Cryopreservation and Acclimatization of Lycopersicon esculentum (Mill.) Genotypes

<p><span lang="EN-GB" style="font-size: 12.0pt; font-family: 'Garamond Premr Pro',serif">Romanian tomato (<i>Lycopersicon esculentum</i> Mill.) cultivars have been cryopreserved by encapsulation-dehydration and successfully acclimatized to <i>ex vitro</i> growth conditions. Shoot tips were excised from <i>in vitro</i> grown plants then precultured for 24 h in various sucrose concentrations, dehydrated up to 6 h in laminar air flow prior to direct immersion in liquid nitrogen&nbsp;&nbsp; (</span><span lang="EN-GB" style="font-size: 12.0pt; font-family: 'Times New Roman',serif">&#8722;</span><span lang="EN-GB" style="font-size: 12.0pt; font-family: 'Garamond Premr Pro',serif">196</span><span lang="EN-GB" style="font-size: 12.0pt; font-family: 'Times New Roman',serif">°</span><span lang="EN-GB" style="font-size: 12.0pt; font-family: 'Garamond Premr Pro',serif">C) for 24 h. Different parameters have been studied: the effects of osmoprotection and desiccation duration on the regrowth of cryopreserved shoot tips, the effects of various IBA concentrations on rooting and the <i>ex vitro</i> cclimatization of plants recovered from liquid nitrogen. The highest frequency of regrowth (72% cv. ‘Pontica’) was obtained when encapsulated explants were precultured in 0.5 M sucrose and the moisture content (fresh weight basis) of alginate beads was 23%. The highest rooting rates (58% to 77%) for all cultivars were observed for shoots grown on MS medium supplemented with 1.0 mg/l IBA. The rooted plants could be easily acclimatized <i>ex vitro</i> with up to 100% survival.</span></p>

Abstract licence: CC BY

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