Recent advances in understanding Pseudomonas aeruginosa as a pathogen

1Pseudomonas aeruginosa is a metabolically versatile ubiquitous gamma-proteobacterium that thrives in soil and aquatic habitats and colonizes the animate surfaces of plants, animals, and humans. P. aeruginosa may cause multiple infections in man that vary from local to systemic and from benign to life threatening. During the last few decades, the cosmopolitan Gram-negative bacterium has become one of the most frequent causative agents of nosocomial infections associated with substantial morbidity and mortality. Pneumonia and sepsis in intensive care unit (ICU) patients still have a bleak prognosis. Chronic airway infections with P. aeruginosa are a major cause of morbidity in people with cystic fibrosis (CF) or chronic obstructive pulmonary disease (COPD) –. Here we report on recent advances in the understanding of host–pathogen interactions with particular emphasis on infections with P. aeruginosa in humans.

2P. aeruginosa is equipped with a large repertoire of virulence determinants ( Table 1) and a complex regulatory network of intracellular and intercellular signals, that allow the bacteria to adapt to and thrive in an animate niche and to escape host defence. This ability, however, varies from clone to clone and from strain to strain in the worldwide P. aeruginosa population. When representative isolates of the 20 most common clones in clinical and environmental habitats ( Figure 1) were tested in three established infection models, i.e. an acute infection of murine airways, lettuce, and wax moth larvae, an unexpected gradient of pathogenicity was observed. Under the conditions of the standardized acute airway infection model in the mouse, the full spectrum of possible host responses to P. aeruginosa was seen that ranged from unimpaired health to 100% lethality. Likewise, similar gradients of virulence were observed with plant and insect hosts whereby the pathogenicity of most strains differed by host. A strain could be innocuous for the mouse but highly pathogenic for the lettuce, and vice versa.

3The differential genetic repertoire of clones maintains a host-specific gradient of virulence in the P. aeruginosa population whereby differential sets of pathogenicity factors and mechanisms are employed to conquer the diverse animate niches. In other words, the fitness of a clone to colonize and to persist differs by habitat. This conclusion is supported by real-world data. Close to 3,000 spatiotemporally unrelated isolates from the environment, acute human infection, and chronically colonized COPD and CF airways were genotyped with a marker microarray yielding 300-odd clone genotypes. The 20 most frequent clones had an absolute share of 44%, indicating that the P. aeruginosa population is dominated by few epidemic clonal lineages. The most abundant clones like C or PA14 were detected in all habitats, albeit at different frequencies. On the other hand, the proportion of habitat-specific clones was 25% in COPD, 32% in acute infections, 39% in the environment, and 54% in CF, indicating that the CF lungs select for rare clones that can withstand exposure to a hostile immune system and regular antimicrobial chemotherapy and that the soil and aquatic habitats accommodate a subgroup of clones that cannot colonize a human niche. The spectrum of clones was broader in COPD and CF lungs than in the multiple niches of acute infections, implying that the airways of a predisposed host can be colonized by more clone types than the organs of a previously healthy and immunocompetent host that suffers from an acute insult. In summary, a human habitat can be colonized by some generalists like clone C or PA14 and some minor clones that have a low prevalence in the global population but are endowed with clone-specific features of fitness and/or pathogenicity to become a dominant member in the particular human niche. Since most research has focused on clinical isolates, the analysis of isolates from non-clinical habitats may reveal features yet unknown for P. aeruginosa.

4The Pseudomonas genome database (20 June 2017) lists 37 non-coding RNAs in the genome of reference strain PAO1. These small RNAs (sRNA) have regulatory functions. Prominent examples are RsmY, RsmZ, or CrcZ, which are master regulators of bacterial lifestyle, biofilm formation, and carbon metabolism –. Recently, the first example of trans-kingdom biological activity of a regulatory P. aeruginosa sRNA was described. P. aeruginosa bacteria released outer membrane vesicles (OMVs) containing thousands of unique sRNA fragments. The OMVs fused with and delivered sRNAs into mammalian cells, thereby attenuating neutrophil recruitment and the secretion of pro-inflammatory cytokines.

