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Pathogenicity Factors of Genomic Islands in Intestinal and Extraintestinal Escherichia coli

Mickaël Desvaux, Guillaume Dalmasso, Racha Beyrouthy and 3 more

Frontiers in Microbiology · 2020 · Review · CC BY

doi:10.3389/fmicb.2020.02065

Abstract

Escherichia coli is a versatile bacterial species that includes both harmless commensal strains and pathogenic strains found in the gastrointestinal tract in humans and warm-blooded animals. The growing amount of DNA sequence information generated in the era of “genomics” has helped to increase our understanding of the factors and mechanisms involved in the diversification of this bacterial species. The pathogenic side of E. coli that is afforded through horizontal transfers of genes encoding virulence factors enables this bacterium to become a highly diverse and adapted pathogen that is responsible for intestinal or extraintestinal diseases in humans and animals. Many of the accessory genes acquired by horizontal transfers form syntenic blocks and are recognised as genomic islands (GIs). These genomic regions contribute to the rapid evolution, diversification and adaptation of E. coli variants because they are frequently subject to rearrangements, excision and transfer, as well as to further acquisition of additional DNA. Here, we review a subgroup of GIs from E. coli termed pathogenicity islands (PAIs), a concept defined in the late 1980s by Jörg Hacker and colleagues in Werner Goebel’s group at the University of Würzburg, Würzburg, Germany. As with other GIs, the PAIs comprise large genomic regions that differ from the rest of the genome by their G+C content, by their typical insertion within transfer RNA genes, and by their harbouring of direct repeats (at their ends), integrase determinants, or other mobility loci. The hallmark of PAIs is their contribution to the emergence of virulent bacteria and to the development of intestinal and extraintestinal diseases. This review summarises the current knowledge on the structure and functional features of PAIs, on PAI-encoded E. coli pathogenicity factors and on the role of PAIs in host–pathogen interactions.

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From PubMed Central, U.S. National Library of Medicine, as current on Oct 24, 2026. Paragraph numbers are ours, so an answer can point at one.

Introduction

1Escherichia coli is a versatile bacterial species that has an extensive phylogenetic substructure comprising eight phylogroups (A, B1, B2, C, D, E, F, and G) that are roughly linked to the lifestyles of the different strains. E. coli strains colonize the gastrointestinal tract of human infants and coexist in good health with the host, with strains providing mutual benefits for decades. These commensal strains rarely cause diseases in healthy hosts, as they lack specialized virulence traits; they typically originate from phylogroup A. However, other E. coli strains acquire virulence attributes that confer them with the ability to adapt to new niches and cause intestinal and extraintestinal diseases. Consequently, E. coli strains can be classified into three groups: commensal/probiotic strains, intestinal-pathogenic strains and extraintestinal-pathogenic strains.

2Intestinal-pathogenic E. coli (InPEC), which are rarely encountered in the fecal flora of healthy hosts, cause gastroenteritis or colitis. Six well-described pathotypes of diarrheagenic E. coli (DEC) have been identified: enteropathogenic E. coli (EPEC), enterohemorrhagic E. coli (EHEC), enterotoxigenic E. coli (ETEC), enteroaggregative E. coli (EAEC), enteroinvasive E. coli (EIEC) and diffusely adherent E. coli (DAEC). The DEC pathotypes can share certain virulence features; however, each pathotype possesses a specific combination of virulence traits that results in a distinctive pathogenic mechanism. The EPEC strains are not specifically classified as a phylogroup, although some studies preferentially associate them with phylogroup B1. The EHEC are distributed preferentially between phylogroups A and B1. However, the EHEC of serotype O157: H7 belongs to phylogroup E.

3Extraintestinal E. coli (ExPEC) are facultative pathogens responsible for 80% of urinary tract infections (UTIs) in outpatients. Infection by ExPEC leads to a large portion of nosocomial UTIs (50%) and is the leading cause of abscesses, accounting for 30% of meningitis in neonates. Bacteremia and septic shock can accompany infections at any site. ExPEC typically belong to the phylogroup B2 – an E. coli genetic background that accumulates virulence factors – and occasionally to phylogroups D, F or G. Group B2 has the greatest diversity among all E. coli phylogroups, suggesting that it has subspecies status and includes subgroups correlated with a flexible gene pool. This flexible gene pool comprises genes for various combinations of virulence factors, such as adhesins, iron-acquisition systems, host defense-avoidance mechanisms and toxins. The link between the evolutionary lineages of E. coli, certain extraintestinal virulence genes and infection sites has led to the concepts of uropathogenic E. coli (UPEC), sepsis-associated E. coli (SEPEC), neonatal meningitis E. coli (NMEC) and avian pathogenic E. coli (APEC; which cause extraintestinal infections in poultry). Finally, a non-diarrheagenic InEC pathotype, called adherent-invasive E. coli (AIEC), has been noted for its association with inflammatory bowel diseases such as Crohn’s disease. AIEC is considered a pathobiont bacterium rather than a bacterium responsible for acute infection, and it frequently belongs to the phylogroup B2, like ExPEC, and shares with ExPEC the most virulence traits.

4Whole-genome E. coli phylogeny suggests an evolution of ExPEC from commensal E. coli strains that were originally devoid of virulence factors but became pathogenic because of horizontal gene transfers involving transduction, transformation, and conjugation events. Phages, plasmids and large parts of the genome, designated as genomic islands (GIs), were transferred from one bacterium to another. These types of GIs carrying virulence-associated genes were identified in UPEC in the early 1980s by, and were designated as pathogenicity islands (PAIs). Since then, PAIs have been described from SEPEC, MNEC and diarrheagenic isolates and in other species, such as Salmonella enterica. The species Escherichia coli and Salmonella enterica share 70% of their genome and have recently diverged. The divergence of both species has been, in large part, due the acquisition of specific PAIs and has resulted in very different lifestyles. This review summarizes the current knowledge on the structure and functional features of PAIs, on E. coli PAI-encoded pathogenicity factors and on the role of PAIs in host-pathogen interactions.

Structural Features of Pathogenicity Islands

5Pathogenicity islands are a group of large (>10 kb) integrative elements that encode one or more virulence genes that are absent from the genomes of non-pathogenic representative bacteria of the same species or of closely related species (; Hacker and;; ). In contrast to other integrative elements, such as bacteriophages, plasmids or integrative and conjugative elements (ICEs), PAIs are non-replicative and lack the ability to self-mobilize.

6Comparison of the genomic region of PAIs and the remaining parts of the host genome shows that PAIs have their own genomic characteristics, which is strong evidence of their foreign origin and horizontal acquisition. The G + C contents (i.e., the percentage of guanine and cytosine bases), the frequency of dinucleotides or high-order oligonucleotides and the codon usage in PAIs often differ from those of the host organisms. For instance, the G + C content of the 536 UPEC core genome is 50%, while the G + C content is 41% in the PAIs I536, II536, IV536, and V536. In the EPEC genome, the G + C content of the LEE (locus of enterocyte effacement) PAI is only 39%, while the G + C content of the E. coli core genome is ∼50%. However, the donor and recipient organisms may possibly have a similar G + C sequence composition, thereby complicating the extraction of PAIs from the core genome. Even for donor and recipient organisms with different sequence compositions, the PAI region can be “ameliorated” throughout evolution, making the sequence composition or codon usage of the PAI region similar to that of the core genome. The divergences in GC content can therefore reflect a recent acquisition and/or an evolutionary mechanism that maintains a divergence in GC content. This divergence can have a functional role, since curved and AT-rich PAIs are preferential targets of the global gene silencer H-NS. The H-NS-mediated silencing prevents the uncontrolled transcription of genes within PAIs to ensure that bacterial fitness is maintained, and it may also have evolutionary consequences by influencing the acquisition and maintenance of foreign DNA.

7Nonetheless, most GIs contain a recombination module (Figure 1A) that is also observed in other integrative elements, such as phages, integrons, conjugative transposons and ICEs. The module consists of three parts: (i) an integrase of the tyrosine recombinase family; (ii) two flanking attachment sites forming direct repeats resembling the attR and attL sites of prophages; and, in some cases, and (iii) a recombination directionality factor (RDF). This recombination module is characteristic of the integrative elements that insert a circular intermediate into the host genome. However, island-encoded integrases form a separate clade within the tyrosine recombinase family. Therefore, PAIs are a distinct class of integrative elements and are not degenerate remnants of other mobile elements.

