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Spatial and Temporal Profile of Glycine Betaine Accumulation in Plants Under Abiotic Stresses

Maria Grazia Annunziata, Loredana Filomena Ciarmiello, Pasqualina Woodrow and 2 more

Frontiers in Plant Science · 2019 · Review · CC BY

doi:10.3389/fpls.2019.00230

Abstract

Several halophytes and a few crop plants, among which Poaceae, synthesize and accumulate glycine betaine (GB) in response to environmental constraints. GB has an important role in osmoregulation, in fact, it is one of the main nitrogen-containing compatible osmolytes found in Poaceae. It can interplay with molecules and structures preserving the activity of macromolecules, maintaining the integrity of membranes against stresses and scavenging ROS. Exogenous GB applications have been proved to induce the expression of genes involved in oxidative stress responses, with a restriction of ROS accumulation and lipid peroxidation in cultured tobacco cells under drought and salinity, even stabilizing photosynthetic structures under stress. In the plant kingdom, GB is synthesized from choline by two-step oxidation reactions. The first oxidation is catalyzed by choline monooxygenase (CMO) and the second oxidation is catalyzed by NAD+-dependent betaine aldehyde dehydrogenase (BADH). Moreover, in plants, the cytosolic enzyme, named N-methyltransferase (PEAMT), catalyzes the conversion of phosphoethanolamine to phosphocholine. However, changes in CMO expression genes under abiotic stresses have been observed. GB accumulation is ontogenetically controlled since it happens in young tissues during prolonged stress, while its degradation is generally not significant in plants. This ability of plants to accumulate high levels of GB in young tissues under abiotic stresses is independent of nitrogen (N) availability and supports the view that plant N allocation is dictated primarily to supply and protect the growing tissues even under N limitation. Indeed, the contribution of GB to osmotic adjustment and ionic and oxidative stress defense in young tissues is much higher than that in older ones. In this review, the biosynthesis and accumulation of GB in plants under several abiotic stresses are analyzed focusing the attention on all the possible roles this metabolite can play, in particular in young tissues.

Full text

From PubMed Central, U.S. National Library of Medicine, as current on Oct 21, 2026. Paragraph numbers are ours, so an answer can point at one.

Glycine Betaine Metabolic Pathways

Diverse halophytes, but only a few crop plants, including Poaceae, synthetize and accumulate glycine betaine (GB) in response to environmental constrains.

In plants, GB synthesis starts from choline, which, in turn, is synthesized through three sequential adenosyl-methionine dependent methylations of phospho-ethanolamine (PE) catalyzed by the cytosolic enzyme phospho-ethanolamine N-methyltransferase (PEAMT; EC 2.1.1.103). The PEAMT enzyme has two methyltransferase domains in tandem at the N and C-terminal domains; the former converting PE into phospho-monomethylethanolamine (P-MME), and the latter methylating P-MME to phospho-dimethylethanolamine (P-DME) and P-DME to phospho-choline. The product of PEAMT is phospho-choline (PC) which in different plants can undergo different pathways for the transformation to choline. In spinach, PC is directly dephosphorylated to choline, while in tobacco it is first included in phosphatidyl-choline and then metabolized to choline. Subsequently, GB is synthesized by two oxidations on choline, via betaine aldehyde, catalyzed by a ferredoxin-dependent choline monooxygenase (CMO; EC 1.14.15.7), and a NAD+-dependent betaine aldehyde dehydrogenase (BADH; EC 1.2.1.8), respectively (Figure 1A). CMO has a Rieske-type [2Fe-2S] active site, in addition to a transit peptide sequence, and it is usually localized in the chloroplast or other subcellular compartments. BADH can be either NAD+ or NADP+ dependent, but in plants it shows higher activity with NAD+. It belongs to the superfamily of aldehyde dehydrogenases, and also has a non-specific action on other aldehyde substrates: this also explain its presence in non-GB accumulating plants or in organs of plants that do not contain GB. BADH is induced by abscisic acid (ABA) in cereals, but neither NaCl, ABA nor turgor reduction seem to be directly involved in the induction, but rather in an unknown signal coming mainly from roots as well as other plant parts.

