A polymerase chain reaction-based assay to identify genotype F of hepatitis B virus
M.T.B. Moraes, C. Niel, S.A. Gomes
Brazilian Journal of Medical and Biological Research · 1999
doi:10.1590/s0100-879x1999000100006 (opens in a new tab)
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Abstract
We have developed a polymerase chain reaction (PCR) assay which distinguishes genotype F from the other genotypes of hepatitis B virus (HBV). The method was used to characterize HBV strains isolated in urban areas of the Brazilian Amazon. DNA was amplified in 54 of a total of 78 HBsAg-positive serum samples, using universal, non-genotype-specific primers. Only 4 (7.4%) were identified as genotype F by our genotype-specific PCR assay. This proportion is notably lower than that previously reported in Argentina, Venezuela, Peru, and Central America.
Abstract licence: CC BY
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