Enzymatic and non-enzymatic isolation systems for adipose tissue-derived cells: current state of the art

Abstract

In the past decade, adipose tissue became a highly interesting source of adult stem cells for plastic surgery and regenerative medicine. The isolated stromal vascular fraction (SVF) is a heterogeneous cell population including the adipose-derived stromal/stem cells (ASC), which showed regenerative potential in several clinical studies and trials. SVF should be provided in a safe and reproducible manner in accordance with current good manufacturing practices (cGMP). To ensure highest possible safety for patients, a precisely defined procedure with a high-quality control is required. Hence, an increasing number of adipose tissue-derived cell isolation systems have been developed. These systems aim for a closed, sterile, and safe isolation process limiting donor variations, risk for contaminations, and unpredictability of the cell material. To isolate SVF from adipose tissue, enzymes such as collagenase are used. Alternatively, in order to avoid enzymes, isolation systems using physical forces are available. Here, we provide an overview of known existing enzymatic and non-enzymatic adipose tissue-derived cell isolation systems, which are patented, published, or already on the market.

Shown for reading. Its publisher allows quoting it, not republishing it.

References 165

1. 1.

   Coleman WP, Glogau RG, Klein JA, Moy RL, Narins RS, Chuang TY, et al. Guidelines of care for liposuction. J Am Acad Dermatol. 2001;45(3):438-47.

   Not in our library10.1067/mjd.2001.117045

2. 2.

   Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng. 2001;7(2):211-28.

   Not in our library10.1089/107632701300062859

3. 3.

   Harwood HJ. The adipocyte as an endocrine organ in the regulation of metabolic homeostasis. Neuropharmacology. 2012;63(1):57-75.

   Not in our library10.1016/j.neuropharm.2011.12.010

4. 4.

   Coelho M, Oliveira T, Fernandes R. Biochemistry of adipose tissue: an endocrine organ. Arch Med Sci: AMS. 2013;9(2):191-200.

   Not in our library10.5114/aoms.2013.33181

5. 5.

   Minguell JJ, Erices A, Conget P. Mesenchymal stem cells. Exp Biol Med (Maywood, NJ). 2001;226(6):507-20.

   Not in our library10.1177/153537020122600603

6. 6.

   Anker PS I ’t, Scherjon SA, Kleijburg-van der Keur C, de Groot-Swings GM, Claas FH, Fibbe WE, et al. Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta. Stem Cells. 2004;22(7):1338-45.

   Not in our library10.1634/stemcells.2004-0058

7. 7.

   Fukuchi Y, Nakajima H, Sugiyama D, Hirose I, Kitamura T, Tsuji K. Human placenta-derived cells have mesenchymal stem/progenitor cell potential. Stem Cells. 2004;22(5):649-58.

   Not in our library10.1634/stemcells.22-5-649

8. 8.

   De Bari C, Dell’Accio F, Tylzanowski P, Luyten F. Multipotent mesenchymal stem cells from adult human synovial membrane. Arthritis Rheum. 2001;44:1928-42.

   Not in our library10.1002/1529-0131(200108)44:8<1928::aid-art331>3.0.co;2-p

9. 9.

   Young H, Steele T, Bray R, Hudson J, Floyd J, Hawkins K, et al. Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors. Anat Rec. 2001;264:51-62.

   Not in our library10.1002/ar.1128

10. 10.
    Mesenchymal precursor cells in the blood of normal individuals · 2000

    Not checked
11. 11.

    Erices A, Conget P, Minguell JJ. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol. 2000;109(1):235-42.

    Not in our library10.1046/j.1365-2141.2000.01986.x

12. 12.

    Wakitani S, Goto T, Pineda S, Young R, Mansour J, Caplan A, et al. Mesenchymal cell-based repair of large, full-thickness defects of articular cartilage. J Bone Joint Surg Am Vol. 1994;76:579-92.

    Not in our library10.2106/00004623-199404000-00013

13. 13.

    Arai F, Ohneda O, Miyamoto T, Zhang XQ, Suda T. Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation. J Exp Med. 2002;195(12):1549-63.

    Not in our library10.1084/jem.20011700

14. 14.

    Gronthos S, Franklin D, Leddy H, Robey P, Storms R, Gimble J. Surface protein characterization of human adipose tissue-derived stromal cells. J Cell Physiol. 2001;189:54-63.

    Not in our library10.1002/jcp.1138

15. 15.

    Bailo M, Soncini M, Vertua E, Signoroni PB, Sanzone S, Lombardi G, et al. Engraftment potential of human amnion and chorion cells derived from term placenta. Transplantation. 2004;78(10):1439-48.

    Not in our library10.1097/01.tp.0000144606.84234.49

16. 16.

    Pittenger M, Mackay A, Beck S, Jaiswal R, Douglas R, Mosca J, et al. Multilineage potential of adult human mesenchymal stem cells. Science (New York, NY). 1999;284:143-7.

    Not in our library10.1126/science.284.5411.143

17. 17.

    Tse WT, Pendleton JD, Beyer WM, Egalka MC, Guinan EC. Suppression of allogeneic T-cell proliferation by human marrow stromal cells: implications in transplantation. Transplantation. 2003;75(3):389-97.

    Not in our library10.1097/01.tp.0000045055.63901.a9

18. 18.

    Puissant B, Barreau C, Bourin P, Clavel C, Corre J, Bousquet C, et al. Immunomodulatory effect of human adipose tissue-derived adult stem cells: comparison with bone marrow mesenchymal stem cells. Br J Haematol. 2005;129(1):118-29.