Chenopodiaceae, such as spinach and sugar beet, have CMO and BADH enzymes localized in the chloroplast stroma. In Hordeum vulgare, a peroxisomal NADPH-dependent CMO is involved in the first step of GB synthesis exerting choline oxidation; while BADH is localized in the cytosol and in the chloroplast.

In animals and many bacteria, a membrane-bound choline dehydrogenase (CHDH; EC 1.1.99.1) catalyzes the oxidation of choline to betaine aldehyde; while the enzyme involved in the second step is BADH again (Figure 1B). In Arthrobacter globiformis and Arthrobacter pascens a soluble choline oxidase (CHO; EC 1.1.3.17), coded by a single gene codA, catalyzes the direct four-electron oxidation of choline to GB, with betaine aldehyde as an intermediate. It contains a covalently linked flavin adenine dinucleotide (FAD), and acts through two hydride-transfer reactions, the two reductions of flavin and the rate-limiting steps (Figure 1C). This enzyme has become relevant due to its biotechnological applications for the metabolic engineering of economically important plants for osmotic stress resistance, and the production of sensors for the determination of choline and derivatives in biological fluids.

Glycine betaine can also undergo a stress-inducible synthesis, since it may be derived from the serine that is synthetized by the (i) non-phosphorylated glycerate pathway, (ii) the phosphorylated phospho-hydroxypyruvate pathway, or iii) the salt-stress-induced photorespiratory glycolate pathway. Serine is the precursor of PE via ethanolamine. In plants, the latter is formed directly by the action of a pyridoxal 5′′-phosphate-dependent L-serine decarboxylase (SDC); while in plants and animals, but not in fungi, it can indirectly be synthetized through a base exchange between serine and existing PE. Thereafter, the phosphorylation of ethanolamine is catalyzed by a choline/ethanolamine kinase (CEK; EC 2.7.1.32).

Salinity, specifically, increases the CMO and BADH gene expression two- to three-fold and, consequently, the corresponding enzyme levels.

identified a CGTCA-motif in the promoter region of Citrullus lanatus CMO and BADH genes that are responsive to methyl jasmonate (MeJA). When C. lanatus cells were activated by MeJA, they synthetized GB even without osmotic stress, and the new cells derived by the activated ones retained a high GB content without previous stress or MeJA activation. This finding suggests that JA signal transduction is involved in GB biosynthesis, which plays a key role in both osmotic stress tolerance and osmotic stress hardening.

However, the interactive effects of simultaneous salinity and other stresses can decrease the expression of CMO and the relative GB production. This is particularly relevant because the step catalyzed by CMO is the rate-limiting one in GB biosynthesis. Moreover, it is important to underline that GB is not actively metabolized, therefore its concentration depends on the control of its synthesis, transport and dilution by growth.

, showed that a transcript in durum wheat, coding a putative CMO-like enzyme with a different Rieske-type motif that showed similarity with the CHO, was isolated in Ruegeria sp., Pseudomonas fluorescens, and Rhodococcus sp. suggesting a possible alternative pathway for the production of GB in durum wheat similar to that operating the direct oxidation of choline to GB.

Genetically Engineered Biosynthesis of GB

Different genera, and even different species within the same genus, accumulate contrasting amounts of GB, and are therefore classified as accumulators and non-accumulators. Natural accumulators of GB accumulate large amounts of GB only under abiotic stresses. Homozygous lines for Bet1 (GB accumulators), that are part of near-isogenic maize lines presenting different GB accumulation capacity, showed a 10–20% higher biomass under salinity than the non-accumulating lines. Therefore, metabolic engineering strategies, aimed at increasing the synthesis and accumulation of GB, have been associated with an amelioration in plant stress tolerance. In particular, important crop species like rice (Oryza sativa), potato (Solanum tuberosum), and tomato (Solanum lycopersicum), which are not able to synthesize and accumulate GB, have been considered as potential targets for metabolic engineering of GB biosynthesis.

