic mechanism. More recently, feeding of 13C-labeled isoleucine and propionate were shown to result in the production of C13-labeled 3-hydroxypropionate and β-alanine in A. thaliana seedlings, offering direct evidence for the metabolic link between isoleucine and β-alanine via propionyl-CoA. Further studies in transgenic A. thaliana and wheat seedlings confirmed that isoleucine degradation could initiate β-alanine synthesis. This suggests that isoleucine may serve as an additional precursor for β-alanine in plants (apart from those known already) and since valine is a precursor of pantoate, pantothenate production could depend on BCAA catabolism.

12The nucleotide base uracil is reduced by NADPH-dependent dihydrouracil dehydrogenase [EC 1.3.1.2] into dihydrouracil (Figure 4A); in A. thaliana, this gene is identified with the At3g17810 locus. The hydrolysis of dihydrouracil into 3-ureidopropionate is catalyzed by dihydropyrimidinase [EC 3.5.2.2] (Figure 4A); the accession IDs in A. thaliana are At5g12200 and At5g12200.1. Further hydrolysis by β-ureidopropionase [EC 3.5.1.6] eliminates ammonia and carbon dioxide, yielding β-alanine as shown in Figure 4A; the A. thaliana accession IDs are At5g64370 and At5g64370.1. The degradation of uracil and thymine to produce carbon dioxide, ammonia, β-alanine, and γ-aminoisobutyrate has been shown to occur in seedlings of Brassica napus (rapeseed), while the β-ureidopropionase enzyme of maize was characterized by its overexpression in Escherichia coli. The existence of this pathway in pine trees was inferred already in the 1960s. In the halotolerant members of the leadwort or plumbago family (Plumbaginaceae) of perennial plants, uracil is only one of many sources for β-alanine. Thus, the distribution of this pathway in higher plants varies widely.

13L-aspartate may be decarboxylated by aspartate 1-decarboxylase [EC 4.1.1.11] also yielding β-alanine, which may enter Coenzyme A biosynthesis (Figure 4B). This enzyme abbreviated ADC and encoded by the panD gene has been well characterized in prokaryotes. However, its presence in plants has never been identified and or confirmed. Bioinformatics analysis based on sequence homology using the FUGUE tool failed to find homologs in either A. thaliana or the yeast Saccharomyces cerevisiae genomes, casting doubt on whether this pathway crossed the divide between prokaryotes and eukaryotes. With the availability of numerous genome sequences of plants and fungi available since 2004, further inquiry of panD orthologs using bioinformatics tools is warranted.

14Since only plants and microbes synthesize pantothenate de novo, there is significant interest in engineering this pathway in plants in order to fortify food crops with pantothenate, which is essential for animals since they lack the enzymes required to biosynthesize the compound. Also, since only plants and microbes contain the pathway for pantothenate, it is also considered an attractive target for herbicides, fungicides and antibiotic development. Increased heat tolerance via overproduction of β-alanine and pantothenate content could be achieved in transgenic tobacco plants harboring the gene for the E. coli ADC enzyme. Later, the same system was also shown to enhance photosynthesis and to augment biomass production in response to higher temperatures. These are important practical advances that could pave the way for heat resistant transgenic food crops that are better suited in warmer climate zones and in changing climates, where loss in agricultural productivity with increasing temperatures might be a concern.

15β-Alanine betaine is an osmoprotective compound accumulated by most members of the highly stress-tolerant leadwort or plumbago family (Plumbaginaceae). β-Alanine betaine is synthesized by S-adenosyl-L-methionine (SAM)-dependent enzymatic N-methylation of β-alanine (Figure 5A) via N-methyl β-alanine and N,N-dimethyl β-alanine. Most members of the highly stress-tolerant Plumbaginaceae accumulate β-alanine betaine instead of glycine betaine. It was proposed that β-alanine betaine is a more suitable osmoprotectant than glycine betaine under saline hypoxic conditions because the first step in glycine betaine synthesis catalyzed by choline monooxygenase [EC 1.14.15.7] requires molecular oxygen. Further, β-alanine betaine accumulation was proposed to be an evolutionary strategy to avoid the metabolic limitations existing for choline oxidation under hypoxic conditions, because β-alanine betaine is synthesized from the ubiquitous primary metabolite β-alanine.

16In many legumes, the thiol tripeptide homoglutathione (hGSH; γGlu-Cys-βAla) (Figure 5B) can partially or fully replace the better-known thiol, glutathione. γ-Glu-Cys is formed from L-glutamate and L-cysteine by glutamate cysteine ligase (γ-Glutamylcysteine synthetase or γ-ECS) at the expense of ATP, and γ-Glu-Cys and β-alanine are condensed at the expense of ATP by a specific hGSH synthetase (hGSHS), an enzyme which has affinity for β-alanine and low affinity for glycine. Multiple cellular compartments are involved in the biosynthesis of hGSH and fractionation of the root nodules demonstrated that the bacteroids contain high hGSH concentrations and the highest specific activities of GSHS, suggesting a critical role in nitrogen fixation processes in the root nodules of legume plants. More recent work in M. truncatula also showed that hGSH is essential for the growth of parasitic nematode worms, which infect plant roots and force the differentiation of root cells into giant cells. In addition, it was also shown that hGSH-depleted roots did not suffer similar damage by nematodes. Thus, hGSH may play key roles in plant defense and could be the target of future interventions into improving plant resistance to nematodes.

17Although underappreciated, the non-proteinogenic amino acid β-alanine has important roles in plant physiology and metabolism, directly as a defense compound that enables plants to withstand various stresses such as hypoxia, waterlogging and drought, and indirectly as a precursor to the compounds pantothenate and CoA, which are involved in a variety of functions. Furthermore, the amino acid is converted into β-alanine betaine, which has additional protective functions such as salt tolerance, and homoglutathione, which may be critical for nitrogen fixation.

18All authors listed have made a substantial, direct and intellectual contribution to the work, and approved it for publication.

19The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.