    Not in our library10.1111/j.1365-2141.2005.05409.x

19. 19.

    Kubo M, Sonoda Y, Muramatsu R, Usui M. Immunogenicity of human amniotic membrane in experimental xenotransplantation. Invest Ophthalmol Vis Sci. 2001;42(7):1539-46.

    Not in our library

20. 20.

    Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet. 1970;3(4):393-403.

    Not in our library10.1111/j.1365-2184.1970.tb00347.x

21. 21.

    Aust L, Devlin B, Foster SJ, Halvorsen YD, Hicok K, du Laney T, et al. Yield of human adipose-derived adult stem cells from liposuction aspirates. Cytotherapy. 2004;6(1):7-14.

    Not in our library10.1080/14653240310004539

22. 22.

    Cawthorn WP, Scheller EL, MacDougald OA. Adipose tissue stem cells meet preadipocyte commitment: going back to the future. J Lipid Res. 2012;53(2):227-46.

    Not in our library10.1194/jlr.r021089

23. 23.

    Cousin B, Andre M, Arnaud E, Penicaud L, Casteilla L. Reconstitution of lethally irradiated mice by cells isolated from adipose tissue. Biochem Biophys Res Commun. 2003;301(4):1016-22.

    Not in our library10.1016/s0006-291x(03)00061-5

24. 24.

    Han J, Koh YJ, Moon HR, Ryoo HG, Cho CH, Kim I, et al. Adipose tissue is an extramedullary reservoir for functional hematopoietic stem and progenitor cells. Blood. 2010;115(5):957-64.

    Not in our library10.1182/blood-2009-05-219923

25. 25.

    McIntosh K, Zvonic S, Garrett S, Mitchell JB, Floyd ZE, Hammill L, et al. The immunogenicity of human adipose-derived cells: temporal changes in vitro. Stem Cells. 2006;24(5):1246-53.

    Not in our library10.1634/stemcells.2005-0235

26. 26.

    Zimmerlin L, Donnenberg VS, Pfeifer ME, Meyer EM, Peault B, Rubin JP, et al. Stromal vascular progenitors in adult human adipose tissue. Cytometry Part A: J Int Soc Anal Cytol. 2010;77(1):22-30.

    Not in our library10.1002/cyto.a.20813

27. 27.

    Yoshimura K, Asano Y, Aoi N, Kurita M, Oshima Y, Sato K, et al. Progenitor-enriched adipose tissue transplantation as rescue for breast implant complications. Breast J. 2010;16(2):169-75.

    Not in our library10.1111/j.1524-4741.2009.00873.x

28. 28.

    Sterodimas A, de Faria J, Nicaretta B, Boriani F. Autologous fat transplantation versus adipose-derived stem cell-enriched lipografts: a study. Aesthet Surg J. 2011;31(6):682-93.

    Not in our library10.1177/1090820x11415976

29. 29.

    Yoshimura K, Sato K, Aoi N, Kurita M, Hirohi T, Harii K. Cell-assisted lipotransfer for cosmetic breast augmentation: supportive use of adipose-derived stem/stromal cells. Aesthetic Plast Surg. 2008;32(1):48-55.

    Not in our library10.1007/s00266-007-9019-4

30. 30.

    Rigotti G, Marchi A, Galie M, Baroni G, Benati D, Krampera M, et al. Clinical treatment of radiotherapy tissue damage by lipoaspirate transplant: a healing process mediated by adipose-derived adult stem cells. Plast Reconstr Surg. 2007;119(5):1409-22.

    Not in our library10.1097/01.prs.0000256047.47909.71

31. 31.
    Non-expanded adipose stromal vascular fraction cell therapy for multiple sclerosis · 2009

    No notice
32. 32.

    Michalek J, Moster R, Lukac L, Proefrock K, Petrasovic M, Rybar J, et al. Autologous adipose tissue-derived stromal vascular fraction cells application in patients with osteoarthritis. Cell Transplant. 2015.

    Not in our library10.3727/096368915x686760

33. 33.

    Bourin P, Bunnell BA, Casteilla L, Dominici M, Katz AJ, March KL, et al. Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy. 2013;15(6):641-8.

    Not in our library10.1016/j.jcyt.2013.02.006

34. 34.

    Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002;13(12):4279-95.

    Not in our library10.1091/mbc.e02-02-0105

35. 35.

    Gimble J, Guilak F. Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy. 2003;5(5):362-9.

    Not in our library10.1080/14653240310003026

36. 36.

    Ashjian PH, Elbarbary AS, Edmonds B, DeUgarte D, Zhu M, Zuk PA, et al. In vitro differentiation of human processed lipoaspirate cells into early neural progenitors. Plast Reconstr Surg. 2003;111(6):1922-31.

    Not in our library10.1097/01.prs.0000055043.62589.05

37. 37.

    Banerjee A, Nurnberger S, Hennerbichler S, Riedl S, Schuh CM, Hacobian A, et al. In toto differentiation of human amniotic membrane towards the Schwann cell lineage. Cell Tissue Bank. 2014;15(2):227-39.

    Not in our library10.1007/s10561-013-9401-1

38. 38.

    Boquest AC, Shahdadfar A, Fronsdal K, Sigurjonsson O, Tunheim SH, Collas P, et al. Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture. Mol Biol Cell. 2005;16(3):1131-41.

    Not in our library10.1091/mbc.e04-10-0949

39. 39.

    Safford KM, Hicok KC, Safford SD, Halvorsen YD, Wilkison WO, Gimble JM, et al. Neurogenic differentiation of murine and human adipose-derived stromal cells. Biochem Biophys Res Commun. 2002;294(2):371-9.

