Myelin Dynamics Throughout Life: An Ever-Changing Landscape?

1The human brain undergoes extensive maturation throughout life to facilitate cognitive development. The myelination of axons throughout the nervous system is one such crucial maturation process. In the central nervous system (CNS), glial cells called oligodendrocytes extend many processes into their surrounding environment, which concentrically wrap membrane around axons to form myelin sheaths. Myelin sheaths enable the rapid saltatory conduction of action potentials, by localising voltage-gated Na+ channels to short gaps between adjacent sheaths (known as the nodes of Ranvier), and by acting as electrical insulators. Axons that are fully myelinated along their length conduct impulses many times faster than unmyelinated axons of the same cross sectional size. Therefore, myelinated neural circuits conduct information much faster than unmyelinated circuits. Humans are born with a virtually unmyelinated CNS, and the oligodendrocyte population expands dramatically following birth with widespread myelination in the first few years of childhood. Myelination continues through adolescence and into adulthood in a characteristic spatiotemporal manner, correlating with the emergence and maintenance of proper circuit function. For example, the maturation of white matter (the myelin-rich areas of the CNS) is concurrent with development of childhood cognitive processes, such as information processing speed. Additionally, myelin pathology/abnormalities are seen not only in the demyelinating disease Multiple Sclerosis, but also in several neurodegenerative diseases and neurodevelopmental disorders. However, myelination of individual axons is not an “all-or-nothing” phenomenon. Axons in the CNS exhibit extensive variation in myelin sheath number, sheath length, sheath thickness, and distribution along their length. Many different patterns of myelination exist; for example axons with sparsely myelinated regions have been described in juvenile and adult mouse cortex. Modification to any of these sheath parameters has predictable effects on the conduction speed of the underlying axon – therefore establishing a specific pattern of myelination along an axon may be particularly important to fine-tune circuit function. For instance, axons in the auditory brainstem of gerbils exhibit progressively shorter myelin sheaths along distal regions to ensure the precise timing of signal arrival to facilitate sound localisation. Subtle changes in the overall pattern of myelination along an axon (either via the addition of new myelin, or the remodelling of existing myelin) could profoundly change the timing of neural impulses in circuits. If myelin is adaptable then modifying such patterns of myelination may represent a powerful mechanism in regulating circuit function throughout life.

2Recent evidence suggests that myelin may be adaptable in response to circuit activity. Whole-brain diffusion tensor imaging can be used to measure broad changes in the myelin-rich white matter over time – such experiments in humans and rodents have shown that learning a new task correlates with white matter alterations in relevant brain regions. Cellular level analyses in animal models demonstrate that the production of new myelinating oligodendrocytes is required for efficient motor learning. It is currently hypothesised that neural circuit activity can trigger changes in myelin; an extensive body of research has demonstrated that neuronal activity can influence the proliferation of oligodendrocyte precursor cells (OPCs), the differentiation of oligodendrocytes, and the formation and growth of myelin sheaths. This research, including evidence for the molecular signals involved, has been extensively reviewed elsewhere. Neuronal activity could drive changes to myelin which could, in turn, change conduction speeds to fine-tune the timings underpinning circuit function.

3However, we still do not know whether or how the myelination of circuits is dynamically regulated throughout life. Rodent work indicates that new oligodendrocytes are generated throughout the CNS even in adulthood, and OPCs do reside within the human adult brain. Carbon-dating analysis of human tissue has identified adult-born oligodendrocytes within the cortex, although the same analyses indicated that the majority of oligodendrocytes in the corpus callosum originate in early childhood. However, the neuroimaging studies in humans that correlate white matter structural alterations with task learning suggest that new myelin can be formed throughout life. Such protracted myelination would in principle require lifelong oligodendrocyte production, given that individual myelinating oligodendrocytes have a restricted time window of just a few hours to initiate formation of new sheaths and sheath number per oligodendrocyte appears stable over time. One caveat noted by is that the “birth” date in the carbon-dating experiment (which identifies the time point of DNA replication during cell division), reflects that of the OPC, not necessarily the differentiated oligodendrocyte. This is important given evidence that OPCs can directly differentiate into oligodendrocytes without cell division, at least in rodents. OPCs in the corpus callosum could directly differentiate into oligodendrocytes many years after their terminal cell division; thus the time of differentiation of these new oligodendrocytes cannot be determined by carbon-dating, and so may have underestimated the rate of oligodendrocyte production in the adult human brain. We still have much to learn about the relative contributions of oligodendrocyte generation and myelin remodelling to CNS development throughout life.

