Development of a simple method to inoculate necrotizing hepatopancreatitis bacterium in Artemia sp.

Different protocols were performed to evaluate their effectiveness to inoculate necrotizing hepatopancreatitis bacterium (NHPB) into Artemia sp. Protocol-A consisted of adding to water 0.2 mL•L-1 of bacterial inoculum composed of infected hepatopancreas (HPNHPB) and glycerol (1:1 w/v). Protocol-B consisted of adding the same inoculum but at a ratio of 2 mL•L-1, whereas protocol C consisted of adding 0.2 mL•L-1, of HPNHPB+glycerol+formulated feed (0.5:0.5:1 w/v/w). Controls for each treatment consisted of similar inoculums but using healthy hepatopancreas. Artemia from A and B treatments were negative for NHPB after four and eight days post-inoculation, whereas Artemia from treatment C resulted as positive to NHPB after four (75% of individuals) and eight (100%) days post-inoculation. The results suggest that the incorporation of formulated feed into the inoculums induced the bacteria’s consumption of Artemia.

Abstract licence: CC BY

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