8Most island-related integrases are inserted adjacent to tRNA or transfer-messenger RNA (tmRNA) loci (Figure 1A). However, the number of specific tRNA genes used is limited. Within the E. coli genome, ∼87 tRNA genes have been annotated, and integrative genetic elements use only a handful of these sites. Fifteen tDNAs are hotspots of integration, with asnT, aspV, leuX, metV, pheV, and thrW as the most frequently targeted genes. The tDNA pattern targeted by insertions may differ in B2 and A/B1 E. coli strains, potentially influencing their ability to acquire and lose PAIs.

9Pathogenicity islands often include mobile elements (or fragments thereof), such as bacteriophages, plasmids and insertion sequences. These mobile elements play an important role in recombination, resulting in genetic rearrangements, insertions, deletions and, therefore, variation of PAIs. The elements contribute to the formation of mosaic-like structures, another hallmark of PAIs that promotes the accretion of traits into islands.

10A number of E. coli PAIs have the PAI features mentioned above; however, some lack one, two or even more features, making the detection of PAIs from sequenced genomes a challenge. The detection methods usually use the following indicative features of the horizontal origin of GIs and PAIs: (i) biased sequence composition, (ii) gene or motif content (i.e., tRNA/tmRNA, direct repeats, integrases, mobility-related genes, high prevalence of hypothetical proteins and virulence/metabolic/antibiotic resistance genes to subclassify the GIs), and (iii) sporadic phylogenetic distribution assessed by the identification of regions only present in a subset of genomes and/or containing genes usually found in PAIs, such as virulence genes, transposase, integrases or genes coding unknown functions. Composite detection methods, such as IslandViewer and GIHunter, are the most sensitive methods. The data with the highest accuracy are provided by tools such as Islander, which can detect tRNA sequence direct repeats. Using these in silico approaches, the databases of predicted or curated GIs have been developed from publicly available genome sequences and provide a large sampling of structural variations and gene content in PAIs.

Instability and Motiliy of Pathogenicity Islands

11Pathogenicity islands in ExPEC and InPEC can undergo deletion at frequencies ranging from 5 × 10–3 to 1 × 10–6. In many cases, excision and integration seem to be mediated by PAI-encoded integrases. These harbor a highly conserved C-terminal domain involved in recombination and a more divergent N-terminal domain that specifically recognizes the integration site attB (Figure 1A). During the acquisition, the PAI-specific attachment site attP is frequently integrated adjacent to the 3’ end of tDNAs, as observed for phage integration. The recombination results in the direct duplication of the attachment site attB, which forms 16 to 130 bp direct repeats (attL and attR) that flank the PAIs. If multiple isoacceptor tDNAs exist, chromosomal insertion may occur at all available loci, as observed for the high-pathogenicity island (HPI), LEE PAI or PAI IIJ96. Integrase also excises PAIs from the genome as circular non-replicative intermediates (Figure 1A) using a site-specific recombination process. In UPEC strain 536, excision of PAIs I536, II536 and III536 depends on their own integrases. However, PAI V536 undergoes excision even in the absence of its integrase, which can be substituted by the integrase of PAI V536, suggesting crosstalk between PAIs. Enzymes called excisionases or recombination directionality factors (RDFs) can assist integrases. A bioinformatic analysis showed that each PAI in UPEC 536 contained its own cognate putative RDF. RDFs act as positive or negative integrase transcriptional regulators and offer stability to their integrase protein partners at the excision site. The mobility of PAI can also be independent of the recombination module and involve homologous DNA recombination.

12Horizontal transfer (HT) is another aspect of PAI mobility (Figure 1B). Because most islands do not contain an origin of replication and are not able to self-mobilize, HT of excised PAIs has been hypothesized to occur with the help of bacteriophages, ICEs or conjugative plasmids. The presence of phage-related sequences on most PAIs suggests that phages have a key role in HT. Alternatively, PAIs can be transferred by conjugation in the presence of an attB-presenting helper replicon and accessory transfer genes. An alternative mechanism, independent of the att site, is homologous DNA recombination, which involves sequences shared by plasmids, a PAI or its environment, and the recipient genome. The stabilization of beneficial genetic information localized on mobile genetic elements can then be achieved by the selective loss of transfer or mobilization functions encoded by these elements.

Physiopathology of ExPEC and AIEC Infections

13The non-diarrheagenic E. coli are opportunistic pathogens. ExPEC take advantage of host behavior and susceptibility by employing virulence factors (Figure 2) to colonize the digestive tract and then move on to the bladder, where they cause cystitis. The cystitis infection can ascend through the ureters to the kidneys, eventually causing pyelonephritis, and potentially reaching the blood compartment to cause sepsis. ExPEC use cell-surface adhesins to adhere to the host’s epithelial cells. Adhesin-receptor interactions, invasion factors and bacterial engulfment-enhancing toxins stimulate bacterial internalization into apical uroepithelium cells, leading to invading bacteria that are endocytosed into membrane vesicles. The ExPEC then escape from the endocytic vesicles, proliferate inside the cellular cytoplasm, and form intracellular bacterial communities (IBCs).

14Intracellular bacterial communities constitute biofilm-like quiescent reservoirs that preserves ExPEC viability and can lead to recurrent UTIs. Cytosolic E. coli also induce cell lysis and exfoliation. The innate immune response initiated by infected uroepithelium cells and exfoliation clears many bacteria from the urinary tract with the flow of urine, but this also leaves the underlying layers of immature bladder epithelial cells exposed and more susceptible to infection. In a small proportion of UTIs, ExPEC will decrease the expression of bladder-targeting adhesins and enhance the expression of both kidney-targeting adhesins and bacterial motility. Adhesion and mobility allow ExPEC to ascend through the ureters to the kidneys, where they cause acute pyelonephritis.

15Asymptomatic bacteriuria (ABU) occurs in up to 6% of healthy individuals and in 20% of elderly individuals. ABU-associated E. coli persist for months or years without evoking a destructive mucosal host response. E. coli strains lacking virulence genes and a defect in TLR4 signaling lead to the asymptomatic carrier state.

16Bacterial destabilization of the renal epithelium and subsequent inflammation result in renal scarring, which acts as a conduit to the bloodstream. One study showed that 91% of E. coli strains isolated from the blood and urine of the same hospitalized patients were closely related, suggesting that UPEC is the main origin of septicemias and SEPEC. The strains originating from UTIs can also be found in the guts of the patients. Accordingly, overgrowing intestinal ExPEC strains can also translocate through the gut epithelium and survive in mesenteric lymph nodes to enter the bloodstream. Throughout their progression, ExPEC use numerous capture systems to find the necessary elements, particularly iron, to aid their growth. In the bloodstream, SEPEC protect themselves from the immune system by surface structures and effectors which allow them to escape the bactericidal activity of complement and phagocytosis. This resistance is a factor in the induction of a high degree of bacteremia.

17Several in vivo studies on NMEC have pointed out a relationship between the magnitude of bacteremia and the development of meningitis. E. coli binding and the invasion of human brain microvascular endothelial cells (HBMECs) mediated by E. coli pathogenicity factors is also a prerequisite. The vacuoles containing NMEC that result from HBMEC invasion evolve as endosomes without fusion with lysosomes, thereby allowing E. coli to cross the blood-brain barrier alive.

18Avian pathogenic E. coli strains cause avian colibacillosis, a poultry extraintestinal infection. Extensive genetic similarity has been documented between APEC and ExPEC strains. APEC can cause diseases in mammalian infection models, and these diseases mimic human ExPEC infections. Conversely, human sources of ExPEC can cause diseases in avian species. Human ExPEC and APEC therefore share similar pathogenic potential, and some human-associated ExPEC may have evolved from APEC, and vice versa.

19Adherent-invasive E. coli strains have an increased prevalence in inflammatory bowel disease (IBD). AIEC strains harbor genetic similarity with ExPEC in terms of phylogenetic origin and pathogenicity genotype. However, only 6.3% of ExPEC strains exhibit AIEC phenotypic features, suggesting that the AIEC pathotype is disease specific and contributes to the pathophysiology of chronic IBD. AIEC bacteria interact with the intestinal mucosa and can (i) cross the mucus layer and resist antimicrobial peptides, (ii) adhere to the surface of the mucosa and increase intestinal permeability, and (iii) penetrate the lamina propria, persist in macrophage vacuoles and chronically stimulate the immune system. Apart from having a high genotypic variability, AIEC belong mainly to phylogroup B2 and resemble ExPEC in terms of virulence factor content. However, AIEC could have selected an original regulatory system and/or pathoadaptive mutations to enable better adaptation to intestinal conditions.

