Up-regulation and clinical relevance of novel helicase homologue <it>DHX32 </it>in colorectal cancer

<p>Abstract</p> <p>Background</p> <p>This study aimed to find novel biomarkers for colorectal cancer.</p> <p>Methods</p> <p>Fluorescent mRNA differential display PCR (DD-PCR) was used to screen the genes differentially expressed in colorectal cancer tissues and their adjacent tissues. The differentially expressed genes were confirmed by real-time PCR and then their clinical relevance (such as association with tumor location and lymph gland metastasis) was further investigated.</p> <p>Results</p> <p>We identified by DD-PCR a novel RNA helicase, <it>DHX32</it>, which showed higher expression in colorectal cancer tissues than their adjacent tissues, and this result was confirmed by real time RT-PCR. In addition, we found that the level of <it>DHX32 </it>gene expression in colorectal cancer was significantly associated with cancer location, lymph gland metastasis, cancer nodal status, differentiation grade, and Dukes, stage.</p> <p>Conclusion</p> <p><it>DHX32 </it>may play an important role in the development of colorectal cancer and could serve as a novel biomarker for colorectal cancer after additional investigation.</p>

Abstract licence: CC BY

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