Corrigendum: Modular Enzymatic Cascade Synthesis of Nucleotides Using a (d)ATP Regeneration System

Corrigendum on: Fehlau M, Kaspar F, Hellendahl KF, Schollmeyer J, Neubauer P and Wagner A (2020)System. Front. Bioeng. Biotechnol. 8:854. doi: 10.3389/fbioe.2020.00854In the original article, there was an error. We did not receive the expression plasmid of DmdNK from Daniela Ubiali (which is written 2x in the manuscript), but from Prof.A correction has been made to Materials and Methods, General Information.All chemicals and solvents were of analytical grade or higher and purchased, if not stated otherwise, from Sigma-Aldrich (Steinheim, Germany), Carl Roth (Karlsruhe, Germany), TCI Deutschland (Eschborn, Germany), Carbosynth (Berkshire, United Kingdom) or VWR (Darmstadt, Germany).The following natural and modified nucleoside substrates were used: Natural pyrimidine and purine nucleoside substrates were cytidine (Cyd, 1), deoxycytidine (dCyd, 2), adenosine (Ado, 5) and deoxyadenosine (dAdo, 6). Sugar-modified nucleosides were arabinofuranosylcytosine (araCyd, 3) known as the anti-leukemia drug Cytarabine and arabinofuranosyladenine (araAdo, 7), a nucleoside antibiotic isolated from Streptomyces antibioticus. Base-modified nucleoside substrates were 5-fluorocytidine (5F-Cyd, 4) and 2-fluoroadenosine (2F-Ado, 8). Halogenated nucleotides like 5F-CTP (4c) are interesting

Abstract licence: CC BY

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