PAIs in ExPEC and AIEC

20Experimental and epidemiological investigations of ExPEC pathogenicity factors (PFs) show that no PF is limited to, and no single clone is required for, any particular extraintestinal infection. Statistical analyses of PF content and the resulting lethality in an animal model suggested that the virulence of ExPEC is more likely linked to the number of PFs than to the genetic background. In addition, the success of pathogens at various host sites can be due to multiple combinations of PFs, and virulence potential probably exists as a continuum that depends on the number and type of PFs and the inoculum size. The prevalence of PFs in ExPEC infections is reported in Table 1.

21Most ExPEC PFs are encoded by PAIs (Table 2), and the encoded functions are involved in critical steps of the infectious process. The cooperative effect of PAIs has been experimentally confirmed with the prototype strains 536, CFT073 and RS218 by monitoring UTI, septicemia and meningitis mouse models using single-PAI and/or multiple-PAI deletion mutants. These studies showed an additive contribution of PAIs to extraintestinal virulence, but redundancies (i.e., iron uptake) were also evident. The involvement of PAIs in virulence also depends on the genetic background and on the infection model used to test them, suggesting a complex network of pathogenetic possibilities resulting from multiple and independent acquisitions of functions without an unequivocal goal. Given the functional redundancies in PAIs, and to provide an overview of the pathogenicity functions encoded by ExPEC PAIs, their PF content is presented here by PF type and the main structural features of corresponding PAIs is reported in Table 2 and Figure 3 for reference strains.

Adhesins Encoded or Regulated by PAIs

22The capacity of E. coli to adhere to host cells plays a fundamental role in the colonization of any site. Adhesion is mediated by proteins called adhesins, which specifically recognize receptors and are often contain antigenic sugars that are present on the surface of host cells. Adhesins are exposed directly to the surface of bacteria or are carried by filamentous structures called “fimbriae” or “pili” (Table 1). Most adhesins are encoded by PAIs, except for conserved fimbriae, such as type 1 fimbriae, curly fibers, outer membrane protein A (OmpA), chitin-binding domain of chiA and new lipoprotein I (NlpI) that are involved in the adhesion of AIEC and NMEC to enterocytes and HBMECs.

23Type 1 fimbriae are present in more than 80% of E. coli strains and other Enterobacteriales. However, they are major players in ExPEC virulence, and their expression is regulated by PAIs. The type 1 fimbria adhesin FimH binds specifically to α-D-mannose residues attached to membrane glycoproteins exposed at the surface of bladder cells, enterocytes and the endothelial cells of brain capillaries. The role of type 1 pili in cystitis has been demonstrated by mutagenesis and in animal models. Their adhesin FimH binds to glycosylated uroplakin and α1β3 integrins covering the apical surface of uroepithelium cells, thereby promoting the invasion of host cells and the development of IBCs. Notably, in some AIEC, UPEC, MNEC and APEC strains, the protein sequence of FimH adhesin has polymorphisms that confer a greater ability to interact with D-mannose.

24The expression of type 1 pili depends on a promoter encoded by the phase-variation invertible element fimS. The orientation of fimS can be reversed by the recombinases FimB and FimE encoded within the operon Fim, and by recombinases FimX, IpuA and IpbA encoded by PAIs. IpbA is ubiquitous, as it is found approximately half the time in both commensal fecal and uropathogenic isolates of E. coli. FimX-encoded PAI is observed in more than 80% of UPEC and is more prevalent in the UPEC of a lower urinary tract origin (87.5%) than of upper urinary tract origin (74%) or of commensal isolates (36%). IpuA-encoding PAIs also tend to occur more frequently among UPEC strains than in commensal fecal isolates. This observation is consistent with the hypothesis that FimW and IpuA recombinases play a role in the regulation of E. coli uropathogenesis. FimX, IpuA and IpbA promote inversion of fimS from OFF to ON and probably promote bladder colonization, while FimE inverts the promoter from ON to OFF and could therefore promote the release of bacteria from the bladder epithelium and the infection of the higher tract. The latter might be enhanced by a coordinated upregulation of both bacterial mobility and other adhesins, such as P fimbriae.

25FimX also regulates the predicted LuxR-like response regulator HyxR, which is encoded from the same PAI and is a negative regulator of the nitrosative stress response and intracellular macrophage survival. IpuA and the associated recombinase IpuB, which lacks activity on fimS, regulate the orientation of a phase-variable invertible element ipuS, which is located in the same PAI, proximal to ipuA and ipuB. The orientation of ipuS drives the transcription of a two-gene operon containing ipuR (a predicted LuxR-type regulator) and upaE (an autotransporter involved in biofilm formation and extracellular matrix adhesion). Consistent with this phenotype, the ipuS orientation ON results in (i) defective swimming motility, (ii) an increase in adhesion to human kidney epithelial cells, and (iii) promotion of kidney colonization in experimental UTI mouse models. Overall, therefore, PAIs not only encode PFs, but they are also involved in virulence regulation networks.

26P fimbriae are encoded by the pap operon (pyelonephritis associated pili), which is associated with different PAIs. The non-structural gene papX of the pap operon is another example of the PAI gene that is involved in virulence regulation. The protein PapX binds directly to the flhDC promoter, thereby inhibiting the transcription of the master regulator of flagellar biosynthesis, motility and chemotaxis. The fimbrial-tip adhesin of the P fimbria PapG binds to glycosphingolipids containing the digalactoside Gal(α1-4β) Gal moieties found in the renal epithelium, P blood-group antigen and gut epithelium. Three major alleles of the papG locus (GI to III) have been described, with alleles GII and GIII being the most frequently encountered in human disease. Allele GII recognizes globotriosylceramide (GbO3) and globotetraosylceramide (GbO4),which are particularly abundant in the kidneys, and it is preferentially found in strains isolated from pyelonephritis because allele GIII recognizes globopentaosylceramide (GbO5) and is mainly found in strains isolated from cystitis. GbO5 is found in the urothelium of animals but is not present in humans, except in certain individuals expressing an analog (GloboA) and who are particularly susceptible to lower UTIs with PapGIII strains.

27PAI-CFT073-aspV harbors the tos operon, which encodes the repeat-in-toxin (RTX) family member TosA. The RTX protein family members can be involved in a range of functions, including pore and biofilm formation and adhesion to host cells. The presence of tosA promotes bladder and kidney colonization in a UTI mouse model and is a marker of UPEC. Localized to the bacterial surface, TosA mediates adhesion to host cells derived from the upper urinary tract, increases bacterial survival in disseminated infections and enhances lethality during sepsis. The TosCBD proteins mediate the production and export of TosA, while TosE and TosF suppress motility by an unknown regulatory function. TosR is a member of the PapB family of transcriptional regulators, including the fimbria-associated regulators PapB and FocB. TosR regulates the tos operon and other genes involved in adhesion (P, F1C, and Auf fimbriae) and biofilm formation.

28Other fimbrial gene clusters are found in ExPEC PAIs (Table 2). F1C/S fimbriae promote adhesion to primary human renal proximal-tubular cells by binding to α-sialyl-2-3-β-galactoside. The F1C/S fimbriae are frequently found in NMEC and could also be involved in the crossing of the blood-brain barrier, as occurs with type 1 fimbriae. However, in a meningitis animal model, the deletion of the F1C/S fimbriae operon in E. coli K1 did not significantly affect bacterial binding to and invasion of HBMECs, and the deletion also did not affect bacterial penetration into the central nervous system. Ygi fimbriae promote renal tropism, biofilm formation and in vivo fitness in the urine and kidneys. Yad fimbriae are necessary for adhesion to a bladder epithelial cell line and biofilm formation, and the deletion of these fimbrial genes has been shown to increase motility. A double deletion strain, Δygi Δyad, showed impaired colonization of the urine, bladder, and kidneys in a mouse model, demonstrating that these fimbriae contribute to uropathogenesis. ExPEC also produces non-fimbrial adhesin FdeC that is highly prevalent in both commensal strains and InPEC. This adhesin binds epithelial cells and, collagen V and VI, and in vivo experiments suggest that it has a role in UTI infections.

29Overall, PAIs provide the ability to produce multiple fimbria that confer a selective advantage within particular niches and are involved in crosstalk between regulators of various fimbrial types and in motility.

Invasins Encoded by PAIs

30Extraintestinal E. coli PAIs can produce invasins, which are structures that promote bacterial internalization within host cells. This process allows bacteria to escape targeting by the immune system and is involved in the formation of the IBCs observed in UTIs and Crohn’s disease with UPEC and AIEC, respectively. These internalizations also allow bacteria to cross epithelial and endothelial barriers by transcytosis.