Several genes involved in the GB biosynthetic pathway have been isolated, cloned and used to generate transgenic plants, accumulating GB with an enhanced tolerance to abiotic stress. The transformations were quite successful for several plant species, improving plant tolerance to salt, drought and extreme temperatures, notwithstanding the very low amounts of GB accumulated by the engineered plants. Among such engineered plants, those transformed with codA from A. globiformis, encoding the enzyme CHO that catalyzes the direct oxidation of choline to GB, also showed the accumulation of GB directly in the chloroplasts. In particular, a successful transformation with codA was obtained in Arabidopsis thaliana, Brassica chinensis, Brassica juncea, and Brassica napus, O. sativa and even in woody plants such as the Japanese persimmon and Eucalyptus globulus. The plants engineered with codA showed an enhanced tolerance to chilling, freezing, salinity, high temperature, and high light in different growth stages, from seed germination to growth, development and reproductive stages. Likewise, significant success has been achieved by engineering plants with betA, betB or both genes from Escherichia coli encoding CHDH and BADH, respectively, such as in Gossypium hirsutum, Medicago sativa, Nicotiana tabacum. codA-transformed rice plants, showed a GB concentration of 5 and 1 μmol g-1 fresh weight in leaves when the transformation was targeted to the cytosol and chloroplast, respectively. While, the transformation of maize, an accumulator plant, with betA increased GB concentration to about 5.7 μmol g-1 fresh weight, a value higher than that present in wild-type (WT) maize plants under drought stress.

However, the efficacy of GB engineering for important cultured field crops has never been demonstrated. The main reason is that even if the levels of GB in the engineered plants were significantly increased, they were still lower than those of high accumulator species, which range from about 4 to 40 μmol g-1 fresh weight. One possible explanation for this is that choline availability may limit GB accumulation in some plants. In fact, transformed tobacco plants, with a spinach cDNA encoding CMO, showed a very low GB production of about 0.02–0.05 μmol g-1 fresh weight in both control and salt stress conditions, and were able to accumulate large amounts of GB only when choline was supplemented. Moreover, the concentration of endogenous choline did not change significantly in all transgenic plants expressing the codA gene. Its availability does not affect the GB synthesis of all transgenic plants, most probably due to synergism in the demand and supply of choline to chloroplast.

In fact, the cytosolic choline in plants, synthetized in the cytosol or exogenously supplied, needs to be transported to the chloroplast for GB biosynthesis. Therefore, it could be possible that different capacities of plants to synthetize GB, could be also dependent on their diverse ability to transport choline to the chloroplast and not only on its availability in the cytosol. Besides, transformed tobacco plants overexpressing CHO in the chloroplast and supplemented with choline, accumulated GB at only 1 μmol g-1 FW, while Arabidopsis which over expressed CHO in the cytosol and were supplemented with choline, accumulated GB at about 120 μmol g-1 FW. Similarly, CHO-transgenic tomato plants were able to accumulate more GB in the cytosol than in the chloroplast. CHO-transgenic maize and rice were able to accumulate similar amounts of GB in both subcellular compartments, confirming that, independent of choline concentration in the cytosol, its species-specific capacity of transport from the cytosol to the chloroplast, highly affects GB production in the chloroplast and the plants tolerance to stress.

Several plant species engineered to express CMO and/or BADH and which are supplemented with 10 mM betaine aldehyde, are able to synthesize GB in amounts comparable to accumulator plants. Specifically, N. tabacum, O. sativa and Daucus carota, transformed with betB and supplemented with betaine aldehyde, were able to produce 4.6, 6, and 10 μmol g-1 fresh weight GB, demonstrating that a significant increase of GB is achievable.

Recently, a novel gene, GB1, differentially expressed in low and high GB accumulating genotypes of maize, was identified by. Transgenic GB1-maize and soybean lines accumulated GB at concentrations 4–10-fold higher than WT plants. GB1 protein is a member of the Pfam fatty acid hydroxylase superfamily, with a suggested peroxisomal location. Its predicted sequence showed 60% identity as a putative C-4 sterol methyl oxidase from rice. GB1 protein certainly has a main role in the GB accumulation in plants, and can be used as an innovative tool to improve tolerance to abiotic stress in crop plants.

