Identification of Callus Induction Potential of 15 Indonesian Rice Genotypes

The callus induction potential of 15 indica rice genotypes from Indonesia was examined in comparison with that of the japonica rice Nipponbare. Callus was induced from embryos of mature seeds and root segments on MS and CI media. There was genotype × medium × explant interaction for inducing white/cream/yellow callus with an organized structure (callus type I and II) and for callus browning, but not for callus induction ability and diameter of callus. Genotypes significantly differed in inducing high quality of calluses depending on medium and explant used. Four indica types, Fatmawati, Ciapus, BP-23 and BP-360-3, had callus induction-related traits similar to those of Nipponbare. These genotypes would be useful for tissue-culture based research and for crop improvement, particularly for genetic transformation. Culturing seed explant on MS was more suitable for callus induction than either root explant on MS or both explants on CI medium.

Abstract licence: CC BY-NC

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