Cloning of tomato E8 promoter and its analysis in transient assays using Agro-infilteration system

Isolation and characterization of fruit specific promoters is important for manipulation and targeted expression of recombinant proteins in plants. <em>E8</em> is a fruit specific promoter with 2.2 kb length. In this study, a 1.1 kb core part of <em>E8</em> promoter was used. The amplification of the <em>E8</em> promoter was conducted using specific primers and <em>pfu </em>polymerase by Polymerase Chain Reaction (PCR). After sequencing of <em>E8</em> promoter for its confirmation, the CaMV35S promoter in pBI121 binary vector replaced by <em>E8</em> promoter. The recombinant plasmid pBI-E8-GUS was transfered to <em>Agrobacterium tumefaciens</em> LBA4404 by using freeze-thaw method. The specific expression of <em>gus</em> gene in tomato tissue was evaluated using Agro-infiltration method. <em>Agrobacterium</em> harboring pBI121 was used as control. The results showed that <em>gus</em> gene can express specifically in tomato fruits under the control of <em>E8</em> promoter. Therefore, <em>E8</em> promoter can be used for tissue specific expression of recombinant protein in the fruit of tomato plants.

Abstract licence: CC BY

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