The Impact of Oxidative Stress on Adipose Tissue Energy Balance

1White adipose tissue (WAT) is an endocrine organ that stores energy in the form of lipids and secretes hormones essential for insulin sensitivity and energy homeostasis. The fat cell interprets nutritional, hormonal, and sympathetic tone in the tissue microenvironment to store and liberate fuels until whole-body energy demands necessitate fatty acid liberation. Like other cells, mitochondria in adipocytes assimilate signals that reflect the energy status of the cell and produce the majority of ATP from macronutrients through cellular respiration and oxidative phosphorylation (OXPHOS).

2Obesity engenders nutrient stress that stifles mitochondrial capacity to sustain ATP levels in response to energy demands. Elevated mitochondrial substrate load consequently increases electron transport chain (ETC) activity and reactive oxygen species (ROS) production. Obese individuals exhibit higher levels of oxidative stress in WAT, including elevated ROS levels and decreased antioxidant activity coupled with alterations in adipokines required for insulin sensitivity. Moreover, oxidative stress associates with intra-abdominal obesity and insulin resistance. These results indicate the oxidizing environment in WAT of obese individuals likely impacts fat cell function and energy balance. Numerous questions remain, including how gradients of ROS inside the cell impact signaling cascades and gene regulation.

3Oxidative stress represents a disturbance in the equilibrium of ROS production and antioxidant defenses (Figure 1). At the molecular level, ROS mainly emerge from the mitochondrial ETC. Electron transfer through the ETC generates superoxide anions as byproducts, with complex I and III representing primary sources of ROS. Under certain conditions, complex II and other cellular ROS sources can contribute to the overall pool. Superoxide is the primary ROS species that reacts with Fe-containing proteins to generate H2O2. H2O2 accumulation in the cell contributes directly to the metabolic imbalance linking excessive nutrient stress and insulin resistance. However, ROS also encompass a diverse range of chemical entities, including nitric oxide, peroxynitrite, hypochlorous acid, singlet oxygen, and the hydroxyl radical. Consequently, the broad biological impacts of ROS derive from multiple cell and tissue microenvironments that divide physiological and pathological effects.

4The conditions that favor mitochondrial superoxide production include reduction of electron carrier pools associated with the mitochondrial respiratory chain (NADH, flavins, ubiquinone), a high proton motive force, and elevated oxygen consumption within the mitochondria. Overnutrition supplies excess electrons to the respiratory chain, while lack of physical activity and low ATP demand favors a high proton motive force with a low respiration rate, leading to mitochondrial superoxide formation and oxidative stress. By contrast, in mitochondria actively making ATP, superoxide production is low because the electron carriers are relatively oxidized, the proton motive force is small, and the respiration rate is high.

5Prolonged oxidative stress directly impacts metabolism, including the activity of enzymes involved in the TCA cycle and the ETC. The TCA cycle enzyme aconitase catalyzes the interconversion of citrate and isocitrate to regulate the availability of intermediates for lipid synthesis and ATP production. Citrate is the last common metabolite on the pathways for oxidation of acetyl-CoA and its export for fatty acid synthesis in the cytoplasm. Superoxide inhibits aconitase, leading to diversion of acetyl-CoA away from OXPHOS and toward fat storage. This feedback loop may be part of an antioxidant defense mechanism that adapts prolonged mitochondrial superoxide production. Acetyl-CoA diversion may slow delivery of electron carriers such as NADH to the respiratory chain, thereby decreasing ROS production.

6Oxidative stress also impacts pyruvate dehydrogenase kinase 2 (PDK2) inhibition of the pyruvate dehydrogenase complex (PDC) and fat metabolism. ROS oxidize critical cysteine residues, disabling PDK2, and supporting acetyl-CoA synthesis from glucose-derived pyruvate. Therefore, elevated mitochondrial superoxide and H2O2 couples PDC activity with aconitase interruption to divert citrate from the TCA cycle to the cytoplasm as triglycerides during overnutrition. These studies suggest persistent nutrient stress impairs the physiological behavior of crucial metabolic enzymes needed for balanced ATP generation and consumption.

7A variety of peroxidases, including catalase, glutathione peroxidases, and peroxiredoxins (Prdxs) that control the levels of H2O2 in the cell and protect against ROS-induced damage by catalyzing the reduction of H2O2 into water. Along these lines, overexpression of catalase or other methods that block H2O2 generation preserve insulin sensitivity in cell models and rodents fed high-fat diet.

