Construction of a high-density genetic map based on large-scale marker development in mango using specific-locus amplified fragment sequencing (SLAF-seq)

Genetic maps are particularly important and valuable tools for quantitative trait locus (QTL) mapping and marker assisted selection (MAS) of plant with desirable traits. In this study, 173 F1 plants from a cross between Mangifera indica L. ‘Jin-Hwang’ and M. indica L. ‘Irwin’ and their parent plants were subjected to high-throughput sequencing and specific-locus amplified fragment (SLAF) library construction. After preprocessing, 66.02 Gb of raw data containing 330.64 M reads were obtained. A total of 318,414 SLAFs were detected, of which 156,368 were polymorphic. Finally, 6,594 SLAFs were organized into a linkage map consisting of 20 linkage groups (LGs). The total length of the map was 3148.28 cM and the average distance between adjacent markers was 0.48 cM. This map could be considered, to our knowledge, the first high-density genetic map of mango, and might form the basis for fine QTL mapping and MAS of mango.

Abstract licence: CC BY

1. 13.

   SLAF-seq: An Efficient Method of Large-Scale De Novo SNP Discovery and Genotyping Using High-Throughput Sequencing · 2013

2. 15.

   Construction and Analysis of High-Density Linkage Map Using High-Throughput Sequencing Data · 2014

3. 28.

   Construction of a high-density genetic map for sesame based on large scale marker development by specific length amplified fragment (SLAF) sequencing · 2013

32 more references, whose wording the paper’s licence keeps private.

• The genome evolution and domestication of tropical fruit mango · 2020

A person counts once a day per paper. We keep no cookie and no address: a reader is a hash that changes every day. Any month under five reads shows no number.