The effect of laparoscopy and laparotomy on hemostasis in experimental colorectal cancer surgery in rats

<b>Background:</b> Nowadays, it is claimed that advanced laparoscopic methods are minimally invasive, provide quick recovery of normal functions in patients and cause less stress in the organism compared to similar open surgery techniques. There is only limited research on 8-OHdG and 8-OHG levels, which are the markers of oxidative stress in both laparoscopic and open surgery. In this experimental study, we investigated the stress level caused by the laparotomic and laparoscopic colon resections in rats with induced colorectal cancer model.<br /> <b>Material and methods</b>: In this study, colon cancer was induced with subcutaneous dimethylhydrazine (DMH) (20mg/kg) in 30 Sprague-Dawley male rats. After 12 weeks, rats were divided into three groups: Group 1 (control group; n=10), Group 2 (laparotomy group; n=10), and Group 2 (laparoscopy group; n=10). Afterward, blood and lung tissue samples were obtained, and 3-NT, 4-HNE, OHdG, PGF2, and NO levels were measured.<br /> <b>Results: </b>There was a statistically significant difference between the groups in terms of 3-NT (ng/ml), which is a biomarker of oxidative stress (p&lt;0.01). After obtaining blood samples from rats, DNA and RNA were isolated, and a statistically significant difference was found between the laparotomy and laparoscopy groups in terms of 8-OHdG and 8-OHG molecules (p&lt;0.01).<br /> <b>Conclusion:</b> Laparoscopy, which initiated a new era in the scientific field, provided various advantages over laparotomy and widely used in colorectal surgery. In addition, it was demonstrated that laparoscopic surgery caused less oxidative stress, had advantageous effects on the antioxidant defense system, and thus superior to laparotomy.

Abstract licence: CC BY

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