Exogenous GB Applications

Glycine betaine improves growth and survival of plants counteracting metabolic dysfunctions caused by stress. Due to the beneficial effects of GB, numerous experiments of exogenous application of this compatible compound, on low-accumulator and non-accumulator plant species have been done. Recent studies, and related reports have proven its effectiveness in increasing plant tolerance to various stresses (Table 1). Exogenous GB is able to preserve Photosystem II (PSII) and the photosynthetic oxygen evolving complex (OEC) association under salinity in Lycopersicon esculentum, H. vulgare and N. tabacum, and to induce the expression of oxidative stress response genes, decreasing ROS accumulation and lipid peroxidation in cultured tobacco cells under salinity. Application of GB to bread wheat leaves, reduces the accumulation of Na+ and increases the accumulation of K+ and Ca2+, improves leaf water potential, enhances the activities of SOD, CAT and POD and, reduces photoinhibition enhancing growth and yield.

showed that salicylic acid induced GB accumulation in Vigna radiata under salinity, with a consequent increase of glutathione, reduction of ethylene and oxidative stress, and improvement of photosynthesis. GB also protects photosynthesis by modifying the lipid composition of the thylakoid membranes in Triticum aestivum. It increases soluble sugars and free amino acid accumulation to protect plant cells from salinity- and drought-induced osmotic stress in Vigna unguiculata, Phaseolus vulgaris, and Pisum sativum. GB specifically increases its own content and improves the activity of antioxidant enzymes and metabolites, such as SOD, CAT, APX, proline and γ-amino butyric acid (GABA), reducing H2O2 and malondialdehyde (MDA) in Prunus persica, Lolium perenne, Glycine max, and O. sativa.

DNA microarrays performed to study the gene expression modification induced by the application of exogenous GB (100 mM) to Arabidopsis leaves and roots, revealed that the expression of genes encoding enzymes involved in ROS scavenging, as well as gene encoding functions related to membrane trafficking (RabA4c GTPase) and to extracellular ferric reduction (FRO2 and FRO4), was prevalently enhanced. This evidenced the involvement of stress-induced ROS signaling in GB action (Figure 2).

However, the GB applied to roots is usually taken up and accumulated in the cytosol and only a small amount is translocated to chloroplasts, while, when applied to leaves, it is translocated to meristematic tissues, in particular flower buds and shoot apices, and then translocated to actively growing and expanding tissues. also found that after the application of GB to mature leaves of H. vulgare under water stress, osmolytes were translocated to the young expanding tissues. Therefore, in plants even if GB is applied to old or mature tissues, it reallocates to young actively growing tissues, where its protective functions are mainly required.

Glycine Betaine Transport and Translocation

Although it is clear that GB endogenously accumulated or exogenously applied is reallocated in growing expanding tissues, knowledge on glycine transport and translocation remains fragmentary, and to date no specific transporters for GB have been reported in plants.

The first direct demonstration of a GB transport activity was obtained by through the heterologous expression of a tomato gene, homologous to Arabidopsis proline trasporter LeProT1, in the yeast mutant 22574d. The latter was unable to grow on citrulline, proline, or GABA as the sole nitrogen source; however, when complemented with the LeProT1 protein, it was able to transport proline and GABA with a low affinity and GB with a high affinity. also found that the Arabidopsis GABA transporter ProT2 was strongly inhibited by GB, with a high affinity to the osmolytes. ProTs could therefore be considered general carriers, which allow the transport of compatible solutes, including GB, with stress protecting functions. However, showed that in the betaine-accumulating mangrove, Avicennia marina under salinity LeProT1 mRNA accumulated only in pollen; while in other tissues there was an increase of mRNA for GB/proline A. marina transporters 1, 2, and 3 (AmT1, -2, and -3). AmT1 and -2 were able to complement salt-sensitive GB and a proline-deficient E. coli mutant. Moreover, the main accumulation of AmT1 under salinity was correlated to a major role for the transport of GB under osmotic stress. Subsequently, a gene homologous to AmT1, BvBet/ProT1, was isolated in Beta vulgaris by. A fusion protein GFP-BvBet/ProT1 was used to show the plasma membrane localization of the protein. In addition, both under control and salt-stress conditions, higher levels of CMO and BvBet/ProT1 mRNA were found in older leaves than in young leaves, further demonstrating that GB is mainly synthesized in older tissues and then translocated to young expanding ones. In situ hybridization experiments demonstrated that BvBet/ProT1 was localized in phloem and xylem parenchyma cells. A comparison between Bet/ProTs from non-accumulating (A. thaliana Col-O) and GB accumulating (B. vulgaris, Amaranthus tricolor, and Atriplex gmelinii) plants expressed in a yeast mutant deficient for uptake of proline and GB, showed that all the transporters had lower Km and therefore a higher affinity for GB than proline. The uptake of both osmolytes was pH-dependent, with GB uptake at a higher rate by BvBet/ProT1 when the pH decreased to 4.5 and underwent an inhibition by the proton uncoupler carbonylcyanide m-chlorophenylhydrazone (CCCP). The same transporters exhibited a higher affinity for choline uptake rather than GB, particularly at higher pH (6.5), and were less dependent on the inhibitor CCCP, suggesting that Pro/BetTs enacts a symport mechanism for GB/H+ and choline/H+ with a different mechanism of proton binding.