31The PAI-encoded protein Hek/Tia, which is more frequently found in UPEC than in commensal isolates (Table 1), is responsible for bacterial aggregation on the surface of enterocytes, as is observed for enteroaggregative E. coli, and is involved in the invasion of T84 enterocytic cells. The mechanism behind the invasion of enterocytes and the bladder epithelium and the requirement for preliminary adhesion are still largely unknown. The invasion of HBMECs by NMEC requires binding to and the invasion of the HBMECs and involves the PAI-encoded microbial factors IbeA and cytotoxic necrotizing factor-1 (CNF-1), as well as the housekeeping factors OmpA, NlpI and arylsulfatase-like gene aslA. Studies have attempted to identify the receptors at the surface of HBMECs for these bacterial factors: CNF-1 interacts with laminin receptors, while IbeA binds to both vimentin and HBMEC contactin-associated protein 1 (CASPR1). This latter interaction activates focal adhesion and kinase signaling, which causes E. coli internalization.

Toxins Encoded by PAIs

32Extraintestinal E. coli strains can use PAI-encoded genes to produce several toxins, including α-hemolysin, CNF-1, cytolethal and distending toxin (Cdt), and colibactin (Clb).

33E. coli α-hemolysin is a pore-forming toxin belonging to the class of RTX toxins that are encoded by the hly operon. Although most UPEC strains carry one copy of the hemolysin operon, pyelonephritogenic strains 536 and J96 harbor two copies of the hly operon and both loci are required for full virulence. The toxin is encoded by the hlyA gene. The gene hlyC encodes an acyl transferase that is required for activation of the toxin, while genes hlyB and hlyD are involved in the energy-dependent secretion of HlyA. HlyA is a hemolysin that targets different cell types, including leukocytes. The involvement of α-hemolysin in virulence has been suggested because of its high prevalence in UPEC compared to its prevalence in fecal strains and because of its high expression within IBCs. In upper urinary tract infections, α-hemolysin is thought to play both a direct cytotoxic role in renal cells and a pro-inflammatory role that causes the secretion of cytokines IL-6 and IL-8 and alters Ca2+ membrane flow. The combination of these two actions weakens the renal epithelium and promotes the passage of bacteria into the blood. By interfering with NF-kB-mediated proinflammatory signaling pathways and triggering the breakdown of paxillin and other host regulatory proteins, HlyA also dampens the host’s immune response to infection and enhances the exfoliation of bladder epithelial cells.

34CNF-1 induces the formation of giant multinucleated cells, changes actin and tubulin organization, and most likely promotes cell spreading. Its toxic activity arises because of the post-translational activating mutation of Rho GTPases by deamidation, an essential control factor in the shape, adhesion, mobility, phagocytosis, and oxidative burst in host cells. The CNF-1 prevalence is higher in strains isolated from UTIs and prostatitis than in fecal isolates. An animal model of UTIs has shown an involvement of CNF-1 in resistance to phagocytosis by macrophages and in the induction of deep and persistent infections of the bladder. The CNF-1-mediated disruption of the Rho GTPase signaling pathways has antiapoptotic abilities in uroepithelium cells and leads to immune dysregulation, while conferring a survival advantage in the presence of neutrophils. CNF-1 also plays an important role in the rearrangement of the HBMEC cytoskeleton that allows crossing of the blood-brain barrier.

35The pks genomic island present in E. coli strains of phylogroup B2 encodes colibactin, a hybrid polyketide/non-ribosomal peptide that causes DNA damage and cell cycle arrest of eukaryotes. The colibactin-encoding determinant has been detected primarily in extraintestinal pathogenic isolates of E. coli, other Enterobacteriales, and commensal E. coli. E. coli persisting in the infant gut microbiota tends more often to carry the pks island than do either intermediate-term colonizers or transient strains, suggesting that the pks island contributes to the gut-colonizing capacity of group B2 strains. The frequent detection of the pks island in E. coli isolated from biopsies of patients suffering from colon cancer also suggests involvement in long-term intestinal colonization, raising the question of its role in colorectal cancer. The presence of the pks island in ExPEC could also indicate that colibactin contributes to fitness or virulence during extraintestinal infections. A recent observation indicates that the probiotic effects of the E. coli Nissle 1917 strain to ameliorate colitis severity and to modulate cytokine expression cannot be separated from the strain’s ability to express functional colibactin. This finding demonstrates that, depending on the niche or context, colibactin-producing PAI can be considered either a virulence factor and/or a probiotic factor.

36The TLRs are an important family of innate sensors that recognize diverse microbial products and launch the signaling pathways that ultimately lead to the clearance of the pathogen from the host and the establishment of a memory response in anticipation of any subsequent attack. ExPEC PAI-serU can interfere directly with the TLR signaling pathway by producing an inhibitor homolog of TLR receptors to dampen the NF-kB-induced proinflammatory response. This protein, called the TLR domain containing-protein C (TcpC), is secreted by an efflux pump and then internalized into macrophages, where it can disable TLR signaling through direct binding of MyD88, the key TLR signaling adaptor. The tcpC gene is present in 30–40% of the E. coli strains isolated from septicemia and in almost half of those causing pyelonephritis but is less common in cystitis (22%), ABU (16%) and E. coli strains of the fecal microbiota (8%) (Table 1). TcpC can increase the severity of UTIs in humans, which is consistent with the important role of TLR4 in host defense in the urinary tract.

Autotransporters Encoded by PAIs

37Extraintestinal E. coli PAIs encode several autotransporters (ATs), which are a family of proteins that mediate their own secretion through the outer membrane of gram-negative bacteria. Also known as the type V secretion system, ATs contain a C-terminal membrane anchor region that forms a pore through which the passenger domain is translocated to the cell surface. The passenger domain of ATs is exposed at the bacterial surface and/or secreted after autoproteolytic cleavage. The AT passenger domains are diverse in function and can act as enzymes (lipases, esterases, or proteases) and/or as adhesins.

38AT antigen 43 (Ag43) is widely distributed among E. coli strains, including UPEC. Ag43 mediates autoaggregation, biofilm formation and host cell adhesion. It promotes persistent colonization in a UTI mouse model and is expressed within IBCs, suggesting that Ag43 plays a role in the intracellular growth phase of UPEC. Ag43 is also involved in the adhesion of NMEC to HBMECs. The ATs UpaB and UpaC and their homologs are found in UPEC and in various strains of E. coli. UpaC promotes adhesion to abiotic surfaces and biofilm formation, while UpaB promotes adhesion to components of the extracellular matrix. In vivo experiments have suggested that UpaB, unlike UpaC, is important for bacterial fitness during UTI. Finally, the UpaG autotransporter promotes UPEC biofilm formation on abiotic surfaces and facilitates binding to extracellular matrix proteins, fibronectin, and laminin.

39Extraintestinal E. coli PAIs also produce serine protease ATs that are designated as SPATEs (serine protease autotransporters of Enterobacteriaceae) such as Tsh (temperature sensitive hemagglutinin), Hbp (hemoglobin binding protein or hemoglobin protease), Sat (secreted autotransporter toxin), Vat (vacuolating autotransporter toxin) and Pic (protein involved in colonization, cf. DEC section). Tsh was initially identified in APEC as a mannose-resistant hemagglutinin that is overexpressed at low temperatures. It is observed in more than half of APEC isolates and its prevalence increases in high-lethality isolates. The association of Tsh with the virulence of APEC isolates was further reinforced by the detection of fewer and less pronounced lesions in the air sacs of chickens infected with a Tsh mutant than with the wild type strain. The tsh gene is also observed in UPEC (4.5%) and NMEC (11.5%). Frequently encoded by plasmids, tsh is also observed in PAIs and promotes adhesion to red blood cells, hemoglobin, and the extracellular matrix proteins fibronectin and collagen IV. Tsh only differs from Hbp by two amino acid residues in the passenger domain and probably shares functional similarities regarding the breakdown of hemoglobin factor V and mucin.

40The vat gene was also originally identified in an APEC strain as a cytotoxin of chicken embryonic fibroblast cells that contributes to avian cellulitis infection. It only shares 78% identity with Tsh and has different proteolytic activities. However, Tsh/Hbp, Vat and Pic have mucinolytic activity and enhance epithelium colonization. Pic and Tsh/Hbp also target the major human leukocyte-adhesion molecules CD43, CD44, CD45, and CD93, thereby deregulating leukocyte migration and inflammation. The Sat ATs induce vacuolation of renal cells, and the serine-protease activity is required for the cytopathic effects of Sat. Mention has been made of the role of Sat in breaking down the glomerular barrier to allow bacteria to pass into the blood. Three new SPATEs—Sha (Serine-protease hemagglutinin autotransporter), TagB and TagC (tandem autotransporter genes B and C)—have also been recently reported in an ExPEC O1:K1 strain isolated from turkeys and they induce cytopathic effects on a bladder epithelial cell line. However, their functional role in UTIs remains to be investigated.

