An Ancient Yeast for Young Geneticists: A Primer on the Schizosaccharomyces pombe Model System

Abstract

The fission yeast Schizosaccharomyces pombe is an important model organism for the study of eukaryotic molecular and cellular biology. Studies of S. pombe, together with studies of its distant cousin, Saccharomyces cerevisiae, have led to the discovery of genes involved in fundamental mechanisms of transcription, translation, DNA replication, cell cycle control, and signal transduction, to name but a few processes. However, since the divergence of the two species approximately 350 million years ago, S. pombe appears to have evolved less rapidly than S. cerevisiae so that it retains more characteristics of the common ancient yeast ancestor, causing it to share more features with metazoan cells. This Primer introduces S. pombe by describing the yeast itself, providing a brief description of the origins of fission yeast research, and illustrating some genetic and bioinformatics tools used to study protein function in fission yeast. In addition, a section on some key differences between S. pombe and S. cerevisiae is included for readers with some familiarity with budding yeast research but who may have an interest in developing research projects using S. pombe.

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References 196

1. 1.

   Alfa C. Fantes P. Hyams J. McLeod M. Warbrick E., 1993 Experiments with Fission Yeast. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

   Not in our library

2. 2.

   al-Khodairy F. Fotou E. Sheldrick K. S. Griffiths D. J. Lehmann A. R., 1994 Identification and characterization of new elements involved in checkpoint and feedback controls in fission yeast. Mol. Biol. Cell 5: 147–160.

   Not in our library10.1091/mbc.5.2.147 (opens in a new tab)

3. 3.

   Allshire R. C. Ekwall K., 2015 Epigenetic regulation of chromatin states in Schizosaccharomyces pombe. Cold Spring Harb. Perspect. Biol. 7: a018770.

   Not in our library10.1101/cshperspect.a018770 (opens in a new tab)

4. 4.

   Allshire R. C. Javerzat J. P. Redhead N. J. Cranston G., 1994 Position effect variegation at fission yeast centromeres. Cell 76: 157–169.

   Not in our library10.1016/0092-8674(94)90180-5 (opens in a new tab)

5. 5.

   Allshire R. C. Nimmo E. R. Ekwall K. Javerzat J. P. Cranston G., 1995 Mutations derepressing silent centromeric domains in fission yeast disrupt chromosome segregation. Genes Dev. 9: 218–233.

   Not in our library10.1101/gad.9.2.218 (opens in a new tab)

6. 6.

   Aparicio O. M. Billington B. L. Gottschling D. E., 1991 Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae. Cell 66: 1279–1287.

   Not in our library10.1016/0092-8674(91)90049-5 (opens in a new tab)

7. 7.

   Apolinario E. Nocero M. Jin M. Hoffman C. S., 1993 Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies. Curr. Genet. 24: 491–495.

   Not in our library10.1007/bf00351711 (opens in a new tab)

8. 8.
   Lineage-specific loss and divergence of functionally linked genes in eukaryotes · 2000

   Not checked
9. 9.

   Ashburner M. Ball C. A. Blake J. A. Botstein D. Butler H., 2000 Gene Ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat. Genet. 25: 25–29.

   Not in our library10.1038/75556 (opens in a new tab)

10. 10.

    Aves S. J. Tongue N. Foster A. J. Hart E. A., 1998 The essential Schizosaccharomyces pombe cdc23 DNA replication gene shares structural and functional homology with the Saccharomyces cerevisiae DNA43 (MCM10) gene. Curr. Genet. 34: 164–171.

    Not in our library10.1007/s002940050382 (opens in a new tab)

11. 11.

    Bach M. L., 1987 Cloning and expression of the OMP decarboxylase gene URA4 from Schizosaccharomyces pombe. Curr. Genet. 12: 527–534.

    Not in our library10.1007/bf00419562 (opens in a new tab)

12. 12.

    Bähler J. Wu J. Q. Longtine M. S. Shah N. G. McKenzie A. 3rd, 1998 Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14: 943–951.

    Not in our library10.1002/(sici)1097-0061(199807)14:10<943::aid-yea292>3.0.co;2-y (opens in a new tab)

13. 13.

