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Cross-talk of nitric oxide and reactive oxygen species in plant programed cell death

Yiqin Wang, Gary J. Loake, Chengcai Chu

Frontiers in Plant Science · 2013 · Review

doi:10.3389/fpls.2013.00314

Abstract

In plants, programed cell death (PCD) is an important mechanism to regulate multiple aspects of growth and development, as well as to remove damaged or infected cells during responses to environmental stresses and pathogen attacks. Under biotic and abiotic stresses, plant cells exhibit a rapid synthesis of nitric oxide (NO) and a parallel accumulation of reactive oxygen species (ROS). Frequently, these responses trigger a PCD process leading to an intrinsic execution of plant cells. The accumulating evidence suggests that both NO and ROS play key roles in PCD. These redox active small molecules can trigger cell death either independently or synergistically. Here we summarize the recent progress on the cross-talk of NO and ROS signals in the hypersensitive response, leaf senescence, and other kinds of plant PCD caused by diverse cues.

Full text

From PubMed Central, U.S. National Library of Medicine, as current on Oct 27, 2026. Paragraph numbers are ours, so an answer can point at one.

NO, REACTIVE NITROGEN SPECIES, AND PROTEIN S-NITROSYLATION

Nitric oxide (NO) is a gaseous free radical which was first found to play a crucial role in plant and mediating defense reactions against bacterial pathogens. Increasing evidence suggests that NO, as a signal mediator, plays a key role in many physiological and developmental processes, such as germination, leaf expansion, lateral root development, flowering, stomatal closure, crosstalk with plant hormones, defenses against biotic and abiotic stresses. In plants, mitochondria and chloroplasts are organelles that are thought to contribute to NO generation in vivo. Although a long standing search for an NO synthase (NOS) in plants similar to NOS enzymes found in mammals has thus far been unsuccessful, suppression of NO signaling in the presence of NOS inhibitors has been reported by several groups, indicating the potential existence of a NOS-like enzyme in plants (; Figure 1).

As a free radical, NO could also react with various intracellular/extracellular targets and form a series of molecules, such as NO radicals (NO-), nitrosonium ions (NO+), peroxynitrite (ONOO-), S-nitrosothiols (SNOs), higher oxides of nitrogen (NOx) and dinitrosyl-iron complexes among others, collectively these NO derivatives are termed reactive nitrogen species (RNS; ). The functions of RNS, in plant cells are complex because they are implicated in many different physiological processes. S-nitrosylation, the covalent attachment of an NO moiety to a reactive cysteine thiol to form an SNO, has emerged as a prototypic redox-mediated modification in plants. For example, S-nitrosylation of methionine adenosyltransferase 1 (MAT1; ), the Arabidopsis type-II metacaspase AtMC9, PrxII E, a member of the peroxiredoxin family, non-expression of pathogenesis-related protein 1 (NPR1; ), Arabidopsis thaliana salicylic acid (SA) binding protein 3 (AtSABP3; ), TGACG motif binding factor 1 (TGA1) family, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase AtRBOHD, cytoskeletal proteins, auxin receptor-transport inhibitor response 1/auxin signaling F-box (TIR1/AFB; ), glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ) and also Arabidopsis histidine phosphotransfer protein (AHP1; ) have been reported. These data implies that protein S-nitrosylation is a key redox-based modification in plants and a pivotal mechanism to convey NO bioactivity. Peroxynitrite (ONOO-), formed from and NO, is also capable of reacting with many classes of biomolecules such as antioxidants and proteins, triggers defense responses in animals and plants. In Arabidopsis, ONOO- could induce hypersensitive response (HR) and defense-related gene expression. Very recently, protein tyrosine nitration, addition of an nitro group (NO2) to one of the two equivalent ortho carbons of the aromatic ring of Tyr residues and metal nitrosylation, was reported as a new important RNS-mediated post-translational modification. These findings not only deepen our understanding of NO signaling and function in plants, but also indicate the existence of RNS cross-talk with other signaling pathways, such as those orchestrated by auxin, cytokinin, SA, jasmonic acid (JA), ethylene (ET), and reactive oxygen species (ROS).