5The type III secretion system (T3SS) and its effectors are the major virulence determinants of P. aeruginosa. The T3SS forms a needle that directly injects virulence effectors (ExoS, T, U, and Y) into the host cell. The ADP-ribosyltransferase (ADPRT) ExoS, and the phospholipase A2 ExoU, occur almost mutually exclusively in P. aeruginosa so that the population is currently differentiated into a major ExoS-positive clade, a minor ExoU-positive clade, and a minute T3SS-negative clade lacking both ExoS and ExoU,. ExoU and its homologue PlpD,, which is secreted through the type V secretion system, are lipolytic enzymes of the patatin-like protein family. ExoU is highly cytotoxic and more virulent than ExoS in infection models, which correlates with the higher morbidity of acute infections with ExoU-positive than with ExoS-positive strains. ExoS and ExoT consist of an N-terminal GTPase-activating protein (GAP) domain and a C-terminal ADPRT domain. Both ExoS and ExoT disrupt the signalling pathway responsible for the activation and assembly of the phagocytic NADPH oxidase and thereby block the production of reactive oxygen species ( Table 1). ExoT moreover promotes the apoptosis of host cells. The GAP domain of ExoT triggers the mitochondrial intrinsic pathway of apoptosis, and ADPRT domain activity transforms the focal adhesion adaptor protein Crk of the host cell into a cytotoxin that induces a form of programmed cell death known as anoikis, which occurs in cells when they detach from the surrounding extracellular matrix. The pathogenicity of ExoS, ExoU, and ExoT is well established, but the role of ExoY during infections still needs to be elucidated –. ExoY synthesizes numerous cyclic nucleotides (cNMPs). The uncommon cUMP turned out to be the most prominent cNMP generated in the lungs of mice infected with ExoY overexpressing P. aeruginosa. cUMP was detectable in body fluids, suggesting that this unusual cNMP in contrast to cAMP or cGMP is not rapidly degraded and thus may interfere with the second messenger signalling of the host.

6The T3SS-negative clade represents three groups of taxonomic outliers with above-average sequence diversity,,. This, however, does not imply that these strains are innocuous. Firstly, isolates have transferred genomic islands to major ExoS-positive clonal lineages and thereby supplied the recipients with determinants of resistance to antimicrobials. Secondly, some strains harbour the recently discovered two-partner secretion system ExlAB,. ExlB exports a pore-forming toxin called exolysin (ExlA), which induces plasma membrane rupture in epithelial, endothelial, and immune cells but not in erythrocytes. Thirdly, these outliers, like all other ExoS- or ExoU-positive P. aeruginosa strains, possess type VI secretion systems (T6SS).

7The T6SS is a bacterial nanomachine that shares similarity with the puncturing device of bacteriophages,. The three separate H1-, H2-, and H3-T6SS translocate proteins between cells by a mechanism analogous to phage tail contraction. The H1-T6SS delivers the three toxins Tse1–3, which kill bacterial competitors inhabiting the same niche. The H2- and H3-T6SS target both prokaryotic and eukaryotic cells, i.e. they translocate trans-kingdom effectors –. These toxins exert antibacterial activity and facilitate intracellular invasion of host eukaryotic cells ( Table 1). The phospholipase TplE, for example, disrupts the endoplasmic reticulum and thereby promotes autophagy by the activation of the unfolded protein response, –.

8P. aeruginosa can switch between a motile and a sessile lifestyle and can modulate the secretion of virulence effectors by a plethora of transcription factors, two-component systems, non-coding RNAs, and quorum-sensing networks,. Research in the last few years has focused on the complex signalling pathways mediated by the second messenger cyclic di-GMP (c-di-GMP),. P. aeruginosa possesses more than 40 diguanylate cyclases and c-di-GMP-degrading phosphodiesterases, the specificities of which are currently under investigation,.