    Balasubramanian M. K. Hirani B. R. Burke J. D. Gould K. L., 1994 The Schizosaccharomyces pombe cdc3+ gene encodes a profilin essential for cytokinesis. J. Cell Biol. 125: 1289–1301.

    Not in our library10.1083/jcb.125.6.1289 (opens in a new tab)

14. 14.

    Barry J. D. Ginger M. L. Burton P. McCulloch R., 2003 Why are parasite contingency genes often associated with telomeres? Int. J. Parasitol. 33: 29–45.

    Not in our library10.1016/s0020-7519(02)00247-3 (opens in a new tab)

15. 15.

    Baumann P. Cech T. R., 2001 Pot1, the putative telomere end-binding protein in fission yeast and humans. Science 292: 1171–1175.

    Not in our library10.1126/science.1060036 (opens in a new tab)

16. 16.

    Beach D. Durkacz B. Nurse P., 1982 Functionally homologous cell cycle control genes in budding and fission yeast. Nature 300: 706–709.

    Not in our library10.1038/300706a0 (opens in a new tab)

17. 17.

    Beach D. Nurse P., 1981 High-frequency transformation of the fission yeast Schizosaccharomyces pombe. Nature 290: 140–142.

    Not in our library10.1038/290140a0 (opens in a new tab)

18. 18.

    Bean D. M. Heimbach J. Ficorella L. Micklem G. Oliver S. G., 2014 esyN: network building, sharing and publishing. PLoS One 9: e106035.

    Not in our library10.1371/journal.pone.0106035 (opens in a new tab)

19. 19.

    Bentley N. J. Holtzman D. A. Flaggs G. Keegan K. S. DeMaggio A., 1996 The Schizosaccharomyces pombe rad3 checkpoint gene. EMBO J. 15: 6641–6651.

    Not in our library

20. 20.

    Bitton D. A. Grallert A. Scutt P. J. Yates T. Li Y., 2011 Programmed fluctuations in sense/antisense transcript ratios drive sexual differentiation in S. pombe. Mol. Syst. Biol. 7: 559.

    Not in our library10.1038/msb.2011.90 (opens in a new tab)

21. 21.

    Boddy M. N. Furnari B. Mondesert O. Russell P., 1998 Replication checkpoint enforced by kinases Cds1 and Chk1. Science 280: 909–912.

    Not in our library10.1126/science.280.5365.909 (opens in a new tab)

22. 22.

    Bowen N. J. Jordan I. K. Epstein J. A. Wood V. Levin H. L., 2003 Retrotransposons and their recognition of pol II promoters: a comprehensive survey of the transposable elements from the complete genome sequence of Schizosaccharomyces pombe. Genome Res. 13: 1984–1997.

    Not in our library10.1101/gr.1191603 (opens in a new tab)

23. 23.

    Bresch C. Muller G. Egel R., 1968 Genes involved in meiosis and sporulation of a yeast. Mol. Gen. Genet. 102: 301–306.

    Not in our library10.1007/bf00433721 (opens in a new tab)

24. 24.

    Burke J. D. Gould K. L., 1994 Molecular cloning and characterization of the Schizosaccharomyces pombe his3 gene for use as a selectable marker. Mol. Gen. Genet. 242: 169–176.

    Not in our library10.1007/bf00391010 (opens in a new tab)

25. 25.

    Cam H. P. Sugiyama T. Chen E. S. Chen X. FitzGerald P. C., 2005 Comprehensive analysis of heterochromatin- and RNAi-mediated epigenetic control of the fission yeast genome. Nat. Genet. 37: 809–819.

    Not in our library10.1038/ng1602 (opens in a new tab)

26. 26.

    Carpy A. Krug K. Graf S. Koch A. Popic S., 2014 Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (fission yeast). Mol. Cell. Proteomics 13: 1925–1936.

    Not in our library10.1074/mcp.m113.035824 (opens in a new tab)

27. 27.

    Carr A. M., 2004 Checkpoint controls halting the cell cycle, pp. 41–56 in The Molecular Biology of Schizosaccharomyces pombe: Genetics, Genomics, and Beyond, edited by Egel R. Springer, Berlin.

    Not in our library

28. 28.

