Wnt inhibitor XAV-939 promote the adipogenic differentiation of dental pulp stem cells
WEN Jun, XU Shuai-mei, LIU Ying
口腔疾病防治 · 2016
doi:10.12016/j.issn.2096-1456.2016.08.005 (opens in a new tab)
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Abstract
Objective To evaluate the effect of Wnt inhibitor XAV-939 on the proliferation and adipogenic differentiation of dental pulp stem cells (DPSCs).Methods The effect of XAV-939 on the proliferation of DPSCs was analyzed by cell counting kit-8(CCK-8). The effect of XAV-939 on the adipogenic differentiation of DPSCs was analyzed by oil red staining. Quantificational real-time polymerase chain reaction (qRT-PCR) was used to analyze the gene expression of glycogen synthase kinase-3β(GSK3β) and β-catenin which were Wnt signaling marker genes, and peroxisome proliferator activated receptor-γ2 (PPARγ2) and CCAAT/enhancer binding protein α(C/EBPα) which were adipogenic marker genes.Results After 14 days, XAV-939 up-regulates expression of GSK3β、PPARγ2 and C/EBPα, while down-regulates β-catenin.Conclusion XAV-939 could promote the adipogenic differentiation of DPSCs by inhibiting Wnt signalling pathway.
Abstract licence: CC BY
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