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Transcriptional regulation by Ferric Uptake Regulator (Fur) in pathogenic bacteria

Bryan Troxell, Hosni M. Hassan

Frontiers in Cellular and Infection Microbiology · 2013 · Review

doi:10.3389/fcimb.2013.00059

Abstract

In the ancient anaerobic environment, ferrous iron (Fe(2+)) was one of the first metal cofactors. Oxygenation of the ancient world challenged bacteria to acquire the insoluble ferric iron (Fe(3+)) and later to defend against reactive oxygen species (ROS) generated by the Fenton chemistry. To acquire Fe(3+), bacteria produce low-molecular weight compounds, known as siderophores, which have extremely high affinity for Fe(3+). However, during infection the host restricts iron from pathogens by producing iron- and siderophore-chelating proteins, by exporting iron from intracellular pathogen-containing compartments, and by limiting absorption of dietary iron. Ferric Uptake Regulator (Fur) is a transcription factor which utilizes Fe(2+) as a corepressor and represses siderophore synthesis in pathogens. Fur, directly or indirectly, controls expression of enzymes that protect against ROS damage. Thus, the challenges of iron homeostasis and defense against ROS are addressed via Fur. Although the role of Fur as a repressor is well-documented, emerging evidence demonstrates that Fur can function as an activator. Fur activation can occur through three distinct mechanisms (1) indirectly via small RNAs, (2) binding at cis regulatory elements that enhance recruitment of the RNA polymerase holoenzyme (RNAP), and (3) functioning as an antirepressor by removing or blocking DNA binding of a repressor of transcription. In addition, Fur homologs control defense against peroxide stress (PerR) and control uptake of other metals such as zinc (Zur) and manganese (Mur) in pathogenic bacteria. Fur family members are important for virulence within bacterial pathogens since mutants of fur, perR, or zur exhibit reduced virulence within numerous animal and plant models of infection. This review focuses on the breadth of Fur regulation in pathogenic bacteria.

Full text

From PubMed Central, U.S. National Library of Medicine, as current on Oct 17, 2026. Paragraph numbers are ours, so an answer can point at one.

Introduction

Transition metals are essential elements in biological systems. Metabolic pathways, DNA synthesis, RNA synthesis, and protein synthesis are dependent on the availability of the appropriate metal cofactor. In support of this, all cells have designated gene products that transport metals to maintain cellular function; however, certain essential metals cause the formation of toxic reactive oxygen species (ROS). In the earliest description of what is now known as the Fenton reaction, iron (Fe) was shown to act catalytically in the oxidation of tartaric acid (Fenton, ). The Fenton reaction produces the hydroxyl radical (HO.), a ROS capable of oxidizing macromolecules and lipids (Imlay et al.,; Lloyd et al., ). Therefore, cells must tightly regulate the concentration of Fe to avoid ROS-mediated cell damage.

Bacteria sense their environment and alter expression of genes that promote survival. This is accomplished by transcription factors that regulate expression of beneficial or detrimental genes. In order to acquire Fe in Fe-limiting environments, bacteria and fungi synthesize and secrete low molecular weight compounds, called siderophores, which have high affinity for binding Fe3+. Most siderophores are produced by the non-ribosomal peptide synthesis (NRPS) pathway and an example is the siderophore enterochelin. The final steps of the pathway are executed by the action of the Ent proteins (encoded by the entD, entF, and entCEBA genes) (Gehring et al.,; Salvail et al., ). Aerobactin, another siderophore, is sequentially produced by the proteins IucD, IucB, IucA, and IucC (Figure 1A) that are expressed in an operon (iucABCD). Aerobactin is an example of a siderophore not produced by the NRPS pathway. Transcriptional control of both siderophores is regulated by the concentration of intracellular Fe2+ (Bagg and Neilands, ); when intracellular Fe2+ is low, the model bacterial organism, Escherichia coli induces siderophore production (Brot and Goodwin,; Bryce and Brot, ). The Fe-bound siderophores are subsequently transported into the cell to satisfy an Fe2+ requirement. Because Fe2+ transcriptionally controls expression of gene products that promote iron acquisition, Fe2+ was predicted to be a corepressor for a DNA-binding protein. Isolation of a mutant of Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) that constitutively expresses iron uptake proteins supported this hypothesis (Ernst et al., ). A mutation in Ferric Uptake Regulator (Fur) encoded by the fur gene was identified in E. coli mutants that exhibited constitutive expression of iron uptake genes (Hantke,,; Bagg and Neilands, ). Fur is a DNA-binding protein that recognizes specific DNA sequences, utilizes Fe2+ or Mn2+ as a corepressor, and blocks transcription of target genes (Bagg and Neilands,; De Lorenzo et al., ). Not surprisingly, the transcriptional control of entD, entF, entCEBA, and iucABCD is negatively regulated by Fur (De Lorenzo et al.,; Brickman et al.,; Stojiljkovic et al.,; Tsolis et al.,; Bjarnason et al.,; McHugh et al.,; Troxell et al., ).

The collective work supports a simple model for the molecular mechanism of Fur repression that consists of Fur binding to cis regulatory elements of a gene and preventing the binding of the RNA polymerase holoenzyme (RNAP) (Figure 1B) (De Lorenzo et al.,; Escolar et al.,,; Hantke,; Lee and Helmann,; Carpenter et al., ). As a transcriptional repressor, Fur-Fe2+ homodimer binds to the operator site of a target promoter (Ernst et al.,; Bagg and Neilands,,; Neilands,; Escolar et al.,, ). However, Fur can form a multimeric complex with DNA sequences extending beyond the operator site (Escolar et al.,; Baichoo and Helmann,; Lavrrar et al., ). Initial studies defined the Fur-binding site (the Fur box) as an ≈19 bp DNA sequence with dyad symmetry, GATAATGATAATCATTATC (Calderwood and Mekalanos,,; De Lorenzo et al.,; Stojiljkovic et al., ). Insertion of this sequence into an operator site in the promoter of a non-Fe2+ regulated gene results in derepression under Fe2+-limiting conditions (Calderwood and Mekalanos, ). In an elegant approach to define Fur regulated genes within bacteria, a high copy number plasmid containing randomly cloned DNA sequences from Gram positive and negative bacteria were transformed into an E. coli strain that harbored a single copy of a fhuF::lacZ reporter fusion (Hantke, ). Fur represses transcription of the fhuF gene, which encodes a protein involved in the acquisition of Fe3+ (Hantke,, ). If the cloned DNA fragment on the high copy number plasmid contains a Fur-binding site, then Fur proteins will be titrated away from the promoter of fhuF resulting in derepression of the fhuF::lacZ fusion, which can be qualitatively detected during growth on MacConkey agar plates or quantified by a β-galactosidase assay. This assay is called the Fur titration assay (FURTA) and has been used to study Fur regulation for nearly 20 years (Stojiljkovic et al.,; Tsolis et al.,; Baumler et al.,; Fassbinder et al.,; Osorio et al.,; Haraszthy et al.,; Jackson et al.,; Tanabe et al., ). In toto, these works solidified the role of Fur as a Fe2+-dependent transcriptional repressor. However, global gene expression studies have identified numerous genes that require Fur for expression (Foster and Hall,; D'Autreaux et al.,; Bjarnason et al.,; McHugh et al.,; Troxell et al., ).