8The mitochondrial antioxidant peroxiredoxin 3 (Prdx3) responds to oxidative stress and scavenges H2O2. Levels of Prdx3 are decreased in obese humans and mice, potentially contributing to oxidative stress intolerance. Whole-body deletion of Prdx3 in mice causes obesity and increased expression of lipogenic genes in adipocytes, while decreasing expression of lipolytic genes. As a result, hypertrophic adipocytes exclusively accumulate excess lipids and cannot enable appropriate energy balance control. In addition to altering the balance of lipogenesis and lipolysis, Prdx3-deficient adipocytes exhibited increased superoxide production, decreased mitochondrial potential, and altered adipokine expression, including decreased adiponectin.

9created “Fat ROS-augmented” (AKO) and “Fat ROS-eliminated” (aP2-dTg) mice to address the question of how ROS affect WAT function. AKO mice leverage adipocyte-specific ablation of glutamate-cysteine ligase (Gclc) to disable the rate-limiting step in glutathione synthesis and increase ROS generation. AKO mice fed high fat/high sucrose (HF/HS) diet for 6 weeks had smaller adipocytes and decreased expression of lipogenic genes, including Acly, Scd1, Fasn, Acaca, and Srebf1. Insulin sensitivity was also reduced. Conversely, mice expressing rat catalase and human SOD1 under the aP2 promoter had the opposite phenotype. These mice (aP2-dTg) showed reduced H2O2 in subcutaneous and gonadal WAT. Feeding a HF/HS diet yielded beneficial subcutaneous and gonadal WAT expansion mirrored by increased expression of lipogenic genes (Acly, Scd1, Fasn, and Acaca) and insulin sensitivity.

10While these data argue that increasing mitochondrial antioxidants protects against oxidative stress in WAT, genetic alteration of other mitochondrial antioxidants reveal different phenotypes. Manganese superoxide dismutase (MnSOD) is an important mitochondrial antioxidant that detoxifies superoxides. Adipocyte-specific knockout of MnSOD protected against diet-induced WAT expansion and weight gain. Mechanistically, MnSOD knockout in adipocytes triggered an adaptive stress response that activated mitochondrial biogenesis and enhanced mitochondrial fatty acid oxidation, thereby preventing diet-induced obesity and insulin resistance. Increased ROS levels correlated with Uncoupling Protein 1 (UCP1) activation in subcutaneous WAT and higher energy expenditure. These disparate features of mice that lack the Prdx3 and MnSOD genes coupled with therapeutic shortcomings of antioxidant therapies in human clinical trials suggest a more complex interaction of metabolism and redox balance in WAT.

11The homeostatic systems that regulate oxidative stress in the lean state are largely repressed in obesity due to the accumulation of oxidized biomolecules within WAT. Excessive ROS irreversibly damages DNA, lipids, and proteins with adverse effects on cellular functions. Increased oxidative stress can alter proteins and lipids through direct and indirect pathways that culminate in oxidation of side chains and lipid-protein adduction.

12Reactive oxygen species oxidation of lipids ultimately generates lipid aldehydes that modify DNA, proteins, RNA, and other lipid species. Increased markers of lipid peroxidation, including thiobarbituric acid reactive substances (TBARS) and 8-epi-prostaglandin-F2α (8-epi-PGF2α) are observed in individuals with higher BMI and waist circumference. Oxidized lipids and proteins preferentially accumulate in visceral depots compared to subcutaneous depots of obese mice and humans, suggesting ROS modifications correlate with conditions associated with type 2 diabetes, including central fat accrual.

13Highly reactive hydroxyl radicals (⋅OH) can be generated when excess H2O2 reacts with ferrous iron. Unlike H2O2, ⋅OH cannot undergo detoxification. Instead, ⋅OH removes electrons from neighboring lipids, proteins, and nucleic acids. Lipid aldehydes are highly electrophilic and prone to irreversible nucleophilic attack by the side chains of lysine (Lys), histidine (His), and cysteine (Cys) residues of proteins, resulting in a covalent lipid-protein adduct termed protein carbonylation. Furthermore, Lys, His, and Cys residues often cluster within active sites of enzymes or critical structural motifs, so their stable modification by lipids generally leads to inhibition or deactivation of protein function. However, recent work challenges the notion that ROS-driven modifications broadly degrade fat cell function. Brown adipose tissue (BAT) contains elevated levels of mitochondrial superoxide, mitochondrial H2O2, and oxidized lipids that correlate with acute activation of thermogenesis. Mitochondrial ROS in BAT can converge on UCP1 C253 inducing cysteine sulfenylation (-SOH). Interestingly, UCP1 C253A does not disable thermogenic responses in brown adipocytes but desensitizes the protein to adrenergic activation of uncoupled respiration. Further exploration of physiological ROS signaling outputs and modifications may show how redox status in adipocytes contributes to energy balance.