REACTIVE OXYGEN SPECIES

Reactive oxygen species including hydrogen peroxide (H2O2), superoxide anion, hydroxyl radicals (.OH) and singlet oxygen (1O2) have all been implicated in the control of biological processes in plants. Mitochondria as an “energy factory” are believed to be a major site of ROS production. Alternative oxidase (AOX) has an important influence on both ROS and RNS generation by the respiratory chain in mitochondria. Peroxisomes are subcellular organelles with an essentially oxidative type of metabolism and produce superoxide radicals as a consequence of their normal metabolism. Chloroplasts are also a major site of ROS generation in plants. The superoxide radicals and singlet oxygen (1O2) are produced in chloroplasts by photo-reduction of oxygen and energy transfer from triplet excited chlorophyll to oxygen, respectively (Figure 1).

Hydrogen peroxide, a ROS of major biological significance, can form as a result of the reaction of superoxide and also can be generated by specific enzymes. An oxidative burst, with rapid synthesis and its subsequent dismutation to H2O2 in the apoplast, is a common response to pathogens, elicitors, wounding, heat, ultra-violet light, and ozone. Besides its directly oxidative activity, it is now clear that H2O2 has a key signaling role in plants. H2O2 can induce gene expression and modulates signaling proteins, such as protein phosphatases (PP), protein kinases (PK), transcription factors and calcium channels that are located in the plasma membrane or elsewhere.

ROS AND NO SIGNALING IN THE HYPERSENSITIVE RESPONSE

A well-documented form of plant programed cell death (PCD) is the HR, characterized by the rapid cell death surrounding infection sites. The HR shows some similarity to the characteristics of animal apoptosis, such as membrane dysfunction, vacuolization of the cytoplasm, chromatin condensation, and endonucleolytic cleavage of DNA. Both NO and ROS have been implicated in controlling the HR process. One of the key determinants for the HR is the balance between intracellular NO and ROS levels. Following pathogen recognition, NO accumulation occurs concomitant with an oxidative burst, which consists of a biphasic production of apoplastic ROS at the site of attempted invasion. In this context, NO and H2O2 are thought to function in combination to promote HR cell death. For example, either of them could cause the release of cytochrome c from mitochondria, and affect the caspase-like signaling cascade, leading to the HR. Some key components of the defense signaling cascade that are known to be affected by ROS and NO activity include mitogen-activated protein kinases (MAPKs) and phosphatases (Figure 2). Thus, modulation of a central MAPK cascade may converge both H2O2 and NO signaling pathways activated in response to pathogen infection. In tomato cell suspensions, upon xylanase perception, cells activate a protein kinase pathway required for NO formation and S-nitrosylation-dependent mechanisms which are involved in downstream signaling, leading to production of polyamine and ROS production.

Interestingly, many proteins are targets of both NO and H2O2 (Figure 2). For example, GAPDH that plays a role in mediating ROS signaling in plants is a direct target of H2O2 and it is also a target of NO-mediated S-nitrosylation, which blunts its activity (Lindermayr et al., 2005). Also, MAT in mammals is inactivated by H2O2 through a reversible and covalent oxidation of a Cys residue. The same Cys residue is also a target for NO, which similarly causes enzyme inactivation (Hancock et al., 2005). Further, PrxII E not only reduces H2O2 and alkyl hydroperoxides, but also functions in detoxifying peroxynitrite. S-nitrosylation of PrxII E during the defense response regulates the antioxidant function of this key enzyme and might contribute to the HR (,; Figure 2). As a useful tool to elicit ROS-activated responses, ozone (O3) has been shown to induce HR-like cell death. During this process, NO accumulation preceded accumulation of ET, JA, SA, and leaf injury, implies that NO is an important signaling molecule in response to O3 exposure.

Contrary to its program cell death functions in the HR, NO can also scavenge H2O2 and protects plant cells from damage under certain circumstances. NO donors affect both wounding-induced H2O2 synthesis and wounding- or JA-induced expression of defense genes. In Arabidopsis, S-nitrosoglutathione reductase 1 (GSNOR1) is a key regulator that indirectly controls the global levels of protein S-nitrosylation (SNO). Loss-of-function mutations in GSNOR1 increased total cellular NO and SNO content and compromised both non-host and resistance (R) gene-mediated protection and also disabled basal defense responses (;; Figure 2). Further, the mutant atgsnor1–3 was also perturbed in thermotolerance and resistance to paraquat (1,1′-dimethyl-4,4′-bipyridinium dichloride), which induces the production of superoxide and H2O2 in wild type leaves. Consistent with these results, wild-type plants treated with an NO donor displayed resistance to paraquat. These studies showed that the Arabidopsis GSNOR1/HOT5/PAR-2 gene not only regulates SA signaling and thermotolerance by modulating the intracellular SNO level, but also acts downstream of superoxide to regulate cell death.