Multifactorial roles of Fe2+-Fur regulation in bacteria

Fur is required for the expression of several proteins within the tricarboxylic acid cycle (TCA) and the Fe2+-dependent superoxide dismutase (SodB) (Hantke,; Gruer and Guest,; Dubrac and Touati,, ). The disruption of the TCA cycle within fur mutants may have a relevant role for the regulation of virulence since mutations within the TCA cycle alter virulence expression in Staphylococcus epidermidis and Vibrio cholera (Sadykov et al.,; Minato et al., ). In addition, disruption of the TCA cycle reduces S. Typhimurium virulence in mice (Tchawa Yimga et al.,; Bowden et al., ). The role of Fur in TCA cycle regulation is an example of how Fur regulation is multifactorial; fur mutants exhibit many phenotypes not just enhanced expression of siderophores. The molecular mechanism for the Fur's positive activation in the TCA cycle and SodB went unexplained until a landmark publication determined the importance of a highly conserved small untranslated RNA (sRNA) named ryhB in activation by Fur (Masse and Gottesman, ). ryhB is directly repressed by Fur (Vassinova and Kozyrev,; Masse and Gottesman, ) and base pairs with target mRNAs, such as sodB and the succinate dehydrogenase operon sdhCDAB, which results in degradation of the mRNAs thereby reducing expression of the gene products (Figure 2A). Deletion of ryhB in a Δfur results in restoration of expression of TCA proteins, SodB, and growth on succinate or fumarate minimal medium (Masse and Gottesman, ). Because regulation by ryhB requires the RNA chaperone protein, Hfq, deletion of hfq in Δfur also restores expression of many Fur activated genes (Masse and Gottesman,; Ellermeier and Slauch,; Troxell et al., ). ryhB homologs have a role in virulence, are Fur-repressed, and are encoded in the genomes of several Gram negative pathogens (i.e., Klebsiella pneumoniae, Shigella, Vibrio cholera, Yersinia pestis, Salmonella, Pseudomonas aeruginosa, Neisseria meningitidis, and Neisseria gonorrhoeae) (Wilderman et al.,; Davis et al.,; Mey et al.,; Oglesby et al.,; Mellin et al.,; Murphy and Payne,; Ellermeier and Slauch,; Ducey et al.,; Metruccio et al.,; Troxell et al.,; Deng et al.,; Huang et al.,; Kim and Kwon,; Leclerc et al., ). Indirect positive regulation by Fur through negative regulation of the negative regulator, ryhB, is the most studied molecular mechanism for Fe2+-dependent activation of gene expression; however, additional evidence demonstrates that Fur may regulate virulence through more complicated mechanisms.

For example, in S. Typhimurium, transcription of the virulence factor hilA requires Fe2+ through Fur-dependent regulation (Thompson et al.,; Ellermeier and Slauch,; Troxell et al., ). Recently, we demonstrated enhanced transcription of hns in Δfur and in a modified chromatin immunoprecipitation (ChIP) assay we determined that Fur bound the upstream regulatory region of hns in a metal-dependent manner (Troxell et al., ). H-NS is known to repress transcription of hilA (Olekhnovich and Kadner, ). H-NS is a protein associated with the bacterial nucleoid and is also known as OsmZ, BglY, and PilG (Defez and De Felice,; Spears et al.,; May et al., ). Deletion of fur and hns resulted in Fur-independent activation of hilA, which supports the indication that Fur regulation of hilA was indirect through H-NS (Troxell et al., ). Furthermore, Fur is not required for expression of Fur-activated genes when the repressor H-NS is absent (Nandal et al.,; Troxell et al., ) and Fur and H-NS appeared to recognize similar DNA sequences throughout the bacterial chromosome (Prajapat and Saini, ). In another example of the multifactorial role of Fur in bacteria, a recent study shows that Fur represses transcription of the vvhA gene, which encodes the major haemolysin of Vibrio vulnificus, yet haemolytic activity and VvhA protein level were reduced in Δfur (Lee et al., ). Two metal-dependent proteases are responsible for degradation of VvhA, VvpE, and VvpM and transcription of vvpE is under negative regulation by Fur. Through genetic and biochemical approaches, it was shown that VvpE and VvpM exhibited enhanced activity in Δfur resulting in reduction of the VvhA protein (Lee et al., ). Clearly, it can be appreciated from these two examples that the influence of Fur within the cell is global and typically involves multiple layers of regulation. Nevertheless, recent evidence indicates Fur may have a more direct role for activation of gene expression in bacteria (Figure 2).

Mechanisms of activation of gene expression via DNA binding by fur: location, location, location

Global gene expression studies have identified genes that require Fur for expression (Foster and Hall,; D'Autreaux et al.,; Bjarnason et al.,; McHugh et al.,; Troxell et al., ). Earlier work demonstrated a unique mechanism for Fur activation in N. meningitidis that involves Fur directly binding to cis regulatory elements upstream of a Fur-activated gene (Delany et al., ). Unlike Fur-repressed genes that possess a characteristic Fur-binding site overlapping the RNAP-binding site, Fur-activated genes [norB, pan1 (aniA), and nuoA] contain Fur boxes located ≈100 bp upstream of the transcriptional start site, while the Fur-repressed tbp contains a Fur box that overlaps with the RNAP-binding site. The Fur box and activation of norB, which encodes a protein responsible for protection against NO (Anjum et al., ), is conserved in N. gonorrhoeae (Isabella et al., ). Moreover, in Helicobacter pylori, Fur activates expression of oorB, which encodes a 2-oxoglutarate:acceptor oxidoreductase (Hughes et al., ), by directly binding to a cis regulatory elements located 130 bp upstream of the transcriptional start site (Gilbreath et al., ). The importance of OorB in virulence is demonstrated by the significant reduction in colonization of the chicken gut by a ΔoorB mutant strain of Campylobacter jejuni (Weerakoon et al., ). In V. cholera, Fur activates expression of the outer membrane porin, ompT, through binding a Fur box located 90 bp upstream of the transcriptional start site (Craig et al., ). In S. Typhimurium, transcription of the virulence factor hilD is activated by Fur through a Fur box located nearly 200 bp upstream of the transcriptional start site (Teixido et al., ). HilD is an AraC/XylS-type DNA-binding protein that regulates transcription of important virulence factors within S. Typhimurium and is required for infection (Ellermeier et al., ). Importantly, the sequence of the Fur box site for activated genes is virtually identical to the Fur box of repressed genes. Collectively, the molecular evidence suggests the location of the Fur box in proximity to the RNAP-binding site determines the ability of Fur to activate gene expression.

