Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection
Cassandra E. Faux, Katherine E. Arden, Stephen B. Lambert and 5 more
Emerging Infectious Diseases · 2011
doi:10.3201/eid1702.101123 (opens in a new tab)
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Abstract
We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)–specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.
Abstract licence: CC BY
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