14Polyunsaturated fatty acids (PUFAs) are abundant in WAT and particularly sensitive to lipid peroxidation. One major consequence of lipid peroxidation is mitochondrial membrane damage. Also, peroxidation of PUFAs results in the release of diffusible reactive lipid aldehydes. Among the wide variety of reactive lipids formed through this mechanism, 4-hydroxy-non-enal (4-HNE) derived from oxidation of n6 fatty acids and 4-hydroxy-hexenal (4-HHE) from n3 fatty acid oxidation are the most widely studied in the context of adipose biology. The WAT of obese mice showed decreased metabolism of 4-HNE, while stress response proteins, including glutathione-S-transferase M1, glutathione peroxidase 1, and Prdx were carbonylated. Lipid peroxidation end products can also inhibit insulin signaling as 4-HNE de-stabilizes IRS adapter proteins and insulin receptor β.

15Lipid peroxidation products also damage the function of transcription factors that contain zinc-finger motifs, histones, and other nuclear proteins of visceral fat cells isolated from obese mice. The lipid peroxidation of transcriptional regulatory proteins presents a consolidated mechanism for retrograde ROS signaling from mitochondria to the nucleus. Although mitochondria are the most significant source of ROS, the discovery of lipid-protein adducts in the nucleus of adipocytes suggests either a different pool of ROS contributes to lipid peroxidation or a mechanism exists to sequester and shuttle reactive aldehydes to specific subcellular localizations. As with ROS, the timing of protein carbonylation may be important for beneficial or pathologic effects. Acute carbonylation of substrates after exercise are potentially beneficial, while chronic accumulation of carbonylated proteins in the muscle and WAT of obese and sedentary individuals may be pathological and contribute to comorbidities of obesity.

16Additionally, ROS seem to be important in the cellular aspects of adipocyte differentiation. Numerous studies demonstrate that mitochondrial biogenesis increases during adipocyte differentiation. Dramatic expansion of mitochondrial content enables higher metabolic rates to overcome the energetic demands of differentiation. Induction of differentiation correlates with superoxide generation from complex III, conversion of superoxide to H2O2, and activation of transcriptional machinery necessary for adipogenesis. Mechanistically, ROS production in differentiating cells coincides with increased C/EBPβ binding to DNA and accelerated mitotic clonal expansion. However, obesity-mediated ROS induction also restricts mitochondrial biogenesis and adipocyte differentiation. Higher accumulation of 4-HNE adducts occurs in cultured differentiating preadipocytes from insulin-resistant compared to insulin-sensitive individuals. In this manner, treatment of primary subcutaneous preadipocytes from obese individuals with pathological levels of 4-HNE decreased markers associated with insulin sensitivity and mature fat cells. Other studies demonstrate that treatment with antioxidants decreases differentiation and disrupts UCP1-dependent thermogenic responses. Divergent in vitro and in vivo findings illustrate existing challenges in defining the specifics of ROS signaling and its connectivity to metabolic diseases.

17Nutrient overload has been linked to the development of insulin resistance. In a pioneering study, healthy men fed ∼6000 kcal/day for 1 week exhibited WAT insulin resistance and oxidative stress in addition to protein oxidation and carbonylation. One carbonylated protein of importance was GLUT4, whose carbonylation likely impairs insulin-stimulated glucose uptake. Of note, systemic oxidative stress and insulin resistance did not coincide with inflammatory cytokines in plasma nor ER stress in WAT. These findings provide a causal link between oxidative stress and insulin resistance in humans.

18Mitochondrial metabolism is often altered in inherited diseases, such as inborn errors of metabolism (IEMs) that impinge upon ROS generation. Inhibition of OXPHOS increases ROS generation due to a backlog of electrons in the various complexes, resulting in electron leak, ROS generation, and production of H2O2. In IEMs affecting the ETC or other pathways of ATP generation, increased oxidative stress is often observed, while the exact mechanisms for increased ROS production are unknown. It is hypothesized that mutations affecting the formation of the protein complexes in the ETC or mutations that modify their assembly increase ROS generation by facilitating electron leak. Additionally, accumulation of toxic intermediates, often observed in IEMs, can increase the ROS generation by further decreasing OXPHOS activity, as in the case of medium-chain acyl-CoA dehydrogenase (MCAD) deficiency. MCAD deficiency reflects the accumulation of medium-chain fatty acid derivatives, including cis-4-decenoic acid, octanoate, and decanoate, with these metabolites altering levels of antioxidants and increasing markers of oxidative stress. Intriguingly, IEMs display metabolic reprograming with a switch to glycolysis for both ATP production and muted ROS generation. Specifically, in myoclonic epilepsy with ragged red fibers (MERRF), increased intracellular H2O2 levels correspond with increased AMPK phosphorylation and expression of GLUT1, hexokinase II, and lactate dehydrogenase. These results, as well as increased lactic acid production, all point to increased glycolysis. In multiple acyl-CoA dehydrogenase deficiency (MADD), mutations in ETFa, ETFb, or ETFDH, lead to decreased ATP production with an accumulation of organic acids, including glutaric acid as well as acyl-carnitines. A subset of these patients is riboflavin responsive (RR-MADD) with high dose riboflavin alleviating some symptoms. Similar to MERRF, many RR-MADD patients exhibit increased oxidative stress. This defect may be due to defective electron transfer and increased electron leak from the misfolded ETFDH protein and decreased binding of CoQ10. Treatment with CoQ10, but not riboflavin, decreased ROS levels. Analysis of mitochondrial function from RR-MADD fibroblasts showed increased mitochondrial fragmentation and reduced β-oxidation, while supplementation with the antioxidant CoQ10 decreased fragmentation and mitophagy. While obesity and IEMs are distinct disorders, both conditions impinge on energy balance in WAT. Even though these disorders have very different manifestations, oxidative stress plays an important role in both and may be a therapeutic target. For example, CoQ10 is often given as a broad-spectrum treatment to individuals with IEMs, and while its effectiveness is debated, the anti-inflammatory effects may be beneficial in reducing oxidative stress and the pathogenesis of the disease.