How does Fur activate gene expression? In vitro transcription experiments demonstrate that Fur can activate transcription of a target gene even though the Fur boxes are located ≈100 and 200 bp upstream of the transcriptional start site, respectively (Delany et al.,; Teixido et al., ). This example of Fur activation is rare, but may involve enhanced recruitment of RNAP to the promoter of target genes (“RNAP recruitment” activation model, Figure 2B). Surprisingly, addition of the Fur protein to the in vitro transcription assay stimulated the production of hilD mRNA, which suggests improved recruitment of RNAP to the promoter of hilD even though the Fur box is nearly 200 bp upstream of the transcriptional start site (Teixido et al., ). While deletion of fur reduces transcription of hilD (Teixido et al., ) overexpression of Fur results in little increased activation of the hilD promoter contrary to overexpression of a direct activator HilC, which increases hilD's promoter activity by ≈5-fold (Ellermeier and Slauch, ). These results indicate the role of Fur in direct transcriptional activation of a target gene is complex.

Transcriptional activators that bind upstream of the RNAP-binding site have been shown to interact with the C-terminal domain of the α subunit (α-CTD) of RNAP, which promotes transcription of the target gene (Ishihama,; Busby and Ebright,; Ebright and Busby,; Murakami et al.,; Hochschild and Dove, ). Contact between activators and α-CTD is inhibited when the upstream activator binding site is ≥100 bp upstream of the transcriptional start site (Murakami et al., ). Thus, transcription factor binding sites located further than 100 bp upstream of the transcriptional start site are unlikely to interact physically with the α-CTD of RNAP. However, oligomerization of the Fur protein at Fur boxes is known to occur (De Lorenzo et al.,; Tardat and Touati,; Escolar et al.,; Nandal et al.,; Teixido et al., ), which suggests Fur proteins may extend to interact with other proteins nearby. Whether Fur contacts the RNAP is not known, but emerging in vivo evidence indicates there is another plausible molecular mechanism for Fur-dependent activation through binding DNA at a distal regulatory site.

Roles of Fur and H-NS in the regulation of FtnA

Fe2+ activates expression of the Fe-storage gene ftnA in a Fur-dependent manner (Masse and Gottesman,; Velayudhan et al., ). Overexpression of ryhB results in the down regulation of many Fe-cofactored proteins (i.e., SodB) and increases the intracellular Fe2+ concentration resulting in enhanced Fur activation (Masse et al.,; Jacques et al., ). This is known as the “iron-sparing” response (Gaballa et al., ). Masse et al. theorized that Fur may negatively regulate a negative regulator of ftnA, which would manifest as a Fur activation. Evidence to support this theory was demonstrated by work from Simon C. Andrews' lab, which showed that Fur binds to a distal regulator site upstream of the RNAP-binding site in the promoter of ftnA to physically remove the histone-like protein, H-NS, which mediates repression of ftnA (Nandal et al., ). Unlike the activation of norB and hilD, Fur was not required for transcription of ftnA using in vitro transcription assays (Nandal et al., ). H-NS repressed transcription of ftnA and Fur was only required to relieve this repression. The role of Fur as an antirepressor in the activation of ftnA is supported with in vivo evidence: (1) fur is not required for ftnA expression in the absence of hns; and (2) ftnA expression is not reduced by Fe2+-chelation in Δhns (Nandal et al., ). Fur activation of gene expression by this mode represents a 3rd type of activation, the “antirepressor” activation model (Figure 2C). In vivo evidence supports the antirepressor model as a major mechanism for Fur-dependent activation of gene expression. Evidence for the antirepressor model is evident in N. gonorrhoeae because the Fur-binding site upstream of norB is not required for activation of expression when the norB repressor, ArsR, is deleted (Isabella et al., ). Thus, Fur antirepressor activity is an emerging model of Fur activation through DNA binding.

Fur controls defenses against ROS

During bacterial infection the host responds to non-self molecules and initiates a potent antimicrobial response. However, bacterial pathogens are well-adapted to defending against the host antimicrobial response. In many bacterial pathogens the defense against ROS requires the Fur protein. Enzymatic defense against ROS occurs by the rapid enzymatic dismutation of superoxide (O2−) by superoxide dismutases (SODs) and detoxification of H2O2 by hydroperoxidases [i.e., the heme containing peroxidase/catalase (HPI), and the heme containing catalase (HPII)]. Unlike most pathogenic bacteria, S. Typhimurium contains 6 genes whose gene products are devoted toward degradation of H2O2. HPI (encoded by katG), HPII (encoded by katE), a Mn-dependent catalase (encoded by katN), an NADH-dependent alkyl peroxidase system (encoded by ahpCF), and two thiol specific peroxidases (encoded by tsaA and tpx). HPII and KatN are under positive regulation by the alternative σ factor RpoS, whereas HPI is induced by the redox sensing regulator OxyR during hydrogen peroxide stress (Tartaglia et al.,; Ivanova et al.,; Robbe-Saule et al.,; Vazquez-Torres, ). In addition, OxyR activates expression of ahpC (Storz et al.,; Tartaglia et al., ) and also fur (Zheng et al.,; Varghese et al., ). Regulation of tsaA appears Fur-independent (Delany et al., ) and there is a lack of evidence for whether Fe2+ and perhaps Fur regulate tpx. Deletion of any single gene or in combinations does not influence virulence; only the combined deletion of 5 out of the 6 genes results in reduced virulence signifying the importance of redundant H2O2 scavengers to virulence (Hebrard et al.,; Horst et al., ). As evident from studies in other bacterial pathogens, there are profound redundancies that contribute to resistance to H2O2 and virulence in vivo (Cosgrove et al.,; Lindgren et al.,; Soler-Garcia and Jerse, ). Because SODs and H2O2-degrading enzymes require certain metals as cofactors for enzymatic function and because Fur is a redox sensing protein (Fleischhacker and Kiley, ), it is not surprising that Fur is involved in the regulation of defenses against ROS.