, which localized CMO exclusively in mature leaves of A. gmelinii, found that in the same plants the BetT gene was expressed in bladder and stalk cells, in meso-phyll cells of young leaf laminae and in vascular tissues. This finding is in agreement with the translocation experiments of 14C-labeled GB in H. vulgare, Brassica rapa ssp. oleifera, Glycine max, Pisum sativum, Lycopersicon esculentum, and T. aestivum which suggested a long-distance translocation of GB, together with photosynthetic assimilates, via phloem, and the phloem localization of BvBet/ProT1 found by. Moreover, demonstrated that when GB was applied to single mature leaves of tomato and accumulated in them, soon after, a large part of it was translocated to meristematic tissues, such as flower buds and shoot apices. In Arabidopsis and tomato GB-accumulating transgenic plants also translocated GB, via phloem, actively accumulating it in growing flower buds and shoot apices.

suggested that one possible explanation for why GB is firstly synthetized in expanded tissues and then transported to young expanding ones, is that for its synthesis it is necessary to reduce ferredoxin, which is primarily produced by mature leaves.

Glycine Betaine Role in Abiotic Stress Tolerance in Plants

Glycine betaine is one of the main compatible compounds present in Poaceae and Chenopodiaceae under salinity, and which is also involved in many other protective mechanisms against stress-related plant disorders. GB is an amphoteric metabolite highly soluble in water, and electrically neutral over a vast range of pH values. The cellular concentration of GB, proline, or both, contribute to the osmotic pressure as a whole in many halophyte plants. In glycophytes, GB is present at much lower levels than in halophytes. However, since it is compartmentalized solely to the cytosol and hyaloplasmic organelles, which account for about 20% of the volume of the cell or less, it is able to significantly contribute to the increase of osmotic pressure and can balance the vacuolar osmotic potential. GB does not only act as an osmolyte for osmotic adjustment, but, as a zwitterion, it can interact with both hydrophilic and hydrophobic domains of protein complexes and membranes: this contributes to stabilizing and maintaining the structural and functional integrity of these molecules, protecting them from the detrimental effects of highly reactive oxygen species (ROS). GB can reduce the salt-induced potassium efflux by regulating ion channels, and enhancing the enzymatic activity of plasma membrane H+-ATPase, increasing the phosphate uptake and regulating the phosphate homeostasis. Furthermore, it is able to preserve the thermodynamic stability of macromolecules, reversing protein misfolding and/or aggregation without compromising their native functional activities. When GB is present at high levels, together with proline, it is so efficient in protecting plants by oxidative stress that antioxidant metabolites and enzymes play a minor role in ROS protection under salinity.

Several beneficial effects of GB are summarized in Figure 2 and Table 2.

It has been proven that the synthesis of GB is ontogenetically controlled in several plant species (Table 3). However, in bread wheat and durum wheat, it is asynchronous compared to that of proline and independent of nitrogen nutrition. In fact, GB is synthetized and accumulated in young leaf tissues during prolonged stress, and, as a quaternary nitrogen compound, its synthesis is independent of nitrate nutrition. Whereas, proline is accumulated more rapidly at the onset of stress and primarily in older leaves dependent on high nitrate (Figure 3). The lack of nitrogen nutrition influence on GB synthesis and accumulation, implies that nitrogen reserves within the plant can be employed to fulfill the metabolic demands of osmolytes, resulting from salt stress. Therefore, GB and soluble sugars, like sucrose, but not proline, can play a major role in a plants adaptation to salinity under low nitrogen treatments; while proline is promptly synthetized, also in young tissues, under high nitrogen supply. However, GB and proline levels are highly correlated under salinity conditions, and their sum is equal in young expanding tissues of both high- and low-nitrogen grown plants. The presence of interchangeable levels of both compounds in young tissues, independent of nitrogen nutrition, would imply that resources are allocated in growing tissues in order to support and protect young growing tissues. The fact that the presence of one of these metabolites limits that of the other could also be due to the proposed GB-dependent inhibition of proline accumulation. However, the data of conflict with this assumption since they showed that the synthesis and accumulation of proline antedate those of GB, and that the use of an inhibitor of proline synthesis, hydroxyl-proline, decreases GB accumulation.