41SslE/YghJ (secreted and surface associated lipoprotein) is secreted by the type II secretion system and probably anchored at the bacterial surface in ExPEC. It is a metalloprotease domain initially observed in NMEC. SslE, which is widely found in both commensal and pathogenic E. coli, has greater prevalence in pathogenic isolates than in commensal isolates, which may also be defective for its secretion. SsIE targets mucins and has cytotoxic and proinflammatory activities that can promote the virulence of pathogenic E. coli and sepsis in neonates.

Iron Uptake Systems Encoded by PAIs

42Iron is essential for bacteria because of its involvement in many metabolic functions, such as the transport of oxygen and electrons. Most iron in humans is complexed with the transport molecules transferrin (found in blood) and lactoferrin (found in the digestive tract and in salivary and pulmonary secretions) and with reserve molecules (ferritin) or it is incorporated into the heme of hemoglobin and myoglobin. This complexation results in iron limitation, which in turn serves as one of the innate defenses against the survival of bacteria within hosts. To use iron from the host, bacteria have developed capture systems that target these iron-complexed forms within citrate ions (fec system) or heme (chu system). The bacteria use transport systems (Sit systems) or produce PK-NRP compounds called siderophores that capture iron from host transporters or reserve systems and transport it back to the bacteria via specific receptors. The siderophore enterobactin, which is found in almost all E. coli, is encoded by the core genome. Thus, Lipocalin-2, which is capable of sequestering enterobactin, prevents its uptake by the bacteria via the FepA receptor. Therefore, ExPEC acquires PAI-encoding iron-uptake systems to thwart this host defense mechanism. The most frequently encountered siderophores are aerobactin, yersiniabactin and salmochelin. Salmochelin is a modified enterobactin formed by glycosylation of one of its derivatives, and this modification makes salmochelin insensitive to inhibition by human Lipocalin-2. The PAI-encoded siderophores salmochelin and yersiniabactin are both produced at significantly higher levels in ExPEC isolated from urine than in rectal isolates and could play a greater role than enterobactin within the human urinary tract. In a newborn rat meningitis model, salmochelin plays an important role in maintaining a high level of bacteremia, which is a necessary step for bacterial crossing of the blood-brain barrier.

43Receptor-mediated uptake of heme is another iron-uptake mechanism encoded by ExPEC PAIs. Two outer membrane heme receptors, ChuA and Hma, have been characterized, and both were required for bacterial optimal fitness in a UTI mouse model. ChuA could also be important during IBC formation. The PAI-encoded Sit system transports manganese and ferrous iron into the ExPEC cytoplasm and enhances resistance to oxidative stress. The sit genes are upregulated during murine UTI and are expressed during human UTIs, suggesting that they contribute to urofitness.

Protectins Encoded by PAIs

44Protectins are the bacterial elements that protect bacteria against host weapons, such as antibacterial factors (i.e., defensins, D-serine), serum complement and immune cells. PAI-encoded capsules (K antigen) confer this type of protection. The resulting homopolymers exposed at the surface of the bacteria can resemble various glycoconjugates found within vertebrate hosts (e.g., sialic acid), such as K1 or K4 capsules, and can contribute to the bacterium’s immune-evasion strategy. The K1 antigen is associated with E. coli strains responsible for invasive infections. K1 antigen is critical for the induction of a high degree of bacteremia, is associated with a high resistance to both phagocytosis and serum and is required for bacterial crossing of the blood-meningeal barrier. Other PAI-encoded protective elements are surface proteins such as the Iss (increased serum survival) protein, which has been implicated in serum resistance.

45D-serine is a bacteriostatic amino acid that is found in urine and brain. UPEC and NMEC strains frequently harbor a PAI-encoded dsdCXA locus. The dsdCXA locus has been hypothesized to be important for UPEC/NMEC pathogenesis. Accordingly, isogenic strains defective in dsdA have a growth defect in urine relative to dsdA wild-type strains. However, dsdA neither positively nor negatively affected the ability of UPEC strain CFT073 to colonize urinary tract in a mouse model.

Diarrheagenic Escherichia coli

46As already described above, InPEC essentially regroups (i) E. coli strains responsible for diarrhea (DEC) and (ii) AIEC, which are not involved in diarrhea but are involved in inflammatory bowel diseases. Among the DEC, the EPEC and EHEC have undoubtedly received the most attention regarding PAIs. By contrast, little information is available about PAIs in DAEC, except for the presence PAI pic/set in some strains. Compared to ExPEC, the information about PAIs in DEC is generally scarce.

Physiopathology of DEC

47The six recognized categories of DEC are primarily based on clinical symptoms, including the type of diarrhea, related syndromes or bacterial interaction with intestinal epithelium cells, as revealed by histological observation, as well as associated molecular determinants of virulence and pathogenicity.

48ETEC are mainly associated with traveler’s diarrhea but can also appear in weanling diarrhea among children. The diarrhea is generally not bloody, but watery, and is sometimes associated with vomiting that ranges from mild to severe purging, similar to cholera. The pathogenicity of ETEC mainly relies on heat-labile (LT) and/or heat-stable (ST) enterotoxins responsible for net secretion of intestinal fluid. The LT form belongs to the cholera toxin family and is secreted by a Type II secretion system (T2SS) with the contribution of LeoA (labile enterotoxin output) for its efficient secretion. Unlike the cholera toxins that are secreted as free soluble proteins, LT associates with the outer membrane vesicles by binding to LPS. Sequence divergences confer LT with the ability to bind different ganglioside receptors that mediate entry into the host cell. LT is an ADP-ribosylating toxin that inactivates the GTPase activity of the G protein complex by ADP-ribosylation. This, in turn, leads to prolonged activation of the adenylate cyclase activity and elevated intracellular levels of cAMP and results in electrolyte release and stimulation of intestinal excretion.

49By contrast, ST can be discriminated into type I (ST-I, also called STa) and type II (ST-II, also called STb), which target different receptors. ST-I binds and activates a guanylate cyclase C receptor and the subsequent increase in intracellular cGMP levels activates the cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel. ST-II, which binds the acidic glycosphingolipid sulphatide, activates a pertussis toxin-sensitive GTP-binding regulatory protein that ultimately activates CFTR. Surface colonization is an important in step in the physiopathology of ETEC, which involves a large and diverse repertoire of pili, called colonization factor antigens (CFAs), that bind to different receptors at the surface of the host cells. Additional virulence factors can be present, including some surface-exposed adhesins like TibA or Tia, the toxins ClyA and EAST1, or the protease EatA; however, this does not appear to be a common feature among ETEC.

50Enteropathogenic E. coli infection manifests as acute diarrhea in infants and is often associated with vomiting. A hallmark of EPEC is its attaching and effacing (A/E) histopathology. This is accompanied by intimate attachment of bacterial cells expressing cell-surface intimin (Eae) with the cognate translocated intimin receptor (Tir) expressed on the surface of eukaryotic cell following injection into their cytosol by a Type III, subtype a, secretion system (T3aSS). A large repertoire of T3aSS effectors participate in the infection process. However, and despite decades of investigations, the exact molecular mechanisms by which EPEC induce diarrhea are not well understood.

51The presence or absence of plasmid pEAF (E. coli adhesion factor) encoding bundle-forming pili (BFP), can be used to further categorize EPEC into typical (tEPEC) and atypical EPEC (aEPEC), respectively. In tEPEC, BFP are type 4 pili (T4P) encoded by the bfp operon and induce a localized adhesion (LA) pattern at the surface of intestinal epithelial cells to form microcolonies. In aEPEC, no localized LA patterns are observed but instead localized-adhesion-like (LAL) or aggregative adhesion (AA) patterns form loose bacterial cell clusters involving alternative adhesion factors such as EspA filaments, LifA (lymphocyte inhibitory factor) or ECP (E. coli common pili).