    Not in our library10.1016/s0006-291x(02)00469-2

40. 40.

    Planat-Benard V, Menard C, Andre M, Puceat M, Perez A, Garcia-Verdugo JM, et al. Spontaneous cardiomyocyte differentiation from adipose tissue stroma cells. Circ Res. 2004;94(2):223-9.

    Not in our library10.1161/01.res.0000109792.43271.47

41. 41.

    Rangappa S, Fen C, Lee EH, Bongso A, Sim EK. Transformation of adult mesenchymal stem cells isolated from the fatty tissue into cardiomyocytes. Ann Thorac Surg. 2003;75(3):775-9.

    Not in our library10.1016/s0003-4975(02)04568-x

42. 42.

    Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A. Improvement of postnatal neovascularization by human adipose tissue-derived stem cells. Circulation. 2004;110(3):349-55.

    Not in our library10.1161/01.cir.0000135466.16823.d0

43. 43.

    Planat-Benard V, Silvestre JS, Cousin B, Andre M, Nibbelink M, Tamarat R, et al. Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives. Circulation. 2004;109(5):656-63.

    Not in our library10.1161/01.cir.0000114522.38265.61

44. 44.

    Rehman J, Traktuev D, Li J, Merfeld-Clauss S, Temm-Grove CJ, Bovenkerk JE, et al. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation. 2004;109(10):1292-8.

    Not in our library10.1161/01.cir.0000121425.42966.f1

45. 45.

    Al Battah F, De Kock J, Vanhaecke T, Rogiers V. Current status of human adipose-derived stem cells: differentiation into hepatocyte-like cells. TheScientificWorldJOURNAL. 2011;11:1568-81.

    Not in our library10.1100/tsw.2011.146

46. 46.

    Seo MJ, Suh SY, Bae YC, Jung JS. Differentiation of human adipose stromal cells into hepatic lineage in vitro and in vivo. Biochem Biophys Res Commun. 2005;328(1):258-64.

    Not in our library10.1016/j.bbrc.2004.12.158

47. 47.

    Baer PC. Adipose-derived stem cells and their potential to differentiate into the epithelial lineage. Stem Cells Dev. 2011;20(10):1805-16.

    Not in our library10.1089/scd.2011.0086

48. 48.

    Brzoska M, Geiger H, Gauer S, Baer P. Epithelial differentiation of human adipose tissue-derived adult stem cells. Biochem Biophys Res Commun. 2005;330(1):142-50.

    Not in our library10.1016/j.bbrc.2005.02.141

49. 49.

    Vossmerbaeumer U, Ohnesorge S, Kuehl S, Haapalahti M, Kluter H, Jonas JB, et al. Retinal pigment epithelial phenotype induced in human adipose tissue-derived mesenchymal stromal cells. Cytotherapy. 2009;11(2):177-88.

    Not in our library10.1080/14653240802714819

50. 50.

    Du Y, Roh DS, Funderburgh ML, Mann MM, Marra KG, Rubin JP, et al. Adipose-derived stem cells differentiate to keratocytes in vitro. Mol Vis. 2010;16:2680-9.

    Not in our library

51. 51.

    Ferro F, Spelat R, Falini G, Gallelli A, D’Aurizio F, Puppato E, et al. Adipose tissue-derived stem cell in vitro differentiation in a three-dimensional dental bud structure. Am J Pathol. 2011;178(5):2299-310.

    Not in our library10.1016/j.ajpath.2011.01.055

52. 52.

    Salgado AJ, Reis RL, Sousa NJ, Gimble JM. Adipose tissue derived stem cells secretome: soluble factors and their roles in regenerative medicine. Curr Stem Cell Res Ther. 2010;5(2):103-10.

    Not in our library10.2174/157488810791268564

53. 53.

    Tobita M, Orbay H, Mizuno H. Adipose-derived stem cells: current findings and future perspectives. Discov Med. 2011;11(57):160-70.

    Not in our library

54. 54.

    Frazier TP, Gimble JM, Kheterpal I, Rowan BG. Impact of low oxygen on the secretome of human adipose-derived stromal/stem cell primary cultures. Biochimie. 2013;95(12):2286-96.

    Not in our library10.1016/j.biochi.2013.07.011

55. 55.

    Moon KM, Park YH, Lee JS, Chae YB, Kim MM, Kim DS, et al. The effect of secretory factors of adipose-derived stem cells on human keratinocytes. Int J Mol Sci. 2012;13(1):1239-57.

    Not in our library10.3390/ijms13011239

56. 56.

    Kutten JC, McGovern D, Hobson CM, Luffy SA, Nieponice A, Tobita K, et al. Decellularized tracheal extracellular matrix supports epithelial migration, differentiation, and function. Tissue Eng A. 2015;21(1–2):75-84.

    Not in our library10.1089/ten.tea.2014.0089

57. 57.

    Hassan WU, Greiser U, Wang W. Role of adipose-derived stem cells in wound healing. Wound repair and regeneration: official publication of the Wound Healing Society [and] the European Tissue Repair. Society. 2014;22(3):313-25.

    Not in our library10.1111/wrr.12173

58. 58.

    Kilroy GE, Foster SJ, Wu X, Ruiz J, Sherwood S, Heifetz A, et al. Cytokine profile of human adipose-derived stem cells: expression of angiogenic, hematopoietic, and pro-inflammatory factors. J Cell Physiol. 2007;212(3):702-9.

    Not in our library10.1002/jcp.21068

59. 59.

