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Use of Kisser glycerol gelatin to prepare microscope slides of Phlebotomine sandflies (Diptera: Psychodidae)

Vanusa Gonçalves Holanda, Diogo Brunno e Silva Barbosa, Leudimar Aires Pereira and 1 more

Revista da Sociedade Brasileira de Medicina Tropical · 2019

doi:10.1590/0037-8682-0072-2018 (opens in a new tab)

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Abstract

Abstract INTRODUCTION: Appropriate preservation of specimens is important for taxonomic identification. In sandfly research, various methods have been used for slide preparation; however, high cost, low commercial availability, and associated hazards make their use impossible in some studies. Therefore, the efficacy of Kisser glycerol gelatin for sandfly slide preparation was tested. METHODS: Kisser glycerol gelatin, as a substitute for Canada balsam and Berlese’s fluid, was used for mounting sandflies. RESULTS: Forty-two mounted specimens were created and maintained even after 14 months. CONCLUSIONS: Use of Kisser glycerol gelatin is simple and efficient for preparing microscope slides of sandflies.

Abstract licence: CC BY

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