4To fully understand the precise dynamics of oligodendrogenesis, myelin formation and myelin remodelling throughout various stages of life, longitudinal imaging at high-resolution represents the gold-standard approach. Here we provide an overview of recent in vivo imaging studies that are beginning to clarify the dynamics of myelination, which will also allow us to begin to understand how such dynamics might impact neural circuit function.

5To begin to definitively address how oligodendrocytes are generated and how myelin is made and dynamically remodelled in vivo, two recent studies utilised repeated two-photon imaging of the mouse somatosensory cortex over extended periods of time. imaged the cortex of transgenic reporter mice with fluorescently labelled oligodendroglial lineage cells from early adulthood, through middle and old age (approximately P720). They found that the oligodendrocyte population continues to expand and that cortical oligodendrocyte density nearly doubles between young adult and middle-aged stages (Figure 1A). This was accompanied by an over twofold increase in the number of cortical myelin sheaths. But how does oligodendrocyte number increase? In early post-natal development many oligodendrocytes are produced but only a subset survive and go on to myelinate axons. This appears to be similar in adulthood – by following individual cortical OPCs in the adult cortex for up to 50 days, revealed that the majority of newly differentiated oligodendrocytes undergo cell death, with only 22% surviving and committing to myelination (Figure 1B). It remains unknown what proportion of newly differentiated oligodendrocytes are generated following OPC division versus direct differentiation. However, once oligodendrocytes commit to myelination they remain stable, with no evidence of myelinating oligodendrocytes undergoing cell death during a 50-day imaging period.

6Similarly, used transgenic reporters of oligodendrocytes and the label-free spectral confocal reflectance (SCoRe) microscopy technique to image myelin along axons in the somatosensory cortex of juvenile, young adult, middle-aged, and old-aged mice (P950). They also found that the oligodendrocyte number continues to expand in adulthood up to P650, and that oligodendrocytes are stable in middle age for up to 80 days of imaging. They found that myelination of the cortex also peaks in middle age at P650, and that oligodendrocyte density significantly falls from its peak (at P650) through very old age (P950) (Figure 1A). This was reflected in a reduction of myelin coverage of layer I cortical axons between P650 and P950. Long-term oligodendrocyte survival may vary between different parts of the CNS. labelled myelinating oligodendrocytes at P60 in mice and then counted how many labelled cells survived until P605 in several CNS regions. They found that in the spinal cord and motor cortex, 60–70% of P60 labelled cells survived, whereas in the corpus callosum, over 90% of P60 labelled cells survived. The reduction in oligodendrocyte number and myelination in certain CNS regions with age raises intriguing questions concerning the role of myelin loss in age-associated cognitive decline. MRI analysis shows that white matter microstructure correlates with fluid intelligence, but also that this white matter microstructure deteriorates with increasing age. Subsequent age-associated myelin loss could lead to reduced cognitive function due to dysregulation of myelinated circuits.

7Could the generation of new oligodendrocytes (and subsequently new myelin) in the adult cortex be responsive to circuit activity? Previous research has shown that reducing sensory input by removing whiskers from mice leads to reduced oligodendrogenesis in the somatosensory cortex. To investigate this further, provided adult (P365) mice with sensory stimulation for 3 weeks by hanging beads in the animal cages to repeatedly stimulate their whiskers and thus the somatosensory cortex. By imaging the somatosensory cortex before and after the 3 weeks, they demonstrated that sensory stimulation increases oligodendrocyte number, potentially due to the increased survival of newly differentiated cells. provided further evidence that circuit activity may be important in regulating cell survival – they demonstrated that loss of AMPA receptor subunits 2, 3, and 4 in OPCs leads to reduced survival of oligodendrocytes. This suggests that de novo myelination could be modulated by cortical circuit activity throughout life, perhaps to fine-tune the function of those same circuits.