found that plants do not show a significant GB breakdown. Therefore, the low levels of GB present in older plant tissues depend on a dilution mechanism that occurs via GB translocation from fully expanded to young growing tissues, since the latter is more prone to stress, as reported in oilseed rape, turnip rape, bread, and durum wheat. also found that the contribution of GB to osmotic adjustment in younger tissues is much higher than that in older tissues, independently of nitrogen nutrition. Even the expression level of CMO and BADH proteins was preferentially induced in younger leaves in barley plants under salinity. Since GB is accumulated only during prolonged stresses, and it cannot be metabolized, even if easily and efficiently transported from older to younger plant tissues, it has been supposed that it can play a pivotal role in protecting against salt stress young leaf and root tissues. In view of this, ascribed the arrest of growth and differentiation of root tips of durum wheat under salinity to the delay in the synthesis of GB, which did not allow for the prompt contrast of the cytotoxic effect of the NaCl ions.

suggested that in young leaves of durum wheat plants, under high salinity the salt induced stomata closure restricts CO2 exchange and, consequently, reduces the RUBISCO CO2-fixation activity, while increasing the over-excitation of the photosynthetic apparatus and the production of ROS. In this condition, GB synthesis is induced to increase the protection of the photosynthetic apparatus.

Nevertheless, showed that the relevance of GBs synthesis in durum wheat, is almost completely inhibited by high light (HL) even in the presence of high concentrations of NaCl. proved that in these plants, in which GB was not accumulated, the fine metabolic regulation of few specific primary metabolites, such as GABA, amides, minor amino acids and hexoses, could play a key role in the plants response to simultaneous stresses. The positive effect of GABA can be ascribed to its proton consuming synthesis that allows for the control of pH, and its nature of zwitterion which permits its accumulation in cytosol, where it acts as an osmolyte and ROS scavenger, without toxic effects. However, a possible more relevant effect is that its synthesis, operated by the glutamate decarboxylase (GAD), releases CO2 that can be used for RUBISCO and the simultaneous dissipation of excess energy produced by photosynthesis under HL and salinity. This re-start of the Calvin cycle reduces the pressure on the photosynthetic electron chain and decreases ROS production and photodamage (, and references therein).

Conclusion

Metabolic engineering approaches and exogenous applications, aimed at increasing the synthesis and/or accumulation of GB in plants tissues, have been associated with the improvement in growth and survival of plants, ROS scavenging, osmoregulation of the cytosolic compartments, membrane stabilization, buffering of redox potential and induction of stress responsive genes that counteract the metabolism dysfunctions caused by stress. However, the efficacy of GB metabolism transformation for plant crops, cultured in field, has not been fully demonstrated. This might be because even if the GB concentration in transformed plants is significantly increased, it is still lower than that of a natural high accumulator species. Moreover, even if exogenous applications of GB that is targeted to the older damaged tissues has been tested, GB is promptly re-translocated to younger expanding tissues, where its protective functions are likely most required.

However, what is certainly clear is that in addition to this spatial discrepancy, that is the accumulation or re-allocation to young tissues after exogenous application, the synthesis of GB is also temporally delayed compared to other important osmolytes, such as proline. This most likely happens because GB cannot be metabolized. It is synthesized and accumulated only during extended stress, particularly in young tissues, as well as at low N nutrition. For this reason, it has been supposed that it plays a pivotal role in protecting young expanding tissues.

Author Contributions

All authors listed have made a substantial, direct and intellectual contribution to the work, and approved it for publication.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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    Saneoka H. Nagasaka C. Hahn D. T. Yang W. J. Premachandra G. S. Joly R. J. (1995 ). Salt tolerance of glycinebetaine-deficient and -containing maize lines. Plant Physiol. 107 631–638.

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