Interestingly, the increased levels of SNOs in atgsnor1–3 plants potentiated the HR even in the absence of the cell death agonist SA and apoplastic ROS synthesis. Surprisingly, NO S-nitrosylates the NADPH oxidase, AtRBOHD, at Cys890, diminishes its ability to synthesize ROS. This cysteine is also evolutionarily conserved and specifically S-nitrosylated in both human and fly NADPH oxidases, suggesting that this mechanism may govern immune responses in both plants and animals. Thus, NO may control ROS production through protein S-nitrosylation to further control the development of cell death processes. Collectively, these findings have provided significant insights into the understanding of the mechanisms underpinning ROS and RNS function in plants, revealing that the ROS/RNS pathway in plant PCD is highly complex and is mediated at least in part by crosstalk with several phytohormone signaling networks.

NO AND ROS CROSSTALK IN LEAF SENESCENCE

Leaf senescence, thought to be another form of plant PCD, is the final stage of leaf development, which is not only controlled by organ age but also triggered by adverse environmental factors. Additionally, phytohormones such as ET, SA, JA, auxin, ABA, and cytokinins all affect leaf senescence (Lim et al., 2007). In Arabidopsis, the level of H2O2 increases dramatically in leaf tissue during senescence. In addition to its role in oxidizing macromolecules such as proteins and lipids, H2O2 has also been proposed to function as a signal to induce the expression of genes involved in the senescence process. In agreement with its lower antioxidant capacity, senescent leaf tissue was found to contain elevated levels of ROS. In this context, a number of senescence-associated genes (SAGs) characterized from Arabidopsis could be induced by ozone (Miller et al., 1999) and the expression of many other SAGs were also induced by ROS, indicating that ROS might function as a signal to promote senescence. Interestingly, senescence-associated NAC genes (senNACs), key regulators of leaf senescence, were also found to be rapidly and strongly induced by H2O2 treatment in both leaves and roots. Thus, ROS has a dual role in leaf senescence: to promote the cell death process by directly oxidizing target macromolecules and to drive the expression of senescence-related genes.

Distinct from the positive role of ROS in senescence, NO can both provoke and impede this process, dependent upon its concentration and subcellular location. NO may alleviate the toxicity of ROS and has thus acted as a leaf senescence delaying factor in plants. The NO-deficient mutant nos1/noa1 showed early leaf senescence and similarly Arabidopsis expressing an NO degrading dioxygenase (NOD) displayed a senescence-like phenotype (; Figure 2). Furthermore, the level of NO is related with the senescence process and is thought to be an essential component involved in plant senescence signaling cascades. In Arabidopsis mutant dnd1, which lacks a plasma membrane-localized cation channel (CNGC2), early senescence-associated phenotypes (such as loss of chlorophyll, expression level of senescence associated genes, H2O2 generation, lipid peroxidation, tissue necrosis, and SA levels) were all elevated relative to wild type. Basal levels of NO in dnd1 leaves were lower than wild type, suggesting that the function of CNGC2 may impact downstream “basal” NO production in addition to its role linked to NO signaling. NO generation is therefore thought to act as a negative regulator during plant leaf senescence signaling. The protective effect of NO against ROS induced cell death can also be linked to the enhanced activity of antioxidant enzymes, as negative regulator of the chlorophyll catabolic pathway and as drivers for positively maintaining the stability of thylakoid membranes during leaf senescence.

