Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2
Catherine A. Hogan, ChunHong Huang, Malaya K. Sahoo and 7 more
Emerging Infectious Diseases · 2021
doi:10.3201/eid2702.204168 (opens in a new tab)
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Abstract
We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.
Abstract licence: CC BY
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