19Mitochondria represent control centers of many metabolic pathways. Interventions that enhance adipocyte mitochondrial function may also improve whole-body insulin sensitivity. Mitigation of mitochondrial ROS production and oxidative stress may be a possible therapeutic target in type 2 diabetes and IEMs because some mitochondrial-targeted antioxidants and other small molecule drugs improve metabolic profiles in mouse models and human studies.

20Thiazolidinediones (TZDs) are PPARγ agonists used for treating type 2 diabetes. TZDs, such as rosiglitazone and pioglitazone, enhance insulin sensitivity by improving adipokine profiles and reducing fasting blood glucose levels. TZDs also promote insulin sensitivity by directing fatty acids to subcutaneous fat, rather than visceral fat. Subcutaneous fat expandability, even in the context of obesity and type 2 diabetes, correlates with insulin sensitivity in rodents and humans. Numerous in vitro and in vivo studies demonstrate TZDs enhance mitochondrial biogenesis, content, function, and morphology. Rosiglitazone also induces cellular antioxidant enzymes responsible for the removal of ROS generated by increased mitochondrial activity in adipose tissue of diabetic rodents and humans. It is now well established that anti-diabetic PPARγ agonists also activate a BAT gene program in white adipocytes, converting them to “beige” cells that express UCP1. Taken together, TZDs impact WAT mitochondrial function in multiple ways that ultimately improve systemic fat metabolism and insulin sensitivity. Other therapeutic strategies include mitochondria-targeted scavengers and chemical uncouplers that dissipate energy as heat. However, these methods to enhance mitochondrial function display a narrow therapeutic range that limits safe use for obesity.

21Although the development of insulin resistance does not require impaired mitochondrial function, pathways promoting insulin resistance may impair mitochondrial function and further increase ROS production, resulting in a detrimental feedback loop. Aerobic exercise and caloric restriction disrupt this vicious loop, potentially by preventing accumulation of injured mitochondrial proteins with substantial improvement of insulin sensitivity. In insulin-resistant people, aerobic exercise stimulates both mitochondrial biogenesis and efficiency concurrent with an enhancement of insulin action. Ultimately, exercise engages pathways that reduce ROS coupled with insulin sensitivity and improved mitochondrial function in WAT.

22Obesity is the result of excessive expansion of WAT depots due to a chronic imbalance between energy intake and expenditure. Many studies demonstrate that oxidative stress in fat cells links obesity and its comorbidities. The fact that WAT remains the sole organ for storing surfeit lipid renders the macromolecules in adipocytes particularly vulnerable to carbonylation and other modifications driven by oxidative stress. Prolonged oxidative stress negatively influences endocrine and homeostatic performance of WAT, including disruption of hormone secretion, elevation of serum lipids, inadequate cellular antioxidant defenses, and impaired mitochondrial function (Figure 2). Metabolic challenges, such as persistent nutrient intake and sedentary behaviors that promote impaired glucose and lipid handling, also elevate mitochondrial ROS production to cause adipocyte dysfunction. Consequently, adipocytes cannot engage appropriate transcriptional and energetic responses to enable insulin sensitivity.

23The increasing prevalence of obesity suggests lifestyle intervention as the principal method to treat obesity is unlikely to succeed. Currently, all available anti-obesity medications act by limiting energy intake through appetite suppression or inhibition of intestinal lipid absorption. However, these medications are largely ineffective and often have adverse side effects. The central role of mitochondria in nutrient handling provides a logical entry point for improving metabolism in obesity. While approaches to understanding and intervening in oxidative damage evolve, exploration of mitochondria redox balance may enable development of dietary and small molecule therapies for obesity and its comorbidities.

24PM, AC, NC, and SH wrote the manuscript. SH secured funding.

25The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.