    Kronsteiner B, Peterbauer-Scherb A, Grillari-Voglauer R, Redl H, Gabriel C, van Griensven M, et al. Human mesenchymal stem cells and renal tubular epithelial cells differentially influence monocyte-derived dendritic cell differentiation and maturation. Cell Immunol. 2011;267(1):30-8.

    Not in our library10.1016/j.cellimm.2010.11.001

60. 60.

    Wolbank S, Peterbauer A, Fahrner M, Hennerbichler S, van Griensven M, Stadler G, et al. Dose-dependent immunomodulatory effect of human stem cells from amniotic membrane: a comparison with human mesenchymal stem cells from adipose tissue. Tissue Eng. 2007;13(6):1173-83.

    Not in our library10.1089/ten.2006.0313

61. 61.

    Wolbank S, Stadler G, Peterbauer A, Gillich A, Karbiener M, Streubel B, et al. Telomerase immortalized human amnion- and adipose-derived mesenchymal stem cells: maintenance of differentiation and immunomodulatory characteristics. Tissue Eng A. 2009;15(7):1843-54.

    Not in our library10.1089/ten.tea.2008.0205

62. 62.

    Kronsteiner B, Wolbank S, Peterbauer A, Hackl C, Redl H, van Griensven M, et al. Human mesenchymal stem cells from adipose tissue and amnion influence T-cells depending on stimulation method and presence of other immune cells. Stem Cells Dev. 2011;20(12):2115-26.

    Not in our library10.1089/scd.2011.0031

63. 63.

    Cho YB, Park KJ, Yoon SN, Song KH, Kim DS, Jung SH et al. Long-term results of adipose-derived stem cell therapy for the treatment of Crohn’s fistula. Stem Cells Transl Med. 2015. doi:10.5966/sctm.2014-0199.

    Not in our library10.5966/sctm.2014-0199

64. 64.

    Gir P, Oni G, Brown SA, Mojallal A, Rohrich RJ. Human adipose stem cells: current clinical applications. Plast Reconstr Surg. 2012;129(6):1277-90.

    Not in our library10.1097/prs.0b013e31824ecae6

65. 65.

    Mizuno H, Tobita M, Uysal AC. Concise review: adipose-derived stem cells as a novel tool for future regenerative medicine. Stem Cells. 2012;30(5):804-10.

    Not in our library10.1002/stem.1076

66. 66.

    Gimble JM, Guilak F, Bunnell BA. Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells. Stem Cell Res Ther. 2010;1(2):19.

    Not in our library10.1186/scrt19

67. 67.

    Sergeevicheva V, Kruchkova I, Chernykh E, Shevela E, Kulagin A, Gilevich A, et al. Rapid recovery from chronic PRCA by MSC infusion in patient after major ABO-mismatched alloSCT. Case Rep Med. 2012;2012:862721.

    Not in our library10.1155/2012/862721

68. 68.
    Stem cell treatment for patients with autoimmune disease by systemic infusion of culture-expanded autologous adipose tissue derived mesenchymal stem cells · 2011

    No notice
69. 69.

    Bura A, Planat-Benard V, Bourin P, Silvestre JS, Gross F, Grolleau JL, et al. Phase I trial: the use of autologous cultured adipose-derived stroma/stem cells to treat patients with non-revascularizable critical limb ischemia. Cytotherapy. 2014;16(2):245-57.

    Not in our library10.1016/j.jcyt.2013.11.011

70. 70.

    Sandor GK, Numminen J, Wolff J, Thesleff T, Miettinen A, Tuovinen VJ, et al. Adipose stem cells used to reconstruct 13 cases with cranio-maxillofacial hard-tissue defects. Stem Cells Transl Med. 2014;3(4):530-40.

    Not in our library10.5966/sctm.2013-0173

71. 71.

    Thesleff T, Lehtimaki K, Niskakangas T, Mannerstrom B, Miettinen S, Suuronen R, et al. Cranioplasty with adipose-derived stem cells and biomaterial: a novel method for cranial reconstruction. Neurosurgery. 2011;68(6):1535-40.

    Not in our library10.1227/neu.0b013e31820ee24e

72. 72.

    Tanikawa DY, Aguena M, Bueno DF, Passos-Bueno MR, Alonso N. Fat grafts supplemented with adipose-derived stromal cells in the rehabilitation of patients with craniofacial microsomia. Plast Reconstr Surg. 2013;132(1):141-52.

    Not in our library10.1097/prs.0b013e3182910a82

73. 73.

    Zuk P. Adipose-derived stem cells in tissue regeneration: a review. ISRN Stem Cells. 2013;2013:35.

    Not in our library10.1155/2013/713959

74. 74.

    Castro-Malaspina H, Gay RE, Resnick G, Kapoor N, Meyers P, Chiarieri D, et al. Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. Blood. 1980;56(2):289-301.

    Not in our library

75. 75.

    EudraLex. Clinical trial guidelines. 2010.

    Not in our library

76. 76.

    EudraLex. Good manufacturing practice (GMP). 2015.

    Not in our library

77. 77.

    Aarya Hari SG. Production of good manufacturing practice grade equine adiposederived mesenchymal stem cells for therapeutic use. J Stem Cell Res Ther. 2013;03(05):2157-7633.

    Not in our library10.4172/2157-7633.1000154

78. 78.

    Sensebe L, Gadelorge M, Fleury-Cappellesso S. Production of mesenchymal stromal/stem cells according to good manufacturing practices: a review. Stem Cell Res Ther. 2013;4(3):66.

    Not in our library10.1186/scrt217

79. 79.

    Carvalho PP, Gimble JM, Dias IR, Gomes ME, Reis RL. Xenofree enzymatic products for the isolation of human adipose-derived stromal/stem cells. Tissue Eng Part C Meth. 2013;19(6):473-8.