8Many questions remain to be addressed: what is the effect of oligodendrogenesis and new myelination on actual circuit function? Does neuronal activity enhance the long-term survival of myelinating oligodendrocytes? It is possible that the loss of oligodendrocytes in old age is due to age-associated reduction in neuronal activity, which might, in turn, affect overall oligodendrocyte survival. Alternatively, it may be that oligodendrocytes have a limited lifespan independent of neuronal activity (either intrinsically programmed or influenced by other extrinsic signals associated with aging). In either case, circuit stimulation could help alleviate age-associated myelin loss by either promoting survival of existing oligodendrocytes or stimulating the production of new oligodendrocytes. This in turn could have significant implications in the treatment and prevention of age-associated cognitive decline.

9Activity-mediated oligodendrogenesis is not restricted to the somatosensory cortex – young adult mice undergoing motor learning also show an increase in the number of newly differentiated oligodendrocytes in the motor cortex. What about other areas of the CNS? Many cortical axons project via the corpus callosum, and therefore, stimulation of cortical circuits could signal to both cortical and callosal OPCs. Two rodent studies have demonstrated that stimulation of cortical neurons induces oligodendrogenesis within the corpus callosum. optogenetically stimulated layer V projection neurons in the premotor cortex, finding a rise in OPC proliferation in both the premotor cortex and corpus callosum. This led to an increase in oligodendrocyte number and sheath thickness 4 weeks post-stimulation. More recently, used Designer Receptors Exclusively Activated by Designer Drugs to stimulate layer 2/3 somatosensory neurons, and also observed increased OPC proliferation, oligodendrogenesis, and thicker myelin sheaths in the corpus callosum in both juvenile and adult mice. They also demonstrated that new oligodendrocytes preferentially form myelin sheaths on the active axons. This indicates that activity-induced de novo myelination can, in principle, target active axons/circuits. It remains unknown how long-lasting changes to myelin in response to neuronal activity might be. The long-term survival of myelinating cells noted by,, and suggests that once an oligodendrocyte forms myelin sheaths it is likely to survive even if neuronal activity levels return back down to baseline. Whether the myelin sheaths themselves change once neuronal activity returns to normal levels requires more investigation of individual sheath dynamics, which is discussed below.

10Thus, it is possible that lifelong de novo myelination may occur in many CNS regions, where axons suitable for myelination have sufficient unmyelinated space. However, it remains unclear to what extent oligodendrogenesis continues in different areas of the adult human brain. Carbon-dating analysis suggests that most oligodendrocytes in the corpus callosum tract are generated in early childhood. Immunohistochemical analysis of human brain tissue using a novel marker for newly differentiated oligodendrocytes (BCAS1) shows new oligodendrocytes in the frontal cortex even beyond middle-age, but very few new oligodendrocytes in white matter after the third decade of life. This difference in oligodendrogenesis between species could be a result of scale. rodent data suggests that oligodendrocytes are generated in huge excess, with continuous pruning of almost 80% of cells. Given the energy cost of such a process, is this mechanism sustainable throughout life in an organ the size of the human brain? Perhaps in the human brain there is limited oligodendrocyte overproduction, because of a need for more protracted myelination of the larger CNS, or because signals such as neuronal activity stimulate OPCs to differentiate into oligodendrocytes as and when required.

11The remodelling of existing myelin sheaths could alter conduction properties without the need for generating new oligodendrocytes or myelin. Changing the lengths of existing myelin sheaths could change the myelin coverage along an axon and the distance between nodes of Ranvier (which would both impact conduction speeds). In addition, even very subtle myelin remodelling could alter the lengths of the nodes themselves. It has recently been shown that node length can vary extensively in the optic nerve and in the cortex, and that changing the node lengths along an axon can, in principle, also significantly alter conduction velocity. Whether changes to node of Ranvier are primarily driven by myelination or reorganisation of the axon itself remains to be determined.