SODs and HPI/HPII require the appropriate cofactors; Fe2+ is required for FeSOD (SodB) and Mn2+ for MnSOD (SodA) whereas heme is required for HPI and HPII function (Keele et al.,; Yost and Fridovich,; Hassan and Fridovich,; Claiborne and Fridovich,; Claiborne et al., ). Fur directly represses transcription of the gene encoding the MnSOD (sodA) and indirectly activates expression of the gene encoding the FeSOD (sodB; Niederhoffer et al.,; Tardat and Touati,; Beaumont and Hassan, ). This indirect control of sodB requires the RNA chaperone Hfq or ryhB (Masse and Gottesman,; Ellermeier and Slauch,; Troxell et al., ). In addition, Fur controls HPI/HPII activity in a complex manner that may depend on the ability of Fur to regulate biosynthesis of the heme cofactor (Hamza et al.,; Benov and Sequeira,; Hoerter et al.,; Mey et al.,; Gaballa et al., ) (R. Saah and H. M. Hassan, unpublished data). Surprisingly, despite the enhanced transcription of sodA in Δfur, a corresponding increase in MnSOD activity was not observed due to the increased [Fe2+] in the mutant. Indeed, increase in MnSOD activity in Δfur was only discernible upon supplementation of the growth medium with excess [Mn2+] in order to outcompete the available Fe2+ for the active site of MnSOD (Hassan and Schrum,; Schrum and Hassan,; Troxell et al., ). Thus, with respect to O−2 defense Δfur behaves phenotypically like ΔsodAΔsodB under Fe2+ replete conditions. The Fur regulation of Mn2+ transport is well-documented (Patzer and Hantke,; Kehres et al.,; Guedon et al.,; Ikeda et al.,; Runyen-Janecky et al.,; Perry et al., ). Furthermore, because katN encodes a Mn-containing catalase and is activated by RpoS (Robbe-Saule et al., ) and repressed by H-NS (Beraud et al., ), it is likely that Fur is involved in katN expression in S. Typhimurium. Thus, the modulation of the intracellular Mn2+ concentration will undoubtedly influence protection against ROS and likely virulence. In support of this, numerous studies have demonstrated the importance of Mn2+ in the regulation of virulence and infectivity (Boyer et al.,; Corbin et al.,; Anderson et al.,; Ouyang et al.,; Ogunniyi et al.,; Wu et al.,; Champion et al.,; Kehl-Fie et al.,; Damo et al.,; Troxell et al., ). Likewise, additional members of the Fur family of metal-dependent transcription factors either bind Mn2+ directly and/or regulate Mn2+ transport.

Fe2+ sequestration by the host

Because Fur requires Fe2+ as a corepressor the availability of this metal controls Fur activity. Moreover, the Fe2+-Fur complex is inactivated by ROS and reactive nitrogen species (RNS) (D'Autreaux et al.,; Varghese et al., ), both of which are generated by the host during infection. Humans and other higher eukaryotes produce numerous proteins that sequester free Fe2+ and heme to deprive the pathogens of iron and meanwhile prevent the toxic formation of ROS. A potent antimicrobial response, including ROS production, produced by innate cells of the host's immune system is activated in response to detection of pathogen-associated molecular patterns (PAMPs) during bacterial infection. Innate cell activation by PAMPs initiates the synthesis of large amounts of Fe2+ sequestering proteins to limit the available Fe pool for the pathogen, known as “nutritional immunity” (Kehl-Fie and Skaar,; Hood and Skaar, ) and activates signaling pathways that causes the host to reduce dietary absorption of Fe that is known as “the anemia of inflammation.” In addition, the host responds to infection by increasing the body temperature (the febrile response) as a means to inhibit bacterial growth. The antimicrobial host factors produced during activation of nutritional immunity can be inhibited by the addition of Fe (Weinberg, ). Furthermore, the febrile response to bacterial pathogens is antimicrobial, in part, due to the reduced ability of bacteria to acquire Fe2+ at febrile temperatures (Kluger and Rothenburg, ).

Anemia of inflammation by the host in response to infection has been known for more than 60 years (Cartwright et al.,; Greenberg et al.,; Wintrobe et al., ) and the host protein, hepcidin, controls this response (Nicolas et al.,; Nemeth et al.,,). In addition, hepcidin is a host factor that strongly reduces the absorption of dietary Fe (Shayeghi et al.,; Drakesmith and Prentice,; Prentice et al., ). Because Fe2+ is required for cellular function within nearly all cells, limiting the availability of Fe2+ starves pathogens for Fe2+ and weakens the pathogens' ability to combat antimicrobial responses by the host. Not surprisingly, there is fierce competition for accessibility of Fe2+ during infection. Phagocytosis of the intracellular pathogen S. Typhimurium by macrophages enhances expression of the Fe2+ export protein ferroportin, which limits the available Fe2+ during intracellular residence of S. Typhimurium (Nairz et al., ). Expression of ferroportin correlates directly with reduced bacterial burden of several intracellular pathogens (Paradkar et al., ). Thus, the host responds to infection by sequestering Fe2+ from the local environment of pathogens, limits the absorption of dietary Fe resulting in a very Fe2+ limiting host environment, and restricts available Fe2+ within the phagosome.

An important host factor that controls bacterial infection is the natural resistance-associated macrophage protein 1 (NRAMP1, also known as SLC11A1) and several research groups determined the contribution of the SLC11A1 locus to severity of infection within animal models (Plant and Glynn,; Bradley,; Skamene et al.,; Brown et al., ). S. Typhimurium lacking fur are avirulent within mice with a functional NRAMP1, whereas the isogenic parent is fully virulent. Mice lacking a functional NRAMP1 are partially resistant to infection with Δfur demonstrating that Fur function is important for virulence, in part, independent of the host NRAMP1 function (Troxell et al., ). Evidence indicates that Fur is functional within an unstimulated macrophage cell-line expressing either a functional or mutated NRAMP1 (Taylor et al., ). The NRAMP1 protein is a highly conserved transporter of divalent cations and is expressed within phagocytic cells (Cellier et al.,; Canonne-Hergaux et al.,; Cellier, ); NRAMP1 functions as a transporter of manganese (Mn2+), Fe2+, or cobalt (Co) and is important for acidification of the phagosome (Hackam et al.,; Jabado et al.,; Forbes and Gros, ). Furthermore, NRAMP1 promotes additional host factors of the antimicrobial response including production of nitric oxide (NO) (Fritsche et al.,,; Nairz et al., ) and production of lipocalin-2 (also called siderocalin), which binds to bacterial siderophores thereby sequestering bacterial Fe2+ acquisition proteins (Fritsche et al., ). However, bacteria have evolved a counter defense mechanism by producing salmochelins, which are structurally distinct from enterochelin and therefore not susceptible to binding by lipocalin-2 (Smith, ). RNS and NO perturb Fur-Fe2+ function within pathogens (Mukhopadhyay et al.,; Richardson et al.,; Bourret et al., ). NO is a crucial factor in the antimicrobial response and its production is regulated by Fe2+ (Weiss et al.,; Melillo et al.,; Dlaska and Weiss, ). Consequently, the inability to generate NO increases the Fe2+ content within macrophages, splenic cells, and hepatocytes thereby increasing disease severity in animal models of infection (Nairz et al., ). This signifies the importance of NRAMP1 in the ability to sequester Fe2+ from pathogens and in general antimicrobial response.