    Not in our library10.1089/ten.tec.2012.0465

80. 80.

    Patrikoski M, Juntunen M, Boucher S, Campbell A, Vemuri MC, Mannerstrom B, et al. Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells. Stem Cell Res Ther. 2013;4(2):27.

    Not in our library10.1186/scrt175

81. 81.

    Aguena M, Fanganiello RD, Tissiani LA, Ishiy FA, Atique R, Alonso N, et al. Optimization of parameters for a more efficient use of adipose-derived stem cells in regenerative medicine therapies. Stem Cells Int. 2012;2012:303610.

    Not in our library10.1155/2012/303610

82. 82.
    High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells · 2011

    Not checked
83. 83.

    Markarian CF, Frey GZ, Silveira MD, Chem EM, Milani AR, Ely PB, et al. Isolation of adipose-derived stem cells: a comparison among different methods. Biotechnol Lett. 2014;36(4):693-702.

    Not in our library10.1007/s10529-013-1425-x

84. 84.

    Philips BJ, Marra KG, Rubin JP. Adipose stem cell-based soft tissue regeneration. Expert Opin Biol Ther. 2012;12(2):155-63.

    Not in our library10.1517/14712598.2012.644533

85. 85.

    Thirumala S, Gimble JM, Devireddy RV. Cryopreservation of stromal vascular fraction of adipose tissue in a serum-free freezing medium. J Tissue Eng Regen Med. 2010;4(3):224-32.

    Not in our library10.1002/term.232

86. 86.

    Guven S, Karagianni M, Schwalbe M, Schreiner S, Farhadi J, Bula S, et al. Validation of an automated procedure to isolate human adipose tissue-derived cells by using the Sepax(R) technology. Tissue Eng Part C Methods. 2012;18(8):575-82.

    Not in our library10.1089/ten.tec.2011.0617

87. 87.

    Lin K, Matsubara Y, Masuda Y, Togashi K, Ohno T, Tamura T, et al. Characterization of adipose tissue-derived cells isolated with the Celution system. Cytotherapy. 2008;10(4):417-26.

    Not in our library10.1080/14653240801982979

88. 88.

    Scherberich A, Galli R, Jaquiery C, Farhadi J, Martin I. Three-dimensional perfusion culture of human adipose tissue-derived endothelial and osteoblastic progenitors generates osteogenic constructs with intrinsic vascularization capacity. Stem Cells. 2007;25(7):1823-9.

    Not in our library10.1634/stemcells.2007-0124

89. 89.

    Lin G, Garcia M, Ning H, Banie L, Guo YL, Lue TF, et al. Defining stem and progenitor cells within adipose tissue. Stem Cells Dev. 2008;17(6):1053-63.

    Not in our library10.1089/scd.2008.0117

90. 90.

    Eom YW, Lee JE, Yang MS, Jang IK, Kim HE, Lee DH, et al. Rapid isolation of adipose tissue-derived stem cells by the storage of lipoaspirates. Yonsei Med J. 2011;52(6):999-1007.

    Not in our library10.3349/ymj.2011.52.6.999

91. 91.

    Suga H, Shigeura T, Matsumoto D, Inoue K, Kato H, Aoi N, et al. Rapid expansion of human adipose-derived stromal cells preserving multipotency. Cytotherapy. 2007;9(8):738-45.

    Not in our library10.1080/14653240701679873

92. 92.

    Safwani WK, Makpol S, Sathapan S, Chua KH. Alteration of gene expression levels during osteogenic induction of human adipose derived stem cells in long-term culture. Cell Tissue Bank. 2013;14(2):289-301.

    Not in our library10.1007/s10561-012-9309-1

93. 93.

    Kirkpatrick CJ, Melzner I, Goller T. Comparative effects of trypsin, collagenase and mechanical harvesting on cell membrane lipids studied in monolayer-cultured endothelial cells and a green monkey kidney cell line. Biochim Biophys Acta. 1985;846(1):120-6.

    Not in our library10.1016/0167-4889(85)90117-x

94. 94.

    Stadler G, Hennerbichler S, Lindenmair A, Peterbauer A, Hofer K, van Griensven M, et al. Phenotypic shift of human amniotic epithelial cells in culture is associated with reduced osteogenic differentiation in vitro. Cytotherapy. 2008;10(7):743-52.

    Not in our library10.1080/14653240802345804

95. 95.

    Kakagia D, Pallua N. Autologous fat grafting: in search of the optimal technique. Surg Innov. 2014;21(3):327-36.

    Not in our library10.1177/1553350613518846

96. 96.

    Garza RM, Paik KJ, Chung MT, Duscher D, Gurtner GC, Longaker MT, et al. Studies in fat grafting: part III. Fat grafting irradiated tissue—improved skin quality and decreased fat graft retention. Plast Reconstr Surg. 2014;134(2):249-57.

    Not in our library10.1097/prs.0000000000000326

97. 97.

    Stillaert FB, Di Bartolo C, Hunt JA, Rhodes NP, Tognana E, Monstrey S, et al. Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds. Biomaterials. 2008;29(29):3953-9.

    Not in our library10.1016/j.biomaterials.2008.06.005

98. 98.

    Matsumoto D, Sato K, Gonda K, Takaki Y, Shigeura T, Sato T, et al. Cell-assisted lipotransfer: supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection. Tissue Eng. 2006;12(12):3375-82.

    Not in our library10.1089/ten.2006.12.3375

99. 99.