12Both and performed longitudinal study of individual myelin sheaths in the mouse somatosensory cortex for several weeks to assess if sheath lengths are dynamically regulated. found that, in early adulthood (P90–120), although some sheaths exhibit extension or shrinkage, 81% of sheaths observed were stable. More sheaths may become stable in length with age; followed sheaths in older (P365) animals and saw that 99% of sheaths remained stable over 3 weeks (Figure 1B).

13Similar sheath length stability has also been described elsewhere; used larval zebrafish to investigate whether individual sheaths can change in length over time by performing time-course live imaging of fluorescently labelled myelin sheaths. They found that individual sheaths undergo rapid but variable growth in the first few days after formation, before stabilising their sheath lengths. Once stabilised, sheaths only continue to grow to accommodate the overall growth of the animal.

14Why do some sheaths in the cortex change in length, whilst others do not? This may reflect diversity in the demands of distinct neural circuits. Axonal diversity has been observed during initial myelination in the zebrafish spinal cord, where some axons use synaptic vesicle release to regulate myelin sheath number and length while others do not. This raises the intriguing hypothesis that only some axons are capable of regulating myelin via activity-related signals. found that their sensory stimulation paradigm did not increase the proportion of dynamic sheaths in the somatosensory cortex. However, more detailed analysis of axon subtype diversity coupled with longitudinal study of sheath length dynamics could confirm if sheath length remodelling is specific to certain circuits.

15Does sheath length stability reflect an inability of sheaths to remodel? Experiments in the zebrafish suggest that sheath length remodelling can be induced when the myelination profile of an axon is disrupted. ablated single oligodendrocytes and therefore sparsely removed sheaths along axons. They found that when a single myelin sheath is lost on a fully myelinated axon, the neighbouring sheaths could reinitiate rapid growth to cover the unmyelinated gap. In several cases a new myelin sheath would form in place of its predecessor and could even push back against the invading neighbour sheaths to restore the original pattern of myelination (Figure 2A). Therefore, sometimes a specific myelination pattern is preferentially maintained, even after myelin disruption. This may be to sustain the optimised conduction properties of the underlying axon. observed sparsely myelinated axons in the larval zebrafish, as previously identified in the rodent cortex. Interestingly, they found that upon ablation of single sheaths on such sparsely myelinated axons a new sheath formed in virtually the same place as the ablated sheath, even along an otherwise unmyelinated stretch of the axon (Figure 2B). Thus the myelination patterns along sparsely myelinated axons also appear to be stably maintained in zebrafish, as suggested by in rodents. The function of sparse myelination profiles remains unknown. Such patterns may allow for more dynamic fine-tuning of single axon function over time, although it is also possible that such unmyelinated gaps may facilitate gradual myelination to maintain consistent conduction times within circuits, as the animal grows and/or axon lengths change.

16Do stable myelin sheaths in mammals also have this capacity to remodel when the myelination pattern is disrupted? Further longitudinal studies coupled with demyelination are required to answer this question. It is possible that such remodelling is not induced by neuronal activity but is a compensatory mechanism for myelin loss. Age-associated oligodendrocyte loss could trigger remodelling of surviving sheaths to cover denuded portions of axon and therefore help maintain circuit function. Live imaging of myelin sheaths in old age could determine if this is the case.

17The live imaging studies discussed here have all assessed myelin sheath length dynamics, but not myelin sheath thickness. Can sheath thickness be dynamically regulated? Stimulating PI3K/AKT/mTOR signalling in oligodendrocytes of adult mice triggers additional myelin wrapping to increase sheath thickness. This may be modulated by circuit activity, as neuronal stimulation leads to increased sheath thickness in both juvenile and adult mice. This highlights the need to image all sheath parameters longitudinally to fully understand the dynamics of sheath remodelling. There is a need for live-imaging modalities to accurately measure sheath thickness along axons, as currently this requires cross-sectional measurement via electron microscopy, which limits analysis to a single time-point. Some label-free imaging techniques, such as third harmonic generation microscopy and spectral reflectometry, show promise for performing such measurements. Coupling these techniques with longitudinal studies of the rodent cortex could determine whether established myelin sheaths can adjust their thickness, or if neuronal activity simply pushes de novo myelination to produce thicker sheaths.