52EHEC are responsible for pediatric diseases ranging from watery diarrhea to hemorrhagic colitis that can further lead to thrombotic microangiopathies, especially hemolytic and uremic syndrome (HUS) and thrombotic thrombocytopenic purpura (TTP). EHEC are primarily characterized by the expression of the key virulence shigatoxin (Stx) that binds to glycosphingolipid Gb3 before penetrating the cell and inducing apoptosis of kidney epithelial cells. Nonetheless, and somewhat surprisingly, the way that Stx crosses the intestinal mucosa is not fully elucidated. While tEHEC possess the locus of enterocyte effacement (LEE) as in any EPEC, the aEHEC do not express the injectisome and instead rely on alternative monomeric and multimeric surface colonization factors, such as adhesins and pili. Similar to EPEC, however, the precise molecular mechanisms that induce bloody diarrhea remain elusive.

53Of note, and contrary to EPEC, A/E lesions are never detected on colonic biopsies from EHEC infection, although they are observed in vitro using epithelial cell culture or human colonic explants. Some E. coli isolated from other sources (environment, animals, food and the agri-food chain) can be genotypically characterized as possessing the stx gene and can be regrouped as shigatoxin-encoding E. coli (STEC, or shigatoxin-producing E. coli when Stx production has been experimentally confirmed). The pathogenicity of EHEC has been clinically ascertained, and STEC can have very different virulence levels, up to hyper-virulence; alternatively, they can even be potentially avirulent and are not systematically and rigorously considered as pathogenic. With more than 400 distinct serotypes revealed in STEC, only a handful of EHEC serotypes has been clinically associated with epidemic outbreaks, although they can all be potentially involved in human infections. The search for genetic markers has been the Grail for decades; nevertheless, the differences in the virulence level is most certainly associated with various compensatory combinations of molecular determinants, including PAIs, together with regulatory networks that can act at various levels and not just regarding the transcriptional aspects of genetic expression.

54After ETEC, EAEC is the second most common cause of tourista that causes acute and sometimes persistent diarrhea. The diarrhea is typically non-bloody and without inflammation or emetic symptoms. Upon adhesion onto intestinal epithelial cells, EAEC exhibit a typical stacked brick-like pattern known as aggregative adhesion. EAEC form a loose biofilm at the mucosal surface involving pili and especially AAF (aggregative adhesion fimbriae, encoded by a large plasmid pAA). Additional virulence factors include the pAA-encoded enterotoxins EAST1 and Pet (plasmid-encoded toxin) and the chromosome-encoded ShET1 (Shigella enterotoxin 1) and Pic (protein involved in intestinal colonization). However, the molecular basis that mediates diarrhea upon infection by EAEC remains to be elucidated.

55Extensive and compelling evidence indicates that EIEC and Shigella cannot be discriminated from a taxonomic point of view as they both belong to the same species, E. coli, and basically correspond to the same pathotype. Differences in the virulence level have sometimes been put forward, with Shigella considered hyper-virulent relative to EIEC, which generally induces less severe disease. Nevertheless, significant exceptions do exist and the intestinal illness is in fact indistinguishable. As with EHEC/STEC, the search for genetic markers that could solely explain the difference in the virulence levels might have somehow hindered the potential in the heterogeneity of the regulation of the genetic expression from one EIEC/Shigella strain to another as well as between individual cells in an isogenic population. The hallmarks of EIEC/Shigella are the invasion plasmid pINV, which encodes a T3aSS (like in EPEC and EHEC), and cognate effectors of key importance in host invasion of this intracellular pathogen, which have been extensively investigated. Additional virulence factors include the mucinase Pic (previously called ShMu for Shigella haemagglutin and mucinase), also found in EAEC and contributing to the initial step of intestinal colonization. Another is the protease SigA (Shigella immunoglobulin A protease) that contributes to the watery prodromal phase of the infection, together with the enterotoxins ShET1 and ShET2. The exact function and mode of action of the latter enterotoxins remain to be determined.

56DAEC, rather than DEC, are essentially involved in diarrhea in young children, and some DAEC are ExPEC involved in urinary tract infections. EIEC are known to preferentially colonize the colon, as well as the distal ileum for EHEC, whereas the ETEC and EPEC colonize the small intestine, as well as the colon for EAEC. By contrast, the primary site of intestinal colonization of DAEC has not been determined. DAEC exhibits a scattered adhesion pattern on intestinal epithelial cells, called diffuse adhesion. DAEC have previously been subdivided into those expressing and those not expressing Afa/Dr (afimbrial adhesin/decay-accelerating factor receptor) adhesins, but the latter subclass is now considered to belong to aEPEC, leaving Afa/Dr adhesins as a hallmark of DAEC. Previously, daaE and/or daaC were briefly suggested as biomarkers of DAEC, but F1845 pili are rare among DAEC strains. The group of Afa/Dr adhesins is quite diverse and can induce rearrangement of the cytoskeleton upon binding to the eukaryotic cell and can even promote bacterial internalization. Additional virulence factors include pks island products (such as colibactin), flagella and the secreted autotransporter toxin (Sat). Notably, while some DAEC strains have been sequenced, no complete assembled genomes are as yet available. In addition, contrary to EHEC, ETEC, ETEC or EAEC with E. coli strains EDL933, H10407, E2348/69 or O42, respectively, no prototypical DAEC strains have been sequenced to date, which limits our understanding of PAIs within this DEC pathotype.

PAI-Encoded Virulence Factors of DEC

57While PAIs were originally described in ExPEC, they later appeared to be present in InPEC. To date, 13 PAIs have been characterized, some of which are present in different DEC pathotypes (Figure 4; Table 3).

LEE PAI

58The LEE is undoubtedly the most investigated PAI in DEC, and readers are referred to recent reviews solely dedicated to this PAI for detailed information. The presence of the LEE is the hallmark of EPEC, but it is also found in tEHEC. As first described in prototypical EPEC strain E2348/69 and EHEC strain EDL933 (or strain Sakai), the core region of the PAI consists of 41 CDS, organized into (i) 5 operons named LEE-1 to LEE-5 encoding transcriptional regulators, including the master LEE-encoded regulator (Ler), chaperones and structural components of the T3aSS, as well as some effectors, (ii) one grlAB bicistron encoding transcriptional regulators, and (iii) some monocistrons. The T3aSS constitutes a major virulence factor that allows the assembly of a cell-surface needle structure, the injectisome which enables the direct transport of virulence effectors into a host cell. The regulation and function of the T3aSS has been extensively reviewed and readers are invited to consult the above reviews for further details on this vast field of research. Apart from the LEE core region, a certain degree of variability exists between LEEs found in different EPEC or EHEC strains, with estimated sizes ranging from approximately 35 to 110 kb. The LEE core region can be completed by prophage genes, as in EHEC ELD933 or Sakai, whereas the fusion with OI-43/48 SpLE1 (Sakai prophage-like element 1) containing a ter operon conferring resistance to tellurite. The OI-122 SplE3, which contains a lifA (efa1) region that encodes the virulence factor lymphostatin or some truncated versions of these islands, can also be found in some variants of the LEE PAI. The LEE PAI is inserted at the selC tRNA gene, but the pheV and pheU tRNA genes were also reported as alternative insertion sites for the LEE among these variants. Phylogenetic analyses based on selected genes indicated that the LEE core region could be discriminated into two main core clusters, one harboring eae-α and -γ and the other one harboring eae-β and -ε independently from the tRNA insertion site. Nonetheless, the current knowledge on the distribution of the LEE indicates complex events of genetic acquisitions and recombinational rearrangements in E. coli genomes and the evolutionary network remains quite incomplete.

PAI pic/set

59PAI pic/set is inserted into the pheV gene and was identified in EAEC, as well as in EIEC/Shigella (called SHI-1 PAI, previously also known as She) and UPEC (called PAI IICFT073), and in some EHEC and EPEC strains; however, they differ in size, organization, gene composition and genome localization. Island probing revealed this PAI to be quite unstable and to undergo spontaneous excision from the chromosome at a frequency of 10–5–10–6. The number of IS-like element flanking the gene (especially IS629 and IS911 also found around other genes encoding SPATEs) suggested that pic (previously called she) is part of a mobile element that could move independently of the PAI.

60The setB and setA genes, contained within the pic gene but on the complementary strand, encode the oligomeric enterotoxin ShET1. The transcription of setB was induced in simulated human intestinal microbial systems, although the exact signals promoting gene expression remain to be identified. The genetic expression of the pic/set locus is quite unusual and complex. At least three promoters, Ppic1, 2, and 3, could direct the transcription of pic but with different forces and constitutive/inducible expression. A PsetA promoter was identified just upstream of setA, whereas a PsetB was identified 1.5 kb upstream of setB with a silencer DRE (downstream regulatory element) region in between that repressed the expression of setBA without any effect on pic transcription. Apart from regulation by its own promoter, setA also appears to be expressed by transcription readthrough from setB.

