Gene expression analysis of OsPP2C5, a candidate protein phosphatase involved in ABA signal transduction, under salt, drought and cold stress in rice

Protein phosphatase 2C family consists of a group of evolutionary conserved serine/threonine phosphatases which play a role in stress signal transduction. A subfamily of these protein phosphatases in Arabidopsis, including ABI1 and ABI2, are known as components of ABA signal transduction pathway. Nine OsPP2C proteins were found in rice (<em>OsPP2C1</em> to <em>OsPP2C9</em>), carrying all the conserved motifs of this subfamily. Among them, only<em> OsPP2C5</em> transcript levels were significantly up-regulated by drought and abscisic acid which is down-regulated by re-watering or ABA removal. In this research, we investigated the effect of drought (180 mM mannitol), salt (100 mM NaCl) and cold (15°C) stresses on <em>OsPP2C5</em> gene expression in 2-days-old seedlings of FL478, IR29 by semi quantitative RT-PCR and Real time PCR methods. The expression levels of <em>OsPP2C5</em> gene in both cultivars were upregulated by cold and drought stresses compared to control and salt treatment. Three alternative transcripts were identified based on the achieved results by semi quantitative RT-PCR and the alignment of the reported sequences for <em>OsPP2C5</em> gene including cDNA and ESTs. The expression of two transcripts amplifiable by the used primers in semi quantitative RT-PCR method were detected in both cultivars while the transcript levels of the bigger transcript was more in IR29 and the transcript levels of the smaller one (original transcript) was higher in FL478 (under drought and cold stress). It seems the transcript type and the expression level is correlated with the cultivar sensitivity to the respected stress.

Abstract licence: CC BY

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