Rapid and Efficient Generation of Myelinating Human Oligodendrocytes in Organoids

Human stem cell derived brain organoids are increasingly gaining attention as an ideal model system for investigating neurological diseases, particularly those that involve myelination defects. However, current protocols for generating brain organoids with sufficiently mature oligodendrocytes that deposit myelin on endogenously produced neurons are lengthy and complicated. Taking advantage of a human pluripotent stem cell line that reports on SOX10 expression, we developed a protocol that involves a 42 day exposure of neuroectoderm-derived organoids to a cocktail of growth factors and small molecules that collectively foster oligodendrocyte specification and survival. Importantly, the resulting day 42 brain organoids contain both myelinating oligodendrocytes, cortical neuronal cells and astrocytes. These oligodendrocyte brain organoids therefore constitute a valuable and tractable platform for functional neurogenomics and drug screening for white matter diseases.

Abstract licence: CC BY

1. 3.

   Neuronal activity regulates remyelination via glutamate signalling to oligodendrocyte progenitors · 2015

2. 13.

   mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities · 2012

3. 17.
   Corrected

   Oligodendrocyte, Astrocyte, and Microglia Crosstalk in Myelin Development, Damage, and Repair · 2016

4. 23.

   Multiscale network modeling of oligodendrocytes reveals molecular components of myelin dysregulation in Alzheimer’s disease · 2017

5. 34.

   Oligodendrocytes in Development, Myelin Generation and Beyond · 2019

6. 37.

   Crosstalk Between Astrocytes and Microglia: An Overview · 2020

34 more references, whose wording the paper’s licence keeps private.

A person counts once a day per paper. We keep no cookie and no address: a reader is a hash that changes every day. Any month under five reads shows no number.