LAA PAI

61The locus of adhesion and autoaggregation (LAA) is a composite 86-kb PAI composed of four modules. In addition to the host immune response suppressor SisA (shiA-like inflammation suppressor genes A), module I encodes an autoaggregative factor with Hes (hemagglutinin from STEC), a member of the Hra (heat-resistant agglutinin) family, together with module IV encoding Ag43, a member of the SAAT (self-associating autotransporter). Module III encodes the adhesin Iha (iron regulated gene homolog adhesin) and the SPATE LesP (LAA encoded SPATE), while module IV actually corresponds to the PAI previously known as PAI ICL3.

62PAI ICL3 was originally described is a hybrid genomic region that entered B1 E. coli genomes on multiple and independent occasions. It is composed of segments of the EHEC EDL933 OI-48 at both its extremities and two genomic segments, GS-I and GS-II. GS-I comprises fragments of the Z1640 gene encoding the ShlB transporter family of the T2bSS, whereas GS-II is a composite region encoding a member of the ShlA exoprotein family secreted by the T5bSS. GS-II encodes several transposase genes, genes originating from EHEC EDL933 OI-122, including pagC (phoP-activated gene C), and an additional fragment of Z1640. Different variants and often deleted versions of PAI ICL3 were reported as carried by genomic islands, including GIpheV-CRICC168 from Citrobacter rodentium, inserted at pheV, selC or serW tRNA. However, the mosaic structure of LAA PAI was not understood at the time, and these GIs represent complete and incomplete LAA PAI variants (i.e., comprising less or all of the four modules) belonging to two major lineages. In agreement with PAI ICL3 investigations, the LAA PAI appeared to be exclusively present in aEHEC in the first instance and could thus complement the absence of the LEE PAI.

PAI iuc/iut

63The SHI-2 (Shigella pathogenicity island 2) PAI is inserted at the selC tRNA gene and encodes an integrase almost identical to its ortholog in the LEE PAI. It possesses an abundant number of partial transposases and IS elements, which are remnants of the multiple stepwise assembly of the island. SHI-2 codes the iucABCD operon for synthesis of aerobactin, a hydroxamate siderophore involved in iron uptake, as well as the iutA gene encoding the outer membrane receptor for aerobactin complexed to iron. In Shigella boydi, a 21-kb PAI harboring the iucABCD-iutA operon but inserted at the pheU tRNA gene and possessing LEE prophage genes absent from SHI-2, was called SHI-3. Nonetheless, SHI-3 remains very closely related to the 30-kb SHI-2 in S. flexneri 2a SA100 and 23.8-kb SHI-2 in S. flexneri 5a M90T, and it belongs to the same family of PAI.

64The iut/iuc operon is highly conserved in SHI-2 and SHI-3 and can be inserted in at different loci, especially in paralogs to phe tRNA genes (at least pheV, pheR and pheU). The SHI-2/3 PAIs are regrouped under the designation of PAI iuc/iut to rationalize the naming in a way similar to that of PAI pic/set. The PAI iuc/iut would have been acquired horizontally, as suggested by its high degree of conservation with the aerobactin operon harbored on plasmid pColV in some E. coli strains. SHI-2 also harbors several genes encoding the predicted cytoplasmic proteins, including ShiA (a putative quinone reductase) that has been demonstrated to attenuate inflammation by abrogating the innate T-cell response. SHI-2 also encodes potential integral inner membrane proteins, including ShiF (a putative transporter of the major facilitator superfamily), a dispensable auxiliary factor involved in aerobactin export and/or synthesis. Like pic/set in SHI-1, the shiF and shiG genes overlap and would be transcribed in reverse orientation on a complementary strand, although experimental investigations of genetic regulation and function of ShiG are still required.

65Importantly, the imm (immunity) gene (shiD) appears responsible for immunity to different colicins, including colicin V (ColV). Colicins are proteins produced by E. coli that cause cell lysis of sensitive strains. In some strains, PAI iuc/iut is inverted and is thus expressed from the complementary strand, but it is also differently localized and orientated with respect to the oriC, which could have consequences on the expression of the aerobactin genes. Among the EIEC/Shigella, the size of PAI iuc/iut clearly differs, but further investigations are needed to determine their diversity and to gain insight into their gene content, organization, phylogeny and distribution in E. coli genomes.

PAI tia

66The prototyptical ETEC strain H10407 contains 25 identified ROD (regions of difference) on the chromosome, two of which are currently viewed as PAIs with the tia (toxigenic invasion locus A) and tibA (toxigenic invasion locus b) loci. Tia is encoded in a large PAI of approximately 46 kb (corresponding to ROD-20), inserted at selC and flanked by direct repeat sequences (DRSs) identical to those found in PAI-1 in UPEC. Tia can act both as an invasin, contributing to the invasion of intestinal epithelium cells, and as an adhesin, binding to heparin-sulfate proteoglycans. In addition to int, PAI tia encodes LeoA, an accessory factor for efficient LT secretion, as well as three additional CDS encoding a putative transport system. DNA hybridization studies indicate that PAI tia is present in numerous ETECs, as well as in some EPEC and EHEC. Nonetheless, the hybridization and multiplex PCR patterns exhibit a considerable level of heterogeneity, suggesting differences in the organization and content of the PAI. The spread of this PAI among DEC and broadly among E. coli strains requires further in-depth investigations.

PAI tibA

67In ETECs, TibA is encoded on a 21.6-kb PAI of low G + C content, corresponding to ROD-13. Besides the tibDBCA operon, the PAI tibA codes 14 transposases and IS66, but the insertion site remain elusive. TibA belongs to the SAAT family, is secreted by the T5aSS and is glycosylated by the heptosyltransferase TibC. Besides cell aggregation and biofilm formation, this glycosylated surface protein is involved in adhesion to intestinal epithelial cells. The functions of TibD and TibB are yet to be determined. The prevalence and distribution of PAI tibA among E. coli remains to be investigated, though it is not systematically present in all ETEC strains.

SE PAI

68LEE-negative EHEC (i.e., aEHEC) contain an 8-kb PAI coding a subAB2 operon, expressing a subtilase. The subAB1 allelic variant is located on a plasmid, but no additional DNA regions corresponding to the PAI were identified, suggesting that it was not horizontally acquired from this plasmid. SubAB is a well-known cytotoxin inducing apoptosis in LEE-negative EHEC. This so-called SE (subtilase encoding) PAI also harbors genes encoding an integrase and a sulfate, as well as Tia and ShiA, and is inserted in the pheV tRNA gene. The presence of tia, as in PAI tia, and shiA, as in PAI pic/set, as well as the presence of subAB2 in some ETEC strains, raises questions about the origin and evolution of the SE PAI with respect to these PAIs and demands further phylogenetic analyses. The SE and LEE PAIs have the same insertion site, which led to the hypothesis that mutual exclusion events could have occurred between these PAIs competing for integration at pheV tRNA gene and could further explain the strong association of the subAB2 operon with aEHEC versus tEHEC. As with PAI iuc/iut, however, insertion at homologous phe tRNA genes cannot be excluded. In general, the prevalence of SE PAI in DEC, and in other E. coli, requires further in-depth investigations.

SHI-O PAI

69The SHI-O PAI harbors genes involved in serotype conversion in Shigella by modification of the structure of the O-antigens. SHI-O would appear to be a remnant of an ancient lysogenic bacteriophage that lost its ability to enter into a lytic cycle and underwent excision upon deletions and mutations of sensitive key regions. This PAI codes three genes, gtrA, gtrB and gtrV, that express the glycosyltransferases that allow differential glycosylation of bacterial lipopolysaccharide (LPS) and thus antigenic conversion. SHI-O was also shown to be inverted and to differ in its composition between Shigella strains and to have a different insertion in E. coli. Besides the generation of a large variety of shigella serotypes, the modification of O antigens by SHI-O contributes to the modulation of the virulence level through different abilities to evade the host immune response. The prevalence and distribution of SHI-O in E. coli has not been questioned as yet, although it is present in prototypical EAEC O42.