On the other hand, NO can also promote the leaf senescence. Arabidopsis AtFer1, one of the best characterized plant ferritin isoforms to date, strongly accumulates upon treatment with excess iron, via an NO-mediated pathway. The AtFer1 isoform is functionally involved in events leading to the onset of age-dependent senescence in Arabidopsis and its iron-detoxification function during senescence is required when ROS accumulates. Recently identification of an NO accrual mutant noe1 (nitric oxide excess 1) in rice revealed that NOE1 encoded a rice catalase (CAT) OsCATC. Interestingly, noe1 plants exhibited an increase of H2O2 in their leaves, which consequently promoted NO production via activation of nitrate reductase. Removal of excess NO reduced cell death in both leaves and suspension cultures derived from noe1 plants, implicating that NO acts as an important endogenous mediator of H2O2-induced leaf cell death. Reduction of intracellular SNO levels, generated by over-expression of OsGSNOR alleviated leaf cell death in noe1 plants. Thus, S-nitrosylation was also involved in light-dependent leaf cell death in noe1. Collectively, these data suggest that both NO and SNOs are important mediators in the process of H2O2-induced leaf cell death in rice. OsGSNORin noe1 plants reduced SNO levels, consistent with a key role for this enzyme in SNO homeostasis. Moreover, the results show that no change in H2O2 content occurred in either GSNOR-overexpressing or GSNOR-RNAi transgenic linesin the context of noe1 background, suggesting that NO might function downstream of H2O2 in a light-driven leaf cell death in rice. It was found that NO treatment led to rapid cell death and induced H2O2 accumulation in maize leaves, and pharmacological studies also suggested that NO-induced cell death is in part mediated via H2O2, therefore H2O2 may be involved in NO-induced cell death in maize leaves. These discrepancies for the role of NO in cell death might be due to the differences in plant species, redox state, and growth conditions. Both NO and H2O2 could induce leaf cell death during which they could crosstalk with each other through different pathways.

NO AND ROS IN OTHER TYPES OF PLANT CELL DEATH

Some reports also describe the cross-talk of NO and ROS in other kinds of cell death in plants. Gibberellin (GA)-induced PCD in barley (Hordeum vulgare cv.Himalaya) aleurone layers is mediated by ROS and NO is a protective antioxidant. NO donors delay this PCD process, but do not inhibit metabolism in general, or the GA-induced synthesis and secretion of alpha-amylase. The amounts of CAT and superoxide dismutase (SOD) are greatly reduced in aleurone layers treated with GA. Treatment with GA in the presence of NO donors delays the loss of CAT and SOD. Thus, NO may be an endogenous modulator of PCD in barley aleurone cells. Furthermore, the exogenous application of NO rendered the plants more tolerant to arsenic (As)-induced oxidative damage by enhancing their antioxidant defense and glyoxalase system. Previous work has also shown that NO acts as a pivotal positive mediator in cadmium (Cd)-induced PCD in suspension cell cultures. NO strongly counteracts Cd-induced ROS mediated cytotoxicity in Brassica juncea by controlling antioxidant metabolism. Similarly, a role for NO as an antioxidant during heavy metal mediated toxicity has been highlighted recently by.

On the other hand, NO could also aid ROS-induced PCD. In pollen-pistil interactions, self-incompatibility (SI) induces relatively rapid and transient increases in ROS and NO. As ROS/NO scavengers alleviated both the formation of SI-induced actin punctate foci and also activation of a DEVDase/caspase-3-like activity. In tobacco BY-2 cells, sphinganine or dihydrosphingosine (d18:0, DHS) induce a calcium dependent PCD and trigger H2O2 production via the activation of NADPH oxidase(s). They also promote NO production, which is required for cell death induction. NO accumulated in Cd-induced PCD and promoted Cd-induced Arabidopsis PCD by promoting MPK6-mediated caspase-3-like activation. So the different roles of RNS in PCD and their crosstalk with ROS depend on the plant species, growth conditions and redox status.

CONCLUSION

In plants, RNS and ROS synthesis is a routine requirement for cells to undergo PCD, these small molecules can act either synergistically or independently. The accumulating data suggests significant cross-talk occurs between RNS and ROS (Figure 1), although the clear relationship of RNS and ROS in the process of cell death remains elusive. NO and ROS could regulate the synthesis each other. During HR, NO can affect ROS synthesis through S-nitrosylating NADPH oxidase AtRBOHD. On the other hand, in rice noe1 mutant, in the absence of OsNOE1/OsCATC function, the accumulation of H2O2 induces NO production through elevating nitrate reductase expression, which is further integral to H2O2 induced leaf cell death through S-nitrosylation of GAPDH and thioredoxin. Cross-talk of NO and H2O2 is a prominent feature in the activities of these small molecules. RNS and ROS also play important roles in modulating the activity of target proteins. A complete list of signaling pathways regulated by ROS or RNS still awaits identification, the data presented in this review are therefore far from offering a comprehensive picture of the function of NO and ROS during plant PCD. Thus, further work is needed to understand how these key molecules trigger the onset and development of plant cell death.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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    Rubbo H. Radi R. Trujillo M. Telleri R. Kalyanaraman B. Barnes S. (1994b ). Nitric oxide regulation of superoxide and peroxynitrite-dependent lipid peroxidation. Formation of novel nitrogen-containing oxidized lipid derivatives. J. Biol. Chem. 269 26066–26075