18It therefore seems that, although myelin sheaths are capable of remodelling when myelin is disrupted, most sheaths are generally stable in length. This stability is potentially due to the maintenance of early established myelination patterns optimised for circuit function.

19Recent mammalian imaging studies have focused on de novo myelination and sheath remodelling in cortical grey matter. Cortical circuits receive and send information via many regions, such as the spinal cord and corpus callosum, and so changes to myelin in several different CNS areas could alter signalling in a single circuit. The CNS is traditionally described by appearance after formaldehyde fixation, where “white matter” describes the heavily myelinated axonal tracts, while “grey matter” describes regions densely packed with neuronal cell bodies, dendrites, and synapses. However, this classification is overly simplistic; OPCs produce myelinating oligodendrocytes in both grey and white matter, and in fact there is emerging evidence of diversity in the oligodendroglial lineage and in patterns of myelination in both grey and white matter. Such diversity may reflect the unique requirements of myelin in distinct areas, and potentially on distinct circuits, of the CNS. Further longitudinal imaging studies are required to better understand the dynamics of de novo myelination and sheath remodelling in areas of the CNS beyond the cortex.

20While the optical transparency of the larval zebrafish lends itself to non-invasive live imaging, performing such experiments in the mammalian CNS is more invasive and technically challenging. and utilised two-photon microscopy with cranial imaging windows to image depths up to 400 μm into the cortex. Similar techniques could be used to image superficial myelinated tracts in the spinal cord over time but deeper CNS regions cannot be penetrated by two-photon microscopy alone. One alternative is to use two-photon microendoscopy, where a microendoscope probe with a gradient refractive index (GRIN) lens is inserted into the tissue to image cells deeper in the brain [previously used to image CA1 neurons of the hippocampus]. However, endoscope insertion may lead to inflammatory responses which could impact myelination. An alternative could be three-photon microscopy using the cranial imaging window method, which has also been previously used to image the hippocampus. Three-photon microscopy gives a significantly greater signal-to-background ratio than two-photon microscopy and can therefore be used to image deeper tissue structures.

21It is particularly important to consider not only different CNS regions, but different neurons within these regions. Previous research suggests that there are mechanistic differences in how distinct neuron subtypes regulate their myelination. Additionally, there may be diversity in local regulation of myelin. It is essential to remember that different parts of the CNS are not separate entities but are interconnected. Integrating mesoscale connectomics, which focuses on understanding the connections of different neuron subtypes across different regions, will be crucial to our understanding of how lifelong myelination dynamics vary between different circuits.

22What is the functional consequence of myelin regulation along distinct circuits? Thus far, the functional implications can only be inferred by correlations with behaviour. Ultimately, there is a need to couple measurement of myelin dynamics with direct assessment of circuit activity. This will require the recording of neuronal activity during longitudinal studies of myelination in order to directly connect de novo myelination or sheath remodelling observed to changes in circuit function with time. It will be important to measure the myelin dynamics and electrophysiological activity of individual neurons and axons to determine how changes in the various myelin sheath parameters actually affects the conduction properties at the single cell level, as well as assessing activity on a population level. Tools such as genetically encoded Ca2+ or voltage indicators allow relatively non-invasive recording of circuit activity, and can even be used to assess whole-brain circuit activity.

23The myelination of axons represents a powerful potential mechanism to regulate circuit function throughout life. Research has demonstrated that de novo myelination in the cortex (via the production of new oligodendrocytes) occurs even in adulthood, and that this can be enhanced by stimulating circuit activity. Once myelin has formed, it is stable with little turnover of oligodendrocytes and limited remodelling of the lengths of existing myelin sheaths. However, these stable structures may retain the capacity to remodel if myelin is disturbed. This has interesting implications concerning the plasticity of myelin in maintaining circuit function during injury, disease, and old age. Precisely how changes in myelination affect the function of the underlying circuit remains to be seen. Ultimately, a circuit-level approach, integrating analysis of myelin dynamics with direct measurement of circuit function, is required to fully appreciate how dynamic myelination influences overall nervous system function throughout life.

24All authors listed have made a substantial, direct and intellectual contribution to the work, and approved it for publication.

25The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.