SRL PAI

70The SRL (shigella resistance locus) PAI is a 66-kb element containing at least 59 CDS, especially a 16-kb cluster designated as SLR with genes conferring resistance to streptomycin (aadA1), ampicillin (oxa-1), chloramphenicol (cat) and tetracycline (tetABCD-R). The SRL PAI is a mobile element flanked by short 14-bp DRSs, which can excise from its insertion site at tRNA gene serX. The deletion rate was estimated at approximately 10–5 and led to antibiotic-sensitive variants. The int (integrase) gene was required for precision deletion of the island through site-specific recombination. Alternative insertion sites are strongly suspected, such as serW, paralogous to serX, and orf58. Besides antibiotic resistance, SRL PAI encodes a functional ferric dicitrate iron transport system (fecABCDE-I-R), which expands the battery of iron acquisition mechanisms in the bacterial strain. Functional genetic analysis revealed that SRL PAI encoded a functional aspartate racemase converting D- to L-aspartate (AspR) and a cognate transporter (DcuA) enabling catabolism of D-asparate as a sole carbon source. Catabolism of D-amino acids, such as D-aspartate, as a carbon source and/or detoxification of their toxic effects on protein biosynthesis could have an important role in the colonization of the human gastrointestinal tract, an aspect which undoubtedly requires further in-depth investigations. The aspR and dcuA homologs are present in some EHEC O104:H4 isolates, but the broad prevalence of SLR PAI in E. coli needs thorough investigation, although this PAI seems quite prevalent in multiresistant Shigella isolates.

PAI espC

71EspC (E. coli secreted protein C) is a SPATE with enterotoxic activity that induces necrosis and apoptosis in epithelial cells. EspC actually cleaves the cytoskeletal actin-associated protein α-fodrin, the focal adhesion protein paxillin and a focal adhesion kinase (FAK). In EPEC, EspC is encoded in a 15.2-kb PAI inserted between orf360 and tRNA ssrA genes, with respect to the E. coli K12 chromosome. Besides five putative transposases, PAI espC encodes an integrase and a homolog to the virulence effector VirA from S. flexneri that is involved in cellular invasion and intracellular spreading, as well as additional CDS with low sequence similarities that require further analysis to ascertain their function. The low G + C content of this PAI suggests it was acquired horizontally. PAI espC seems present in other DEC, including some EPEC and EHEC, but additional studies are needed to characterize the possible diversity in the genetic organization, prevalence and distribution.

PAI sit

72Originally described in S. flexneri, the PAI sit primarily harbors a sitABCD locus encoding a ferrous iron and manganese uptake system belonging to the ABC transporter family, homologous to Sit (salmonella iron transport). The PAI sit is found in some commensal and pathogenic E. coli at various chromosomic locations, with insertion sites that fall into four clusters. The size and orientation of the island seem to differ considerably, but further studies are required to assess the degree of variation in the gene content of the PAI sit. This PAI has multiple chromosomic insertion sites and a large number of genetic rearrangements and deletion and insertion sequences that would occur through homologous recombination rather than site-specific mechanisms. Therefore, this PAI appears quite unstable.

HPI

73The HPI (high pathogenicity island) is a PAI originally identified in Yersinia enterocolitica. It later appeared widely disseminated in Enterobacteriaceae, including DEC, but essentially in EAEC, as it is rarely present in EIEC/Shigella, EPEC, ETEC or EHEC. The HPI encodes an iron uptake system based on the siderophore yersioniabactin (Ybt), which was demonstrated to be functional in several EAEC strains and contributes to the virulence. While fyuA (also called psn) and ybtPQ (also called irp6-7) encode an outer-membrane receptor and an inner-membrane transporter for iron uptake, respectively, irp1-5 and ybtS (irp9) are involved in the biosynthesis of Ybt. In E. coli, the HPI is approximately 40 kb and is generally inserted at the asnT tRNA gene. However, it can also be inserted at the asnV tRNA gene harbored in place of IS600, an additional 34.4-kb fragment constituting a functional ICE encoding a T4SS. This element could constitute the progenitor and missing link that would explain the dissemination of HPI among E. coli.

PAI IIAL862

74Escherichia coli AL862 was primarily isolated from a septic patient with a cancer, but the PAI containing the afa-8 operon, and further called PAI IIAL862, appeared as a preferential insertion into the pheV gene in several human and bovine pathogenic E. coli strains. The afa-8 operon, encoding the Afa/Dr adhesin AfaEVIII, is expressed in some E. coli strains isolated from animals with diarrhea, including calves, pigs, and poultry. PAI IIAL862 can be identified in some human intestinal E. coli isolates but evidence of its implication in diarrhea in humans remains to be demonstrated. This PAI is supposedly present in some DAEC, but investigations are still required. Among the four sRNAs encoded in PAI IIAL862, the thermoregulated sRNA AfaR further regulates the expression of the AfaD-VIII subunit. PAI IIAL862 also includes the deoK operon, which confers the ability to use deoxyribose as a carbon source and would increase competitiveness with respect to host infectivity.

Conclusion

75As reviewed here above, PAIs are widespread in E. coli and are major actors in the genome plasticity of this bacterium. The PAIs have shaped the genome of this bacterial species, which further explains the emergence of different pathotypes that result in different intestinal and extraintestinal diseases. The transfer of these GIs in a one-step acquisition of the entire functional determinants, thereby enabling bacterial evolution in quantum leaps, opens the bacterium to new ecological niches. The functional elements encoded by PAIs include pathogenicity effectors, but they also contain regulatory elements involved in crosstalk between PAIs and the genetic background. This crosstalk may be key factors in genome evolution, including PAIs, and probably result in a diversity of expression profiles. This requires more investigations to obtain the best understanding of E. coli virulence and the apparent redundancy of pathogenicity factors in ExPEC.

76As exemplified by the LEE and LAA PAIs, these genomic islands are also quite plastic in terms of their insertion or deletion events, resulting in PAIs of different sizes in different strains but with a common evolutionary history. This review has further stressed that, in some cases, further investigations are still required to fill the existing knowledge gaps, for example, about the phylogenetic relationship between SE PA, PAI tia, and PAI pic/set, which may belong to the same family as was later identified for PAI ICL3 and PAI LAA. However, the PAI nomenclature is quite erratic and requires a more rigorous formulization to avoid different naming of homologous genomic islands, such as SH-2 and SH-3, found in different strains, as was done for HPI. This is necessary to facilitate PAI tracing and our understanding of their phylogeny and evolution.

77Additional PAIs are most certainly present in E. coli; examples are the genomic islands OI-57, OI-122, OI-43/48 and GI-selC17584/1 in EHEC or some ROD in ETEC. However, beyond a correlation of their association with outbreaks or severe diseases, experimental confirmation is still required to demonstrate their involvement and exact role in E. coli virulence and pathogenicity.

78The evolution of the pathogenesis of E. coli constitutes a challenge for public health authorities in both developed and developing countries. This is especially evident given the burden of disease and the emergence of new epidemic strains, as dramatically exemplified by the hybrid diarrheagenic EHEC/EAEC O104:H4 strain. The efficient horizontal gene transfer and high genetic plasticity of the E. coli genome, at both the plasmidic and chromosomic levels, represent potential public health issues that require a full comprehension and determination of the molecular mechanisms at play in the emergence of new E. coli pathotypes and/or E. coli strains with higher virulence levels. The description of numerous E. coli pathotypes with respect to intestinal and extraintestinal pathogenic diseases should not hide the fact that E. coli is primarily a normal and harmless commensal of the human intestinal microbiota, and that all these strains belong to the very same and unique bacterial species, E. coli (including Shigella).

79To avoid biases in our understanding of E. coli physiology when solely focusing on anthropocentric medical and pathogenic aspects, we must consider not only pathogenic human and animal isolates but also the commensal strains that occur all along the food chains. Very diverse selection pressures can be encountered in the trophic network and can work together to drive evolution. In the various ecosystems that E. coli calls home (e.g., animal and human GIT, food matrices and soil, water, agri-food environments), the colibiote consists of different commensal and/or pathogenic E. coli strains co-existing and interacting along with the rest of the microbiota of the biocoenoses in the various environmental conditions of the biotopes. E. coli is not intentionally pathogenic, as suggested by its pathoadaptation. However, ecocentric and biocentric views allow the perception that these different levels of interactions can induce evolution and selection of genetic traits for specific niche factors for the necessity of fitness and adaptation, which can inadvertently, collaterally, coincidentally and/or randomly result in virulence as a by-product of commensalism. Integrating the physiopathology to the ecophysiology of this species is certainly the next frontier in understanding the forces at play in the E. coli genome plasticity and the emergence of new virulence traits and/or pathotypes.

Author Contributions

80MD and RB wrote the first overall draft of the manuscript. RB drew the original pictures. MD, GD, RBe, NB, JD, and RBo wrote sections of the manuscript. RB contributed to conceptualize the overarching aims and had management as well as coordination responsibility for the execution of the work. All authors contributed to the critical revision of the manuscript, read and approved the submitted version.

Conflict of Interest

81The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Not in our library10.1128/aem.00507-09
473.
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No notice
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475.
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