9Elevated levels of c-di-GMP promote biofilm formation typical for chronic infections and repress flagellum-driven swarming motility typical for acute infections and vice versa. The master transcription regulator FleQ, for example, activates the transcription of flagellar genes when intracellular c-di-GMP levels are low, but upon binding of c-di-GMP FleQ converts to an activator of the expression of genes involved in biofilm formation such as the adhesin CdrAB or the exopolysaccharides Psl and Pel,. The binding of c-di-GMP leads to major conformational rearrangements in FleQ, which reasonably explains the dual role of FleQ in promoting both the sessile and the motile lifestyles dependent on c-di-GMP levels.

10The third major exopolysaccharide in P. aeruginosa biofilms is alginate, a polymer of mannuronic and guluronic acid. Alginate secretion is promoted by the protein Alg44 upon the binding of two c-di-GMP molecules. The production of alginate is induced under microaerophilic or anaerobic conditions, as is typically the case for chronic infections of COPD and CF lungs. A recently discovered three-gene operon, sadC-odaA-odaI, controls the oxygen-dependent synthesis of alginate. During anaerobiosis, the diguanylate cyclase SadC produces c-di-GMP, but in the presence of oxygen OdaI inhibits c-di-GMP synthesis by SadC. Besides the induction of alginate biosynthesis, SadC inhibits swimming, swarming, and twitching motility, promotes the production of the exopolysaccharide Psl, and is a member of the RetS/LadS/Gac/Rsm regulatory network that constitutes the decision-making switch between sessile and motile lifestyles,.

11The transition from the biofilm to the planktonic growth state is under the complex regulatory control of many other players influencing the intracellular c-di-GMP levels. The chemosensory protein BdlA, the diguanylate cyclase GcbA, and the c-di-GMP phosphodiesterases DipA, RbdA, and NbdA are known proteins that are required for dispersion to occur –.

12P. aeruginosa may persist for decades in the lungs of individuals with CF, which provides the rare opportunity to study the within-host evolution of a bacterial pathogen for an extended period of time. A single case who acquired P. aeruginosa in the neonatal period has already been analysed by whole genome sequencing of serial P. aeruginosa isolates more than 10 years ago. Subsequently, the genetic adaptation of P. aeruginosa to CF lungs has been investigated in carriers of major clones or transmissible lineages – and, more recently, in a cohort of CF children who were followed for the early phase of infection. This study of 474 longitudinal isolates from 34 CF patients seen at the Copenhagen CF clinic, representing 36 different clonal lineages of P. aeruginosa, showed evidence for positive selection at 52 genes, suggesting adaptive evolution to optimise pathogen fitness. Numerous genes encode traits known to be involved in CF lung infection, such as antibiotic resistance or biofilm formation. In our own ongoing work on serial P. aeruginosa isolates from patients seen at the Hannover CF clinic, we rediscovered just a quarter of these 52 candidate pathoadaptive genes to be frequently mutated, demonstrating the versatility of P. aeruginosa to conquer and persist in CF airways. This versatility also shows up in an extensive phenotypic diversity within the P. aeruginosa populations inhabiting CF airways. The analysis of 15 variable traits in 1,720 isolates collected from 10 carriers of the Liverpool epidemic strain revealed 398 unique subtypes. In summary, all of these cross-sectional and longitudinal studies demonstrated extensive genetic and phenotypic diversity of the P. aeruginosa populations in CF lungs –.