Control of virulence by the fur family of transcriptional regulators

The Fur protein contributes to virulence in animal models for numerous bacterial pathogens (Table 1). Although the precise mechanism for the observed attenuation of fur mutants is not clear, evidence indicates that a reduction in the activity of enzymes required for protection against ROS may be involved. Furthermore, virulence factors within the fur mutants exhibit altered expression or activity, which may additionally contribute to a decrease in virulence. Because Fur also controls expression or activity of enzymes within the TCA cycle, fur mutants are defective in the utilization of several carbon sources (i.e., succinate, etc.), which may contribute to the inability of fur mutants to cause disease within animal hosts.

There are additional transcription factors within the Fur family that require alternative metals to control gene regulation and virulence. First discovered by work in B. subtilis within the lab of John Helmann (Bsat et al.,; Mongkolsuk and Helmann, ), PerR is widespread in other bacteria and contributes to virulence within pathogens (Van Vliet et al.,; Horsburgh et al.,; Rea et al.,,; Gryllos et al., ). The DNA-binding activity of PerR is sensitive to relevant concentrations of H2O2 and upon metal-dependent oxidation results in derepression of target genes (Lee and Helmann, ). PerR homodimers are detected as two forms, one which contains two ions of Zn2+/Fe2+ per monomer and one which contains two ions of Zn2+/Mn2+ per monomer. Only the Zn/Fe form is sensitive to H2O2-induced derepression and, as expected, PerR regulates genes whose protein products detoxify H2O2 (Herbig and Helmann,; Lee and Helmann, ). Thus, the H2O2-sensing of PerR is directly influenced by the Mn2+:Fe2+ ratio within the cell. Maintenance of the Mn2+:Fe2+ ratio is an important aspect within bacterial pathogens (Veyrier et al., ).

Zinc (Zn2+) uptake regulator (Zur) is a Fur family regulator that responds to Zn2+ and was discovered by two groups working with E. coli and Bacillus subtilis (B. subtilis) (Gaballa and Helmann,; Patzer and Hantke, ). As expected for a Fur homolog, Zur represses transcription of Zn2+ uptake when bound to the corepressor Zn2+ (Patzer and Hantke,; Gaballa and Helmann, ). Because ribosomal proteins utilize Zn2+ for activity Zur also represses transcription of genes involved in mobilization of Zn2+ by ribosomal protein paralogs, which may allow for protein synthesis under conditions of Zn2+ limitation known as the “failsafe” model (Maciag et al.,; Natori et al.,; Gabriel and Helmann, ). The Zur protein or Zn2+ uptake systems have an important role for bacterial pathogens, which demonstrate the importance of Zn2+ acquisition during infection (Campoy et al.,; Ammendola et al.,; Sabri et al.,; Smith et al.,; Desrosiers et al.,; Pesciaroli et al.,; Corbett et al.,; Dowd et al.,; Gielda and Dirita, ). The ability to acquire Zn2+ by bacterial pathogens is likely a broad requirement among bacterial pathogens during infection. More recently, a Fur-homolog was characterized as a Mn2+-dependent DNA-binding protein (Mur). This regulator, originally isolated from Rhizobium leguminosarum (Diaz-Mireles et al.,,; Bellini and Hemmings, ), utilizes Mn2+ as a corepressor. In contrast to Fur and Zur, the role of Mur in bacterial pathogenesis is less understood. However, genes regulated by Mur are important for virulence in the pathogen Brucella abortus (Anderson et al.,; Menscher et al., ), which indicates Mur function may be important to virulence.

Conclusions

The Fur family of transcriptional regulators control virulence, defense against ROS, and transport of Fe2+, Zn2+, and Mn2+. Because of the anemia of inflammation and nutritional immunity exerted by the host during infection, metals are in low abundance in response to infection. In this metal-poor environment, the demetaleted Fur would allow for efficient acquisition of iron and enhances the fitness of the pathogen; however, deletion of fur most often results in partial or complete attenuation within animal models of infection. Notably, deletion of fur results in reduced expression of active enzymes responsible for defense against ROS, reduced expression of key metabolic pathways, and reduced expression of important virulence factors. This signifies that Fur's critical contribution to virulence may not be due to its classical role as a transcriptional repressor of metal acquisition (Figure 1), but to its complex role as a transcriptional activator of virulence (Figure 2).

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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    Anjum M. F. Stevanin T. M. Read R. C. Moir J. W. (2002 ). Nitric oxide metabolism in Neisseria meningitidis. J. Bacteriol. 184, 2987–2993

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    Bagg A. Neilands J. B. (1985 ). Mapping of a mutation affecting regulation of iron uptake systems in Escherichia coli K-12. J. Bacteriol. 161, 450–453

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    Bagg A. Neilands J. B. (1987a ). Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli. Biochemistry 26, 5471–5477

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    Bagg A. Neilands J. B. (1987b ). Molecular mechanism of regulation of siderophore-mediated iron assimilation. Microbiol. Rev. 51, 509–518

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    Baichoo N. Helmann J. D. (2002 ). Recognition of DNA by Fur: a reinterpretation of the Fur box consensus sequence. J. Bacteriol. 184, 5826–5832

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    Baumler A. J. Tsolis R. M. Van Der Velden A. W. Stojiljkovic I. Anic S. Heffron F. (1996 ). Identification of a new iron regulated locus of Salmonella typhi. Gene 183, 207–213

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    Beaumont M. D. Hassan H. M. (1993 ). Characterization of regulatory mutations causing anaerobic derepression of the sodA gene in Escherichia coli K12: cooperation between cis- and trans-acting regulatory loci. J. Gen. Microbiol. 139, 2677–2684

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    Bellini P. Hemmings A. M. (2006 ). In vitro characterization of a bacterial manganese uptake regulator of the fur superfamily. Biochemistry 45, 2686–2698

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    Benov L. Sequeira F. (2003 ). Escherichia coli deltafur mutant displays low HPII catalase activity in stationary phase. Redox Rep. 8, 379–383

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    Beraud M. Kolb A. Monteil V. D'Alayer J. Norel F. (2010 ). A proteomic analysis reveals differential regulation of the sigma(S)-dependent yciGFE(katN) locus by YncC and H-NS in Salmonella and Escherichia coli K-12. Mol. Cell. Proteomics 9, 2601–2616