    Holnthoner W, Hohenegger K, Husa AM, Muehleder S, Meinl A, Peterbauer-Scherb A, et al. Adipose-derived stem cells induce vascular tube formation of outgrowth endothelial cells in a fibrin matrix. J Tissue Eng Regen Med. 2015;9(2):127-36.

    Not in our library10.1002/term.1620

100. 100.

     Gentile P, Orlandi A, Scioli MG, Di Pasquali C, Bocchini I, Curcio CB, et al. A comparative translational study: the combined use of enhanced stromal vascular fraction and platelet-rich plasma improves fat grafting maintenance in breast reconstruction. Stem Cells Transl Med. 2012;1(4):341-51.

     Not in our library10.5966/sctm.2011-0065

101. 101.

     Jiang A, Li M, Duan W, Dong Y, Wang Y. Improvement of the survival of human autologous fat transplantation by adipose-derived stem-cells-assisted lipotransfer combined with bFGF. TheScientificWorldJOURNAL. 2015;2015:968057.

     Not in our library10.1155/2015/968057

102. 102.

     Luo S, Hao L, Li X, Yu D, Diao Z, Ren L, et al. Adipose tissue-derived stem cells treated with estradiol enhance survival of autologous fat transplants. Tohoku J Exp Med. 2013;231(2):101-10.

     Not in our library10.1620/tjem.231.101

103. 103.

     Li L, Pan S, Ni B, Lin Y. Improvement in autologous human fat transplant survival with SVF plus VEGF-PLA nano-sustained release microspheres. Cell Biol Int. 2014;38(8):962-70.

     Not in our library10.1002/cbin.10284

104. 104.

     Van Pham P, Hong-Thien Bui K, Quoc Ngo D, Tan Khuat L, Kim PN. Transplantation of nonexpanded adipose stromal vascular fraction and platelet-rich plasma for articular cartilage injury treatment in mice model. J Med Eng. 2013;2013:7.

     Not in our library10.1155/2013/832396

105. 105.

     Paspaliaris B, Thornton JAF. Methods and apparatuses for isolating and preparing stem cells. 2014.

     Not in our library

106. 106.

     Tzouvelekis A, Koliakos G, Ntolios P, Baira I, Bouros E, Oikonomou A, et al. Stem cell therapy for idiopathic pulmonary fibrosis: a protocol proposal. J Transl Med. 2011;9:182.

     Not in our library10.1186/1479-5876-9-182

107. 107.
     A prospective, non-randomized, no placebo-controlled, phase Ib clinical trial to study the safety of the adipose derived stromal cells-stromal vascular fraction in idiopathic pulmonary fibrosis · 2013

     No notice
108. 108.

     Aronowitz JA, Ellenhorn JD. Adipose stromal vascular fraction isolation: a head-to-head comparison of four commercial cell separation systems. Plast Reconstr Surg. 2013;132(6):932e-9.

     Not in our library10.1097/prs.0b013e3182a80652

109. 109.

     Kakudo N, Tanaka Y, Morimoto N, Ogawa T, Kushida S, Hara T, et al. Adipose-derived regenerative cell (ADRC)-enriched fat grafting: optimal cell concentration and effects on grafted fat characteristics. J Transl Med. 2013;11:254.

     Not in our library10.1186/1479-5876-11-254

110. 110.

     Granel B, Daumas A, Jouve E, Harle JR, Nguyen PS, Chabannon C, et al. Safety, tolerability and potential efficacy of injection of autologous adipose-derived stromal vascular fraction in the fingers of patients with systemic sclerosis: an open-label phase I trial. Ann Rheum Dis. 2014.

     Not in our library10.1136/annrheumdis-2014-205681

111. 111.

     Gotoh M, Yamamoto T, Kato M, Majima T, Toriyama K, Kamei Y, et al. Regenerative treatment of male stress urinary incontinence by periurethral injection of autologous adipose-derived regenerative cells: 1-year outcomes in 11 patients. Int J Urol. 2014;21(3):294-300.

     Not in our library10.1111/iju.12266

112. 112.

     Perez-Cano R, Vranckx JJ, Lasso JM, Calabrese C, Merck B, Milstein AM, et al. Prospective trial of adipose-derived regenerative cell (ADRC)-enriched fat grafting for partial mastectomy defects: the RESTORE-2 trial. Eur J Surg Oncol. 2012;38(5):382-9.

     Not in our library10.1016/j.ejso.2012.02.178

113. 113.

     Dos-Anjos Vilaboa S, Navarro-Palou M, Llull R. Age influence on stromal vascular fraction cell yield obtained from human lipoaspirates. Cytotherapy. 2014;16(8):1092-7.

     Not in our library10.1016/j.jcyt.2014.02.007

114. 114.

     Pak J, Lee JH, Lee SH. Regenerative repair of damaged meniscus with autologous adipose tissue-derived stem cells. BioMed Res Int. 2014;2014:436029.

     Not in our library10.1155/2014/436029

115. 115.
     Safety reporting on implantation of autologous adipose tissue-derived stem cells with platelet-rich plasma into human articular joints · 2013

     No notice
116. 116.

     Khan ZA, Dulgar-Tulloch AJ, Rakuff S, Shoemaker PA, Kvam EL, Chen X, et al. Automated systems and methods for isolating regenerative cells from adipose tissue. 2012.

     Not in our library

117. 117.

     Kaengkan P, Baek SE, Kim JY, Kam KY, Do BR, Lee ES, et al. Administration of mesenchymal stem cells and ziprasidone enhanced amelioration of ischemic brain damage in rats. Mol Cells. 2013;36(6):534-41.