13Innate immune defence molecules,, CD95-mediated apoptosis of epithelial cells,, and killing by polymorphonuclear neutrophilic granulocytes (PMNs) play critical roles in fighting P. aeruginosa. PMNs are primarily recruited by chemokines to the diseased microenvironment. Upon chemokine binding, the chemokine receptor CXCR2 mediates the migration of the neutrophil to sites of inflammation and the chemokine receptor CXCR1 stimulates the production of reactive oxygen species to kill the P. aeruginosa bacteria. The clearance of the bacteria, however, should not be accompanied by excessive and systemic inflammation. Recent studies on the cytokines of the interleukin-17 (IL-17) family highlighted this delicate balance between antibacterial host response and inflammation-triggered organ pathology –. Through the use of a chronic murine pulmonary infection model, IL-17 cytokine signalling was found to be essential for mouse survival and the prevention of chronic infection with P. aeruginosa. On the other hand, IL-17A deficiency protected mice from an acute lethal P. aeruginosa lung infection. In the lung, IL-17A is released from numerous T-cell subtypes, innate lymphoid cells, and macrophages. IL-17A recruits inflammatory cells, but it also activates the airway epithelium to produce IL-17C. IL-17C increases the release of neutrophilic cytokines from alveolar epithelium and thus amplifies inflammation. IL-17C production is also directly induced by P. aeruginosa. Thus, epithelial cells activated by both the pathogen and the professional immune cells contribute to local and systemic inflammation during P. aeruginosa infection.

14The IL-17 story demonstrates the context-dependent, subtle balance of harm and benefit of host defence mechanisms against P. aeruginosa. The ongoing race between pathogen and host has also resulted in highly specific host responses to individual bacterial components and vice versa. Some key mechanisms have recently been elucidated.

15The secondary metabolite phenazine, for example, is recognised by the aryl hydrocarbon receptor AhR that triggers the recruitment of PMNs to the site of the bacterial insult, whereas the secondary metabolite pyocyanin induces the release of reactive oxygen species from mitochondria, which promotes the death of PMNs. These key cells of host defence not only eliminate P. aeruginosa by phagocytic killing but also trap and kill the microbes by neutrophilic extracellular traps (NETs), which are made up of DNA as the scaffold and neutrophilic granule components such as neutrophil elastase and myeloperoxidase,. The flagellum has been shown to be the main bacterial organelle that induces NET formation and thus triggers inflammation. Early growth phase P. aeruginosa are the strongest NET inducers. On the other hand, flagellar motility is downregulated and the flagellum is even lost during the course of chronic lung infections so that P. aeruginosa evades NET-mediated killing –.

16If P. aeruginosa conquers a mammalian niche, it delivers the T3SS effector molecule ExoS directly into the cytosol of the host cell. In a mouse pneumonia model, phagocytes were targeted for injection of ExoS early during infection followed by injection of epithelial cells at later time points so that finally the pulmonary–vascular barrier was disrupted. This mechanism of stepwise inactivation of host defence cells first and the epithelial barrier thereafter makes it plausible that P. aeruginosa bacteria in the lung can gain access to the bloodstream and cause sepsis.

17P. aeruginosa has been and still is one of the medically most relevant opportunistic pathogens in man,,. The management of eye and burn wound, infections has made considerable progress, but the acute and chronic,, – pulmonary infections continue to be associated with substantial morbidity and mortality.

18Thanks to the application of omics technologies, we have gained insight into P. aeruginosa’s genome organisation and diversity,,,,, habitat-specific transcriptome –, proteome –, and metabolome – and the co-evolution of P. aeruginosa with competitors in human habitats such as Staphylococcus aureus –. The function of hundreds of previously uncharacterised “conserved hypotheticals” and the structure of secretory nanomachines have been resolved,, – and knowledge has been gained about the complex regulation of the release of exopolysaccharides, secondary metabolites, and virulence effectors. In other words, during the last few years, we have learnt a lot about the modules of bacterial pathogenicity,. In contrast, progress has been rather slow in the field of antipseudomonal host defence. The mechanisms that mediate bacterial clearance without causing excessive immune pathology deserve further investigation if we want to improve the management of Pseudomonas pneumonia and sepsis –. P. aeruginosa is an extremely versatile microorganism, and it will continue to surprise us with as-yet-unappreciated modes of niche adaptation, lifestyle, and pathogenicity.