Capillary Gas Chromatographical Analysis and Mass Spectrometric Identification of Polycyclic Aromatic Hydrocarbon Metabolites from Biological Materials

Supplementary to HPLC, capillary gas chromatography (CGC) can be used in combination with mass spectrometry (MS) for the characterization and identification of polycyclic aromatic hydrocarbon (PAH) metabolites from various biological systems.The main advantage of this technique can be seen in (a) the high resolution of CGC which is superior to HPLC, (b) the predictability of response factors according to the dependence of the FID-signals on the carbon content, and (3) the far-reaching and readily obtainable information on the structure of metabolites by MS.A disadvantage is that volatility of the metabolites is required and that extremely polar compounds (such as glucuronides, sulfates, etc.) cannot be analyzed by this method.These problems can be partially overcome by derivatizing the metabolites.

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