    Chang F. Drubin D. Nurse P., 1997 cdc12p, a protein required for cytokinesis in fission yeast, is a component of the cell division ring and interacts with profilin. J. Cell Biol. 137: 169–182.

    Not in our library10.1083/jcb.137.1.169 (opens in a new tab)

29. 29.

    Chatr-Aryamontri A. Breitkreutz B. J. Heinicke S. Boucher L. Winter A., 2013 The BioGRID interaction database: 2013 update. Nucleic Acids Res. 41: D816–823.

    Not in our library10.1093/nar/gks1158 (opens in a new tab)

30. 30.

    Chen D. Toone W. M. Mata J. Lyne R. Burns G., 2003 Global transcriptional responses of fission yeast to environmental stress. Mol. Biol. Cell 14: 214–229.

    Not in our library10.1091/mbc.e02-08-0499 (opens in a new tab)

31. 31.

    Chien C. T. Bartel P. L. Sternglanz R. Fields S., 1991 The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest. Proc. Natl. Acad. Sci. USA 88: 9578–9582.

    Not in our library10.1073/pnas.88.21.9578 (opens in a new tab)

32. 32.

    Chikashige Y. Hiraoka Y., 2001 Telomere binding of the Rap1 protein is required for meiosis in fission yeast. Curr. Biol. 11: 1618–1623.

    Not in our library10.1016/s0960-9822(01)00457-2 (opens in a new tab)

33. 33.

    Clarke L. Amstutz H. Fishel B. Carbon J., 1986 Analysis of centromeric DNA in the fission yeast Schizosaccharomyces pombe. Proc. Natl. Acad. Sci. USA 83: 8253–8257.

    Not in our library10.1073/pnas.83.21.8253 (opens in a new tab)

34. 34.

    Cooper J. P. Nimmo E. R. Allshire R. C. Cech T. R., 1997 Regulation of telomere length and function by a Myb-domain protein in fission yeast. Nature 385: 744–747.

    Not in our library10.1038/385744a0 (opens in a new tab)

35. 35.

    Costello G. Rodgers L. Beach D., 1986 Fission yeast enters the stationary phase G0 state from either mitotic G1 or G2. Curr. Genet. 11: 119–125.

    Not in our library

36. 36.

    Coxon A. Maundrell K. Kearsey S. E., 1992 Fission yeast cdc21+ belongs to a family of proteins involved in an early step of chromosome replication. Nucleic Acids Res. 20: 5571–5577.

    Not in our library10.1093/nar/20.21.5571 (opens in a new tab)

37. 37.

    Dalgaard J. Z. Klar A. J., 2001 A DNA replication-arrest site RTS1 regulates imprinting by determining the direction of replication at mat1 in S. pombe. Genes Dev. 15: 2060–2068.

    Not in our library10.1101/gad.200801 (opens in a new tab)

38. 38.

    de Medeiros A. S. Kwak G. Vanderhooft J. Rivera S. Gottlieb R., 2015 Fission yeast-based high-throughput screens for PKA pathway inhibitors and activators, pp. 77–91 in Chemical Biology: Methods and Protocols, edited by Hempel J. E. Williams C. H. Hong C. C. Springer, New York.

    Not in our library10.1007/978-1-4939-2269-7_6 (opens in a new tab)

39. 39.

    Doe C. L. Wang G. Chow C. Fricker M. D. Singh P. B., 1998 The fission yeast chromo domain encoding gene chp1(+) is required for chromosome segregation and shows a genetic interaction with alpha-tubulin. Nucleic Acids Res. 26: 4222–4229.

    Not in our library10.1093/nar/26.18.4222 (opens in a new tab)

40. 40.

    Duina A. A. Miller M. E. Keeney J. B., 2014 Budding yeast for budding geneticists: a primer on the Saccharomyces cerevisiae model system. Genetics 197: 33–48.

    Not in our library10.1534/genetics.114.163188 (opens in a new tab)

Cited by papers we hold 2

• PomBase 2018: user-driven reimplementation of the fission yeast database provides rapid and intuitive access to diverse, interconnected information · 2018
  No notice
• CRISPR/Cas system for yeast genome engineering: advances and applications · 2017
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