Factorial design in isocratic high-performance liquid chromatography of phenolic compounds
Onjia Antonije E., Vasiljević Tatjana M., Čokeša Đuro M. and 1 more
Journal of the Serbian Chemical Society · 2002
doi:10.2298/jsc0211745o (opens in a new tab)
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Abstract
A multifactor optimization strategy was utilized to predict the isocratic HPLC separation of nine phenols. The retention behavior was studied as a function of changing eluent (methanol – acetic acid) composition. The predicted and measured retentions are in rather good agreement. To locate the optimum in the factor space, the normalized resolution product criterion was applied. In virtually every case, the resolution is limited by the separation of the 2-chlorophenol and 2,4-dinitrophenol pair.
Abstract licence: CC BY
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