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    Saxena I. Shekhawat G. S. (2013 ). Nitric oxide (NO) in alleviation of heavy metal induced phytotoxicity and its role in protein nitration. Nitric Oxide 32 13–20

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    Tada Y. Spoel S. H. Pajerowska-Mukhtar K. Mou Z. Song J. Wang C. (2008 ). Plant immunity requires conformational changes [corrected] of NPR1 via S-nitrosylation and thioredoxins. Science 321 952–956

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    Tanou G. Filippou P. Belghazi M. Job D. Diamantidis G. Fotopoulos V. (2012 ). Oxidative and nitrosative-based signaling and associated post-translational modifications orchestrate the acclimation of citrus plants to salinity stress. Plant J. 72 585–599

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    Terrile M. C. Paris R. Calderon-Villalobos L. I. Iglesias M. J. Lamattina L. Estelle M. (2011 ). Nitric oxide influences auxin signaling through S-nitrosylation of the Arabidopsis transport inhibitor response1 auxin receptor. Plant J. 70 492–500

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    Tewari R. K. Prommer J. Watanabe M. (2013 ). Endogenous nitric oxide generation in protoplast chloroplasts. Plant Cell Rep. 32 31–44

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    Alternative Oxidase: A Mitochondrial Respiratory Pathway to Maintain Metabolic and Signaling Homeostasis during Abiotic and Biotic Stress in Plants · 2013
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    Verma K. Mehta S. K. Shekhawat G. S. (2013 ). Nitric oxide (NO) counteracts cadmium induced cytotoxic processes mediated by reactive oxygen species (ROS) in Brassica juncea: cross-talk between ROS, NO and antioxidant responses. Biometals 26 255–269

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    Wang Y. Chen C. Loake G. J. Chu C. (2010 ). Nitric oxide: promoter or suppressor of programmed cell death? Protein Cell 1 133–142

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    Wang Y. Lin A. Loake G. J. Chu C. (2013 ). H2O2-induced leaf cell death and the crosstalk of reactive nitric/oxygen species. J. Integr. Plant. Biol. 55 202–208

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    Wang Y. Q. Feechan A. Yun B. W. Shafiei R. Hofmann A. Taylor P. (2009 ). S-nitrosylation of AtSABP3 antagonizes the expression of plant immunity. J. Biol. Chem. 284 2131–2137

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    Wilkins K. A. Bancroft J. Bosch M. Ings J. Smirnoff N. Franklin-Tong V. E. (2011 ). ROS and NO mediate actin reorganization and programmed cell death in the self-incompatibility response of Papaver. Plant Physiol. 156 404–416

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    Wu X. Y. Kuai B. K. Jia J. Z. Jing H. C. (2012 ). Regulation of leaf senescence and crop genetic improvement. J. Integr. Plant. Biol. 54 936–952

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    Yadav S. David A. Baluska F. Bhatla S. C. (2013 ). Rapid auxin-induced nitric oxide accumulation and subsequent tyrosine nitration of proteins during adventitious root formation in sunflower hypocotyls. Plant Signal. Behav. 8 pii: e23196

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    Ye Y. Li Z. Xing D. (2013 ). Nitric oxide promotes MPK6-mediated caspase-3-like activation in cadmium-induced Arabidopsis thaliana programmed cell death. Plant Cell Environ. 36 1–15

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    Yemets A. I. Krasylenko Y. A. Lytvyn D. I. Sheremet Y. A. Blume Y. B. (2011 ). Nitric oxide signalling via cytoskeleton in plants. Plant Sci. 181 545–554

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    Yun B. W. Feechan A. Yin M. Saidi N. B. Le Bihan T. Yu M. (2011 ). S-nitrosylation of NADPH oxidase regulates cell death in plant immunity. Nature 478 264–268

    Not in our library10.1038/nature10427

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    Not in our library10.1104/pp.106.078857

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