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  16. 16.
    An incomplete TCA cycle increases survival of Salmonella Typhimurium during infection of resting and activated murine macrophages. · 2010
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    Boyer E. Bergevin I. Malo D. Gros P. Cellier M. F. (2002 ). Acquisition of Mn(II) in addition to Fe(II) is required for full virulence of Salmonella enterica serovar Typhimurium. Infect. Immun. 70, 6032–6042

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    Bradley D. J. (1977 ). Regulation of Leishmania populations within the host. II. genetic control of acute susceptibility of mice to Leishmania donovani infection. Clin. Exp. Immunol. 30, 130–140

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    Brot N. Goodwin J. (1968 ). Regulation of 2,3-dihydroxybenzoylserine synthetase by iron. J. Biol. Chem. 243, 510–513

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    Brown D. E. Libby S. J. Moreland S. M. McCoy M. W. Brabb T. Stepanek A. (2013 ). Salmonella enterica Causes more severe inflammatory disease in C57/BL6 Nramp1G169 Mice Than Sv129S6 Mice. Vet. Pathol. 50, 867–876

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    Bryce G. F. Brot N. (1971 ). Iron transport in Escherichia coli and its relation to the repression of 2,3-dihydroxy-N-benzoyl-L-serine synthetase. Arch. Biochem. Biophys. 142, 399–406

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    Bsat N. Herbig A. Casillas-Martinez L. Setlow P. Helmann J. D. (1998 ). Bacillus subtilis contains multiple Fur homologues: identification of the iron uptake (Fur) and peroxide regulon (PerR) repressors. Mol. Microbiol. 29, 189–198

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    Bury-Mone S. Thiberge J. M. Contreras M. Maitournam A. Labigne A. De Reuse H. (2004 ). Responsiveness to acidity via metal ion regulators mediates virulence in the gastric pathogen Helicobacter pylori. Mol. Microbiol. 53, 623–638

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  29. 29.

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    Cartwright G. E. Lauritsen M. A. Jones P. J. Merrill I. M. Wintrobe M. M. (1946 ). The anemia of infection. i. hypoferremia, hypercupremia, and alterations in porphyrin metabolism in patients. J. Clin. Invest. 25, 65–80

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    Champion O. L. Karlyshev A. Cooper I. A. Ford D. C. Wren B. W. Duffield M. (2011 ). Yersinia pseudotuberculosis mntH functions in intracellular manganese accumulation, which is essential for virulence and survival in cells expressing functional Nramp1. Microbiology 157, 1115–1122

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    Claiborne A. Fridovich I. (1979 ). Purification of the o-dianisidine peroxidase from Escherichia coli B. Physicochemical characterization and analysis of its dual catalatic and peroxidatic activities. J. Biol. Chem. 254, 4245–4252

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    Corbett D. Wang J. Schuler S. Lopez-Castejon G. Glenn S. Brough D. (2012 ). Two zinc uptake systems contribute to the full virulence of Listeria monocytogenes during growth in vitro and in vivo. Infect. Immun. 80, 14–21

    Not in our library10.1128/iai.05904-11

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    Corbin B. D. Seeley E. H. Raab A. Feldmann J. Miller M. R. Torres V. J. (2008 ). Metal chelation and inhibition of bacterial growth in tissue abscesses. Science 319, 962–965

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    Cosgrove K. Coutts G. Jonsson I. M. Tarkowski A. Kokai-Kun J. F. Mond J. J. (2007 ). Catalase (KatA) and alkyl hydroperoxide reductase (AhpC) have compensatory roles in peroxide stress resistance and are required for survival, persistence, and nasal colonization in Staphylococcus aureus. J. Bacteriol. 189, 1025–1035

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  40. 40.

    Craig S. A. Carpenter C. D. Mey A. R. Wyckoff E. E. Payne S. M. (2011 ). Positive regulation of the Vibrio cholerae porin OmpT by iron and fur. J. Bacteriol. 193, 6505–6511

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    Curtiss R. 3rd. Wanda S. Y. Gunn B. M. Zhang X. Tinge S. A. Ananthnarayan V. (2009 ). Salmonella enterica serovar typhimurium strains with regulated delayed attenuation in vivo. Infect. Immun. 77, 1071–1082

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  42. 42.

    Damo S. M. Kehl-Fie T. E. Sugitani N. Holt M. E. Rathi S. Murphy W. J. (2013 ). Molecular basis for manganese sequestration by calprotectin and roles in the innate immune response to invading bacterial pathogens. Proc. Natl. Acad. Sci. U.S.A. 110, 3841–3846

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  43. 43.

    D'Autreaux B. Touati D. Bersch B. Latour J. M. Michaud-Soret I. (2002 ). Direct inhibition by nitric oxide of the transcriptional ferric uptake regulation protein via nitrosylation of the iron. Proc. Natl. Acad. Sci. U.S.A. 99, 16619–16624

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    Davis B. M. Quinones M. Pratt J. Ding Y. Waldor M. K. (2005 ). Characterization of the small untranslated RNA RyhB and its regulon in Vibrio cholerae. J. Bacteriol. 187, 4005–4014

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  45. 45.

    De Lorenzo V. Wee S. Herrero M. Neilands J. B. (1987 ). Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor. J. Bacteriol. 169, 2624–2630

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  46. 46.

    Defez R. De Felice M. (1981 ). Cryptic operon for beta-glucoside metabolism in Escherichia coli K12: genetic evidence for a regulatory protein. Genetics 97, 11–25

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    Delany I. Pacheco A. B. Spohn G. Rappuoli R. Scarlato V. (2001 ). Iron-dependent transcription of the frpB gene of Helicobacter pylori is controlled by the Fur repressor protein. J. Bacteriol. 183, 4932–4937

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  48. 48.

    Delany I. Rappuoli R. Scarlato V. (2004 ). Fur functions as an activator and as a repressor of putative virulence genes in Neisseria meningitidis. Mol. Microbiol. 52, 1081–1090

    Not in our library10.1111/j.1365-2958.2004.04030.x

  49. 49.

    Deng Z. Meng X. Su S. Liu Z. Ji X. Zhang Y. (2012 ). Two sRNA RyhB homologs from Yersinia pestis biovar microtus expressed in vivo have differential Hfq-dependent stability. Res. Microbiol. 163, 413–418

    Not in our library10.1016/j.resmic.2012.05.006

  50. 50.

    Desrosiers D. C. Bearden S. W. Mier I. Jr. Abney J. Paulley J. T. Fetherston J. D. (2010 ). Znu is the predominant zinc importer in Yersinia pestis during in vitro growth but is not essential for virulence. Infect. Immun. 78, 5163–5177

    Not in our library10.1128/iai.00732-10

  51. 51.