     Not in our library10.1007/s10059-013-0235-2

118. 118.

     Stubbers R, Coleman ME. Apparatus and methods for cell isolation. 2015.

     Not in our library

119. 119.

     Doi K, Tanaka S, Iida H, Eto H, Kato H, Aoi N, et al. Stromal vascular fraction isolated from lipo-aspirates using an automated processing system: bench and bed analysis. J Tissue Eng Regen Med. 2013;7(11):864-70.

     Not in our library10.1002/term.1478

120. 120.

     Domenis R, Lazzaro L, Calabrese S, Mangoni D, Gallelli A, Bourkoula E, et al. Adipose tissue derived stem cells: in vitro and in vivo analysis of a standard and three commercially available cell-assisted lipotransfer techniques. Stem Cell Res Ther. 2015;6(1):2.

     Not in our library10.1186/scrt536

121. 121.

     Schafer ME, Hicok KC, Mills DC, Cohen SR, Chao JJ. Acute adipocyte viability after third-generation ultrasound-assisted liposuction. Aesthet Surg J Am Soc Aesthet Plast Surg. 2013;33(5):698-704.

     Not in our library10.1177/1090820x13485239

122. 122.

     Zhu M, Cohen SR, Hicok KC, Shanahan RK, Strem BM, Yu JC, et al. Comparison of three different fat graft preparation methods: gravity separation, centrifugation, and simultaneous washing with filtration in a closed system. Plast Reconstr Surg. 2013;131(4):873-80.

     Not in our library10.1097/prs.0b013e31828276e9

123. 123.

     Ansorge H, Garza JR, McCormack MC, Leamy P, Roesch S, Barere A, et al. Autologous fat processing via the Revolve system: quality and quantity of fat retention evaluated in an animal model. Aesthet Surg J Am Soc Aesthet Plast Sur. 2014;34(3):438-47.

     Not in our library10.1177/1090820x14524416

124. 124.

     Dos-Anjos Vilaboa S, Llull R, Mendel TA. Returning fat grafts to physiologic conditions using washing. Plast Reconstr Surg. 2013;132(2):323e-6.

     Not in our library10.1097/prs.0b013e3182958be1

125. 125.

     Zimmerlin L, Rubin JP, Pfeifer ME, Moore LR, Donnenberg VS, Donnenberg AD. Human adipose stromal vascular cell delivery in a fibrin spray. Cytotherapy. 2013;15(1):102-8.

     Not in our library10.1016/j.jcyt.2012.10.009

126. 126.

     Ferguson RE, Cui X, Fink BF, Vasconez HC, Pu LL. The viability of autologous fat grafts harvested with the LipiVage system: a comparative study. Ann Plast Surg. 2008;60(5):594-7.

     Not in our library10.1097/sap.0b013e31817433c5

127. 127.

     Bianchi F, Maioli M, Leonardi E, Olivi E, Pasquinelli G, Valente S, et al. A new nonenzymatic method and device to obtain a fat tissue derivative highly enriched in pericyte-like elements by mild mechanical forces from human lipoaspirates. Cell Transplant. 2013;22(11):2063-77.

     Not in our library10.3727/096368912x657855

128. 128.

     García-Contreras M, Messaggio F, Jimenez O, Mendez A. Differences in exosome content of human adipose tissue processed by non-enzymatic and enzymatic methods. CellR4. 2015;3(1):e1423.

     Not in our library

129. 129.

     Bianchi F, Olivi E, Baldassarre M, Giannone FA, Laggetta M, Valente S, et al. Lipogems, a new modality of fat tissue handling to enhance tissue repair in chronic hind limb ischemia. CellR4. 2014;2(6):e1289.

     Not in our library

130. 130.

     Maioli M, Rinaldi S, Santaniello S, Castagna A, Pigliaru G, Delitala A, et al. Radioelectric asymmetric conveyed fields and human adipose-derived stem cells obtained with a nonenzymatic method and device: a novel approach to multipotency. Cell Transplant. 2014;23(12):1489-500.

     Not in our library10.3727/096368913x672037

131. 131.

     Carelli S, Messaggio F, Canazza A, Hebda DM, Caremoli F, Latorre E, et al. Characteristics and properties of mesenchymal stem cells derived from micro-fragmented adipose tissue. Cell Transplant. 2014.

     Not in our library10.3727/096368914x681603

132. 132.

     Raffaini M, Tremolada C. Micro fractured and purified adipose tissue graft (Lipogems®) can improve the orthognathic surgery outcomes both aesthetically and in postoperative healing. CellR4. 2014;2(0):e1118.

     Not in our library

133. 133.

     Benzi R, Marfia G, Bosetti M, Beltrami G, Magri AS, Versari S et al. Microfractured lipoaspirate may help oral bone and soft tissue regeneration: a case report. CellR4. 2015;3(3):e1583.

     Not in our library

134. 134.

     Giori A, Tremolada C, Vailati R, Navone SE, Marfia G, Caplan AI. Recovery of function in anal incontinence after micro-fragmented fat graft (Lipogems®) injection: two years follow up of the first 5 cases. CellR4. 2015;3(2):e1544.

     Not in our library

135. 135.

     Hu CB, Myers KE, Peterson RC. Devices for harvesting and homogenizing adipose tissue containing autologous endothelial cells. 2000.

     Not in our library

136. 136.

     Victor S. Isolation of stromal vascular fraction from adipose tissue obtained from postmortem source using ultrasonic cavitation. 2014.

     Not in our library

137. 137.

     Bright R, Bright P, Hansen B, Thomas W. Isolation of stem cells from adipose tissue by ultrasonic cavitation, and methods of use. 2014.

