Conference abstracts 1

AIM:Phospholipase A 2 (PLA 2, EC 3.1.1.4) releases fatty acids and lysophospholipids from membrane phospholipids. These secondary mediators are involved in the response to changes of external and internal environment. An example of plant-defensive responses is the accumulation of secondary metabolites such as alkaloids in mechanical damaged tissue. The thesis is focused on studying the impact of 24-hour exposure of a specific PLA 2 inhibitor -aristolochic acid at a concentration of 50 mol.l - on benzylisoquinoline alkaloid (BIAs) biosynthesis. MATERIALS/METHODS: The content of BIAs and mRNA transcription of selected genes (CNMT, 4-OMT and SalAT) involved in their biosynthesis were determined in intact or mechanically wounded 5-week-old poppy seedlings (Papaver somniferum L.). RESULTS: After inhibitor application in intact plants, real-time PCR analysis showed an increase in expression level of CNMT, but after injury, the expression rate was reduced. In uninjured and injured plants incubated with aristolochic acid the expression of 4-OMT and SalAT was decreased. Using LC-MS method, we have determined the level of five poppy alkaloids. After inhibitor application, morphine levels were higher before and after wounding. The level of reticuline, salutaridine and codeine increased in the wounded plant. The amount of thebaine was accumulated to a greater extent after the injury. Based on the results, we assume the existence of several regulatory mechanisms participating in the biosynthesis of secondary metabolites.

Abstract licence: CC BY-NC-ND

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