A high-throughput assay for siRNA-based circadian screens in human U2OS cells.
Christopher Vollmers, Satchidananda Panda, Luciano DiTacchio
PLoS ONE · 2008
doi:10.1371/journal.pone.0003457 (opens in a new tab)
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Abstract
The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination with real-time bioluminescence measurement of a circadian reporter gene, can be utilized to elucidate the effects of gene knockdown across several days in human cells.
Abstract licence: CC BY
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