    Diaz-Mireles E. Wexler M. Sawers G. Bellini D. Todd J. D. Johnston A. W. (2004 ). The Fur-like protein Mur of Rhizobium leguminosarum is a Mn(2+)-responsive transcriptional regulator. Microbiology 150, 1447–1456

    Not in our library10.1099/mic.0.26961-0

  52. 52.

    Diaz-Mireles E. Wexler M. Todd J. D. Bellini D. Johnston A. W. Sawers R. G. (2005 ). The manganese-responsive repressor Mur of Rhizobium leguminosarum is a member of the Fur-superfamily that recognizes an unusual operator sequence. Microbiology 151, 4071–4078

    Not in our library10.1099/mic.0.28342-0

  53. 53.

    Dlaska M. Weiss G. (1999 ). Central role of transcription factor NF-IL6 for cytokine and iron-mediated regulation of murine inducible nitric oxide synthase expression. J. Immunol. 162, 6171–6177

    Not in our library

  54. 54.

    Dowd G. C. Casey P. G. Begley M. Hill C. Gahan C. G. (2012 ). Investigation of the role of ZurR in the physiology and pathogenesis of Listeria monocytogenes. FEMS Microbiol. Lett. 327, 118–125

    Not in our library10.1111/j.1574-6968.2011.02472.x

  55. 55.

    Drakesmith H. Prentice A. M. (2012 ). Hepcidin and the iron-infection axis. Science 338, 768–772

    Not in our library10.1126/science.1224577

  56. 56.

    Dubrac S. Touati D. (2000 ). Fur positive regulation of iron superoxide dismutase in Escherichia coli: functional analysis of the sodB promoter. J. Bacteriol. 182, 3802–3808

    Not in our library10.1128/jb.182.13.3802-3808.2000

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    Dubrac S. Touati D. (2002 ). Fur-mediated transcriptional and post-transcriptional regulation of FeSOD expression in Escherichia coli. Microbiology 148, 147–156

    Not in our library10.1099/00221287-148-1-147

  58. 58.

    Ducey T. F. Jackson L. Orvis J. Dyer D. W. (2009 ). Transcript analysis of nrrF, a Fur repressed sRNA of Neisseria gonorrhoeae. Microb. Pathog. 46, 166–170

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    Ebanks R. O. Goguen M. Knickle L. Dacanay A. Leslie A. Ross N. W. (2013 ). Analysis of a ferric uptake regulator (Fur) knockout mutant in Aeromonas salmonicida subsp. salmonicida. Vet. Microbiol. 162, 831–841

    Not in our library10.1016/j.vetmic.2012.10.038

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    Ebright R. H. Busby S. (1995 ). The Escherichia coli RNA polymerase alpha subunit: structure and function. Curr. Opin. Genet. Dev. 5, 197–203

    Not in our library10.1016/0959-437x(95)80008-5

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    Ellermeier C. D. Ellermeier J. R. Slauch J. M. (2005 ). HilD, HilC and RtsA constitute a feed forward loop that controls expression of the SPI1 type three secretion system regulator hilA in Salmonella enterica serovar Typhimurium. Mol. Microbiol. 57, 691–705

    Not in our library10.1111/j.1365-2958.2005.04737.x

  62. 62.

    Ellermeier J. R. Slauch J. M. (2008 ). Fur regulates expression of the Salmonella pathogenicity island 1 type III secretion system through HilD. J. Bacteriol. 190, 476–486

    Not in our library10.1128/jb.00926-07

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    Ernst J. F. Bennett R. L. Rothfield L. I. (1978 ). Constitutive expression of the iron-enterochelin and ferrichrome uptake systems in a mutant strain of Salmonella typhimurium. J. Bacteriol. 135, 928–934

    Not in our library10.1128/jb.135.3.928-934.1978

  64. 64.

    Escolar L. De Lorenzo V. Perez-Martin J. (1997 ). Metalloregulation in vitro of the aerobactin promoter of Escherichia coli by the Fur (ferric uptake regulation) protein. Mol. Microbiol. 26, 799–808

    Not in our library10.1046/j.1365-2958.1997.6211987.x

  65. 65.

    Escolar L. Perez-Martin J. De Lorenzo V. (1998 ). Coordinated repression in vitro of the divergent fepA-fes promoters of Escherichia coli by the iron uptake regulation (Fur) protein. J. Bacteriol. 180, 2579–2582

    Not in our library10.1128/jb.180.9.2579-2582.1998

  66. 66.

    Escolar L. Perez-Martin J. De Lorenzo V. (1999 ). Opening the iron box: transcriptional metalloregulation by the Fur protein. J. Bacteriol. 181, 6223–6229

    Not in our library10.1128/jb.181.20.6223-6229.1999

  67. 67.

    Escolar L. Perez-Martin J. De Lorenzo V. (2000 ). Evidence of an unusually long operator for the fur repressor in the aerobactin promoter of Escherichia coli. J. Biol. Chem. 275, 24709–24714

    Not in our library10.1074/jbc.m002839200

  68. 68.

    Fassbinder F. Van Vliet A. H. Gimmel V. Kusters J. G. Kist M. Bereswill S. (2000 ). Identification of iron-regulated genes of Helicobacter pylori by a modified fur titration assay (FURTA-Hp). FEMS Microbiol. Lett. 184, 225–229

    Not in our library10.1111/j.1574-6968.2000.tb09018.x

  69. 69.

    Fenton H. (1894 ). Oxidation of tartaric acid in presence of iron. J. Chem. Soc. Trans. 65, 899–911

    Not in our library10.1039/ct8946500899

  70. 70.

    Fleischhacker A. S. Kiley P. J. (2011 ). Iron-containing transcription factors and their roles as sensors. Curr. Opin. Chem. Biol. 15, 335–341

    Not in our library10.1016/j.cbpa.2011.01.006

  71. 71.