     Not in our library

138. 138.

     Bright R, Bright M, Bright P, Hayne S, Thomas WD. Migraine and tension-type headache treated with stromal vascular fraction: a case series. J Med Case Rep. 2014;8:237.

     Not in our library10.1186/1752-1947-8-237

139. 139.

     Schafer ME. Selective lysing of cells using ultrasound. 2013.

     Not in our library

140. 140.

     Gimble JM, Shah FS, Wu X. Non-enzymatic method for isolating human adipose-derived stromal stem cells. 2014.

     Not in our library10.1016/j.jcyt.2013.04.001

141. 141.

     Michalek J. Method for isolation of adipose tissue-derived stromal vascular fraction cells. 2014.

     Not in our library

142. 142.

     Hicok KC, Hedrick MH. Automated isolation and processing of adipose-derived stem and regenerative cells. Methods Mol Biol (Clifton, NJ). 2011;702:87-105.

     Not in our library10.1007/978-1-61737-960-4_8

143. 143.

     Peterson A, Fornace L. Systems, methods and compositions for optimizing tissue and cell enriched grafts. 2010.

     Not in our library

144. 144.

     Hedrick MH, Fraser JK, Schulzki MJ, Byrnes B, Carlson G, Schreiber RE, et al. Systems and methods for isolating and using clinically safe adipose derived regenerative cells. 2012.

     Not in our library

145. 145.

     Kakudo N, Morimoto N, Ogawa T, Kusumoto K. Potential of adipose-derived stem cells for regeneration medicine: clinical application and usefulness of fat grafting. J Stem Cell Res Ther. 2014;4(204):2.

     Not in our library

146. 146.

     Sanchez PL, Sanz-Ruiz R, Fernandez-Santos ME, Fernandez-Aviles F. Cultured and freshly isolated adipose tissue-derived cells: fat years for cardiac stem cell therapy. Eur Heart J. 2010;31(4):394-7.

     Not in our library10.1093/eurheartj/ehp403

147. 147.

     Cimino WW, Llull R, Katz AJ. Tissue processing apparatus and method for processing adipose tissue. 2015.

     Not in our library

148. 148.

     Llull R, Katz AJ, Cimino WW. Method for processing adipose tissue and processing apparatus. 2013.

     Not in our library

149. 149.

     Do BR, Lee JK, Kim JH, Pak SH, Shin BS. Peristaltic pump, and regenerative cell extraction system using same. 2013.

     Not in our library

150. 150.

     Raj SS, Gopal V, Priya N, Krishnegowda B, Thiruvampattil P, Majumdar AS, et al. System for isolating stromal vascular fraction (svf) cells from the adipose tissue and a method thereof. 2015.

     Not in our library

151. 151.

     Williams SK, Kosnik P, England C, Cannon TF, Vossman E, Boland E, et al. Apparatus and methods for preparing tissue grafts. 2007.

     Not in our library

152. 152.

     Wolters R, Yang A, NELSON J, Williams SK, et al. Hand-held micro-liposuction adipose harvester, processor, and cell concentrator. 2014.

     Not in our library

153. 153.

     Ariff GD, Cannon T, Case JL, Haller CL, Kosnik P, Luddy CP, et al. Cell separation apparatus and methods of use. 2008.

     Not in our library

154. 154.

     SHIGEKI S, KIAT OW. Method for isolating stromal vascular fraction. 2015.

     Not in our library

155. 155.

     Buss B. Autologous tissue harvesting and irrigation device. 2012.

     Not in our library

156. 156.

     Kensy A, Winkler KW. Procedure and device for separating adult stem cells from fatty tissue. 2014.

     Not in our library

157. 157.

     Winkler KW, Matthiesen ID. Device for separating adult stem cells. 2013.

     Not in our library

158. 158.

     Matthiesen I, Winkler KW. Vorrichtung zum Separieren von adulten Stammzellen. 2013.

     Not in our library

159. 159.

     Victor S. Ultrasonic cavitation derived stromal or mesenchymal vascular extracts and cells derived therefrom obtained from adipose tissue and use thereof. 2013.

     Not in our library

160. 160.

     Victor S. Isolation of stromal vascular fraction from vascular tissues. 2014.

     Not in our library

161. 161.

     Cimino WW, Katz AJ, Llull R. Apparatus and methods relating to collecting and processing human biological material containing adipose. 2012.

     Not in our library

162. 162.

     Vossman E, Iwami S, Yang A, Cannon T, Paek HJ. Adipose tissue collection and pre-processing devices for use in liposuction procedure. 2014.

     Not in our library

163. 163.

     Tremolada C. Device and method for preparing tissue, particularly adipose tissue. 2013.

     Not in our library

164. 164.

     Chapman JR, Sparks R. Apparatus for centrifugation and methods therefore. 2013.

     Not in our library

165. 165.

     Shah FS, Wu X, Dietrich M, Rood J, Gimble JM. A non-enzymatic method for isolating human adipose tissue-derived stromal stem cells. Cytotherapy. 2013;15(8):979-85.

     Not in our library10.1016/j.jcyt.2013.04.001

Cited by papers we hold 3

• Genetic Stability of Mesenchymal Stromal Cells for Regenerative Medicine Applications: A Fundamental Biosafety Aspect · 2019
  No notice
• Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation · 2017
  No notice
• Adipose Tissue and Mesenchymal Stem Cells: State of the Art and Lipogems® Technology Development · 2016
  No notice

Reads

A person counts once a day per paper. We keep no cookie and no address: a reader is a hash that changes every day. Any month under five reads shows no number.