Nested Polymerase Chain Reaction for Amplification of the Cryptosporidium Oocyst Wall Protein Gene
Susana Pedraza-Díaz, Corinne Amar, Gordon L. Nichols and 1 more
Emerging Infectious Diseases · 2001
doi:10.3201/eid0701.700049 (opens in a new tab)
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Abstract
We developed a sensitive nested polymerase chain reaction (PCR) procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven drinking water-associated outbreaks and 51 cases from five swimming pool-associated outbreaks, as well as 1,300 sporadic cases.
Abstract licence: CC BY
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