    Forbes J. R. Gros P. (2003 ). Iron, manganese, and cobalt transport by Nramp1 (Slc11a1) and Nramp2 (Slc11a2) expressed at the plasma membrane. Blood 102, 1884–1892

    Not in our library10.1182/blood-2003-02-0425

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    Foster J. W. Hall H. K. (1992 ). Effect of Salmonella typhimurium ferric uptake regulator (fur) mutations on iron- and pH-regulated protein synthesis. J. Bacteriol. 174, 4317–4323

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    Fritsche G. Dlaska M. Barton H. Theurl I. Garimorth K. Weiss G. (2003 ). Nramp1 functionality increases inducible nitric oxide synthase transcription via stimulation of IFN regulatory factor 1 expression. J. Immunol. 171, 1994–1998

    Not in our library10.4049/jimmunol.171.4.1994

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    Fritsche G. Nairz M. Libby S. J. Fang F. C. Weiss G. (2012 ). Slc11a1 (Nramp1) impairs growth of Salmonella enterica serovar typhimurium in macrophages via stimulation of lipocalin-2 expression. J. Leukoc. Biol. 92, 353–359

    Not in our library10.1189/jlb.1111554

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    Fritsche G. Nairz M. Werner E. R. Barton H. C. Weiss G. (2008 ). Nramp1-functionality increases iNOS expression via repression of IL-10 formation. Eur. J. Immunol. 38, 3060–3067

    Not in our library10.1002/eji.200838449

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    Gaballa A. Helmann J. D. (1998 ). Identification of a zinc-specific metalloregulatory protein, Zur, controlling zinc transport operons in Bacillus subtilis. J. Bacteriol. 180, 5815–5821

    Not in our library10.1128/jb.180.22.5815-5821.1998

  77. 77.

    Gaballa A. Helmann J. D. (2002 ). A peroxide-induced zinc uptake system plays an important role in protection against oxidative stress in Bacillus subtilis. Mol. Microbiol. 45, 997–1005

    Not in our library10.1046/j.1365-2958.2002.03068.x

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    Gaballa A. Antelmann H. Aguilar C. Khakh S. K. Song K. B. Smaldone G. T. (2008 ). The Bacillus subtilis iron-sparing response is mediated by a Fur-regulated small RNA and three small, basic proteins. Proc. Natl. Acad. Sci. U.S.A. 105, 11927–11932

    Not in our library10.1073/pnas.0711752105

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    Gabriel S. E. Helmann J. D. (2009 ). Contributions of Zur-controlled ribosomal proteins to growth under zinc starvation conditions. J. Bacteriol. 191, 6116–6122

    Not in our library10.1128/jb.00802-09

  80. 80.

    Gancz H. Censini S. Merrell D. S. (2006 ). Iron and pH homeostasis intersect at the level of Fur regulation in the gastric pathogen Helicobacter pylori. Infect. Immun. 74, 602–614

    Not in our library10.1128/iai.74.1.602-614.2006

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    Gehring A. M. Mori I. Walsh C. T. (1998 ). Reconstitution and characterization of the Escherichia coli enterobactin synthetase from EntB, EntE, and EntF. Biochemistry 37, 2648–2659

    Not in our library10.1021/bi9726584

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    Gielda L. M. Dirita V. J. (2012 ). Zinc competition among the intestinal microbiota. MBio 3, e00171-12

    Not in our library10.1128/mbio.00171-12

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    Gilbreath J. J. West A. L. Pich O. Q. Carpenter B. M. Michel S. Merrell D. S. (2012 ). Fur activates expression of the 2-oxoglutarate oxidoreductase genes (oorDABC) in Helicobacter pylori. J. Bacteriol. 194, 6490–6497

    Not in our library10.1128/jb.01226-12

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    Greenberg G. R. Ashenbrucker H. Lauritsen M. Worth W. Humphreys S. R. Wintrobe M. M. (1947 ). The anemia of infection. V. fate of injected radioactive iron in the presence of inflammation. J. Clin. Invest. 26, 121–125

    Not in our library10.1172/jci101784

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    Gruer M. J. Guest J. R. (1994 ). Two genetically-distinct and differentially-regulated aconitases (AcnA and AcnB) in Escherichia coli. Microbiology 140(Pt 10), 2531–2541

    Not in our library10.1099/00221287-140-10-2531

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    Gryllos I. Grifantini R. Colaprico A. Cary M. E. Hakansson A. Carey D. W. (2008 ). PerR confers phagocytic killing resistance and allows pharyngeal colonization by group A Streptococcus. PLoS Pathog. 4:e1000145

    Not in our library10.1371/journal.ppat.1000145

  87. 87.

    Guedon E. Moore C. M. Que Q. Wang T. Ye R. W. Helmann J. D. (2003 ). The global transcriptional response of Bacillus subtilis to manganese involves the MntR, Fur, TnrA and sigmaB regulons. Mol. Microbiol. 49, 1477–1491

    Not in our library10.1046/j.1365-2958.2003.03648.x

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    Hackam D. J. Rotstein O. D. Zhang W. Gruenheid S. Gros P. Grinstein S. (1998 ). Host resistance to intracellular infection: mutation of natural resistance-associated macrophage protein 1 (Nramp1) impairs phagosomal acidification. J. Exp. Med. 188, 351–364

    Not in our library10.1084/jem.188.2.351

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    Hamza I. Qi Z. King N. D. O'Brian M. R. (2000 ). Fur-independent regulation of iron metabolism by Irr in Bradyrhizobium japonicum. Microbiology 146(Pt 3), 669–676

    Not in our library10.1099/00221287-146-3-669

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    Hantke K. (1981 ). Regulation of ferric iron transport in Escherichia coli K12: isolation of a constitutive mutant. Mol. Gen. Genet. 182, 288–292

    Not in our library10.1007/bf00269672

  91. 91.

    Hantke K. (1983 ). Identification of an iron uptake system specific for coprogen and rhodotorulic acid in Escherichia coli K12. Mol. Gen. Genet. 191, 301–306

    Not in our library10.1007/bf00334830

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    Hantke K. (1984 ). Cloning of the repressor protein gene of iron-regulated systems in Escherichia coli K12. Mol. Gen. Genet. 197, 337–341

    Not in our library10.1007/bf00330982

  93. 93.

    Hantke K. (1987 ). Selection procedure for deregulated iron transport mutants (fur) in Escherichia coli K 12: fur not only affects iron metabolism. Mol. Gen. Genet. 210, 135–139

    Not in our library10.1007/bf00337769

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    Hantke K. (2001 ). Iron and metal regulation in bacteria. Curr. Opin. Microbiol. 4, 172–177

    Not in our library10.1016/s1369-5274(00)00184-3

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    Haraszthy V. I. Jordan S. F. Zambon J. J. (2006 ). Identification of Fur-regulated genes in Actinobacillus actinomycetemcomitans. Microbiology 152, 787–796

    Not in our library10.1099/mic.0.28366-0

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    Harrison A. Santana E. A. Szelestey B. R. Newsom D. E. White P. Mason K. M. (2013 ). Ferric uptake regulator and its role in the pathogenesis of nontypeable Haemophilus influenzae. Infect. Immun. 81, 1221–1233

    Not in our library10.1128/iai.01227-12

  97. 97.

    Hassan H. M. Fridovich I. (1978 ). Regulation of the synthesis of catalase and peroxidase in Escherichia coli. J. Biol. Chem. 253, 6445–6420

    Not in our library

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